Article
Microbiology
Zhicheng Huang, Jinyan Gao, Chunyan Peng, Jingjing Song, Zongsheng Xie, Jixin Jia, Haochen Li, Shumiao Zhao, Yunxiang Liang, Bin Gong
Summary: In this study, the gut microbiota of Nile tilapia was analyzed before and after feeding with different concentrations of Chlorella vulgaris. The results showed that only long-term feeding of 2% C. vulgaris significantly increased the diversity of the gut microbiota. Additionally, the composition of the gut microbiota was altered by the addition of 2% C. vulgaris. This study provides valuable insights into the impact of C. vulgaris on the gut microbiota in fish.
Article
Chemistry, Analytical
Zheng Lin Tan, Masato Yasuura, Yukichi Horiguchi, Hiroki Ashiba, Takashi Fukuda
Summary: This paper presents a new method called gel capsule-based digital PCR (gc-dPCR), which improves the stability of hydrogel capsules compared to droplets. This method enhances the sensitivity and stability of ddPCR, reduces its cost, and provides a more accurate quantification of low-concentration samples.
Article
Nutrition & Dietetics
Priyanka Velankanni, Seok-Ho Go, Jong Beom Jin, Jin-Soo Park, Sunhee Park, Su-Bin Lee, Ho-Keun Kwon, Cheol-Ho Pan, Kwang Hyun Cha, Choong-Gu Lee
Summary: Chlorella vulgaris is a unicellular green algae consumed worldwide as a functional food. This study validated its immune regulatory role through the expansion of regulatory T cell population and modulation of gut microbiota composition. The intervention of C. vulgaris alleviated the symptoms of colitis in mice and increased Treg levels, suggesting its potential in managing inflammatory bowel disease.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Virology
Imene Ben Dhifallah, Kaouther Ayouni, Haifa Jmel, Wafa Kammoun, Kamel Hamzaoui, Amel Sadraoui, Henda Triki
Summary: The study revealed that the IL-12B gene polymorphism is significantly associated with an increased risk of chronic hepatitis B (CHB) in patients, particularly in males under the age of 50.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Fisheries
Bradley M. Richardson, Matt J. Griffin, Michael E. Colvin, David J. Wise, Cynthia Ware, Charles C. Mischke, Terrence E. Greenway, Todd S. Byars, Larry A. Hanson, Mark L. Lawrence
Summary: This study examines the impact of an atypical strain of Aeromonas hydrophila in channel catfish aquaculture, providing insights into rapid field sampling methods and the importance of accurate quantitative PCR detection. Results show that the location of swab collection (gill vs. lower intestine) on pond-raised catfish is affected by the pond's condition, with implications for prevalence estimates and outbreak risk assessment. Furthermore, the study highlights the presence of a carrier state of the pathogen in apparently healthy fish, suggesting avenues for future research on disease mechanisms in catfish aquaculture.
Article
Biochemistry & Molecular Biology
Chih-Wei Lin, Chung-Hwan Chen, Meng-Hsing Wu, Fong-Ming Chang, Lin Kang
Summary: This study investigated the association between two promoter polymorphisms in the TNF genes and preeclampsia (PE). It found that the polymorphism at the -308 position in the TNF-a gene promoter region is associated with severe PE in Taiwanese Han populations.
Review
Medicine, General & Internal
Edinson Dante Meregildo-Rodriguez, Martha Genara Asmat-Rubio, Gustavo Adolfo Vasquez-Tirado
Summary: Tuberculosis is a global public health emergency. Improved diagnostic methods are urgently needed to address this epidemic. Droplet digital PCR (ddPCR) shows comparable diagnostic performance to qPCR for pulmonary tuberculosis, and better performance for extrapulmonary tuberculosis.
