期刊
GENE
卷 554, 期 2, 页码 215-223出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2014.10.050
关键词
Brassica oleracea; Cold and freezing stress; Expression; Microarray
资金
- Golden Seed Project (Center for Horticultural Seed Development) [213003-04-2-CG100, 213003-04-2-SB420]
- Ministry of Agriculture, Food and Rural Affairs (MAFRA)
- Ministry of Oceans and Fisheries (MOF)
- Rural Development Administration (RDA)
- Korea Forest Service (KFS)
Cold and freezing stress is a major environmental constraint to the production of Brassica crops. Enhancement of tolerance by exploiting cold and freezing tolerance related genes offers the most efficient approach to address this problem. Cold-induced transcriptional profiling is a promising approach to the identification of potential genes related to cold and freezing stress tolerance. In this study, 99 highly expressed genes were identified from a whole genome microarray dataset of Brassica rapa. Blast search analysis of the Brassica oleracea database revealed the corresponding homologous genes. To validate their expression, pre-selected cold tolerant and susceptible cabbage lines were analyzed. Out of 99 BoCRGs, 43 were differentially expressed in response to varying degrees of cold and freezing stress in the contrasting cabbage lines. Among the differentially expressed genes, 18 were highly up-regulated in the tolerant lines, which is consistent with their microarray expression. Additionally, 12 BoCRGs were expressed differentially after cold stress treatment in two contrasting cabbage lines, and B0CRG54, 56, 59, 62, 70, 72 and 99 were predicted to be involved in cold regulatory pathways. Taken together, the cold-responsive genes identified in this study provide additional direction for elucidating the regulatory network of low temperature stress tolerance and developing cold and freezing stress resistant Brassica crops. (C) 2014 Elsevier B.V. All rights reserved.
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