期刊
FOOD CHEMISTRY
卷 242, 期 -, 页码 62-67出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.09.040
关键词
Sea buckthorn; Hippophae; ITS2; Bar-HRM
资金
- Major Scientific and Technological Special Project for Significant New Drug Creation [2014ZX09304307]
- National Natural Science Foundation of China [81473428, 81703809]
- Study on the Key Safety Validation Elements of CM in International Trade [2015DFM30030]
- Foundation for Basic Research Program of China [2014FY130400]
- Development of Medicinal and Edible Plant Resources Key Laboratory of Universities in Sichuan Province [10Y201707]
Sea buckthorn (Hippophae), an ancient crop with modern virtues, is increasingly consumed in source of foods and nutraceuticals. The growing demand leads to the adulteration of commercial sea buckthorn products, which is a common form of food fraud. Herein, a high resolution melting assay, targeting a DNA barcoding region of the internal transcribed spacer 2 (ITS2) (Bar-HRM) was developed to identify the seven native Chinese Hippophae species, and to authenticate commercial sea buckthorn products. Melting data from the HRM assay demonstrated that all Hippophae species could be clearly distinguished. Then, application to commercial sea buckthorn products indicated the existence of adulterants or contamination, further confirmed using Sanger sequencing results for PCR products from HRM. The Bar-HRM technique proposed in this work could provide a method for regulatory agencies, promoting consumers trust, and raise the quality and safety of sea buckthorn products.
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