Article
Biochemistry & Molecular Biology
Te Yu, Di Wu, Bin Liang, Jiaming Wang, Xinzhu Shang, Qiong Wu
Summary: Edible films of Auricularia auricula polysaccharide (AAP) were prepared and characterized for their optical, morphological, and mechanical properties. These films were evaluated for their barrier, bactericidal, and antioxidant properties in cold meat packaging. The results showed that films prepared from 40% AAP had the best mechanical properties, with smooth surfaces, good water barrier properties, and preserved the quality of cold meat. Auricularia auricula polysaccharide is therefore a promising composite membrane additive.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Chun Bian, Lanyang Ji, Hang Qu, Zhenyu Wang
Summary: This study revealed that the polysaccharide AAP-b2 isolated from Auricularia auricula fruiting bodies exhibited significant antithrombotic activity by inhibiting platelet aggregation and thrombus formation, as well as enhancing anticoagulant mechanisms.
Article
Chemistry, Multidisciplinary
Jing Yao, Jiangying Zeng, Huinan Tang, Yirui Cheng, Jingjing Tan, Tianyuan Li, Xiangyu Li, Jiyuan He, Yongjun Zhang
Summary: This study evaluated the potential of ultra-sound-assisted natural deep eutectic extraction (UAE-DES) for the preparation of Auricularia auricula polysaccharides (AAPs) and examined their antioxidant capacity. Optimal parameters were determined using deionized water as the solvent, resulting in a 25.07% ± 0.43% yield at 50°C, 70 mL/g liquid-to-material ratio, and 150 W ultrasonic power. The yield of AAPs was increased by 18.68% using DES (ethylene glycol-choline chloride, molar ratio of 2:1) containing 40% water. AAPs-UD, prepared with DES as solvents, showed significantly stronger ABTS+/OH•/DPPH• scavenging capacity compared to AAPs-UW, and prolonged the lifespan of C. elegans. Furthermore, AAPs-UD up-regulated the expression of antioxidant-related genes in insulin and IGF-1 signaling pathway, as well as skn-1 in the target of rapamycin pathway.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Food Science & Technology
SolJu Pak, Fang Chen, Lingjun Ma, Xiaosong Hu, Junfu Ji
Summary: The review focuses on the bioactive components in black fungi, such as polysaccharides, melanin, and phenolic compounds, and their health benefits including antioxidant, hypoglycemic, antitumor, and immunostimulation effects. The biological activity and physicochemical properties of black fungi enable their potential applications in drug delivery and food production.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nannan Liu, Mengyin Chen, Juanna Song, Yuanyuan Zhao, Pin Gong, Xuefeng Chen
Summary: The polysaccharides of Auricularia auricula (AAP) have been found to improve type 2 diabetes by altering the gut microbiota in mice. AAP intervention can lower blood glucose levels, improve glucose tolerance, and reduce oxidative stress injury. Furthermore, AAP can regulate the abundance of beneficial and pathogenic bacteria, influencing the structure of the gut microbiota.
Article
Biochemistry & Molecular Biology
Xiaoting Yu, Ruiying Wang, Bin Lai, Mingqian Tan
Summary: The study demonstrated that the fermentation broth of Auricularia auricula has protective effects on the liver and stomach of mice with acute alcoholism, reducing levels of ALT, AST, TC, and TG in serum. The analysis found characteristic structures of active substances in the fermentation broth.
Article
Biochemistry & Molecular Biology
Yingjun Zhou, Yuezhong Jia, Nuo Xu, Lihua Tang, Yaning Chang
Summary: Obesity is greatly influenced by gut microbiota disorders, and Auricularia auricula-judae polysaccharides (AAPs) have shown potential anti-obesity effects. This study revealed that AAPs improved overweight, insulin resistance, glucose and lipid metabolism disorders, and liver damage in obese mice. AAPs also restored gut microbiota balance, inhibited harmful bacteria growth, and promoted the proliferation of beneficial bacteria. Furthermore, AAPs regulated the TLR4/JNK signaling pathway and activated AKT and AMPK. These findings highlight the importance of gut microbiota in the anti-obesity effects of AAPs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yemei Dai, Yuan Ma, Xiaocui Liu, Ruyun Gao, Hongmei Min, Siyu Zhang, Siyu Hu
Summary: In this study, Auricularia auricula-judae polysaccharide-based nanoparticles were prepared using an anti-solvent precipitation method. Response surface methodology was used for optimization. The results showed that the prepared nanoparticles had small size, uniform morphology, good dispersion, high thermal stability, and enhanced antioxidant activity.
