Review
Biotechnology & Applied Microbiology
Zhongdi Song, Qunfeng Zhang, Wenhui Wu, Zhongji Pu, Haoran Yu
Summary: The strategy of rational design is to predict potential mutants based on the understanding of protein structure-function relationships and introduce mutations using site-directed mutagenesis. Rational design methods are universal, fast, and have the potential for quantitative prediction of designed sequence performance. Designing enzymes with improved activity or selectivity is challenging due to the complexity of enzyme structure and limited understanding of structure-function relationships. However, with computational advancement and deeper understanding of enzyme catalysis, rational design can simplify enzyme engineering and its application is increasing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Guangcai Xu, Andreas Kunzendorf, Michele Crotti, Henriette J. Rozeboom, Andy-Mark W. H. Thunnissen, Gerrit J. Poelarends
Summary: This study used gene duplication and fusion strategy to evolve a promiscuous homohexameric enzyme into an efficient catalyst with high catalytic activity for enantioselective reactions. Through directed evolution and structural analysis, the asymmetric features of the protein were revealed, suggesting that this strategy may become an important technique in enzyme engineering.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Chemistry, Multidisciplinary
Emily Radley, John Davidson, Jake Foster, Richard Obexer, Elizabeth L. Bell, Anthony P. Green
Summary: The development and implementation of engineered enzymes and biocatalytic technologies are crucial for achieving sustainable development goals and protecting the environment. By using directed evolution and other methods, engineered enzymes can improve the sustainability of processes such as carbon capture and utilization, bioremediation, plastic deconstruction, and renewable feedstock generation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Eric J. Ma, Elina Siirola, Charles Moore, Arkadij Kummer, Markus Stoeckli, Michael Faller, Caroline Bouquet, Fabian Eggimann, Mathieu Ligibel, Dan Huynh, Geoffrey Cutler, Luca Siegrist, Richard A. Lewis, Anne-Christine Acker, Ernst Freund, Elke Koch, Markus Vogel, Holger Schlingensiepen, Edward J. Oakeley, Radka Snajdrova
Summary: This study evaluated machine-directed evolution as an enzyme engineering strategy and found that within one cycle, it yielded a library of high-activity mutants with a significantly different activity distribution compared to traditional directed evolution. Structure-guided analysis suggested that linear additivity might provide a simple explanation for the effectiveness of machine-directed evolution in accessing chiral molecules.
Article
Chemistry, Physical
Shinya Ariyasu, Kai Yonemura, Chie Kasai, Yuichiro Aiba, Hiroki Onoda, Yuma Shisaka, Hiroshi Sugimoto, Takehiko Tosha, Minoru Kubo, Takashi Kamachi, Kazunari Yoshizawa, Osami Shoji
Summary: This study reports the catalytic oxidation of methane to methanol by wild-type P450BM3 without mutagenesis. The findings have significant implications for the development of biological methane oxidation technology.
Article
Agriculture, Multidisciplinary
Zhihua Nie, Pi Liu, Yu Wang, Xuan Guo, Zijian Tan, Jie Shen, Zijing Tang, Jianping Lin, Jibin Sun, Ping Zheng, Leilei Zhu
Summary: This study focused on protein engineering of the mechanosensitive glutamate exporter MscCG2 to improve glutamate excretion efficiency, using directed evolution and computer-assisted design. The engineered variants showed increased levels of glutamate excretion and enhanced efflux of 4-fluoroglutamate, with structural analysis revealing changes in the size of MscCG2 portals and inner channel. These findings demonstrate the potential of protein engineering in enhancing bioproduction through improved secretion efficiency of exporters.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Physical
Guillermo Garcia-Marquina, Reyes Nunez-Franco, Francesca Peccati, Yi Tang, Gonzalo Jimenez-Oses, Fernando Lopez-Gallego
Summary: This study reveals that mutation clusters in the acyltransferase LovD9 can enhance enzyme kinetics and stability, providing a theoretical basis for simvastatin synthesis. By combining rational mutation clusters, we successfully generated the variant LovD-BuCh2 with catalytic efficiency close to LovD9.
