Article
Immunology
Yanni Zhang, Kang Zhu, Hui Lv, Jingguo Chen, Chao Yu, Tianxi Gao, Jing Yan, Fangli Yang, Lina Fan, Xiaoyong Ren, Cui Xia
Summary: This study found that serum exosome-derived miR-146a-3p may serve as a novel biomarker for predicting the short and long-term efficacies of sublingual immunotherapy (SLIT) in allergic rhinitis (AR) patients.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Marta Barbosa, Catia Gomes, Catarina Sequeira, Joana Goncalves-Ribeiro, Carolina Campos Pina, Luis A. Carvalho, Rui Moreira, Sandra H. Vaz, Ana Rita Vaz, Dora Brites
Summary: This study investigated the role of miR-146a in ALS astrocytes and found that upregulating miR-146a can alleviate the detrimental effects of astrocytes on neurons and microglia, suggesting miR-146a as a potential therapeutic target for ALS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Dianquan Zhang, Guoliang Cai, Kai Liu, Zhe Zhuang, Kunping Jia, Siying Pei, Xiuzhen Wang, Hong Wang, Shengnan Xu, Cheng Cui, Manchao Sun, Sihui Guo, Wenli Song, Guofeng Cai
Summary: This study found that M2 microglial exosomes (BV2-Exo) were internalized by neurons, attenuating neuronal apoptosis in response to ischemic injury and reducing infarct volume and behavioral deficits in ischemic mice. Exosomal miRNA-137 played a role in this neuroprotective effect by targeting Notch1.
Article
Cell Biology
Lu Liu, Manli Chen, Kun Lin, Xuwu Xiang, Jing Yang, Yueying Zheng, Xiaoxing Xiong, Shengmei Zhu
Summary: TRPC6 channel plays a crucial role in reducing inflammatory responses and apoptosis in astrocytes during ischemia/reperfusion injury, offering a potential target for stroke therapy. Activation of TRPC6 with HYP9 alleviates brain damage, while SKF exacerbates injury in ischemic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Veronica Granatiero, Nicole M. Sayles, Angela M. Savino, Csaba Konrad, Michael G. Kharas, Hibiki Kawamata, Giovanni Manfredi
Summary: ALS is a complex disease involving motor neuron degeneration, with emerging evidence suggesting the importance of astrocytes and the MTOR pathway in disease pathogenesis. Modulation of the astrocytic IGF1R-MTOR pathway may present a potential therapeutic strategy for SOD1 ALS and other neurological disorders.
Article
Biochemistry & Molecular Biology
Rosa Curcio, Giulia Poli, Consuelo Fabi, Chiara Sugoni, Maria Bruna Pasticci, Roberto Ferranti, Monica Rossi, Ilenia Folletti, Leandro Sanesi, Edoardo Santoni, Irene Dominioni, Massimiliano Cavallo, Giovanni Morgana, Lorenzo Mordeglia, Giovanni Luca, Giacomo Pucci, Stefano Brancorsini, Gaetano Vaudo
Summary: We investigated the association between circulating microRNAs and fibrosis development in COVID-19-related ARDS survivors. miR-17-5p, miR-223-3p, and miR-146a-5p were significantly down-regulated in patients with fibrosis-like lesions. Our results support the hypothesis that NLRP3-Inflammasome could be implicated in the process of fibrotic evolution of COVID-19-associated ARDS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tereza Filipi, Zuzana Matusova, Pavel Abaffy, Ondrej Vanatko, Jana Tureckova, Sarka Benesova, Monika Kubiskova, Denisa Kirdajova, Jakub Zahumensky, Lukas Valihrach, Miroslava Anderova
Summary: This study comprehensively investigates the disease-related activity of glia in the cortex of SOD1(G93A) mice using single-cell RNA sequencing and immunohistochemistry techniques. The results show minimal changes in glia throughout disease progression and regardless of sex. However, there were subtle disease-related transcriptional alterations in microglia and oligodendrocytes at the end-stage, supported by immunohistochemistry. Therefore, it is recommended to use a different model for future studies of the cortical ALS pathology.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Zhenhua Qiu, Zhihui Zhong, Yuehan Zhang, Haoling Tan, Bo Deng, Guohuang Meng
