Article
Fisheries
Reoto Tani, Ryosuke Yazawa, Shigeharu Kamio, Wataru Kawamura, Tetsuro Morita, Yutaka Takeuchi, Goro Yoshizaki
Summary: In this study, the feasibility of using germ cell-less hybrids as recipients for surrogate broodstock technology in mackerels was examined. Results showed that male recipients could produce donor-derived sperm, while some females could produce donor-derived eggs. Notably, successful fertilization and hatching of normal offspring were observed in the experiment.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Roman Franek, Vojtech Kaspar, Mujahid Ali Shah, David Gela, Martin Psenicka
Summary: Recent progress in reproductive biotechnology in fishes has allowed the transfer of germ stem cells between individuals or even species, providing a valuable tool to recover cryopreserved germ stem cells.
Review
Biotechnology & Applied Microbiology
Ye Hwa Jin, Diego Robledo, John M. Hickey, Mike J. McGrew, Ross D. Houston
Summary: The use of surrogate broodstock technology provides new opportunities for improving genome editing, preserving aquatic genetic resources, and reducing generation intervals in aquaculture breeding, ultimately expediting genetic gain and dissemination of high quality production animals.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Diogenes Henrique de Siqueira-Silva, Amanda Pereira dos Santos Silva, Raphael da Silva Costa, Jose Augusto Senhorini, Alexandre Ninhaus-Silveira, Rosicleire Verissimo-Silveira
Summary: The study established a simplified protocol for the transplantation of Brycon orbignyanus spermatogonial stem cells into Astyanax altiparanae testes, leading to successful xenogenic germ cell transplantation in sexually mature Characiformes fish species.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Jun Hyung Ryu, Lan Xu, Ten-Tsao Wong
Summary: This review examines the practicality of fish germ cell transplantation and discusses its advantages, obstacles, recent advances, and future perspectives. The review also highlights the need for further technology developments to overcome the obstacles and enhance the commercial applications of this technology in aquaculture.
Article
Fisheries
Ryosuke Yazawa, Tsubasa Kubokawa, Kensuke Ichida, Wataru Kawamura, Reoto Tani, Shigeharu Kamio, Tetsuro Morita, Goro Yoshizaki
Summary: The study identified two monoclonal antibodies that can specifically recognize the germ cells of bluefin tuna. These antibodies were used to develop a method to track transplanted bluefin tuna germ cells in the gonads of xenogenic recipients. The antibodies can be a powerful tool to observe survival, proliferation, and differentiation of the transplanted germ cells in the recipient's gonads.
Article
Fisheries
Hiroyuki Yoshikawa, Yasuko Ino, Atsushi Shigematsu, Yasutoshi Yoshiura
Summary: This study demonstrates the successful enrichment of type A spermatogonia (ASG) through density gradient centrifugation, thereby enabling the production of functional gametes for surrogate broodstock technology in the tiger puffer. This method has significant implications for preserving the aquacultural genetic resources of the species.
AQUACULTURE RESEARCH
(2022)
Article
Biology
Tetsuro Morita, Misako Miwa, Naoki Kumakura, Kagayaki Morishima, Takahisa Miki, Yutaka Takeuchi, Goro Yoshizaki
Summary: The study successfully established a method for cryopreserving yellowtail spermatogonia and producing functional sperm through intraperitoneal transplantation into allogeneic recipients, demonstrating the feasibility of this technique.
Article
Fisheries
Roman Franek, Yu Cheng, Michaela Fucikova, Vojtech Kaspar, Xuan Xie, Mujahid Ali Shah, Otomar Linhart, Ivo Sauman, Martin Psenicka
Summary: Surrogate broodstock technology has potential in aquaculture and endangered species conservation. This study investigated the effects of different sterilization strategies on surrogacy using zebrafish as a model species. Germ cell-depleted recipients with normal somatic cells were identified as the most convenient sterilization method considering germline chimera rate and reproductive output.