FRONTIERS IN MEDICINE
(2023)
Article
Immunology
Laura A. Barcan, Astrid Smud, Susana A. Besuschio, Patricia L. Giorgio, Elena Temporiti, Claudia Salgueira, Maria V. Pinoni, Claudia Nagel, Alejandro G. Schijman
Summary: This study highlights the importance of early detection of Chagas disease in solid organ transplant recipients through qPCR, with a rapid and favorable treatment outcome and no post-treatment relapse episodes observed among patients.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Jay Min Lim, Rahul Tevatia, Ravi F. Saraf
Summary: This study found that small nucleic acids have a significant impact on the limit of quantification in qPCR analysis, primarily due to the length of the PCR product. Therefore, it is crucial to consider the size of the nucleic acids and the length of the PCR product during primer design for qPCR.
Article
Hematology
Katherine M. Dominy, Simone Claudiani, Matthew O'Hare, Richard Szydlo, Gareth Gerrard, Pierre Foskett, Letizia Foroni, Dragana Milojkovic, Jane F. Apperley, Jamshid Khorashad
Summary: The introduction of tyrosine kinase inhibitor treatment has greatly improved the clinical outcome of chronic myeloid leukaemia patients. However, the different transcripts may have an impact on qPCR results, but our study showed that it does not affect patient management or outcome.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Plant Sciences
Adam C. Faller, Dhivya Shanmughanandhan, Subramanyam Ragupathy, Yanjun Zhang, Zhengfei Lu, Peter Chang, Gary Swanson, Steven G. Newmaster
Summary: Traditional botanical protein sources like peas and soybeans face new challenges in authentication as powdered supplements. The use of qPCR provides an efficient and economical method for identifying the botanical source of protein products and quantifying relative proportions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mohamad-nabi Moradi, Hamid Behrouj, Behnam Alipoor, Nejat Kheiripour, Hadi Ghasemi, Hassan Ghasemi
Summary: The study found that C. vulgaris significantly reversed the effects of NAFLD-related complications induced by a high-fat diet, especially at a 10% dose. C. vulgaris may improve NAFLD by modulating inflammatory gene expression, alleviating insulin resistance, and improving dyslipidemia. Scientists may find the results useful in developing effective supplements from C. vulgaris to address NAFLD-related complications.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jackson Alves da Silva Queiroz, Tarcio Peixoto Roca, Rutilene Barbosa Souza, Luiz Fellype Alves de Souza, Ana Maisa Passos-Silva, Andre Luiz Ferreira da Silva, Eugenia de Castro e Silva, Lourdes Maria Pinheiro Borzacov, Rita de Cassia Pontello Rampazzo, Soraya dos Santos Pereira, Thor Oliveira Dantas, Janaina Mazaro, Livia Melo Villar, Juan Miguel Villalobos Salcedo, Daniel Archimedes da Matta, Deusilene Vieira
Summary: This study developed a one-step RT-qPCR method for detecting hepatitis Delta virus, which was proven to be highly efficient and reproducible. The method can be used to monitor the disease progression and assess the effectiveness of treatment in hepatitis Delta patients.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Rasha M. Reda, Nagy M. El Gaafary, Ali A. Rashwan, Fatma Mahsoub, Nashwa El-Gazzar
Summary: The study evaluated the use of olive stone biochar (OSB) to adsorb and remove inorganic mercury from Nile tilapia aquaculture systems. Results showed that adding 2 g OSB L-1 to water every 48 hours reduced the immunosuppressive effects of mercury toxicity and lowered mercury residues in fish muscle.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Rasha M. Reda, Abdelhakeem El-Murr, Yasser Abd Elhakim, Wessam El-Shahat
Summary: The study identified Aeromonas veronii as the main pathogen in tilapia summer mortality outbreaks. Results showed that including 1% formic acid in the diet can reduce mortality and alleviate the pathogenic effects of the bacterium on the overall health status of tilapia fish.
