期刊
FOOD CONTROL
卷 84, 期 -, 页码 536-543出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2017.08.036
关键词
Lateral flow immunoassay; Gold nanoparticle; Signal amplification; Salmonella Enteritidis; Detection
资金
- National Natural Science Foundation of China [21675127, 31501560]
- New Century Excellent Talents in University [NCET-13-0483]
- Fundamental Research Funds for the Northwest A&F University of China [2014YB093, 2452015257]
A signal amplified lateral flow immunoassay (LFIA) based on gold nanoparticles (GNPs) growth and accumulation for rapid detection of Salmonella Enteritidis (S. Enteritidis) was developed. The signal was amplified on account of the high catalytic activity of GNPs toward the reaction between HAuCl4 and NH2OH . HCI, which could produce new GNPs on the surface of the initial GNPs. The remarkable enhanced signal at the test and control lines can be clearly distinguished by naked eyes even under a lower analyte level. The lowest visible detection limit of the signal enhanced LFIA for S. Enteritidis was as low as 10(4) CFU/mL with excellent specificity, achieving a 100-fold improvement in sensitivity compared to the traditional GNP-based LFIA. Finally, for applicability demonstration the method was applied to milk for S. Enteritidis analysis. Except for incubation time, the process required only 6 h, which was obvious shorter than that of many reported methods. (C) 2017 Elsevier Ltd. All rights reserved.
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