期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 113, 期 -, 页码 195-204出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.02.064
关键词
Polysaccharides; Polygonum multiflorum; Structure characterization; Immunomodulatory activity
资金
- Innovative Project on Designing and Screening Drug Candidates of Chongqing [cstc2015zdcy-ztzxl 20003]
- Key Project of Frontier and Basic Research of Chongqing Science and Technology Committee [cstc2015jcyjBX0010]
- Innovation Project Program of Chongqing Science and Technology Committee [cstc2015shmszx00014]
Two purified polysaccharides named WPMP-1 and WPMP-2 were obtained from Polygonum multiflorum with an average molecular weight of 2.04 kDa and 92.13 kDa, respectively. Structural characterization revealed that WPMP-1was supposed to be a glucan composed of 1,4-linked alpha-D-Glcp, 1,4,6-linked alpha-D-Glcp, and terminal alpha-D-Glcp. WPMP-2 contained a complex branched structre formed by 1,2-linked alpha-D-Rhap, 1,2,4-linked alpha-D-Rhap, 1,3,5-linked alpha-L-Ara, 1,5- linked alpha-L-Araf, terminal alpha-L-Araf, 1,4-linked beta-D-Galp, 1,4-linked alpha-D-GalpA, and 4-linked alpha-D-GalpA. In vitro, WPMPs were of activation effect on splenocyte and macrophages, and could also protect immunocyte against 5-Fu induced immunosupression by restoring the proliferation rate, phagocytic index and cytokines secretion level. Moreover, the acid polysaccharide WPMP-2 exhibited better immunomodulatory activity than neutral polysaccharide WPMP-1, which indicated that the immunomodulatory activity of WPMPs were positively associated with higher content of uronic acid, percentage of rhamnose, arabinose and galactose, and branching degree. These findings could promote the polysaccharides from Polygonum multiflorum to be attractive functional food supplement and immunomodulatory adjuvant. (C) 2018 Elsevier B.V. All rights reserved.
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