Article
Biochemistry & Molecular Biology
Lijie Liu, Cuiying Chen, Yewei Dong, Yu Cheng, Cuihong You, Shuqi Wang, Hongyu Ma, Yuanyou Li
Summary: This study showed that insulin could activate the biosynthesis of LC-PUFA in rabbitfish hepatocytes through the PI3K/Akt/mTOR/Srebp1 pathway, indicating a potentially important regulatory mechanism for lipid metabolism in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Yewei Dong, Lijie Liu, Mengmeng Li, Dizhi Xie, Jianhong Zhao, Shuqi Wang, Cuihong You, Yuanyou Li
Summary: The study found that the rabbitfish is the first marine teleost with the ability to synthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C-18 precursors through the regulation of insulin response elements (IREs) with the involvement of transcription factors Srebp-1c and Sp1.
Article
Biochemistry & Molecular Biology
Fanny Janet De la Cruz-Alvarado, Carlos A. Alvarez-Gonzalez, Raul Llera-Herrera, Oscar Monroig, Naoki Kabeya, Sergio Rodriguez-Morales, Bartolo Concha-Frias, Rocio Guerrero-Zarate, Luis D. Jimenez-Martinez, Emyr Saul Pena-Marin
Summary: The study investigated the expression patterns of genes involved in LC-PUFA biosynthesis during early life stages of the tropical gar Atractosteus tropicus, finding that the activity of Fads and Elovl enzymes is influenced by transcriptional and dietary factors. The results suggest that the tropical gar activates its LC-PUFA biosynthetic machinery to produce ARA, EPA and DHA to meet physiological demands at crucial developmental milestones during early development.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Fisheries
Md. Akibul Hasan Bakky, Ngoc Tuan Tran, Yongsheng Zhang, Hang Hu, Haitian Lin, Ming Zhang, Huifen Liang, Yueling Zhang, Shengkang Li
Summary: The dietary supplementation of red seaweed-derived polysaccharides (GLP) has positive effects on the growth, antioxidant activity, and immunity of rabbitfish. It can be used as an immunostimulant and growth promoter in rabbitfish aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Alberto Ribes-Navarro, Juan C. Navarro, Francisco Hontoria, Naoki Kabeya, Inger B. Standal, Jan O. Evjemo, Oscar Monroig
Summary: The study revealed that gammarids have at least three distinct Elovl genes, with Elovl4 and Elovl1/7-like playing key roles in polyunsaturated fatty acid elongation, while Elovl6 does not play a major role in LC-PUFA biosynthesis in gammarids.
Article
Biochemistry & Molecular Biology
Yongnan Li, Douglas R. Tocher, Yuning Pang, Jianlong Du, Xiaojun Xiang, Kangsen Mai, Qinghui Ai
Summary: This study compared the transcriptional regulation of the critical enzyme, elovl5, in the LC-PUFA biosynthesis pathway in marine large yellow croaker and freshwater rainbow trout. The expression of elovl5 and chromatin accessibility in the regulatory region were higher in rainbow trout than in large yellow croaker. Additionally, differences in the sequence and activity of the elovl5 promoter were observed between the two species, as well as differences in the regulatory roles of transcription factors. These findings suggest that changes in the gene regulatory region driven by natural selection likely play a key role in differences in elovl5 expression and activity, influencing the LC-PUFA biosynthesis capacities of rainbow trout and large yellow croaker.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Fisheries
Camila Fernandes Correa, Renata Oselame Nobrega, Bruna Mattioni, Giovanni M. Turchini, Debora Machado Fracalossi
Summary: This study evaluated the effects of different lipid sources on the in vivo fatty acid metabolism in Nile tilapia, and found that the enzyme activities and fatty acid composition were significantly affected by the diets and environmental temperatures.
