Review
Immunology
Mahmoud Mabrok, Abdelazeem M. M. Algammal, Elayaraja Sivaramasamy, Helal F. F. Hetta, Banan Atwah, Saad Alghamdi, Aml Fawzy, Ruben Avendano-Herrera, Channarong Rodkhum
Summary: Tenacibaculosis is a disease caused by the marine bacterial pathogen Tenacibaculum maritimum, resulting in high mortalities in various marine fish species globally. Despite advances in understanding its traits and pathogenesis mechanisms, the pathogenesis of tenacibaculosis is still not fully understood, highlighting the urgent need for continued research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Ruben Avendano-Herrera, Anne Berit Olsen, Monica Saldarriaga-Cordoba, Duncan J. Colquhoun, Victor Reyes, Javier Rivera-Bohle, Eric Duchaud, Rute Irgang
Summary: This study confirms the presence of Tenacibaculum piscium in Chile and Coho salmon, expanding the geographical and species distribution of this bacterium. The pathogenicity of the Chilean isolates was found to be low in Atlantic salmon, with observed scale loss and fin damage in dead fish. The whole-genome analysis revealed various genes associated with iron acquisition, secretion systems, antibiotic resistance, and stress responses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Fisheries
Rute Irgang, Ruben Avendano-Herrera
Summary: This study analyzes the susceptibility patterns of Tenacibaculum dicentrarchi to tiamulin and suggests that tiamulin is a viable alternative to florfenicol for the treatment of tenacibaculosis in the Chilean salmon industry.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Monica Saldarriaga-Cordoba, Rute Irgang, Ruben Avendano-Herrera
Summary: Tenacibaculum dicentrarchi is an emerging pathogen that causes high economic losses in the salmon industry in Chile. Comparative analysis of the genome sequences of different strains provides insights into the virulence process of this bacterium.
JOURNAL OF FISH DISEASES
(2021)
Article
Infectious Diseases
Pierre Lopez, Sebastien Bridel, Denis Saulnier, Rarahu David, Beatriz Magarinos, Beatriz S. Torres, Jean Francois Bernardet, Eric Duchaud
Summary: This study identified the genomic loci involved in the O-antigen biosynthesis of Tenacibaculum maritimum, a devastating bacterial pathogen affecting marine fish. By combining conventional serotyping and genome-wide association study, a robust multiplex PCR-based serotyping scheme was developed, outperforming traditional serotyping. This method could be useful for population structure analysis, disease surveillance, vaccine formulation, and efficacy follow-up.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
M. Pilar Escribano, Miguel Balado, Alicia E. Toranzo, Manuel L. Lemos, Beatriz Magarinos
Summary: This study analyzed the prevalence of extracellular proteolytic and lipolytic activities related to virulence in Tenacibaculum maritimum strains. They found a great intra-specific heterogeneity in enzymatic capacity, particularly within serotype O4. It was also discovered that OMVs, specifically enriched in certain proteins, played a key role in virulence and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Karthiga Kumanan, Ulla von Ammon, Andrew Fidler, Jane E. Symonds, Seumas P. Walker, Jeremy Carson, Kate S. Hutson
Summary: The suitability of three diagnostic techniques in detecting Tenacibaculum maritimum in farmed Chinook salmon was evaluated. The selective bacterial culture technique showed the highest detection rate, followed by ddPCR assay and nonselective culture medium. Co-occurring Tenacibaculum soleae and Tenacibaculum dicentrarchi were also isolated from the salmon. The study highlights the importance of using selective culture technique in combination with molecular techniques for rapid identification and management of emerging fish-pathogenic species.
Article
Fisheries
Monica Saldarriaga-Cordoba, Henry Araya-Leon, Rute Irgang, Ruben Avendano-Herrera
Summary: This study developed a novel PCR assay for the specific detection of Tenacibaculum piscium, an emerging disease that severely affects salmonid farming in Chile. The PCR method showed high sensitivity for T. piscium in seeded fish samples, but lower sensitivity for DNA extracted from mucus samples. Compared to other existing methodologies, the PCR approach allowed for the easiest detection of T. piscium in mucus samples obtained from challenged fish.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Macarena Echeverria-Bugueno, Rute Irgang, Jorge Mancilla-Schulz, Ruben Avendano-Herrera
Summary: Despite the presence of immune substances in the mucus layer of the fish skin, it is insufficient to resist Tenacibaculum dicentrarchi, leading to Tenacibaculosis in Atlantic salmon. Therefore, greater attention should be given to fish skin mucus as a primary defense mechanism against T. dicentrarchi.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Ines A. Ferreira, Diogo Peixoto, Ana Paula Losada, Maria Isabel Quiroga, Ana do Vale, Benjamin Costas
Summary: This study aimed to understand the early innate immune response of European sea bass to T. maritimum infection. The results showed that T. maritimum induced a local immune response in the skin, gills, and posterior-intestine of the fish. This may be due to the bacteria's ability to adhere, colonize, and damage these organs. The infected fish also exhibited systemic responses, including increased neutrophils, signs of anemia, and decreased bactericidal and lysozyme activities in the plasma.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Joseph P. Nowlan, John S. Lumsden, Spencer Russell
Summary: Quantitative PCR assays were developed for detecting Tenacibaculum species, showing low detection limits and suitable amplification efficiencies, but further investigation into other genes is needed. These assays may help elucidate the pathogenesis of tenacibaculosis and aid management practices in the aquaculture industry.
