期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 107, 期 -, 页码 70-77出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.08.144
关键词
Chlorella ellipsoidea; Immunomodulatory activity; Molecular weight; Polysaccharide; Structure-bioactivity
资金
- Korea-China Cooperation Program - the Ministry of Trade, Industry and Energy
- Gangneung-Wonju National University in Korea
We investigated the effects of the protein and sulfate content, as well as the molecular weight (M-w), of green alga Chlorella ellipsoidea polysaccharides on their immunomodulatory activity. The deproteinized (DP1-3), desulfated (DS1-3), and hydrolyzed (DH1-3) derivatives of C. ellipsoidea polysaccharides were prepared by enzymatic hydrolysis, desulfation, and acid hydrolysis, respectively, of differing durations, resulting in preparations containing various amounts of proteins (2.41%-8.97%), sulfates (1.36%-4.89%), and M-w (51.5-193.4 kDa). The DH1-3-induced production of nitric oxide(NO) by RAW264.7 cells, decreased as the M-w of DH1-3 decreased. In addition, the sulfate content and M-w of DS1-3 affected the release of NO. However, lower protein content did not affect DP1-3-induced NO release and cytokine mRNA expression in RAW264.7 cells. Based on a multiple regression analysis of the effects of protein content, sulfate content, and M-w, on NO release, we found that M-w was a key factor for the stimulation of RAW264.7 cells, as it affected cytokine production, and activation of the nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) pathways. Therefore, the M-w of C. ellipsoidea polysaccharides played an important role in their immunomodulatory activities. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据