期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 108, 期 -, 页码 250-258出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.11.157
关键词
Au nanoparticle; Cyclic voltammetry; Cytochrome c; Electron transfer; Nanocomposite; NO biosensensor
Goldnanoparticle (AuNPs), chitosan (CS), cytochrome c (Cyt c) and Nafion were immobilized on the surface of a gold electrode to form a Nafion/Cyt c/CS-3-Mercaptopropionic acid (MPA)-AuNPs/cysteamine-MPA (SAMs)-Au electrode. The CS-MPA-AuNPs nanocomposite was characterized by UV-vis spectroscopy. Electrochemical behavior of modified electrode was analyzed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and chronoamperometry. Uv-vis results showed that CS-MPA-AuNPs nanocomposite could improve electron transfer between Cyt c and electrode surface and keep the catalytic activity of Cyt c. A pair of well defined and reversible redox peaks could be observed for the modified electrode in a 0.1 M phosphate buffer solution (PBS, pH 7.0). The anodic and cathodic peak potentials of Cyt c/CS-MPA-AuNPs/SAMs-Au electrode were at 0.006 V and -0.043 V (vs. Ag/AgCI), respectively. Particular electrocatalytic activity was shown by Cyt con CS-MPA-AuNPs layer for nitric oxide (NO) reduction. The relationship between peak current and NO concentration was linear in the range of 1 x 10(-7)-21.5 x 10(-7)M with a detection limit of 0.45 x 10(-7) M (S/N =3). The sensitivity was 0.199 mu A/mu M. The obtained results indicating that the newly developed biosensor was quite selective and stable which was used for accurate and exact detection of NO. (C) 2017 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据