Article
Developmental Biology
Maria Ohno, Masataka Nikaido, Natsumi Horiuchi, Koichi Kawakami, Kohei Hatta
Summary: The enteric nervous system in zebrafish larvae at 10-15 days postfertilization shows high regenerative ability, with enteric neurons recovering to about 50% of the control after laser ablation. Live imaging revealed neurites covering the unablated area and entry of ENCDCs into the ablated areas within 1-3 days. This process involves proliferation, entrance, and neurogenesis of ENCDCs, providing a basis for further in vivo research in this vertebrate model.
Article
Multidisciplinary Sciences
Brenda Minerva Farias-Serratos, Ivan Lazcano, Patricia Villalobos, Veerle M. Darras, Aurea Orozco
Summary: In this study, reduced availability of T3 resulted in decreased myelin density and oligodendrocyte precursor cell numbers in zebrafish embryos. Treatment with T3 rescued the effects, indicating the crucial role of thyroid hormones in regulating myelination during early brain development.
Article
Developmental Biology
Timothy J. Duerr, Eun Kyung Jeon, Kaylee M. Wells, Antonio Villanueva, Ashley W. Seifert, Catherine D. McCusker, James R. Monaghan
Summary: In this study, a transgenic line of axolotls expressing FUCCI was generated to investigate cell division and cell contribution in regeneration. The results showed widespread expression of FUCCI in developing and adult tissues, which was validated by DNA synthesis and mitosis phase markers. Spinal cord amputation was found to increase cell proliferation at a certain distance from the injury site, and multimodal staining provided cell type information for cycling cells.
Article
Multidisciplinary Sciences
Stefania Ippati, Yuanyuan Deng, Julia van der Hoven, Celine Heu, Annika van Hummel, Sook Wern Chua, Esmeralda Paric, Gabriella Chan, Astrid Feiten, Thomas Fath, Yazi D. Ke, Nikolas K. Haass, Lars M. Ittner
Summary: The study suggests that neurons may exhibit transient, self-limited cell cycle reentry activity, indicating a dynamic process in their postmitotic state; neurons undergoing cell cycle reentry activity during oligomeric amyloid-beta challenge can maintain and increase the cell cycle indicator signal, thus evading cell death.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ravi K. Gupta, Petra Mlcochova
Summary: SARS-CoV-2 infection triggers redistribution of cyclin D1 and D3 from the nucleus to the cytoplasm, followed by proteasomal degradation. Cyclin D3 interacts with SARS-CoV-2 proteins and affects the assembly and release of newly produced virions, highlighting the importance of understanding the virus-host interactions.
Article
Pathology
Andrea Ronchi, Federica Zito Marino, Giorgio Toni, Francesca Pagliuca, Daniela Russo, Giuseppe Signoriello, Elvira Moscarella, Gabriella Brancaccio, Giuseppe Argenziano, Renato Franco, Immacolata Cozzolino
Summary: This study aimed to evaluate the performance of melanocytic markers when applied to cell blocks obtained from fine needle aspiration cytology of lymph node metastases from cutaneous melanoma. Results showed that SOX10 had the best staining performance with a 100% detection rate, making it strongly recommended to be included in the minimal immunocytochemical panel for diagnostic evaluation in suspected CM metastasis patients.
JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jennifer Alisa Amrhein, Lena Marie Berger, Amelie Tjaden, Andreas Kraemer, Lewis Elson, Tuomas Tolvanen, Daniel Martinez-Molina, Astrid Kaiser, Manfred Schubert-Zsilavecz, Susanne Mueller, Stefan Knapp, Thomas Hanke
Summary: The PCTAIRE subfamily of CDKs, including CDK16, represents an understudied class of kinases with potential implications in various diseases. By optimizing the structure, a highly potent inhibitor 43d was identified, which selectively targets CDK16 and other members of the PCTAIRE and PFTAIRE families. Cellular assays and cell cycle analysis confirmed the inhibitory effect of 43d.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuki Hitora, Ai Sejiyama, Koyo Honda, Yuji Ise, Fitje Losung, Remy E. P. Mangindaan, Sachiko Tsukamoto
Summary: Natural products play important roles in drug development, specifically in cancer biology and the development of antitumor agents. In this study, a new sesquiterpene quinone, neoisosmenospongine, was isolated from a marine sponge extract, along with four known compounds. These compounds were found to inhibit cell cycle progression at S/G2/M phases, demonstrating the effectiveness of fluorescent image-based high-content screening for isolating cell cycle inhibitors from natural resources.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jake W. Willows, Gilian Gunsch, Emma Paradie, Magdalena Blaszkiewicz, Jeffrey R. Tonniges, Maria F. Pino, Steven R. Smith, Lauren M. Sparks, Kristy L. Townsend
Summary: Peripheral neuropathy, including axonal degeneration and demyelination, affects adipose tissues in obesity, diabetes, and aging. This study investigated the presence of demyelinating neuropathy in adipose tissue and found that adipose tissue contains myelinated and unmyelinated nerves populated by Schwann cells (SCs), which play a role in nerve regeneration. The study also showed that SCs in adipose tissue become dysregulated in diabetic peripheral neuropathy.
