期刊
FOOD CHEMISTRY
卷 242, 期 -, 页码 45-52出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.09.017
关键词
Lactobacillus brevis; Exopolysaccharides; alpha-D-Glucan; NMR analysis
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [114O695]
- Biotechnology and Biological Science Research Council (BBSRC) Strategic Core Grant [BB/J004529/1]
- BBSRC [BBS/E/F/000PR10356, BBS/E/F/00044453] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/F/000PR10356, BBS/E/F/00044453] Funding Source: researchfish
Cereal-associated Lactic Acid Bacteria (LAB) are well known for homopolymeric exopolysaccharide (EPS) production. Herein, the structure of an EPS isolated from sourdough isolate Lactobacillus brevis E25 was determined. A modified BHI medium was used for production of EPS-E25 in order to eliminate potential contaminants. Analysis of sugar monomers in EPS revealed that glucose was the only sugar present. Structural characterisation of EPS by NMR and methylation analysis revealed that E25 produced a highly branched a-glucan with (alpha 1 -> 3) and (alpha 1 -> 6) glycosidic linkages, and was similar in structure to a previously reported EPS from Lactobacillus reuteri 180. The H-1 and C-13 NMR data were contrasted with newly recorded data for known polysaccharides (alternan, commercial dextran) which also contain alpha-(1,3,6) Glc branch points. It was found in both E25 EPS and alternan that NMR parameters could be used to distinguish glucose residues that had the same substitution pattern but occupied different positions in the structure.
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