JOURNAL OF FISH DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Rohit Satyam, Shaban Ahmad, Khalid Raza
Summary: This study uses a comparative genomics approach to investigate the characteristic features of 26 publicly available genomes of Tenacibaculum and proposes reclassification and naming suggestions. The study found the co-occurrence of several antimicrobial resistance/virulence genes and genes private to specific members. It also explores non-B DNA forming regions, operons, tandem repeats, high-confidence putative effector proteins, and sortase that may play important roles in bacterial evolution, transcription, and pathogenesis.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Fisheries
Joseph P. Nowlan, Brianna M. Heese, Matthew J. Wilson, Scott R. Britney, John S. Lumsden, Spencer Russell
Summary: A qPCR assay targeting T. ovolyticum was developed and used to screen samples collected before, during, and after a mouthrot outbreak in BC Atlantic salmon. The results showed limited involvement of T. ovolyticum in this particular outbreak, highlighting the unclear ecological role and pathogenicity of T. ovolyticum in mouthrot outbreaks in Atlantic salmon.
Article
Fisheries
Sara Valdes, Rute Irgang, Maria C. Barros, Pedro Ilardi, Monica Saldarriaga-Cordoba, Javier Rivera-Bohle, Enrique Madrid, Johana Gajardo-Cordova, Ruben Avendano-Herrera
Summary: The study reported the first isolation of Tenacibaculum maritimum in rainbow trout farmed in Chile, confirming its pathogenic potential in Atlantic salmon and expanding the known host distribution. The research highlighted the need for further studies to evaluate the risk of T. maritimum for the O. mykiss farming industry.
JOURNAL OF FISH DISEASES
(2021)
Article
Microbiology
Joseph P. Nowlan, Scott R. Britney, John S. Lumsden, Spencer Russell
Summary: There is limited understanding of the pathogenesis of tenacibaculosis in Atlantic salmon and few reproducible exposure models for comparison. Different species of Tenacibaculum were used to expose Atlantic salmon, showing varied mortality rates and clinical signs. This experimental infection model will be helpful for further research on the pathogenesis, treatment, and immunity of tenacibaculosis.
Article
Environmental Sciences
Simonetta Corsolini, America Metzdorff, Davide Baroni, Jose L. Roscales, Begona Jimenez, Elena Cerro-Galvez, Jordi Dachs, Cristobal Galban-Malagon, Ondrej Audy, Jiri Kohoutek, Petra Pribylova, Matias Poblete-Morales, Ruben Avendano-Herrera, Elisa Bergami, Karla Pozo
Summary: The low air humidity in Antarctica necessitates the use of flame retardants in research facilities to prevent fires. Different types and concentrations of flame retardants were found in dust samples from various research stations and rooms.
ENVIRONMENTAL RESEARCH
(2021)
Article
Ecology
Franziska A. Stressmann, Joaquin Bernal-Bayard, David Perez-Pascual, Bianca Audrain, Olaya Rendueles, Valerie Briolat, Sebastian Bruchmann, Stevenn Volant, Amine Ghozlane, Susanne Haeussler, Eric Duchaud, Jean-Pierre Levraud, Jean-Marc Ghigo
Summary: This study identified a small consortium of culturable bacterial species that can protect zebrafish, as well as specific endogenous species and a bacterial assembly that contribute to community-level resistance against infection. The research demonstrates the importance of understanding the role of key endogenous bacteria in protecting hosts and the potential for engineering microbial communities for enhanced resilience against pathogens through direct experimental analysis.
Article
Fisheries
Rute Irgang, Ruben Avendano-Herrera
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Maritza Grandon, Rute Irgang, Jose Saavedra, Marcos Mancilla, Ruben Avendano-Herrera
Summary: Surveillance of antibiotic resistance in fish pathogens is crucial, particularly for Renibacterium salmoninarum in salmon farming. A study in southern Chile revealed an evolving subpopulation of non-wild-type isolates for erythromycin, while isolates were most susceptible to florfenicol and oxytetracycline. This information can be useful for antibiotic surveillance programs and future revisions in MIC values determination methods.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Hector A. Levipan, Ruben Avendano-Herrera
Summary: This study investigated the growth and cytotoxic effect of P. salmonis after exposure to salmonid skin mucus. The results showed that while skin mucus did not significantly affect bacterial growth, it delayed the cytotoxic response of the bacteria towards cells.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Macarena Echeverria-Bugueno, Cristobal Balada, Rute Irgang, Ruben Avendano-Herrera
Summary: The study found that Renibacterium salmoninarum is able to produce extracellular vesicles (EVs), which play a crucial role in host invasion and exhibit toxic effects. This information lays the foundation for potential applications of R. salmoninarum EVs in salmon aquaculture, inspiring further research and development against bacterial kidney disease.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Ruben Avendano-Herrera, Pablo Saldivia, Jorn Bethke, Cristian Vargas, Mauricio Hernandez
Summary: The study revealed that under iron-limited conditions, the H-2 strain of R. salmoninarum demonstrated better adaptation and had a significantly enriched iron ion homeostasis pathway and key virulence factors. Iron deficiency favored the expression of the 57-kDa protein in strain H-2, the principal virulence factor for R. salmoninarum.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Maria F. Morales-Rivera, Diego Valenzuela-Miranda, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Ruben Avendano-Herrera, Cristian Gallardo-Escarate
Summary: This study aimed to characterize the bacterial community associated with Caligus rogercresseyi in Chilean salmon aquaculture. It found that sea lice can act as potential reservoirs for fish diseases in the aquatic environment.
