Article
Biochemical Research Methods
Yakun Fang, Fan Liu, Yi Shi, Ting Yang, Chaojuan Liang, Yu Xin, Guiyang Shi, Liang Zhang
Summary: This study investigated the thermostable skeleton and mechanism of action of the lipase TrLipB from Thermomicrobium roseum. The results showed that substitutions at specific sites affected the thermostability and catalytic efficiency of the enzyme.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Physical
Evdokia Vassiliadi, Anastasios Aridas, Veronique Schmitt, Aristotelis Xenakis, Maria Zoumpanioti
Summary: This study explores the use of a hybrid blend of biopolymers as a matrix for lipase immobilization. The results show that a HPMC:CS ratio of 2:1 forms the most promising matrix, and isooctane is the most effective reaction solvent. The enzyme-containing film also exhibits remarkable reusability, making it potentially valuable for various applications.
MOLECULAR CATALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Enheng Zhu, Xia Xiang, Sidi Wan, Huabiao Miao, Nanyu Han, Zunxi Huang
Summary: Lipases are versatile biocatalysts widely used in various industries. This study demonstrates that engineering the residue R209 can enhance the thermal stability of Thermomyces lanuginosus lipase (TLL). Molecular dynamics simulation analysis reveals the underlying mechanism of R209 and the 250-loop in modulating the thermostability of TLL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Purna B. Chetri, Rohit Shukla, Javed M. Khan, Aditya K. Padhi, Timir Tripathi
Summary: This study investigated the conformational changes of Fasciola gigantica malate dehydrogenase (FgMDH) in response to urea-induced unfolding. The results showed the formation of a partially folded intermediate state in FgMDH at low urea concentrations, which destabilized at higher urea concentrations and temperatures. The loss of enzymatic activity occurred before significant structural changes, indicating the importance of subtle structural alterations in substrate or cofactor binding site for activity loss.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Ashwani Kumar, Mitchell D. Nothling, Heather M. Aitken, Zeyun Xiao, Matthew Lam, Craig A. Bell, Megan L. O'Mara, Luke A. Connal
Summary: Inspired by the chemical structure of chymotrypsin, an artificial catalytic triad with high catalytic efficiency for the transesterification reaction of vinyl trifluoroacetate and methanol was developed. The addition of a hydrophobic chain allowed the formation of amphiphilic catalysts, which formed clusters for reactions in organic solvents. Molecular dynamics simulations, dynamic light scattering, and H-1 NMR revealed complex H-bonding interactions between the catalytic head groups of the artificial catalytic triad, resembling native enzymes.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Thermodynamics
Nur Sulihatimarsyila Abd Wafti, Robiah Yunus, Harrison Lik Nang Lau, Thomas Shean Yaw Choong, Suraini Abd-Aziz
Summary: The application of enzymes in the organic synthesis of polyol esters for biolubricants is explored in this study. TMP ester was synthesized from HO-PME through enzymatic transesterification. This research demonstrates that only 3% enzyme dosage is needed to achieve the highest triester yield of 82% without purification. The enzymatically synthesized TMP ester exhibits excellent lubricant properties.
Review
Food Science & Technology
Eduardo Leonarski, Karina Cesca, Debora de Oliveira, Acacio A. F. Zielinski
Summary: Anthocyanins, naturally occurring bioactive compounds found in fruits, vegetables, and grains, have great potential for technological applications. Enzymatic acylation of anthocyanins can improve their stability, making them more resistant to light and temperature, and their liposolubility allows for their addition to products with lipid matrices.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Zehua Zhang, Mengfei Long, Nan Zheng, Xiang Lu, Cailin Zhu, Tolbert Osire, Xiaole Xia
Summary: In this study, the effects of internal cavities on the thermostability and catalytic activity of enzymes were explored using molecular dynamics simulations and site-directed mutations. The results showed that altering the volume and spatial distribution of cavities can enhance the stability and activity of enzymes. This study provides important insights into the impact of internal cavities on enzymatic properties.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Rong Lu, Yanrui Ding
Summary: This study investigated the importance of water-protein interactions for protein structure and stability by analyzing the conformational changes of proteins at different temperatures. Results showed that stabilizing flexible regions is crucial for maintaining protein structure, and residues forming stable hydrogen bonds with water can also form stable interactions with other residues.
