Article
Chemistry, Applied
Yahui Guo, Panting Sang, Gang Lu, Xue Yang, Yunfei Xie, Zhigang Hu, He Qian, Weirong Yao
Summary: An artificial deoxyribozyme was used as a substitute for horseradish peroxidase (or alkaline phosphatase) in ELISA for generating amplified signals. The feasibility of the proposed deoxyribozyme-based ELISA (DLISA) was demonstrated in the detection of a forbidden veterinary drug, chloramphenicol. With a detection limit of 0.1 ng/L and a wide linear range from 0.000001 μg/mL to 1.0 μg/mL, DLISA showed excellent practical performances. The easy connection of antibody and DNAzyme through gold nanoparticles in DLISA made it worth popularizing.
Article
Chemistry, Analytical
Rentang Huang, Yuxin Huang, Hongmei Liu, Kaiyi Guan, Ali Chen, Xiangsheng Zhao, Shumei Wang, Lei Zhang
Summary: In this study, a bifunctional gold nanoparticles probe-based enzyme-linked immunosorbent assay (nano-ELISA) was developed for detecting zearalenone in food. The method showed high sensitivity and accuracy, and could overcome matrix interference. It also had good correlation with liquid chromatography-tandem mass spectrometry results.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Wanhe Wang, Xueliang Wang, Jingqi Liu, Chuankai Lin, Jianhua Liu, Jing Wang
Summary: This article introduces the combination of PCR and gold nanoparticles (AuNPs) to construct colorimetric sensing platforms for the visual detection of health-related DNA and proteins. Future trends and perspectives are also discussed.
Article
Chemistry, Analytical
Shireen Zangana, Tomas Lednicky, Attila Bonyar
Summary: This paper presents the fabrication technology of surface nanocomposites based on hexagonally ordered gold nanoparticles (AuNPs) and their potential application as surface-enhanced Raman spectroscopy (SERS) substrates. Three different hexagonal arrangements were prepared on a large surface area using a nanotextured template and solid-state dewetting. The densely packed nanoparticle arrangements with tunable interparticle gaps showed high hot-spot density, making them beneficial for SERS applications. The substrate-based enhancement factor of the nanocomposites ranged from 4 x 10^4 to 2 x 10^6 for the different particle arrangements, and the sensing characteristics of a small dye molecule were also determined.
Article
Chemistry, Analytical
Hemeiling Cai, Jing Sun, Zhichang Ma, Junwei Di
Summary: An enhanced charge transfer between BiVO4 and Au nanoparticles was utilized to develop a turn-on photoelectrochemical biosensor for sensitive detection of target DNA. The biosensor showed a linear range from 1 aM to 10 pM with a detection limit of 0.2 aM, making it a promising platform for ultrasensitive DNA detection.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Nguyen Hoang Minh, Jae Sung Yoon, Do Hyun Kang, Yeong-Eun Yoo, Kwanoh Kim
Summary: Nanogap biosensors are promising platforms for detecting biochemical substances at low concentrations, but their fabrication processes and equipment can be arduous and costly. This research presents a novel design and fabrication process of vertical nanogap structures that can detect and quantify low-concentration biochemical substances. The nanogaps are created by magnetically assembling antibody-coated nanowires onto a nano-disk patterned between microelectrodes. The outcome of this research could inspire the design and fabrication of nanogap devices and nanobiosensors, with broad impacts on microfluidics, biochips, and lab-on-a-chip architectures.
Article
Biotechnology & Applied Microbiology
Dikshita Panwar, Deepali Shrivastava, Arvind Kumar, Lavleen Kumar Gupta, N. S. Sampath Kumar, Anjani Devi Chintagunta
Summary: In this study, a quick and effective strategy for isolating exosomes from serum was investigated using antibodies coupled with gold nanoparticles. This approach could be useful for clinically monitoring the biological state of patients.