Editorial Material
Immunology
Hector A. Levipan, Luis Reyes-Garcia, Ruben Avendano-Herrera
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Melanie Hennart, Julien Guglielmini, Sebastien Bridel, Martin C. J. Maiden, Keith A. Jolley, Alexis Criscuolo, Sylvain Brisse
Summary: Current strategies for defining sublineages (SLs) within microbial species are lacking, causing confusion in population biology and epidemiological surveillance. In this study, a genomic classification and nomenclature approach for bacterial strains was proposed using Klebsiella pneumoniae as a model. A dual barcoding system combining multilevel single linkage clustering and life identification numbers (LINs) was devised to define SLs based on a core genome multilocus sequence typing scheme. The proposed taxonomy provides stability and nomenclatural continuity with previous nomenclature, and is accessible through a community-curated platform.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Infectious Diseases
Ruben Avendano-Herrera, Anne Berit Olsen, Monica Saldarriaga-Cordoba, Duncan J. Colquhoun, Victor Reyes, Javier Rivera-Bohle, Eric Duchaud, Rute Irgang
Summary: This study confirms the presence of Tenacibaculum piscium in Chile and Coho salmon, expanding the geographical and species distribution of this bacterium. The pathogenicity of the Chilean isolates was found to be low in Atlantic salmon, with observed scale loss and fin damage in dead fish. The whole-genome analysis revealed various genes associated with iron acquisition, secretion systems, antibiotic resistance, and stress responses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Pierre Lopez, Sebastien Bridel, Denis Saulnier, Rarahu David, Beatriz Magarinos, Beatriz S. Torres, Jean Francois Bernardet, Eric Duchaud
Summary: This study identified the genomic loci involved in the O-antigen biosynthesis of Tenacibaculum maritimum, a devastating bacterial pathogen affecting marine fish. By combining conventional serotyping and genome-wide association study, a robust multiplex PCR-based serotyping scheme was developed, outperforming traditional serotyping. This method could be useful for population structure analysis, disease surveillance, vaccine formulation, and efficacy follow-up.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Multidisciplinary Sciences
Sebastien Bridel, Valerie Bouchez, Bryan Brancotte, Sofia Hauck, Nathalie Armatys, Annie Landier, Estelle Muhle, Sophie Guillot, Julie Toubiana, Martin C. J. Maiden, Keith A. Jolley, Sylvain Brisse
Summary: This study presents a large database of Bordetella isolates and genomes, along with the development of genotyping systems for the whole genus and for the B. pertussis Glade. It provides a valuable resource for evolutionary and epidemiological research on whooping cough and other Bordetella infections.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Yueying Zhu, Delphine Lechardeur, Jean-Francois Bernardet, Brigitte Kerouault, Cyprien Guerin, Dimitri Rigaudeau, Pierre Nicolas, Eric Duchaud, Tatiana Rochat
Summary: Bacterial pathogens have a significant impact on aquaculture, with the Flavobacterium psychrophilum being responsible for bacterial cold-water disease in salmonids worldwide. This study identified two new TonB-dependent receptors, HfpR and BfpR, that play essential roles in adaptation to iron conditions during infection and in virulence in rainbow trout.
Article
Ecology
Cyprien Guerin, Bo-Hyung Lee, Benjamin Fradet, Erwin van Dijk, Bogdan Mirauta, Claude Thermes, Jean-Francois Bernardet, Francis Repoila, Eric Duchaud, Pierre Nicolas, Tatiana Rochat
Summary: This study conducted extensive transcriptome analysis of Flavobacterium psychrophilum, identifying genome maps, transcription start sites, and gene expression profiles across various conditions. It highlighted the connection between genes, environmental conditions, and phenotypic traits, providing insights into gene regulation and revealing characteristics linked to the phylogenetic position and ecological niche of the bacterium. The results also suggested potential directions for future research on protein and regulatory elements involved in host-pathogen interactions, metabolic pathways, and molecular machineries.
ISME COMMUNICATIONS
(2021)