期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 115, 期 -, 页码 176-184出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.03.110
关键词
Enzyme immobilization; Alginate lyase; Cross-linked enzyme aggregate; Alginate hydrogel; Enzyme-assisted degradation
资金
- NSF-ERC, Revolutionizing Metallic Biomaterials [EEC-0812348]
- NSF-CBET [0933153]
- Edward R. Weidlien Chair Professorship funds, University of Pittsburgh
- Center for Complex Engineered Multifunctional Materials (CCEMM), University of Pittsburgh
An enzyme aggregate of alginate lyase (EC 4.2.23) from flavobactierium was prepared using ammonium sulfate. The resultant aggregates upon cross-linking with glutaraldehyde produced insoluble and catalytically active cross-linked enzyme aggregate (CLEA) enzyme. The catalytic activity and stability of the cross-linked enzyme aggregate of alginate lyase (CLEA-AL) was studied in the presence of various pH, temperatures and organic solvents. Reusability, storage stability and surface morphology of the CLEA-AL were also studied. The native enzyme and CLEA-AL exhibited maximum enzyme activity at pH of 6.3 and at a temperature of 40 degrees C. The CLEA-AL has good stability in nonpolar organic solvents and is thermally stable up to 50 degrees C over a period of 8 h. By encapsulating CLEA-AL into alginate hydrogel, we demonstrate that alginate hydrogels can be enzymatically degraded in a controlled fashion. The results also showed that degradation of alginate hydrogel with CLEA-AL incorporated beads is slower than native enzyme and therefore, CLEA-AL can be used for controlled degradation and release of various biologics from the degrading gel. (C) 2018 Elsevier B.V. All rights reserved.
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