Article
Chemistry, Analytical
Li Wang, Zhi-Jun Liu, Hai-Xia Cao, Guo-Xi Liang
Summary: A simple colorimetric assay using unmodified AuNPs and magnetic 3D DNA walker was developed to detect miRNA-155 at a sensitivity of 16.7 fM, solving the low sensitivity issue of AuNPs-based colorimetric nucleic acid assays. The platform activated DNA walker through DNAzyme cleavage mechanism, generating displaced ssDNA for visualization and quantification of miRNA-155 concentration by color change and UV-vis spectroscopy. This approach showed potential for POC nucleic acids diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Yao Shi, Jie Wu, Wenxin Wu, Nini Luo, Hao Huang, Yuhui Chen, Jun Sun, Qian Yu, Hang Ao, Qiqi Xu, Xiaotian Wu, Qianfeng Xia, Huangxian Ju
Summary: Electrocheluminescence analysis using coreaction accelerators has achieved breakthroughs in ultrasensitive biomarker detection. The proposed strategy utilizes AuNPs@MoSe2 heterostructure to enhance the ECL efficiency of the Ru(bpy)(2+)(3)/TPrA system. This coreaction accelerator successfully enhances ECL intensity by accelerating the generation of TPrA(circle+) radicals. The developed immunosensor based on this strategy demonstrates excellent selectivity, stability, and reproducibility, making it promising for clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Environmental Sciences
Hessamaddin Sohrabi, Mir Reza Majidi, Karim Asadpour-Zeynali, Alireza Khataee, Mahsa Dastborhan, Ahad Mokhtarzadeh
Summary: A gene detection platform was developed to detect Shigella dysenteriae in human plasma samples, incorporating biochar, MoS2 nanosheets, and Au nanoparticles. The platform demonstrated high selectivity and sensitivity, with LOD and LOQ values calculated under optimal conditions. The method also showed good repeatability and could be easily reused for subsequent detection assays, highlighting its potential for practical applications in genetic screening.
Article
Chemistry, Multidisciplinary
Almudena Marti, Jurriaan Huskens
Summary: Affinity sensing of nucleic acids is a highly investigated area in biosensing, and in this study, a simple electrochemical DNA detection layer is developed using poly-l-lysine and gold nanoparticles as a signal amplifier. The method shows high contrast and sensitivity, making it potentially useful in DNA diagnostics.
Article
Chemistry, Multidisciplinary
Jing Yang, Yuan Liang, Xiang Li, Yongpeng Zhang, Long Qian, Yonggang Ke, Cheng Zhang
Summary: The programmable assembly of gold nanoparticle superstructures with precise spatial arrangement has attracted attention for their unique characteristics in plasmonics and biomedicine. This article presents a strategy of using a spatially programmable enzymatic nanorobot arm to modulate the anisotropic assembly of DNA/AuNP superstructures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Xin Wang, Xiaomei Mu, Jinshen Li, Guang Liu, Shulin Zhao, Jianniao Tian
Summary: The CRISPR-Cas system has potential as a nucleic acid signal amplification technology, but the efficiency of the trans-cleavage reaction is low. A new 3D DNA nanomachine is developed by immobilizing CRISPR-Cas12a on nanoparticles to enhance its trans-cleavage activity. This nanomachine enables sensitive detection of miRNA-21 with a detection limit of 8.0 pM, providing insights for future bioanalysis and clinical diagnoses.
ANALYTICA CHIMICA ACTA
(2023)
Article
Oncology
Jeong-Yub Kim, Won Seok Lee, Seung-Jun Seo, Chan-Woong Jung, Eun Ho Kim
Summary: This study evaluated the toxicity of gold nanoparticles (GNP, AuNPs) combined with radiation therapy on hepatocytes. The results showed that the combination treatment had no significant effect on cell survival, apoptosis, DNA doublestrand breaks, or cell cycle. Additionally, there was no significant reduction in hepatocyte viability, proliferation, or secretory capacity. Therefore, the combination of AuNPs and radiation may be an effective therapy for hepatocellular carcinoma (HCC) with minimal cytotoxicity on normal tissue.
TRANSLATIONAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Weiwei Zhang, Wenwen Wang, Yueyue Yu
Summary: A multi-amplified electro-chemiluminescence aptasensor was developed for efficient recognition of the tumor marker CA125 in ovarian cancer. The sensor utilizes a tetrahedral DNA nanostructure and gold nanoparticles/Ru(bpy)32+/metal-organic framework signal probe for enhanced detection. The aptasensor shows high sensitivity and reliability, making it suitable for clinical analysis of CA125 detection.
