期刊
FERTILITY AND STERILITY
卷 109, 期 4, 页码 675-+出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2017.11.036
关键词
Angiogenesis; endometriosis; inflammation; microRNA; peritoneal fluid
资金
- Fundacion Espanola de Trombosis y Hemostasia
- Roche Pharma Spain
- Instituto de Salud Carlos III-Fondos FEDER
- Instituto de Salud Carlos III
- Fondo Europeo de Desarrollo Regional (FEDER) Union europea, Una manera de hacer Europa [PI14/01309, PI17/01945]
- Fundacion Espanola de Trombosis y Hemostasia (FETH)
- Roche Farma Espana Stop Brain Drain-IIS La Fe [2017/0055]
Objective: To define the microRNA (miRNA) profile and its relationship with cytokines content in peritoneal fluid (PF) from endometriosis patients. Design: Case-control study. Setting: University hospital, research institute. Patient(s): One hundred twenty-six women with endometriosis (EPF) and 45 control women (CPF). Main Outcomes Measure(s): MiRNA arrays were prepared from six EPF and six CPF. Quantitative reverse transcription-polymerase chain reaction validation of nine selected miRNAs (miR-29c-3p, -106b-3p, -130a-3p, -150-5p, -185-5p, -195-5p, -451a, -486-5p, and -1343-5p) was performed. Vascular endothelial growth factor-A (VEGF-A), thrombospondin-1 (TSP-1), urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), matrix metalloproteinase-3 (MMP3), tissue inhibitor of metalloproteinases type 1 (TIMP-1), interleukin (IL)-6, IL-8, IL-17A, macrophage inflammatory protein 1 beta (MIP1beta), platelet-derived growth factor a-polypeptide A, and regulated on activation, normal T cell expressed and secreted (RANTES) were quantified by ELISA and MILLIPLEX. Result(s): MiRNA arrays showed 126 miRNAs differentially expressed (fold change +/- 1.2) (78 down-regulated, 48 up-regulated) in EPF. Validation showed higher levels of miR-106b-3p, -451a, -486-5p, IL-6, IL-8, uPA, and TIMP-1 in EPF. In menstrual phase, EPF presented up-regulation of miR-106b-3p, -130a-3p, -150-5p, -185-5p, -451a, -486-5p, VEGF-A, IL-8, MIF 1 beta, uPA, and PAI-1 compared with other phases; however, CPF did not. MiRNA-486-5p was up-regulated in sterile EPF compared with sterile controls, and VEGF-A, IL-8, and TIMP-1 were increased in sterile and fertile EPF compared with fertile CPF. Conclusion(s): MiRNAs seem to be involved in the peritoneal alterations in endometriosis, suggesting new mechanisms by which ectopic lesions could implant in endometriosis patients; and to serve as biomarkers for fertility outcome prediction. (C) 2017 by American Society for Reproductive Medicine.
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