Isocoumarin derivatives and monoterpene glycoside from the seeds of Orychophragmus violaceus

Five new compounds, including four new isocoumarin derivatives [orychophramarin A-D (1-4)] and a new monoterpene glycoside [orychovioside A (5)], together with fourteen known compounds were isolated from the seeds of Orychophragmus violaceus. Their structures were established on the basis of chemical evidence and spectroscopic analysis. Compound 1 showed significant cytotoxicity against HCT-116 and Hela cell lines compared with the positive control group (Cisplatin) with IC50 values of 5.10 and 8.91 mu M, respectively. Compound 1 exhibited evident cell cycle retardation that arrested HCT-116 cells at G(2) phase and induced apoptosis in HCT-116 cells in the further mechanism study.