Review
Chemistry, Physical
Braun Markus, Gruber C. Christian, Krassnigg Andreas, Kummer Arkadij, Lutz Stefan, Oberdorfer Gustav, Siirola Elina, Snajdrova Radka
Summary: This article introduces the application of emerging computational tools in the field of protein engineering, emphasizing the importance of technologies such as artificial intelligence and machine learning. It showcases the use of these tools in the pharmaceutical industry and discusses innovative approaches and future opportunities in protein science. It also critically assesses the limitations and challenges of these technologies.
Article
Biochemistry & Molecular Biology
Wei Xia, Yingguo Bai, Pengjun Shi
Summary: By analyzing and designing the active center of Bgl3A, this study found that enhancing hydrophobic interactions and hydrogen-bonding networks can strengthen substrate binding, resulting in four dominant mutants with significantly lower K-m values and higher catalytic efficiencies. These findings may serve as a valuable reference for the design of other beta-glucosidases and glycoside hydrolases.
Review
Biotechnology & Applied Microbiology
Haoran Yu, Shuang Ma, Yiwen Li, Paul A. Dalby
Summary: Directed evolution is a powerful strategy to engineer protein properties, and hot spot prediction is crucial for producing smart libraries. Selection of hot spots based on sequence and structure allows for efficient generation of proteins with desired properties.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Yi Shuang Wang, Wan Zheng, Nan Jiang, Yun Xia Jin, Zi Kang Meng, Meng Xin Sun, Yu Liang Zong, Tong Xu, Jiapeng Zhu, Ren Xiang Tan
Summary: This study discovered a new class of enzymes called ChaP that can catalyze the deconstruction of intradiol-like o-quinones and release CO2. Experimental evidence showed that by mutating and replacing specific residues, the enzyme's reaction trajectory and regioselectivity can be altered. The results of this study highlight the catalytic versatility and enzymatic plasticity of VOC enzymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Saurabh Rajendra Nirantar
Summary: Enzymes play a crucial role in biotransformations, however, they often need to be engineered for better performance in biotechnological applications. Researchers have developed a variety of wet-lab methods over the years to achieve this goal, which are examined in this review.
Article
Chemistry, Physical
Yunbin Wu, Rui Xu, Yuxin Feng, Heng Song
Summary: Natural products and their derivatives are a promising source for drug discoveries, but their advancement to in vivo drug testing faces challenges. In this study, a immobilized enzyme cascade was developed for the scalable continuous production of neuropharmaceutical prodrugs. This method achieved high yields and demonstrated the applicability of synthetic biology strategies and immobilization biotechnology. Furthermore, animal experiments showed the potential of these prodrugs as antidepressants. This research is highly significant for facilitating the development or discovery of drug candidates and will be welcomed by academic and industrial researchers.
Article
Chemistry, Multidisciplinary
Ludwig L. Bengel, Benjamin Aberle, Alexander-N. Egler-Kemmerer, Samuel Kienzle, Bernhard Hauer, Stephan C. Hammer
Summary: Selective alkylation of pyrazoles using a catalyst-controlled enzymatic system allows for the efficient synthesis of important molecules with unprecedented regioselectivity. The use of a computational enzyme-library design tool enabled the conversion of a promiscuous enzyme into a small enzyme family of pyrazole-alkylating enzymes in one round of mutagenesis and screening. This enzymatic system demonstrates high selectivity and achieves different alkylations of pyrazoles with remarkable regiodivergence.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biotechnology & Applied Microbiology
Shangshang Sun, Chun You
Summary: Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with the potential to convert common table sugar into products with attractive properties. Most DSPs have strict stereo- and regiospecificities, making them useful in synthesizing specific disaccharides. Protein engineering of DSPs is currently being pursued in various laboratories to create a toolbox of new catalysts for glycoside synthesis.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Katya M. Aguilar-Perez, Gustavo Ruiz-Pulido, Dora Medina, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: This article introduces the prospects and applications of nanotechnology in the food industry, including improving food quality, nutritional value, and food safety, as well as the application of nanomaterials in nutraceutical delivery, obesity treatment, and improving the palatability of healthy foods.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Physical
Komal Rizwan, Muhammad Bilal, Yassine Slimani, Pau Loke Show, Sami Rtimi, Arpita Roy, Hafiz M. N. Iqbal
Summary: Increasing global concern over hazardous pollutants in water bodies due to their toxicity and threat to the environment has led to the need for new or upgraded remediation methods. Advanced oxidation processes (AOPs) utilizing highly reactive oxidizing radicals like center dot O and center dot OH have shown efficacy in the mineralization of dye pollutants. Sonolysis, a newer AOP that employs ultrasound irradiation for generating oxidizing radicals, has become a robust hybrid AOP for degrading environmental contaminants. This review focuses on the degradation of dyes through ultrasound-based AOPs and discusses different sono-based methods and their efficacy for wastewater treatment.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Review
Chemistry, Physical
Wenqian Li, Muhammad Bilal, Anil Kumar Singh, Farooq Sher, S. Salman Ashraf, Marcelo Franco, Juliana Heloisa Pine Americo-Pinheiro, Hafiz M. N. Iqbal
Summary: This article reviews protein engineering approaches that focus on regulating the catalytic microenvironment of enzymes. By adjusting pH, creating water-like microenvironments, and activating enzyme catalysis in organic solvents and gas phase, the effectiveness of catalysts can be optimized, making them important for industrial bioprocesses.