Summary: HucMSC-derived exosomal miR-335-5p attenuates the inflammation and EMT of HK-2 cells by reducing ADAM19 protein levels upon TGF-beta 1 induction. This study provides a potential therapeutic strategy and identifies targets for clinically treating renal fibrosis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Shatakshi Shukla, Fatema Currim, Rajesh Singh
Summary: Exosomes play an important role in intercellular communication and maintaining cellular homeostasis during development and differentiation. Dysregulation in exosome-mediated communication can lead to developmental defects and chronic diseases. Exosomes are heterogeneous and can vary in size, membrane protein abundance, and cargo content. This review focuses on the latest developments in exosome biogenesis pathways, heterogeneity, selective cargo enrichment, and isolation techniques. Understanding the heterogeneity and selective cargo enrichment of extracellular vesicles may provide insights into disease severity and early prognosis, and specific subtypes of exosomes can be used as therapeutic tools and biomarkers for specific diseases.
BIOLOGY OF THE CELL
(2023)
Article
Neurosciences
Bo Lei, Jiaxin Liu, Zhihui Yao, Yan Xiao, Xiaoling Zhang, Yueting Zhang, Jianguo Xu
Summary: The study revealed that NF-kappa B-induced upregulation of miR-146a-5p promoted oxidative stress and pyroptosis in AD-HNNs by targeting TIGAR. Knockdown of NF-kappa B or overexpression of TIGAR significantly attenuated oxidative stress and pyroptosis in AD-HHNs, while concurrent overexpression of miR-146a-5p inhibited these effects.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Review
Pharmacology & Pharmacy
Kenta Takanashi, Keisuke Shibata, Keita Mizuno, Ryohei Komatsu, Schuichi Koizumi
Summary: Platinum derivatives and taxanes like paclitaxel often induce chemotherapy-induced peripheral neuropathy, but the Japanese KAMPO medicine Goshajinkigan may inhibit this by acting on astrocytes. Research shows that astrocytes in the S1 cortex play a significant role in the pathogenesis of paclitaxel-induced neuropathy and could be a potential therapeutic target.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2021)
Article
Biotechnology & Applied Microbiology
Wenjing Gong, Yang Guo, Hang Yuan, Rui Chai, Ziang Wan, Boan Zheng, Xinye Hu, Bingchen Chen, Shan Gao, Qiaoqiong Dai, Peng Yu, Shiliang Tu
Summary: Hypoxia and exosome-mediated transfer of miRNAs from cancer-associated fibroblasts (CAFs) promote colorectal cancer (CRC) progression.
CANCER GENE THERAPY
(2023)
Article
Cell Biology
Catia Gomes, Catarina Sequeira, Shibi Likhite, Cassandra N. Dennys, Stephen J. Kolb, Pamela J. Shaw, Ana R. Vaz, Brian K. Kaspar, Kathrin Meyer, Dora Brites
Summary: Transdifferentiation of fibroblasts from ALS patients into astrocytes can help study the pathophysiology of the disease. Modulating miR-146a can restore neuroprotective phenotype and improve neuronal function in ALS patients.
Article
Biochemistry & Molecular Biology
Irina Gilyazova, Elizaveta Ivanova, Valentin Pavlov, Guzel Khasanova, Aliya Khasanova, Adel Izmailov, Dilara Asadullina, Gulshat Gilyazova, Guoqing Wang, Ilgiz Gareev, Ozal Beylerli, Elza Khusnutdinova
Summary: The study analyzed the expression levels of miRNA-146a, miRNA-126, miRNA-218, miRNA-410, miRNA-503, and miRNA-155 in HFRS patients at different stages and with different severity. The results showed that miRNA-155 and miRNA-146a may be potential prognostic biomarkers in HFRS. Further investigations are needed to evaluate the changes in miRNA expression and the network of genes that can be potential targets for the studied miRNAs in order to elucidate the molecular mechanisms that can influence the occurrence and development of HFRS.