Article
Agriculture, Multidisciplinary
Jiaxiang Luo, Oscar Monroig, Kai Liao, Alberto Ribes-Navarro, Juan Carlos Navarro, Tingting Zhu, Juan Li, Liangli Xue, Qicun Zhou, Min Jin
Summary: The study demonstrates that Elovl4 enzymes in silver pomfret play crucial roles in the biosynthesis of long-chain and very-long-chain polyunsaturated fatty acids. Acute salinity changes influence the biosynthesis of LC-PUFAs in silver pomfret, with low salinity stress potentially enhancing this process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Fisheries
Marc Ramos-Llorens, Alberto Ribes-Navarro, Juan C. Navarro, Francisco Hontoria, Naoki Kabeya, Oscar Monroig
Summary: The study demonstrates that Artemia franciscana has a high elongation capacity but limited overall LC-PUFA biosynthesis due to the lack of an adequate complement of fatty acyl desaturases in its genome.
Article
Fisheries
Hanlin Xu, Shajahan Ferosekhan, Serhat Turkmen, Juan Manuel Afonso, Maria Jesus Zamorano, Marisol Izquierdo
Summary: The study found significant trans-generational effects of both the broodstock fads2 expression and the type of lipid in the broodstock diet on the metabolism and performance of the juvenile progeny challenged with a diet low in FM and FO.
Article
Agriculture, Multidisciplinary
Dizhi Xie, Junfeng Guan, Xiaoping Huang, Chao Xu, Qing Pan, Yuanyou Li
Summary: The study demonstrates that farmed tilapia can be a beneficial source of n-3 LC-PUFA due to its high biosynthetic capacity of n-3 LC-PUFA in the intestine and liver.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Food Science & Technology
Karsoon Tan, Huaiping Zheng
Summary: This paper reviews the research on LC-PUFA biosynthesis in mollusks and summarizes the relevant data to improve understanding in this field. It has significant implications for guiding future studies and improving the health and survival rate of mollusk larvae.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Yewei Dong, Zhiyong Xie, Cuihong You, Mengmeng Li, Yang Li, Jianhong Zhao, Dizhi Xie, Shuqi Wang, Yuanyou Li
Summary: This study investigated the regulatory role of the GPR120 signaling pathway in LC-PUFA biosynthesis in rabbitfish. The results showed that GPR120 agonists treatment led to lower DHA content and mRNA levels of key genes involved in LC-PUFA biosynthesis. Transcriptome analysis and signaling chemical treatments confirmed the involvement of the ERK1-Srebp1c signaling pathway. These findings reveal a novel regulatory mechanism in vertebrates for LC-PUFA biosynthesis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Richard Smith, Juliette Jouhet, Chiara Gandini, Vladimir Nekrasov, Eric Marechal, Johnathan A. Napier, Olga Sayanova
Summary: The marine diatom Phaeodactylum tricornutum is a model organism for studying long chain polyunsaturated fatty acids biosynthesis. The study identified the key enzyme plastidial acyl carrier protein Delta 9-desaturase and revealed its important role in EPA synthesis and triacylglycerol production. This research provides insight into lipid metabolism in diatoms and suggests novel approaches for manipulating oil composition in microalgae.
Article
Microbiology
Ruigang Yang, Hui Wang, Lingyun Zhu, Lvyun Zhu, Tianzhong Liu, Dongyi Zhang
Summary: This study identified a novel candidate gene encoding the chloroplast stromal acyl-acyl carrier protein Delta(9) desaturase in the oleaginous marine microalga Nannochloropsis oceanica. RT-qPCR results showed upregulated transcriptional abundance of this gene under nitrogen starvation condition. The expressed protein displayed desaturated capacity for palmitoyl-ACP (C16:0-ACP) and stearoyl-ACP (C18:0-ACP), and functional complementation assay confirmed its role in oleic acid biosynthesis.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Fisheries
Cuihong You, Baojia Chen, Mei Zhang, Yiru Shao, Shuqi Wang, Cuiying Chen, Li Lin, Yanhua Huang, Meng Zhou, Yewei Dong, Yuanyou Li
Summary: The study found that dietary n-3 LC-PUFA, especially EPA-enriched oil, can improve intestinal health and decrease oxidative stress indicators in Trachinotus ovatus. Additionally, EPA and DHA-enriched diets can modulate the expression of intestinal mucosal-related genes and regulate the proportions of probiotics and beneficial bacteria.