Article
Environmental Sciences
Marise Silva de Carvalho, Luiz Henrique Sales de Menezes, Adriana Bispo Pimentel, Floriatan Santos Costa, Polyane Cabral Oliveira, Marta Maria Oliveira dos Santos, Iasnaia Maria de Carvalho Tavares, Muhammad Irfan, Muhammad Bilal, Joao Carlos Teixeira Dias, Julieta Rangel de Oliveira, Marcelo Franco
Summary: In this study, a comprehensive investigation was conducted on the production of xylanase by solid-state fermentation using Aspergillus oryzae ATCC 10124. Enzyme production was optimized through statistical designs for both the mixture of waste residues and fermentation parameters. The optimized process resulted in a significant increase (up to 165%) in enzymatic activity. The xylanase produced in this fermentation process exhibited alkaline characteristics and high thermal stability. Moreover, the multienzyme extract obtained directly from the fermentation process showed efficient saccharification capabilities without the need for enzyme purification.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Arjumand Riaz, Umme Kalsoom, Haq Nawaz Bhatti, Teofil Jesionowski, Muhammad Bilal
Summary: Dye-contaminated toxic waste is a significant environmental problem, but peroxidase from lemon peels has been found to efficiently degrade dyes. In this study, the enzyme successfully degraded two dyes with environmental and cost advantages.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Tehreem Mahmood, Nazim Hussain, Areej Shahbaz, Sikandar Mulla, Hafiz M. N. Iqbal, Muhammad Bilal
Summary: The worldwide depletion of fossil fuel reserves and the associated environmental and health risks are discussed in this study. The need for alternative renewable energy sources, with a focus on algae-based biofuels, is emphasized. Different cultivation systems for algae and various factors affecting biomass yield and biofuel production are outlined. The economic benefits of algae-based biofuels are highlighted.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Umme Kalsoom, Haq Nawaz Bhatti, Kiran Aftab, Faiza Amin, Teofil Jesionowski, Muhammad Bilal
Summary: This study investigated the potential of Brassica oleracea peroxidase in degrading reactive textile dyes Remazol Turquoise Blue 133 G and Drim Red CL4BN. The results showed that optimal conditions for enzymatic decolorization of these dyes were achieved with specific pH, temperature, enzyme activity, hydrogen peroxide concentration, and dye concentration. The findings suggest that Brassica oleracea peroxidase has the potential to efficiently remediate dye pollutants and dye-based industrial effluents in a green technology theme.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Review
Chemistry, Medicinal
Jose Guadalupe Osorio-Reyes, Hiram Martin Valenzuela-Amaro, Jose Juan Pablo Pizana-Aranda, Diana Ramirez-Gamboa, Edgar Ricardo Melendez-Sanchez, Miguel E. Lopez-Arellanes, Ma. Dolores Castaneda-Antonio, Karina G. Coronado-Apodaca, Rafael Gomes Araujo, Juan Eduardo Sosa-Hernandez, Elda M. Melchor-Martinez, Hafiz M. N. Iqbal, Roberto Parra-Saldivar, Manuel Martinez-Ruiz
Summary: Due to population growth and anthropological activity, there is a need for sustainable and affordable technologies to meet the current and future demand for agricultural products. The use of chemical fertilizers has caused nutrient shortages and environmental and health problems globally. Green biotechnology based on microalgae biomass offers a sustainable alternative for soil improvement and phytoremediation.