Article
Agriculture, Dairy & Animal Science
Rosa Zupa, Edmond Hala, Gianluca Ventriglia, Chrysovalentinos Pousis, Letizia Passantino, Angelo Quaranta, Aldo Corriero, Caterina De Virgilio
Summary: The study assessed the reproductive maturation of meagre reared in sea cages under routine farming conditions. It was observed that meagre males and females, produced in hatcheries and reared in sea cages, are able to spontaneously produce and release gametes. This suggests that meagre reproduction under commercial conditions could expand meagre aquaculture production, which currently relies on limited fingerling production by a select few hatcheries.
Article
Immunology
Muhammad Kalim, Hamid Ali, Ashfaq Ur Rehman, Yong Lu, Jinbiao Zhan
Summary: This study used recombinant technology to construct recombinant molecules of PD-L1 and investigated the binding affinity of the antibodies. Protein-protein interactions were also determined using docking approach. The accuracy of 3D models was validated using SWISS and other tools.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Surintorn Boonanuntanasarn, Somkiat Sreebun, Kunlanan Booncherd, Pongsawan Khaosa-art, Treerat Sooksawat, Kensuke Ichida, Nopadon Pirarat, Ryosuke Yazawa
Summary: This study aimed to develop a cryopreservation method for spermatogonia by freezing the testicular tissue of striped catfish and testing whether cryopreserved spermatogonia exhibit transplantability. The results showed that cryoinjury of spermatogonia during cryopreservation could lead to apoptosis. Furthermore, cryopreserved spermatogonia were transplanted into allogeniec recipient larvae and compared with fresh spermatogonia, demonstrating the transplantability of cryopreserved spermatogonia.
Review
Food Science & Technology
Tingting Ma, Jiaqi Wang, Tian Lan, Shihan Bao, Qinyu Zhao, Xiangyu Sun, Xuebo Liu
Summary: This article provides an overview of the application of thermal and non-thermal techniques in the field of fruit and vegetable juice processing, discussing the impact of different technologies on the quality of the juice and the use of different combinations of hurdle technology. It is important for designing and optimizing technical parameters to produce value-added products.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biology
Therese Callet, Hongyan Li, Pascale Coste, Stephane Glise, Cecile Heraud, Patrick Maunas, Yvan Mercier, Nicolas Turonnet, Chloe Zunzunegui, Stephane Panserat, Valerie Bolliet, Lucie Marandel
Summary: The study found that parental diets could impact offspring metabolism and growth, with the composition of the diet affecting progeny's energy metabolism and epigenetic landscape. This highlights the importance of parental nutrition in shaping offspring traits.
Article
Fisheries
Kensuke Ichida, Reoto Tani, Somkiat Sreebun, Araya Jangprai, Pailin Pasomboon, Ryosuke Yazawa, Goro Yoshizaki, Surintorn Boonanuntanasarn
Summary: Selective breeding is crucial for sustainable aquaculture, and barramundi is no exception. Developing a germ cell transplantation method is essential for preserving diverse genetic characteristics. Transplanting frozen germ cells into recipients is a powerful tool for obtaining the next generation.
Article
Fisheries
Daniel Antonio Rotili, Darci Carlos Fornari, Everton Luis Zardo, Juan R. Esquivel-Muelbert, Marcelo Bernardi, Diogenes Henrique Siqueira-Silva, Danilo Pedro Streit
Summary: The levels of reproductive hormones for fish can vary with age and sex, with higher levels of E2 in immature male fish and higher levels of T in fish at 24 and 48 months. In females, E2 levels were higher across different age groups, while T and 11-KT were observed in 48 month females.
Article
Genetics & Heredity
Zuzana Blahova, Roman Franek, Marek Let, Martin Blaha, Martin Psenicka, Jan Mraz
Summary: This study used the CRISPR/Cas9 genome editing system to induce mutations in the zebrafish genome and found that fads2 gene editing may result in a decrease in egg quality. The study also revealed a new LC-PUFA biosynthesis pathway in zebrafish.