AQUACULTURE NUTRITION
(2021)
Article
Fisheries
Bo Shi, Fangmin Xu, Qicun Zhou, Melanie K. Regan, Monica B. Betancor, Douglas R. Tocher, Mihai Sun, Fanyi Meng, Lefei Jiao, Min Jin
Summary: The study showed that dietary organic zinc significantly improved the growth performance, mineral bioaccumulation, innate immune response, and antioxidant capacity of juvenile Pacific white shrimp. The optimal dietary zinc requirement was estimated to be 104.8 mg kg(-1).
AQUACULTURE REPORTS
(2021)
Article
Chemistry, Medicinal
Manuel Marrero, Oscar Monroig, Monica Betancor, Marcelino Herrera, Jose A. Perez, Diego Garrido, Ana Galindo, Inmaculada Giraldez, Covadonga Rodriguez
Summary: This study aims to evaluate the combined effects of dietary composition and salinity on the fatty acid composition and enzymatic activity in different body compartments of fish. The findings show that dietary composition primarily regulates the expression of fads2, while salinity plays a stronger role in regulating elovl5.
Review
Biochemistry & Molecular Biology
Dizhi Xie, Cuiying Chen, Yewei Dong, Cuihong You, Shuqi Wang, Oscar Monroig, Douglas R. Tocher, Yuanyou Li
Summary: This article reviews progress on LC-PUFA biosynthesis in teleosts, focusing on the identified Fads and Elovl, as well as the regulatory mechanisms at transcriptional and post-transcriptional levels. Insights into improving endogenous LC-PUFA biosynthesis capacity to reduce dependence on fish oil while maintaining or increasing flesh LC-PUFA content and the nutritional quality of farmed fish are provided.
PROGRESS IN LIPID RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Xiangce Li, Yewei Dong, Qiuxuan Sun, Xiaohong Tan, Cuihong You, Yanhua Huang, Meng Zhou
Summary: This study evaluated the influence of different dietary fat sources and levels on the growth and fatty acid composition of black soldier fly larvae. The results showed that sufficient supply of C16:0, C18:0, and C18:3n-3 is beneficial for larvae growth, while dietary fat sources and levels significantly affect the nutrient composition of larvae.
Article
Agriculture, Multidisciplinary
Q. X. Sun, X. C. Li, X. H. Tan, Y. W. Dong, C. H. You, H. L. Yuan, Y. Y. Lu, Y. H. Huang, M. Zhou
Summary: This study investigated the digestive adaptation of black soldier fly larvae to different organic wastes, revealing that growth performance, gut environment, digestive enzyme activity, and intestinal bacterial composition were influenced by the food sources.
JOURNAL OF INSECTS AS FOOD AND FEED
(2022)
Article
Biochemistry & Molecular Biology
Lijie Liu, Cuiying Chen, Yewei Dong, Yu Cheng, Cuihong You, Shuqi Wang, Hongyu Ma, Yuanyou Li
Summary: This study showed that insulin could activate the biosynthesis of LC-PUFA in rabbitfish hepatocytes through the PI3K/Akt/mTOR/Srebp1 pathway, indicating a potentially important regulatory mechanism for lipid metabolism in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
B. T. Wang, H. L. Wu, Y. W. Dong, B. Jiang, W. Li, Y. L. Su, Y. H. Huang, C. Liu
Summary: The substitution of fishmeal with fresh black soldier fly larvae can affect the growth, serum and liver parameters, intestinal enzyme activity, histomorphology, and disease resistance of Largemouth bass.
JOURNAL OF INSECTS AS FOOD AND FEED
(2022)
Article
Genetics & Heredity
Yewei Dong, Lijie Liu, Mengmeng Li, Dizhi Xie, Jianhong Zhao, Shuqi Wang, Cuihong You, Yuanyou Li
Summary: The study found that the rabbitfish is the first marine teleost with the ability to synthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C-18 precursors through the regulation of insulin response elements (IREs) with the involvement of transcription factors Srebp-1c and Sp1.