Article
Biochemistry & Molecular Biology
Xiu-Zhi Fan, Fen Yao, Chao -Min Yin, De -Fang Shi, Hong Gao
Summary: This study investigated the impact of different carbon sources on the production, structure, and activities of fungal polysaccharides produced by submerged culture of Auricularia auricula-judae. Results showed that glucose was the most effective carbon source for mycelial biomass and polysaccharide production. Glucose-derived polysaccharides exhibited the best antioxidant activities and protection against alloxan-damaged islet cells. These findings lay the foundation for utilizing liquid-fermented A. auricula-judae mycelia and polysaccharides in functional food production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Sariya Mapoung, Sonthaya Umsumarng, Warathit Semmarath, Punnida Arjsri, Pilaiporn Thippraphan, Supachai Yodkeeree, Pornngarm Limtrakul (Dejkriengkraikul)
Summary: Auricularia auricula-judae, a nutrient-rich mushroom used in traditional medicine, has been found to promote wound healing through a water-soluble polysaccharide-rich extract (AAP). AAP showed antioxidant activity, increased fibroblast and keratinocyte proliferation, migration, and collagen synthesis while decreasing E-cadherin expression. In a mice skin wound-healing model, AAP significantly accelerated wound closure on days 9 and 12. Overall, AAP's wound healing activities can potentially be applied in wound management products.
Article
Chemistry, Applied
Min Lei, Mengqing Wang, Wentao Ma, Na Li, Jiwen Huang, Xinjian Jiang, Wenhui Wu, Chaoyan Zhang
Summary: A new polysaccharide AAP was extracted from Auricularia auricula, showing good antioxidant activity. The purified component pAAP exhibited a unique molecular structure and special conformation.
NATURAL PRODUCT RESEARCH
(2023)
Article
Food Science & Technology
Qian Liu, Ruisen Ma, Si Li, Yujie Fei, Jing Lei, Ruoyu Li, Yu Pan, Sining Liu, Langhong Wang
Summary: This study investigated the intervention effects and underlying molecular mechanisms of polysaccharides extracted from Auricularia auricula-judae resources in the Qinba Mountain area on nutritional obesity induced by high fat and high fructose diets (HFFD) in C57BL/6J mice. The results demonstrated that dietary supplementation of Auricularia auricula-judae polysaccharides significantly improved insulin resistance, altered serum lipid metabolites, and slowed down body weight gain induced by HFFD. The intervention effects were associated with the regulation of adipogenic-related gene expressions, cholesterol synthesis-related gene levels, fatty acid beta-oxidation-related gene expressions, and cholesterol efflux-related gene expressions in the liver, indicating improvements in hepatic lipid metabolism homeostasis. Furthermore, these effects were closely related to mitochondrial function.
Article
Biochemistry & Molecular Biology
Rui Zhou, Yijun Wang, Zaixu Wang, Ke Liu, Qi Wang, Honghui Bao
Summary: Auricularia auricula-judae polysaccharide (AP) has a significant impact on the properties of kidney bean starch, increasing its pasting viscosity, gel strength, and cold storage stability while reducing its digestibility. The combination of AP and KBST exhibits higher order and a firmer structure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Hui Dang, Qianqian Kong, William Winchester, Xin Wan, Yu Lei, Haisheng Zhang, Yu Zhao, Xinyu Liu, Ben Bin Xu, Baoshan Zhang, Zhe Wang
Summary: This study investigated the effect of Trichoderma fungi on the growth of Auricularia auricula in the main cultivation regions in China. It was found that Trichoderma pleuroticola had a strong competitive advantage over A. auricula and completely inhibited its mycelium growth.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Article
Chemistry, Applied
Jun Liang, Zi-Hao Rao, Si-Liang Jiang, Shu Wang, Hai-Xue Kuang, Yong -Gang Xia
Summary: A previously unknown glucuronoxylogalactoglucomannan (GXG ' G '' M), named ME-2, was isolated and purified from water extracts of Auricularia auricula-judae. ME-2 is a highly branched polysaccharide with an average of 10 branches per 10 sugar backbone units. ME-2 also exhibited anti-inflammatory activity. These findings contribute to the development and application of black woody ear polysaccharides as medicinal agents or functional dietary supplements.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)