NON-CODING RNA RESEARCH
(2023)
Article
Cell Biology
Adelaide Fernandes, Claudia Caldeira, Carolina Cunha, Elisabete Ferreiro, Ana Rita Vaz, Dora Brites
Summary: In this study, the temporal profile of inflammatory mediators and microglia deactivation/activation in the brain cortex and hippocampus of 3xTg-AD mice was examined. The results showed different regulation patterns in different age groups of mice, and potential biomarkers were identified. These findings provide new insights into the development of Alzheimer's disease and suggest critical targets for drug discovery and combinatorial therapies.
Review
Pharmacology & Pharmacy
Gecioni Loch-Neckel, Ana Teresa Matos, Ana Rita Vaz, Dora Brites
Summary: sEVs, as a drug delivery system, possess desirable features and can be used to treat neurodegeneration associated with CNS diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Goncalo Garcia, Adelaide Fernandes, Frank Stein, Dora Brites
Summary: The study reveals that miR-124 plays a significant role in the inflammation and pathophysiology of Alzheimer's disease. The modulation of miR-124 in neuroblastoma cells affects the polarization of microglia subtypes in the context of inflammation. Furthermore, the study demonstrates the transfer of miR-124 from neurons to microglia, highlighting the importance of neuronal survival in the underlying mechanisms of inflammation in Alzheimer's disease.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Catia Gomes, Catarina Sequeira, Shibi Likhite, Cassandra N. Dennys, Stephen J. Kolb, Pamela J. Shaw, Ana R. Vaz, Brian K. Kaspar, Kathrin Meyer, Dora Brites
Summary: Transdifferentiation of fibroblasts from ALS patients into astrocytes can help study the pathophysiology of the disease. Modulating miR-146a can restore neuroprotective phenotype and improve neuronal function in ALS patients.
Review
Biochemistry & Molecular Biology
Fabiola de Carvalho Chaves de Siqueira Mendes, Marina Negrao Frota de Almeida, Manoela Falsoni, Marcia Lorena Ferreira Andrade, Andre Pinheiro Gurgel Felicio, Luisa Taynah Vasconcelos Barbosa da Paixao, Fabio Leite do Amaral Junior, Daniel Clive Anthony, Dora Brites, Cristovam Wanderley Picanco Diniz, Marcia Consentino Kronka Sosthenes
Summary: This article reviews the impact of mastication and environmental enrichment on the structure of the brain and highlights the increased complexity of astrocytes in situations where neuronal loss and behavioral deficits are present.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pediatrics
Ermelinda Santos Silva, Susana Rocha, Rita Candeias Ramos, Helena Coutinho, Cristina Catarino, Fernanda Teixeira, Graca Henriques, Ana Isabel Lopes, Alice Santos-Silva, Dora Brites
Summary: This study found a potential association between bile acids profile and redox status in healthy infants, with the type of diet potentially influencing this relationship.
PEDIATRIC RESEARCH
(2023)
Editorial Material
Pharmacology & Pharmacy
Ana Rita Vaz, Ana Sofia Falcao, Valle Palomo
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Goncalo Garcia, Sara Pinto, Sofia Ferreira, Daniela Lopes, Maria Joao Serrador, Adelaide Fernandes, Ana Rita Vaz, Alexandre de Mendonca, Frank Edenhofer, Tarja Malm, Jari Koistinaho, Dora Brites
Summary: MiR-21 is found to be overexpressed in glial cells and exosomes in Alzheimer's disease (AD), and also shows high expression in the cerebrospinal fluid (CSF) with potential discriminatory ability. MiR-21 elevation is observed in microglia, neurons, and astrocytes in AD patients, and may be associated with the repression of its target, PPAR alpha.
