Review
Chemistry, Inorganic & Nuclear
Xiaorui Shi, Miae Won, Chu Tang, Qihang Ding, Amit Sharma, Fu Wang, Jong Seung Kim
Summary: The RNA splicing process is essential for gene expression, and monitoring and controlling it can help repair mutant genes and treat diseases. However, the lack of appropriate tools hampers the understanding of splicing regulation.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Alessandro R. Howells, Phoebe J. Welch, John Kim, Craig R. Forest, Chengzhi Shi, Xiaojun Lance Lian
Summary: A new field of genetically encoded ultrasound contrast agents in the form of gas vesicles has emerged, which could improve the specificity of medical ultrasound imaging. However, current methods of producing gas vesicle-expressing mammalian cell lines require significant cell processing time. In this study, an inducible and drug-selectable acoustic reporter gene system was used to enable gas vesicle expression in mammalian cell lines, reducing the amount of cell processing time required.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Analytical
Nicholas J. Ginga, Raleigh Slyman, Ge-Ah Kim, Eric Parigoris, Sha Huang, Veda K. Yadagiri, Vincent B. Young, Jason R. Spence, Shuichi Takayama
Summary: This study developed a perfusion system using a double-barrel glass capillary and a pressure-based pump to manipulate the luminal contents of human intestinal organoids. Real-time measurement of fluorescence intensity showed discrete changes correlating with perfusion cycles. A novel imaging analysis method was also developed to monitor GFP-expressing E. coli within the organoids.
Article
Multidisciplinary Sciences
Miao-Ping Chien, Daan Brinks, Guilherme Testa-Silva, He Tian, F. Phil Brooks, Yoav Adam, William Bloxham, Benjamin Gmeiner, Simon Kheifets, Adam E. Cohen
Summary: Photoactivated genetically encoded voltage indicators, such as NovArch, allow for high fluorescence signals, optical sectioning, and high time resolution simultaneously. These tools have been successfully applied to optical mapping of membrane voltage in acute mouse brain slices and in spontaneously active neurons in vivo.
Article
Chemistry, Multidisciplinary
Minghai Chen, Chuang Yan, Luping Zheng, Xian-En Zhang
Summary: The study developed near-infrared BiFC and TriFC systems using miRFP670nano, enabling efficient visualization of protein interactions. The sensor system showed faster maturation rate, higher photostability, and cellular stability compared to existing systems, demonstrating its potential for various applications in cell imaging studies.
Article
Biochemistry & Molecular Biology
Masafumi Shimojo, Maiko Ono, Hiroyuki Takuwa, Koki Mimura, Yuji Nagai, Masayuki Fujinaga, Tatsuya Kikuchi, Maki Okada, Chie Seki, Masaki Tokunaga, Jun Maeda, Yuhei Takado, Manami Takahashi, Takeharu Minamihisamatsu, Ming-Rong Zhang, Yutaka Tomita, Norihiro Suzuki, Anton Maximov, Tetsuya Suhara, Takafumi Minamimoto, Naruhiko Sahara, Makoto Higuchi
Summary: This study utilized bacterial dihydrofolate reductase ecDHFR and its unique antagonist TMP to facilitate imaging in the brain, enabling PET and intravital microscopy of neuronal ecDHFR expression in mice. It showed the potential for PET studies of turnover and self-assembly of proteins tagged with ecDHFR mutants. The results establish new opportunities for previously unattainable PET analyses of mammalian brain circuits at the molecular level.
Article
Neurosciences
Molly J. Kirk, Brittany R. Benlian, Yifu Han, Arya Gold, Ashvin Ravi, Parker E. Deal, Rosana S. Molina, Mikhail Drobizhev, Dion Dickman, Kristin Scott, Evan W. Miller
Summary: This study successfully combines a chemically-synthesized, voltage-sensitive fluorophore with a genetically encoded, self-labeling enzyme to enable voltage imaging in Drosophila melanogaster. Through in vivo experiments in intact fly brains, selective neuron labeling was achieved, validating the voltage sensitivity of RhoVR-Halo in fly tissue.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Chemistry, Analytical
Wenxi Xia, Shuangzhe Zhang, Jiangli Fan, Yueqing Li, Xiaojun Peng
Summary: A novel highly selective and sensitive green-emitting probe (APLN) containing a fluorophore, a linker, and a COX-2 kinase inhibitor (aspirin) has been designed and evaluated. This probe successfully labels the COX-2 enzyme to identify tumors and significantly inhibits tumor growth in a xenotransplantation model of nude mice, showing potential for in situ imaging and fluorescent-visible cancer therapy.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Jia Miao, Minqian Miao, Yue Jiang, Min Zhao, Qing Li, Yuan Zhang, Yi An, Kanyi Pu, Qingqing Miao
Summary: A NIR-II fluorescent reporter with a donor-pi-acceptor architecture was developed for specific detection of A beta plaques in AD-model mice, enabling non-invasive imaging and deep deciphering of AD progression.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Giorgia Zambito, Mary P. Hall, Monika G. Wood, Natasa Gaspar, Yanto Ridwan, Fabio F. Stellari, Ce Shi, Thomas A. Kirkland, Lance P. Encell, Clemens Lowik, Laura Mezzanotte
Summary: In this study, a new click beetle luciferase mutant, CBG2, with red-shifted color emission is developed for in vivo multicolor bioluminescence applications. Paired with NH2-NpLH2 luciferin, CBG2 (λ=660nm) and CBR2 (λ=730nm) luciferases can be used for simultaneous dual-color bioluminescence imaging in deep tissue. A spectral unmixing algorithm tool can distinguish each spectral contribution, expanding the near-infrared bioluminescent toolbox for visualization of multiple biological processes in deep tissue.
