期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 23, 页码 13007-13013出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b03041
关键词
bacteria; capsule; polysaccharides; fimbriae; biofilm; nanomechanics
资金
- Melbourne Materials Institute
- Australian Government National Health and Medical Research Council (NHMRC) [628770]
- NHMRC [606788]
- Australian Research Council [DP130100957]
- NSFC [11105090]
- CIGIT for Startup Foundation for Advanced Talents
- Chongqing Science and Technology Commission [cstc2013yykfC00007, cstc2013jcyjC00001]
- Chinese Academy of Sciences for a Key Scientific Instrument and Equipment Development Project
Bacteria form biofilms to facilitate colonization of biotic and abiotic surfaces, and biofilm formation on indwelling medical devices is a common cause of hospital-acquired infection. Although it is well-recognized that the exopolysaccharide capsule is one of the key bacterial components for biofilm formation, the underlying biophysical mechanism is poorly understood. In the present study, nanomechanical measurements of wild type and specific mutants of the pathogen, Klebsiella pneumoniae, were performed in situ using atomic force microscopy (AFM). Theoretical modeling of the mechanical data and static microtiter plate biofilm assays show that the organization of the capsule can influence bacterial adhesion, and thereby biofilm formation. The capsular organization is affected by the presence of type 3 fimbriae. Understanding the biophysical mechanisms for the impact of the structural organization of the bacterial polysaccharide capsule on biofilm formation will aid the development of strategies to prevent biofilm formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据