Article
Biochemistry & Molecular Biology
Marta Barbosa, Marta Santos, Nidia de Sousa, Sara Duarte-Silva, Ana Rita Vaz, Antonio J. Salgado, Dora Brites
Summary: The intrathecal administration of the secretome from anti-miR-124-treated mSOD1 MNs is a potential therapeutic strategy for halting/delaying disease progression in an ALS mouse model. It prevents motor impairment, muscle atrophy, glial reactivity/dysfunction, and neurodegeneration, while regulating gene expression and inflammatory miRNAs.
Review
Neurosciences
Rosa C. Paolicelli, Amanda Sierra, Beth Stevens, Marie-Eve Tremblay, Adriano Aguzzi, Bahareh Ajami, Ido Amit, Etienne Audinat, Ingo Bechmann, Mariko Bennett, Frederick Bennett, Alain Bessis, Knut Biber, Staci Bilbo, Mathew Blurton-Jones, Erik Boddeke, Dora Brites, Bert Brone, Guy C. Brown, Oleg Butovsky, Monica J. Carson, Bernardo Castellano, Marco Colonna, Sally A. Cowley, Colm Cunningham, Dimitrios Davalos, Philip L. De Jager, Bart de Strooper, Adam Denes, Bart J. L. Eggen, Ukpong Eyo, Elena Galea, Sonia Garel, Florent Ginhoux, Christopher K. Glass, Ozgun Gokce, Diego Gomez-Nicola, Berta Gonzalez, Siamon Gordon, Manuel B. Graeber, Andrew D. Greenhalgh, Pierre Gressens, Melanie Greter, David H. Gutmann, Christian Haass, Michael T. Heneka, Frank L. Heppner, Soyon Hong, David A. Hume, Steffen Jung, Helmut Kettenmann, Jonathan Kipnis, Ryuta Koyama, Greg Lemke, Marina Lynch, Ania Majewska, Marzia Malcangio, Tarja Malm, Renzo Mancuso, Takahiro Masuda, Michela Matteoli, Barry W. McColl, Veronique E. Miron, Anna Victoria Molofsky, Michelle Monje, Eva Mracsko, Agnes Nadjar, Jonas J. Neher, Urte Neniskyte, Harald Neumann, Mami Noda, Bo Peng, Francesca Peri, V. Hugh Perry, Phillip G. Popovich, Clare Pridans, Josef Priller, Marco Prinz, Davide Ragozzino, Richard M. Ransohoff, Michael W. Salter, Anne Schaefer, Dorothy P. Schafer, Michal Schwartz, Mikael Simons, Cody J. Smith, Wolfgang J. Streit, Tuan Leng Tay, Li-Huei Tsai, Alexei Verkhratsky, Rommy von Bernhardi, Hiroaki Wake, Valerie Wittamer, Susanne A. Wolf, Long-Jun Wu, Tony Wyss-Coray
Summary: Microglial research has made significant progress, but the current classification system fails to accurately describe their diversity, leading to misconceptions about their functions. To address this issue, a group of multidisciplinary experts has proposed a naming framework and recommendations to help researchers better understand and describe the different states and functions of microglia.
Article
Clinical Neurology
Catarina Barros, Andreia Barateiro, Alexandre Neto, Beatriz Soromenho, Afonso P. Bastos, Joana M. Mateus, Sara Xapelli, Ana M. Sebastiao, Dora Brites, Luis Graca, Adelaide Fernandes
Summary: Studies have shown a correlation between excessive S100B and demyelination in multiple sclerosis. This research highlights the potential of using S100B-neutralizing small-molecule pentamidine as a therapeutic target to reduce damage and promote recovery in chronic experimental autoimmune encephalomyelitis.
BRAIN COMMUNICATIONS
(2022)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