BIOELECTROCHEMISTRY
(2024)
Article
Agriculture, Multidisciplinary
Zexiang Wang, Song Hu, Huanhuan Bao, Keyu Xing, Jintao Liu, Jun Xia, Weihua Lai, Juan Peng
Summary: The study developed a fast, reliable, and sensitive immunochromatographic assay for the detection of SMZ in food, with good detection limits and linear ranges. Recovery experiments showed high average recoveries in egg, honey, and pork samples.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Review
Food Science & Technology
Yina Huang, Keyu Xing, Liang Qiu, Qinglong Wu, Hua Wei
Summary: Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder with unknown causes. Tea, with its active ingredients like tea polyphenols, has shown potential benefits for preventing and treating IBD due to its anti-inflammatory and antioxidant properties, opening up possibilities for further research on tea derivatives as novel therapeutic agents for IBD.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Medicine, Research & Experimental
Zhen Huang, Bo Ning, He S. Yang, Brady M. Youngquist, Alex Niu, Christopher J. Lyon, Brandon J. Beddingfield, Alyssa C. Fears, Chandler H. Monk, Amelie E. Murrell, Samantha J. Bilton, Joshua P. Linhuber, Elizabeth B. Norton, Monika L. Dietrich, Jim Yee, Weihua Lai, John W. Scott, Xiao-Ming Yin, Jay Rappaport, James E. Robinson, Nakhle S. Saba, Chad J. Roy, Kevin J. Zwezdaryk, Zhen Zhao, Tony Y. Hu
Summary: The study demonstrated that CRISPR-augmented RT-PCR can sensitively detect SARS-CoV-2 RNA in blood, improving the accuracy of COVID-19 diagnosis. This approach accurately identified COVID-19 patients with negative nasal swab RT-qPCR results, aiding in the assessment of infection clearance and prediction of infection severity.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Food Science & Technology
Shan Shan, Yanmei Huang, Zhaohong Huang, Zhonger Long, Chengwei Liu, Xuelong Zhao, Keyu Xing, Xiaoyue Xiao, Jintao Liu, Yunhong Huang, Weihua Lai, Daofeng Liu
Summary: In this study, a method for accurate and rapid subtyping of STEC single colonies was developed using asPCR combined with LFIA, allowing for identification and subtyping of STEC with different Shiga toxin gene types.
Article
Nanoscience & Nanotechnology
Bo Ning, Zhen Huang, Brady M. Youngquist, John W. Scott, Alex Niu, Christine M. Bojanowski, Kevin J. Zwezdaryk, Nakhle S. Saba, Jia Fan, Xiao-Ming Yin, Jing Cao, Christopher J. Lyon, Chen-zhong Li, Chad J. Roy, Tony Y. Hu
Summary: Measuring circulating SARS-CoV-2 RNA in plasma may offer a more accurate way to detect lower respiratory tract and extrapulmonary infections missed by traditional COVID-19 detection methods. The study demonstrates the use of a CRISPR-Cas-based strategy to detect SARS-CoV-2 RNA in plasma-circulating extracellular vesicles, showing promising characteristics for clinical applications. Plasma SARS-CoV-2 RNA could be a viable diagnostic alternative to respiratory RNA levels, especially as respiratory RNA rapidly declines after infection.
NATURE NANOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Keyu Xing, Juan Peng, Wenyao Chen, Bolong Fang, Daofeng Liu, Shan Shan, Ganggang Zhang, Yina Huang, Weihua Lai
Summary: A label-free, colorimetric, and fluorescence dual-channel sensing platform was developed for the detection of zearalenone in corn, allowing for quantitative detection as low as 0.89 µg kg-1 and visual detection as low as 2.5 µg kg-1. The formation of plasmonic gold nanoparticles aggregates based on charge neutralization by SYBR Green I eliminates instability in traditional gold nanoparticles colorimetric measurements, paving the way for accurate and label-free bioanalysis.
Article
Agriculture, Dairy & Animal Science
Bolong Fang, Juan Peng, Gan Zhang, Keyu Xing, Wenyao Chen, Daofeng Liu, Shan Shan, Yonghua Xiong, Weihua Lai
Summary: In this study, stable Ag+-doped gold nanoclusters (Ag-AuNC) were synthesized and used for detecting Escherichia coli O157:H7 in the field of food safety. The combination of I2 and I- was applied to quench the enhanced fluorescence of Ag-AuNC and improve the sensitivity of detection. The proposed immunoassay exhibited excellent sensitivity, specificity, recovery, and accuracy for quantitative detection of Escherichia coli O157:H7 in food safety.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Engineering, Environmental
Hong Hu, Jun Xia, Nengshui Ding, Yonghua Xiong, Keyu Xing, Bolong Fang, Xi Huo, Weihua Lai
Summary: A sensitive and direct detection method for amantadine was developed using Ag-Au nanorings as colorimetric nanosensors combined with ELISA. The proposed method showed higher sensitivity, lower detection limits, and a wider linear range compared to conventional ELISA, making it a promising tool for the qualitative and quantitative detection of amantadine.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Zexiang Wang, Keyu Xing, Nengshui Ding, Suhua Wang, Ganggang Zhang, Weihua Lai