Review
Chemistry, Analytical
Lizeth Parra-Arroyo, Manuel Martinez-Ruiz, Sofia Lucero, Mariel A. Oyervides-Munoz, Mollie Wilkinson, Elda M. Melchor-Martinez, Rafael G. Araujo, Karina G. Coronado-Apodaca, Hugo Velasco Bedran, German Buitron, Adalberto Noyola, Damia Barcelo, Hafiz M. N. Iqbal, Juan Eduardo Sosa-Hernandez, Roberto Parra-Saldivar
Summary: Wastewater-Based Epidemiology (WBE) is a powerful tool for pandemic surveillance, but the reliability of wastewater results is affected by numerous variables. More comprehensive models are needed to study viral RNA degradation in complex matrices, and wastewater indicators identified through analytical chemistry can be used as health indicators and to determine the initial viral RNA content.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Muhammad Bilal, Ehsan Ullah Rashid, Junaid Munawar, Hafiz M. N. Iqbal, Jiandong Cui, Jakub Zdarta, Syed Salman Ashraf, Teofil Jesionowski
Summary: Nanobiocatalysts are an emerging field of nanobiocatalysis that integrates enzyme molecules into multifunctional materials like metal-organic frameworks (MOFs). Among these materials, functionalized magnetic MOFs have gained significant interest as versatile nano-biocatalytic systems for various organic transformations. These systems offer controllable enzyme microenvironments and show promise in enzyme engineering and biocatalytic transformations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Muhammad Bilal, Jakub Zdarta, Teofil Jesionowski, Hafiz M. N. Iqbal
Summary: Manganese peroxidases (MnPs) are enzymes with robust catalytic features that have gained attention in biotechnological applications, particularly in environmental remediation. They can degrade various organic pollutants and immobilized MnP fractions perform well in hostile environments. The article covers the microbial sources, structural attributes, immobilization strategies, and various biotechnological applications of MnP.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Sadia Anjum, Shakira Aslam, Nazim Hussain, Muhammad Bilal, Grzegorz Boczkaj, Wojciech Smulek, Teofil Jesionowski, Hafiz M. N. Iqbal
Summary: Given the current issues with global warming and rising greenhouse gas emissions, bio-hydrogen is a viable alternative fuel option. This study examines the different technologies and processes involved in bio-hydrogen production, as well as the limitations and challenges. Organic waste, particularly biodegradable waste, is the preferred feedstock for bio-hydrogen generation. The review also explores alternative options for improving process efficiency and discusses the hindrances to fuel-cell commercialization.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Editorial Material
Microbiology
Pooja Sharma, Surendra Pratap Singh, Hafiz M. N. Iqbal, Yen Wah Tong
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Jakub Zdarta, Filip Ciesielczyk, Muhammad Bilal, Katarzyna Jankowska, Karolina Bachosz, Oliwia Degorska, Agnieszka Rybarczyk, Teofil Jesionowski, Anne S. Meyer
Summary: Synthetic hormones are dangerous pollutants that pose risks to the reproductive health of both ecosystems and humans through water, including drinking water. Traditional wastewater treatment methods are not efficient enough. In this study, a new biosystem made of CaSiO3 and laccase was designed and fabricated, and its application for the removal of the synthetic estrogen 17 alpha-ethynylestradiol (EE2) from aqueous systems was demonstrated. The biosystem achieved 100% removal efficiency and showed synergistic degradation through simultaneous adsorption and biocatalytic conversion, with enzymatic conversion dominating.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Chemical
Peikun Li, Jiahui Jia, Zixin Geng, Saizhao Pang, Ruirui Wang, Muhammad Bilal, Hongjie Bian, Jiandong Cui, Shiru Jia
Summary: The glutamate oxidase (GLOX) and horseradish peroxidase (HRP) hybrid nanoflowers (GLOX&HRP-HNFs) were successfully prepared for the detection of glutamic acid (Glu). The GLOX&HRP-HNFs exhibited higher storage stability and resistance to organic solvents than free GLOX and HRP. Additionally, it showed a good linear range and a low limit of detection for glutamate.
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)