Article
Neurosciences
He Wang, Mengjia Liu, Zhuoyang Ye, Cuihua Zhou, Huiru Bi, Long Wang, Chen Zhang, Hui Fu, Ying Shen, Jian-Jun Yang, Yimin Hu, Guiquan Chen
Summary: This study reveals that the phosphorylation of FoxO1 by Akt plays a critical role in Sox10 expression and OL differentiation. Deletion of all three Akt isoforms leads to downregulation of Sox10, and mutant FoxO1 inhibits Sox10 promoter activity.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Dermatology
Anja Wessely, Elias A. T. Koch, Julio Vera, Carola Berking, Markus V. Heppt
Summary: Uveal melanoma, the most common eye tumor in adults, lacks effective treatment options. Researchers propose using immune checkpoint blockade and identifying novel therapeutic targets to improve treatment outcomes.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Biology
Alexander P. Browning, Jesse A. Sharp, Ryan J. Murphy, Gency Gunasingh, Brodie Lawson, Kevin Burrage, Nikolas K. Haass, Matthew Simpson
Summary: Tumour spheroids are common experimental models in vitro, closely mimicking avascular tumour growth. Research suggests that spheroids have a limiting structure and the mature structure is independent of seeding density. Additionally, comparing spheroid structure rather than size produces more accurate results.
Article
Immunology
Gelei Xiao, Kaiyue Wang, Zeyu Wang, Ziyu Dai, Xisong Liang, Weijie Ye, Peng Luo, Jian Zhang, Zaoqu Liu, Quan Cheng, Renjun Peng
Summary: Using machine learning, this study found that the SOX family has significant immune characteristics and genomic profiles in gliomas. It can be divided into two distinct clusters, with SOX10 identified as an excellent immune regulator of macrophage in gliomas. High expression of SOX10 is associated with shorter overall survival but benefits from immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Yu Teng, Ping Hu, Yangyang Chen, Yanyu Zang, Xiaolian Ye, Jingmin Ou, Guiquan Chen, Yun Stone Shi
Summary: Leucine-rich glioma-inactivated protein 1 (LGI1) plays a key role in autosomal dominant lateral temporal lobe epilepsy (ADLTE). A novel mutation on the Lgi1 gene was identified, which may cause white matter abnormalities by inhibiting Sox10-dependent oligodendrocyte differentiation and myelination in the central nervous system.
Article
Genetics & Heredity
Lijuan Huang, Maosheng Guo, Ningdong Li
Summary: This study describes the clinical characteristics of three sporadic patients with Waardenburg syndrome and congenital exotropia and investigates the disease-causing genes for them. The results suggest that Waardenburg syndrome may be involved in malfunction in multiple nerve systems.