AQUACULTURE RESEARCH
(2022)
Article
Medicine, Legal
Yasmina M. Abd-Elhakim, Bothina H. F. Omran, Shimaa A. Ezzeldein, Amany Ahmed, Nabela El-Sharkawy, Amany Abdel-Rahman Mohamed
Summary: The relative expression levels of HMGB1, TLR2, and TLR4 in rat skin wounds showed a time-dependent relationship with wound age, making them reliable indicators for wound age determination in living subjects.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Correction
Biology
Nashwa El-Gazzar, Taghreed N. Almanaa, Rasha M. Reda, M. N. El Gaafary, A. A. Rashwan, Fatma Mahsoub
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biology
Mohammad Mominur Rahman, Mohammad Rezaul Islam, Mohammad Touhidul Islam, Mohammad Harun-Or-Rashid, Mahfuzul Islam, Sabirin Abdullah, Mohammad Borhan Uddin, Sumit Das, Mohammad Saidur Rahaman, Muniruddin Ahmed, Fahad A. Alhumaydhi, Talha Bin Emran, Amany Abdel-Rahman Mohamed, Mohammad Rashed Iqbal Faruque, Mayeen Uddin Khandaker, Gomaa Mostafa-Hedeab
Summary: This review highlights the potential of stem cell-based therapies for neurological disorders and the challenges they face. Advances in laboratory cultivation technologies have successfully generated neurons and glial cells, driving the development of stem cell-based transplantation therapies for human patients.
Article
Fisheries
Rasha M. Reda, Giulia Maricchiolo, Grazia Marina Quero, Marco Basili, Frank M. Aarestrup, Lidia Pansera, Simone Mirto, Amir H. Abd El-Fattah, Mahmoud Alagawany, Afaf N. Abdel Rahman
Summary: RPC can replace up to 25% of fishmeal protein in the diet of O. niloticus, while improving the antioxidant capacity, immunocompetence, and disease resistance of the fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
Naief Dahran, Yasmina M. Abd-Elhakim, Amany Abdel-Rahman Mohamed, Marwa M. Abd-Elsalam, Enas N. Said, Mohamed M. M. Metwally, Ahmed E. Abdelhamid, Bayan A. Hassan, Mohammed Alsieni, Manal E. Alosaimi, Maram H. Abduljabbar, Eman S. El-Shetry
Summary: Green synthesized zinc oxide nanoparticles (MO-ZnONP) can effectively mitigate the neurobehavioral and neurotoxic effects induced by acrylamide (ACR).
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gomaa Mostafa-Hedeab, Amany Behairy, Yasmina M. Abd-Elhakim, Amany Abdel-Rahman Mohamed, Ahmed E. Noreldin, Naief Dahran, Rasha A. Gaber, Leena S. Alqahtani, Walaa M. Essawi, Areej A. Eskandrani, Eman S. El-Shetry
Summary: This study investigated the protective role of green synthesized zinc oxide nanoparticles (MO-ZNPs) in acrylamide (ACR)-induced reproductive dysfunctions in male rats. The findings indicated that MO-ZNPs effectively ameliorated the ACR-induced decline in reproductive parameters and hormonal imbalance, as well as sperm analysis disruption. Additionally, MO-ZNPs showed potential in reducing oxidative stress and apoptosis, while enhancing steroidogenesis and sex hormone levels, thereby protecting male rats from ACR-induced reproductive toxicity.
Article
Food Science & Technology
Hala Mahfouz, Naief Dahran, Amany Abdel-Rahman Mohamed, Yasmina M. Abd El-Hakim, Mohamed M. M. Metwally, Leena S. Alqahtani, Hassan Abdelraheem Abdelmawlla, Hazim A. Wahab, Ghalia Shamlan, Mohamed A. Nassan, Rasha A. Gaber
Summary: This study develops a more reliable method for the bio-fabrication of zinc oxide nanoparticles (ZNPs) using Moringa Oleifera extract as a chelating agent to remove acrylamide contamination. The effects of acrylamide on glutathione redox dynamics, liver function, lipid profile, and zinc residues in rats are investigated.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Medicine, Research & Experimental
Hosny A. Ibrahim, Abd El-Alim F. Abd El-Alim, Mai Abd El-Hafeez, Mohamed M. M. Metwally, Tarek Khamis, Azza A. A. Galal
Summary: This study investigated the effects of baicalein (BAI) on enhancing the anticancer potential of capecitabine (CAP) in MCF-7 cells and its protective effect against CAP-induced cardiotoxicity in female Wistar rats. In vitro experiments showed that co-treatment of BAI with CAP significantly reduced cell viability, improved cytotoxicity, altered cell cycle progression, and affected the expression of BAX and Bcl2 genes. In vivo experiments revealed that CAP administration elevated cardiac markers and inflammatory cytokines, while reducing antioxidant capacity and causing cardiac tissue alterations. Simultaneous administration of BAI and CAP improved these alterations and modulated related pathways.