Article
Biochemistry & Molecular Biology
Yewei Dong, Zhiyong Xie, Cuihong You, Mengmeng Li, Yang Li, Jianhong Zhao, Dizhi Xie, Shuqi Wang, Yuanyou Li
Summary: This study investigated the regulatory role of the GPR120 signaling pathway in LC-PUFA biosynthesis in rabbitfish. The results showed that GPR120 agonists treatment led to lower DHA content and mRNA levels of key genes involved in LC-PUFA biosynthesis. Transcriptome analysis and signaling chemical treatments confirmed the involvement of the ERK1-Srebp1c signaling pathway. These findings reveal a novel regulatory mechanism in vertebrates for LC-PUFA biosynthesis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jens Boyen, Alberto Ribes-Navarro, Naoki Kabeya, Oscar Monroig, Annelien Rigaux, Patrick Fink, Pascal I. Habluetzel, Juan Carlos Navarro, Marleen De Troch
Summary: Long-chain (>= C-20) polyunsaturated fatty acids (LC-PUFAs) are physiologically important for most animals, including humans. Recent research indicates the ability of certain invertebrates, mainly marine organisms, to synthesize and convert LC-PUFAs. The genetic pathways and mechanisms of LC-PUFA biosynthesis remain unknown in many invertebrates, but existing genomic resources can contribute to our understanding. This study identified key enzymes responsible for LC-PUFA biosynthesis in the copepod Platychelipus littoralis and demonstrated their functionality using heterologous expression in yeast.
Article
Biochemistry & Molecular Biology
Pauline Wischhusen, Monica B. Betancor, Matthew Sprague, Aurelio Ortega, Fernando de la Gandara, Douglas R. Tocher, Gabriel Mourente
Summary: This study investigated the effect of dietary selenium supplementation on physiological parameters, selenoprotein and antioxidant enzyme gene expression in Atlantic bluefin tuna larvae. It was found that feeding a diet containing 4.42 μg Se/g dry mass improved the expression of selenoproteins and antioxidant genes. This suggests that non-selenium-enriched rotifers may be suboptimal for first-feeding Atlantic bluefin tuna larvae.
Article
Agriculture, Dairy & Animal Science
Jingjing Lu, Xinyue Tao, Jiaxiang Luo, Tingting Zhu, Lefei Jiao, Peng Sun, Qicun Zhou, Douglas R. Tocher, Min Jin
Summary: This study aimed to investigate the effects of dietary choline supplementation on Pacific white shrimp. The results showed that dietary choline supplementation promoted the deposition of choline and its metabolites, had negative effects on the histological structure and fatty acid profile of the hepatopancreas, and regulated lipid metabolism. Appropriate dietary choline levels promoted the development of hepatopancreas R cells and maintained normal lipid accumulation, while high dietary choline levels enhanced lipid export and fatty acid oxidation, and inhibited de novo fatty acid synthesis.
Article
Fisheries
Monica B. Betancor, Matthew Sprague, Daniel Gonzalez-Silvera, Aurelio Ortega, Fernando de la Gandara, Xu Gong, Johnathan A. Napier, Douglas R. Tocher, Gabriel Mourente
Summary: Recently, genetically modified Camelina sativa has been used to produce oils rich in omega-3 fatty acids, which can potentially replace fish oil in the feeds for juvenile Atlantic Bluefin tuna. The study found that the oils showed positive effects on the growth, survival, and feed utilization of the tuna. Furthermore, the oils upregulated the expression of genes related to lipid metabolism.
Article
Agricultural Economics & Policy
Cecile Brugere, Krishna Pillai Padmakumar, William Leschen, Douglas R. Tocher
Summary: The study reveals that fish farmers familiar with manufactured feed have a more positive attitude towards feed containing non-conventional ingredients. Peer pressure, importance and benefits of the novel aquafeed, perceived comparative advantage, and uncertainty regarding outcomes are the main determinants of intention to adopt the new feed innovation.
AQUACULTURE ECONOMICS & MANAGEMENT
(2021)