Article
Biochemistry & Molecular Biology
Vadim Kotov, Georg Mlynek, Oliver Vesper, Marina Pletzer, Jiri Wald, Celso M. Teixeira-Duarte, Herve Celia, Maria Garcia-Alai, Stephan Nussberger, Susan K. Buchanan, Joao H. Morais-Cabral, Christian Loew, Kristina Djinovic-Carugo, Thomas C. Marlovits
Summary: Protein stability is crucial for structural and biochemical research, and the NanoDSF assay offers a miniaturized approach for assessing protein stability by measuring intrinsic tryptophan fluorescence. By simplifying protein unfolding description, this method can provide improved stability measures, such as the apparent Gibbs free energy of unfolding, for ranking results. The assay is compatible with diverse protein samples, making it a valuable tool for various applications including protein complex and membrane protein analysis.
Article
Chemistry, Medicinal
Yuele Lu, Rong Zhan, Beibei Song, Yaoyao Zhou, Linjiang Zhu, Hanchi Chen, Xiaolong Chen
Summary: In this study, a strain of lipase-produced Acinetobacter sp. zjutfet-1 was screened from soil samples, which exhibited high catalytic activity and stereoselectivity for racemic methyl 2-chlorobutanoate. The lipase efficiently resolved the racemic substrate and obtained (S)-methyl 2-chlorobutanoate, indicating its potential in the industrial production of levetiracetam.
Review
Biotechnology & Applied Microbiology
Qingzhuo Wang, Naief H. Al Makishah, Qi Li, Yanan Li, Wenzheng Liu, Xiaoman Sun, Zhiqiang Wen, Sheng Yang
Summary: Clostridia have the potential to be engineered for the production of short- and medium-chain volatile esters, as they can produce acyl-CoA, organic acids, and alcohols as precursors for ester production, while also utilizing waste materials to produce other alcohols and acids.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hideo Maruyama, Keito Fukuchi, Hideshi Seki
Summary: The aim of this study is to develop a simulation model for the fractionation of fatty acid methyl esters (FAME) using urea complexation. The different complexation behaviors of SFAME, MFAME, and PFAME were observed, and a fractionation model was proposed to explain the observed phenomena.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Qiuhan Lin, Mingmin Zhang, Linan Zhang, Mimi Zhu, Kaiyi Qin, Pengcheng Wang
Summary: The synthesis and characterization of the new energetic material 4-amino-3-hydrazino-5-methyl-1,2,4-triazole has been investigated in this study. Various techniques including DSC, IR, elemental analysis, and NMR spectroscopy were employed to fully characterize the material. The detonation properties of the synthesized compounds were evaluated, and compounds 7 and 8 exhibited exceptional detonation properties with high pressures and velocities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
M. Gomez, M. D. Murcia, E. Gomez, J. L. Gomez, A. M. Hidalgo, M. C. Montiel, J. M. Hernandez
Summary: A new methodology has been developed to calculate the kinetic parameters of enzymatic reactions, and it has been applied to esterification under solvent-free conditions. This methodology uses integral analysis to solve the reactor model and obtain an explicit expression for the reaction time as a function of substrate conversion. The kinetic parameters were determined by minimizing the sum of square differences between experimental and calculated values. The effectiveness of the parameter determination and reactor model were validated by comparing theoretical and experimental values.
REACTION CHEMISTRY & ENGINEERING
(2023)
Review
Medicine, Research & Experimental
Arbind Kumar, Aashish Sharma, Narendra Vijay Tirpude, Sharad Thakur, Sanjay Kumar
Summary: In late 2019, a highly infectious and life-threatening virus called COVID-19 was first reported in Wuhan, China, and quickly spread globally. It belongs to the coronavirus family and causes severe respiratory disease similar to SARS. The pandemic has led to increasing morbidity and mortality rates, especially among vulnerable populations. Proper screening and early interventions are necessary to control the transmission and reduce the risk of severe complications. Diagnostic assays include molecular detection of viral genetic material and serological tests for detecting antibodies. Currently, there are no effective prophylactics or therapeutics, but some drugs are showing promising antiviral activity and are being tested in clinical trials. This review provides an insight into the laboratory detection methods for COVID-19 and the clinical stage of recommended drugs/vaccines against the novel SARS-CoV-2 virus.
CURRENT MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Aashish Sharma, Arbind Kumar, Mudasir Rashid, Ramchandra Vijay Amnekar, Sanjay Gupta, Jagdeep Kaur
Summary: Rv0428c, a hypothetical gene product in Mycobacterium tuberculosis genome, has been identified as a phagosomal acetyl transferase involved in the survival of bacteria under stress conditions. Its expression is upregulated under acidic and nutritive stress conditions, and it shows GCN5 acetyl transferase activity and stability over a wide pH and temperature range.