FRONTIERS IN GENETICS
(2022)
Article
Developmental Biology
Rosa A. Uribe, Stephanie S. Hong, Marianne E. Bronner
DEVELOPMENTAL BIOLOGY
(2018)
Article
Developmental Biology
Neha Ghosh, Asif Bakshi, Risha Khandelwal, Sriivatsan Govinda Rajan, Rohit Joshi
Article
Multidisciplinary Sciences
Phillip A. Baker, Matthew D. Meyer, Ashley Tsang, Rosa A. Uribe
SCIENTIFIC REPORTS
(2019)
Article
Gastroenterology & Hepatology
Alanna R. Condren, Maria S. Costa, Natalia Rivera Sanchez, Sindhu Konkapaka, Kristin L. Gallik, Ankur Saxena, Brian T. Murphy, Laura M. Sanchez
Article
Biology
Aubrey G. A. Howard, Phillip A. Baker, Rodrigo Ibarra-Garcia-Padilla, Joshua A. Moore, Lucia J. Rivas, James J. Tallman, Eileen W. Singleton, Jessa L. Westheimer, Julia A. Corteguera, Rosa A. Uribe
Summary: Utilizing single-cell transcriptomic analyses, this study delineated NCC-derivatives in the zebrafish, identifying major cell types and over three dozen cellular subtypes. Gene expression signatures of pigment progenitors, mesenchyme cells, and enteric NCCs were dissected, revealing combinatorial hox gene codes demarcating NCC derivatives, with distinct profiles in neuronal cells. The comprehensive cell-type atlas presented in this study can serve as a valuable resource for further investigations into NCC differentiation mechanisms and evolution.
Article
Cell Biology
Randall W. Treffy, Sriivatsan G. Rajan, Xinghang Jiang, Lynne M. Nacke, Usama A. Malkana, L. A. Naiche, Dani E. Bergey, Dianicha Santana, Vinodh Rajagopalan, Jan K. Kitajewski, John P. O'Bryan, Ankur Saxena
Summary: This study demonstrates that the differentiation and survival of human NB cells change significantly when migrating with neural crest cells. The research shows that cells differentiate into neurons when migrating to the head, but remain undifferentiated when migrating posteriorly.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Aubrey G. A. Howard, Aaron C. Nguyen, Joshua Tworig, Priya Ravisankar, Eileen W. Singleton, Can Li, Grayson Kotzur, Joshua S. Waxman, Rosa A. Uribe
Summary: Neural crest cells (NCCs) are migratory and multipotent stem cells essential to vertebrate embryonic development. This study identifies the gene Hoxb5b as a regulator of NCC development, demonstrating that elevated Hoxb5b levels promote expansion of zebrafish NCCs and specifically expand expression domains of vagal NCC markers. The study also shows that early increase in vagal NCCs leads to supernumerary enteric neural progenitors, but these fail to properly expand into enterically fated neurons in the gut tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Aubrey G. A. Howard, Rosa A. Uribe
Summary: This review discusses the process of neural crest epithelial-to-mesenchymal transition (EMT) and the roles of extracellular matrix (ECM) and cellular effector proteins in the migration of neural crest cells. It also introduces the regulation of EMT and ECM remodeling during neural crest cell ontogenesis by Hox transcription factors.
Article
Biochemistry & Molecular Biology
Kuan-Lin Wu, Joshua A. Moore, Mitchell D. Miller, Yuda Chen, Catherine Lee, Weijun Xu, Zane Peng, Qinghui Duan, George N. Phillips, Rosa A. Uribe, Han Xiao
Summary: Genetic code expansion technology allows the creation of semisynthetic organisms using noncanonical amino acids. However, high concentrations of exogenously fed ncAAs are required due to inefficient cellular uptake and incorporation, especially in eukaryotic cells. To overcome this, researchers have engineered a metabolic pathway for the synthesis of O-methyltyrosine (OMeY) that allows cells to autonomously biosynthesize and incorporate OMeY into proteins at higher levels than exogenously fed cells. This technology has been successfully extended to mammalian cells and zebrafish model, providing valuable models for biological and biomedical research.
Article
Biochemical Research Methods
Beatriz A. Ibarra, Xinghang Jiang, Randall W. Treffy, Ankur Saxena
Summary: This article describes a method for xenotransplanting human neuroblastoma cells into zebrafish embryos, allowing for high-resolution imaging and tracking of cancer cell interactions with cells and the environment.
Article
Developmental Biology
Sriivatsan G. Rajan, Lynne M. Nacke, Jagjot S. Dhingra, Ankur Saxena
Summary: This study in zebrafish identified the earliest time window of olfactory MCC differentiation, showing that OMCC cells derive from peridermal cells and are regulated by the Notch signaling pathway. The research also found regionally segregated Notch signaling regulates the number of OMCCs and the differentiation-associated genes during olfactory development.
CELLS & DEVELOPMENT
(2021)