Article
Anatomy & Morphology
Jhennifer Gomes Cordeiro, Jeane Rodrigues, Raquel dos Santos, Maira da Silva Rodrigues, Rafael Henrique Nobrega, Felipe Fernando da Silva Siqueira, Caio Maximino, Diogenes Henrique de Siqueira-Silva
Summary: This study describes important components of the reproductive biology of the Amazonian amphibian fish Copella arnoldi, including sex ratio, length-weight relationship, and gametogenesis. The results show that the species is capable of spawning throughout the year, with only certain individuals capable of spawning during different seasons. Additionally, this species exhibits a rare type of semicystic spermatogenesis, which is described for the first time in the Lebiasinidae family.
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Ravindra Naraine, Ievgen Lebeda, Ales Tomcala, Mariola Dietrich, Roman Franek, Martin Psenicka, Radek Sindelka
Summary: This study analyzed the effect of DNA damage on embryo development and found significant changes in the transcriptome and proteome patterns after exposure to genotoxicants. The results suggest a correlation between phenotype formation and changes in transcriptomic and proteomic profiles. Additionally, markers of DNA damage response and adaptive stress response were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Ravindra Naraine, Viktoriia Iegorova, Pavel Abaffy, Roman Franek, Vladimir Soukup, Martin Psenicka, Radek Sindelka
Summary: The study revealed significant differences in RNA localization among different vertebrate models, indicating the highly diverse regulation of embryonic development within the animal kingdom cannot be deduced based on a single model.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Viktoriia Iegorova, Ravindra Naraine, Martin Psenicka, Monika Zelazowska, Radek Sindelka
Summary: The localization of biomolecules within the oocyte drives the spatial differentiation of daughter cells during embryogenesis. While the localization pathways of vegetal maternal transcripts have been well-described, limited information is available about the localization of animal pole transcripts. In this study, TOMO-Seq analysis on two different species was performed, revealing temporal preferences for transcript localization establishment and the presence of both conserved and species-specific transcripts. Additionally, active remodeling of the maternal RNA pool through de novo transcription and degradation was observed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Veterinary Sciences
Xuan Xie, Roman Franek, Martin Psenicka, Fan Chen, Vojtech Kaspar
Summary: This study optimized the cultivation conditions for germ cells in common carp, and the results showed that using hESC media and RTG2 cell line as feeder cells promoted germ cell proliferation and survival. Additionally, opening the carp from the dorsal side minimized the risk of contamination. These findings are important for common carp breeding, genetic resource conservation, and development of specific techniques.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Helena Robattini Carvalho, Diogenes Henrique De Siqueira-Silva, Romulo Batista Rodrigues, Louise Nex Spica, Daniel Antonio Rotili, Everton Luis Zardo, Andrea Giannotti Galuppo, Renata Villar, Juan Ramon Esquivel-Muelbert, Jayme Aparecido Povh, Leonardo Barcellos, Danilo Pedro Streit Jr
Summary: The effect of temperature on gonadal development and sex ratio in Brycon orbignyanus was assessed in this study. Fish were kept at three different temperatures (20°C, 23°C, and 26°C) from the beginning of exogenous feeding. Results showed that the sexual pattern was temperature-dependent, with undifferentiated individuals at 20°C and a higher proportion of immature males at 23°C and males in maturation process and regression phase at 26°C. Testosterone levels were quantified in all treatments, while detectable estradiol levels were found only at 23°C. Continuous exposure to 23°C and 26°C favored testicular development, with faster gonadal development observed at 26°C.
Article
Reproductive Biology
Jelena Lujic, Roman Franek, Zoran Marinovic, Vojtech Kaspar, Xuan Xie, Akos Horvath, Martin Psenicka, Bela Urbanyi
Summary: The aim of this study was to test the efficacy of needle-immersed vitrification (NIV) for preserving oogonia (OOG) in sterlet and Russian sturgeon ovarian tissue in order to enhance conservation efforts for these endangered fish species. The study found that vitrification of the ovarian tissue using specific solutions resulted in viable OOGs, which were able to successfully colonize recipient gonads after transplantation. The simplicity and practicality of the vitrification procedure make it a valuable tool for conservation work in the field.
Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Jeane Rodrigues, Maria Rosa-Silva, Hadda Tercya, Paulo Jesus, Saynara Miranda, Hingrid Oliveira, Bianca Lima, Ludmylla Santos, Caio Maximino, Diogenes Siqueira-Silva
Summary: Stress can either promote or inhibit reproduction in fish, and the release of conspecific alarm substance (CAS) after a predator attack is one stressor that affects fish. This study focused on evaluating the effects of CAS exposure on the oogenesis and reproduction of the twospot astyanax Astyanax bimaculatus. The results showed that CAS exposure did not cause visible changes in the ovaries of female fish, but it led to precocious ovulation without successful offspring, reducing breeding success compared to the control group.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Environmental Sciences
Zuzana Weiserova, Jana Blahova, Veronika Dobukova, Petr Marsalek, Nikola Hodkovicova, Jiri Lenz, Frantisek Tichy, Roman Franek, Martin Psenicka, Ales Franc, Zdenka Svobodova
Summary: This study investigated the comprehensive effects of 17 alpha-ethinylestradiol (EE2) in zebrafish, focusing on endocrine disruption, oxidative stress, and detoxification processes. Adult male triploid zebrafish were exposed to two concentrations of EE2 in their feed for six weeks. The high concentration of EE2 caused extensive mortality and significant changes in reproductive disorders, gonad histology, vitellogenin level, and selected gene expression. The environmentally relevant concentration of EE2 showed no significant differences in vitellogenin but did affect antioxidant and detoxifying enzymes. This is the first study to report the effects of subchronic oral exposure to EE2 in adult triploid zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Reproductive Biology
Thaiza Rodrigues de Freitas, Andrea Giannotti Galuppo, Lis Santos Marques, Romulo Batista Rodrigues, Maritza Perez Atehortua, Thales Souza Franca, Nathalia dos Santos Teixeira, Wanderson Valente dos Santos, Itamar Cossina Gomes, Adrieli Sachett, Hadda Tercya, Diogenes Henrique de Siqueira Silva, Douglas Gamba, Tiantian Zhang, Danilo Pedro Streit Jr
Summary: The efficiency of sodium alginate hydrogel encapsulation technique during zebrafish ovarian tissue vitrification was evaluated. The results showed that the technique maintained the membrane integrity but did not have a cryoprotective effect. Halving the cryoprotectant concentration during encapsulation did not increase lipid peroxidation, and it provided support for oocytes after in vitro maturation.
Article
Anatomy & Morphology
Maria Clara Rosa Silva, Jeane Rodrigues, Hadda Tercya Lima Silva, Paulo de Souza Jesus, Cristiane Vieira da Cunha, Keid Nolan Silva Souza, Larissa Nunes de Oliveira, Ronaldo Barros Macena, Caio Maximino, Diogenes Henrique de Siqueira-Silva
Summary: The reproductive cycle of Hemiodus unimaculatus in the upstream area of the Tucurui reservoir was studied. The results showed that the species goes through development phases in fall and winter, and spawning capable phases in spring and summer. Despite fishing restrictions, the species' population is declining in the upstream area of the reservoir, likely due to the incomplete coverage of the closed fishing season and the imbalance in the sex ratio.
Article
Multidisciplinary Sciences
Jan Stundl, Megan L. Martik, Donglei Chen, Desingu Ayyappa Raja, Roman Franek, Anna Pospisilova, Martin Psenicka, Brian D. Metscher, Ingo Braasch, Tatjana Haitina, Robert Cerny, Per E. Ahlberg, Marianne E. Bronner
Summary: Neural crest cells in ray-finned fish can differentiate into osteoblasts that form the bony part of the dermal armor, and these cells retain a neural crest gene signature. This discovery is significant for understanding the origin and evolution of skeletal structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)