Article
Biochemistry & Molecular Biology
Safaa I. Khater, Taghreed N. Almanaa, Doaa M. Abdel Fattah, Tarek Khamis, Mona M. Seif, Naief Dahran, Leena S. Alqahtani, Mohamed M. M. Metwally, Mahmoud Mostafa, Raghad A. Albedair, Azza I. Helal, Manal Alosaimi, Amany Abdel-Rahman Mohamed
Summary: In this study, Lip-BBR was found to have beneficial effects in improving hepatic damage and steatosis, insulin homeostasis, and regulating lipid metabolism in T2DM. The treatment restored liver tissue microarchitectures, reduced steatosis and liver function, and promoted autophagy through the activation of LC3-II and Bclin-1 proteins and the AMPK/mTOR pathway. Additionally, Lip-BBR activated GLP-1 expression, decreased endoplasmic reticulum stress, oxidative stress, and inflammation.
Article
Biochemistry & Molecular Biology
Ahmed Abdou Said, Rasha M. Reda, Mohamed M. M. Metwally, Heba M. Abd El-Hady
Summary: This study investigated the effects of Coriandrum sativum on the control of Aeromonas veronii infection in Oreochromis niloticus. In vitro testing showed that Coriandrum sativum extract had a zone of inhibition of 43 mm against A. veronii. In vivo experiments demonstrated that fish administered a therapeutic diet supplemented with Coriandrum sativum extract showed significant improvements in various parameters, including hematological, biochemical, and immunological parameters, as well as antioxidant capacity.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Amany Abdel-Rahman Mohamed, Amany Behairy, Yasmina M. Abd El-Hakim, Mohamed M. M. Metwally, Tarek Khamis, Samah S. Abuzahrah, Ahmed E. Abdelhamid, Leena S. Alqahtani, Walaa M. Essawi, Badriyah S. Alotaibi, Manal Alosaimi, Rehab Ahmed Ahmed El-Shaer, Marwa Mahmoud Awad, Eman S. El-Shetry
Summary: This study explores the potential toxic effects of subchronic exposure to fenpropathrin (FNP) on the reproductive system of male SD rats, and compares the effects of curcumin (CUR) and curcumin-encapsulated chitosan nanoparticles (Cs.CUR.NPs) on these toxic effects. The study involved a cohort of sixty male SD rats, and the findings showed that FNP caused oxidative stress, sperm abnormalities, reduced motility and sperm count, as well as decreased levels of sex hormones. However, treatment with CUR and Cs.CUR.NPs exhibited remarkable restorative effects on semen quality, sex hormone levels, and antioxidant capacity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Environmental Sciences
Rasha M. Reda, Rania M. A. Helmy, Ali Osman, Farag A. Gh. Ahmed, Gamila A. M. Kotb, Amir H. Abd El-Fattah
Summary: This study evaluated the protective effects of Moringa oleifera leaf ethanolic extract (MOE) on Nile tilapia exposed to a sub-lethal concentration of abamectin (ABM). MOE supplementation ameliorated hepato-renal damage, enhanced antioxidant activity and innate immune responses, and upregulated anti-inflammatory gene expression in ABM-exposed fish. Therefore, MOE could be used to counteract the oxidative stress and immune response disruption induced by ABM exposure in Oreochromis niloticus and reduce its accumulation in fish tissues.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Education, Special
Amany Abdelaleem Mohamed, Mohamed Adel Elhawary, Samah Attia El Shemy
Summary: Children with autism exhibit deficits in gross motor performance and have lower bone mineral density compared to typically developing children.
INTERNATIONAL JOURNAL OF EARLY CHILDHOOD SPECIAL EDUCATION
(2022)