Summary: A new LFIA method based on the dual spectral-overlapped fluorescence quenching of PDANs is proposed for sensitive detection of SMZ, showing higher fluorescence intensity changes and lower detection limits compared to AuNPs. This method is suitable for detecting hazardous materials in practical applications.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Immunology
Zhen Huang, Lili Zhang, Christopher J. Lyon, Bo Ning, Brady M. Youngquist, Alex Niu, Brandon J. Beddingfield, Nicholas J. Maness, Nakhle S. Saba, Chen-Zhong Li, Chad J. Roy, Tony Y. Hu
Summary: This study utilized CRISPR-RT-PCR assay to analyze dynamic changes in SARS-CoV-2 RNA level and distribution in various specimen types of non-human primates. The results showed that CRISPR-RT-PCR consistently detected SARS-CoV-2 RNA in all sample types, while traditional RT-qPCR had lower sensitivity. The study also observed differences in abundance and kinetics of SARS-CoV-2 RNA between pulmonary and extrapulmonary specimens.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Multidisciplinary Sciences
Ibolya Rutkai, Meredith G. Mayer, Linh M. Hellmers, Bo Ning, Zhen Huang, Christopher J. Monjure, Carol Coyne, Rachel Silvestri, Nadia Golden, Krystle Hensley, Kristin Chandler, Gabrielle Lehmicke, Gregory J. Bix, Nicholas J. Maness, Kasi Russell-Lodrigue, Tony Y. Hu, Chad J. Roy, Robert Blair, Rudolf Bohm, Lara A. Doyle-Meyers, Jay Rappaport, Tracy Fischer
Summary: COVID-19 infection can lead to neurological manifestations, and animal models can provide insights into the underlying mechanisms. In this study, neuroinflammation, microhemorrhages, and brain hypoxia were observed in SARS-CoV-2 infected non-human primates, even in those without severe respiratory disease. These findings are important for understanding the neuropathogenesis of SARS-CoV-2 infection and may help explain the neurological symptoms associated with long COVID.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
Zhen Huang, Sylvia M. LaCourse, Alexander W. Kay, Joshua Stern, Jaclyn N. Escudero, Brady M. Youngquist, Wenshu Zheng, Debrah Vambe, Muyalo Dlamini, Godwin Mtetwa, Lisa M. Cranmer, Irene Njuguna, Dalton C. Wamalwa, Elizabeth Maleche-Obimbo, Donald G. Catanzaro, Christopher J. Lyon, Grace John-Stewart, Andrew DiNardo, Anna M. Mandalakas, Bo Ning, Tony Y. Hu
Summary: This study found that using CRISPR to detect Mycobacterium tuberculosis cell-free DNA in blood has the potential to increase the diagnosis rate of pediatric tuberculosis and HIV-associated tuberculosis, as well as to be used for early diagnosis and rapid monitoring of tuberculosis treatment responses.
Article
Agriculture, Dairy & Animal Science
K. Y. Xing, H. H. Bao, N. S. Ding, Y. H. Xiong, J. Peng, W. H. Lai
Summary: A colorimetric and surface-enhanced Raman scattering (SERS) signal amplification platform based on 2-step aggregation of gold nanoparticles (AuNP) was developed for the sensitive detection of melamine. The study found that the use of positively charged SYBR Green I and melamine could induce the aggregation of AuNP, resulting in significant color and SERS signal changes. The sensitivity of the 2-step aggregation-based platform was higher than the 1-step aggregation-based method for melamine detection.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Analytical
Zhou Xu, Qingxia Li, Yixuan Wang, Shaoyi Yu, Ye Jiao, Li Wen, Yanna Jiao, Jia Tu, Keyu Xing, Yunhui Cheng
Summary: A dual-mode immunosensor based on upconversion fluorescence (UCFL) and magnetic relaxation switch (MRS) was developed for the anti-interference and sensitive detection of cadmium ion (Cd2+). The UCFL mode solved the interference of pigment fluorescence in pellucid or colored samples, while the MRS mode overcame the disturbance of turbid and opaque samples. The Cd2+ could be detected by UCFL and MRS dual-mode immunosensor with limits of detection of 0.038 ng/mL and 0.16 ng/mL, respectively.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Food Science & Technology
Keyu Xing, Huanhuan Bao, Nengshui Ding, Yonghua Xiong, Juan Peng, Weihua Lai
Summary: A novel charge neutralization strategy using positively charged SYBR Green I was employed for the convenient detection of fumonisin B1. The presence of fumonisin B1 can neutralize the positive charge of SYBR Green I, preventing the aggregation of gold nanoparticles. Two methods, colorimetry and surface enhanced Raman spectroscopy (SERS), were developed based on this charge neutralization strategy for the detection of fumonisin B1 in corn. The colorimetric method had a naked eye sensitivity of 500 μg/kg, while the SERS method had a limit of detection of 54.86 μg/kg. Both methods exhibited high stability with a coefficient of variation < 8.3%.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
