期刊
FOOD CHEMISTRY
卷 257, 期 -, 页码 182-188出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.02.139
关键词
Antioxidants; Animal tissues; UHPLC; UPLC; DAD; Fluorescence
资金
- EFS
- Spanish Government [INIA RZP2013-00001-00]
- Aragon Government [A49]
An accurate, fast, economic and simple method to determine carotenoids, tocopherols, retinol and cholesterol in lyophilised samples of ovine milk, muscle and liver and raw samples of fat, which are difficult to lyophilise, is sought. Those analytes have been studied in animal tissues to trace forage feeding and unhealthy contents. The sample treatment consisted of mild overnight saponification, liquid-liquid extraction, evaporation with vacuum evaporator and redissolution. The quantification of the different analytes was performed by the use of ultra-high performance liquid chromatography with diode-array detector for carotenoids, retinol and cholesterol and fluorescence detector for tocopherols. The retention times of the analytes were short and the resolution between analytes was very high. The limits of detection and quantification were very low. This method is suitable for all the matrices and analytes and could be adapted to other animal species with minor changes.
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