Review
Immunology
Arbind Kumar, Aashish Sharma, Narendra Vijay Tirpude, Suresh Sharma, Yogendra S. Padwad, Sanjay Kumar
Summary: This article discusses the severity and risk factors of hypercytokinemia in COVID-19 patients, with a special emphasis on prospective immunomodulatory therapies. Timely administration of immunomodulatory drugs may help mitigate the severity of the disease, reduce hospital stay, and lower mortality rate.
INFLAMMOPHARMACOLOGY
(2022)
Article
Biology
Pradeep Kumar Anand, Arbind Kumar, Amrit Saini, Jagdeep Kaur
Summary: In this study, the effect of a mutation in the EthA protein on its function was examined through structural and functional analysis. The results showed that the mutation led to a less stable protein structure and reduced binding affinity towards the anti-tuberculosis drug ETH. In vitro experiments validated the computational findings. The mutant strain exhibited higher growth and survival in the presence of the ETH drug, indicating decreased sensitivity of Mycobacterium tuberculosis to ETH.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Stuti Arya, Parul Singh, Jashandeep Kaur, Arbind Kumar, Jagdeep Kaur
Summary: This study investigated the expression pattern of Mycobacterium tuberculosis hormone-sensitive lipase (mHSL) under different environmental conditions, revealing that different sets of mHSL genes are expressed under various stress conditions, suggesting their important role in intracellular survival of mycobacteria.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Pharmacology & Pharmacy
Arbind Kumar, Aashish Sharma, Narendra Vijay Tirpude, Yogendra Padwad, Vipin Hallan, Sanjay Kumar
Summary: The SARS-CoV-2 outbreak has presented numerous challenges to the global healthcare and socioeconomic systems. Plant-derived immune adjuvants can enhance vaccine effectiveness and stimulate the necessary immune response to combat COVID-19.
PHARMACOLOGICAL REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Rakesh Kumar, Arbind Kumar, Jagdeep Kaur
Summary: This study presents the application of protein engineering technologies in improving the physical and catalytic properties of enzymes. Protein engineering is commonly used to enhance crucial enzyme characteristics, and a semi-rational approach has emerged as the preferred technology. However, the adoption of an engineering strategy depends on the availability of resources and subject-matter knowledge.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2023)
Article
Plant Sciences
Aditi Bisht, Shyna Bhalla, Arbind Kumar, Jagdeep Kaur, Neera Garg
Summary: Arbuscular mycorrhizal fungi play a significant role in improving the growth and antioxidant capacity of pigeon pea plants under cadmium stress. Fungal supplementation enhances nutrient acquisition, reduces oxidative damage, and activates metal chelator genes to alleviate cadmium toxicity.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Microbiology
Parul Singh, Arbind Kumar, Ravindresh Chhabra, Kashmir Singh, Jagdeep Kaur
Summary: This study elucidated the role of MSMEG_5850 in the physiology of mycobacteria. Transcriptome analysis revealed differential expression of 148 genes in an MSMEG_5850 knockout strain. Electrophoretic mobility shift assay and size exclusion chromatography confirmed the binding of MSMEG_5850 to its motif as a monomer. MSMEG_5850 was upregulated under nutritional stress and promoted the survival of mycobacteria.
FUTURE MICROBIOLOGY
(2023)
Article
Infectious Diseases
Suresh Kumar, Sahdev Choudhary, Sharad Thakur, Arbind Kumar, Sanjay Kumar
Summary: This study detected the presence of the Omicron variant during the third wave in India. All tested patients were found to be infected with the Omicron variant during this wave.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Article
Biochemistry & Molecular Biology
Pradeep Kumar Anand, Gagandeep Kaur, Varinder Saini, Jasbinder Kaur, Jagdeep Kaur
Summary: The multigene PE/PPE family is found exclusively in mycobacterium species. Rv3539, identified as PPE63, contains a conserved PPE domain at the N-terminal and a PE-PPE domain at the C-terminal. The study shows that Rv3539 has esterase activity, with the PE-PPE domain demonstrating higher enzyme activity. Additionally, the PPE domain is important for the thermostability and structural integrity of Rv3539.
Article
Microbiology
Pradeep K. K. Anand, Jagdeep Kaur
Summary: The modulation of host immune response is important for the intracellular survival of Mycobacterium tuberculosis. The PPE63 protein was shown to enhance intracellular survival of M. smegmatis by altering cell wall integrity and modulating the host immune response.
CURRENT MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)