Article
Virology
Xiaozhen Li, E. Yang, Xinyu Li, Tingting Fan, Shangrui Guo, Hang Yang, Bo Wu, Hongliang Wang
Summary: This study developed fluorescent cell-based reporter systems to distinguish individual cells infected with EV71 and demonstrated their potential for studying other enterovirus-mediated cleavage and testing antiviral activity.
Review
Food Science & Technology
Siyue Wen, Yu Zhao, Mengqi Wang, Hongbin Yuan, Hengyi Xu
Summary: This study reviews the environmental properties and pollution of micro(nano)plastics (MNPs) in the human food system, as well as their potential risks to intestinal health. It discusses the sources of MNPs ingestion, the adverse effects on the intestine, and remediation strategies for MNPs pollution. The study aims to provide insights for further research on foodborne MNPs and raise public awareness of the potential risks associated with MNPs pollution in food.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jaehoon Shin, Matthew F. L. Parker, Iowis Zhu, Aryn Alanizi, Carlos I. Rodriguez, Raymond Liu, Payal B. Watchmaker, Mausam Kalita, Joseph Blecha, Justin Luu, Brian Wright, Suzanne E. Lapi, Robert R. Flavell, Hideho Okada, Thea D. Tlsty, Kole T. Roybal, David M. Wilson
Summary: This study engineered T cells with a SNIPR-based PET reporter system to track T cell trafficking. The system induced T cells to express a reporter gene cassette upon recognition of specific tumor markers. Using this system, T cells associated with target antigens were successfully imaged in vitro and in animal models.
JOURNAL OF NUCLEAR MEDICINE
(2023)
Review
Chemistry, Multidisciplinary
Qing Dan, Xinpeng Jiang, Run Wang, Zhifei Dai, Desheng Sun
Summary: Biogenic imaging contrast agents (BICAs) have unique properties that make them valuable in various in vitro and in vivo studies, such as quantification of gene expression, observation of protein interactions, visualization of cellular proliferation, monitoring of metabolism, and detection of dysfunctions. BICAs are matched with different imaging techniques, including fluorescent proteins for fluorescence imaging, gas vesicles for ultrasound imaging, and ferritin for magnetic resonance imaging. Bimodal and multimodal imaging can be achieved by combining the functions of different BICAs, overcoming the limitations of monomodal imaging.
Article
Chemistry, Analytical
Ui Jin Lee, Yunkwang Oh, Oh Seok Kwon, Yong-Beom Shin, Moonil Kim
Summary: We developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system. The system utilizes a galactose/glucose-binding protein (GGBP) with a N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The system demonstrated ultra-high sensitivity and selective responses for IAV detection, making it a simple and effective platform for IAV detection without the need for virus culture or RNA extraction processes.
Article
Ecology
Tae Young Ryu, Kwangho Kim, Tae-Su Han, Mi-Ok Lee, Jinkwon Lee, Jinhyeon Choi, Kwang Bo Jung, Eun-Jeong Jeong, Da Mi An, Cho-Rok Jung, Jung Hwa Lim, Jaeeun Jung, Kunhyang Park, Moo-Seung Lee, Mi-Young Kim, Soo Jin Oh, Keun Hur, Ryuji Hamamoto, Doo-Sang Park, Dae-Soo Kim, Mi-Young Son, Hyun-Soo Cho
Summary: The human microbiome has been found to play a crucial role in regulating colorectal cancer growth through the modulation of EHMT2 protein levels and TNFAIP1 gene expression. This study suggests that propionate and EHMT2 inhibitors may serve as therapeutic targets for colon cancer treatment, and there may be synergistic effects between EHMT2 inhibitors and the microbiome in CRC treatment.
Article
Oncology
Kunhyang Park, Kwangho Kim, Tae Young Ryu, Jinkwon Lee, Moo-Seung Lee, Mi-Young Son, Seon-Jin Lee, Young-Jun Park, Hyun-Soo Cho, Dae-Soo Kim
Summary: The present study used Gene Ontology analysis to investigate the biological responses of lung epithelial cells and fibroblasts following exposure to PM. The results suggested that PM may affect cell condition and may be associated with several human diseases.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Cell Biology
Jung Hwa Lim, Dae Hun Kim, Kyung Hee Noh, Cho-Rok Jung, Hyun Mi Kang
Summary: This study identified epidermal progenitor cells that possess unlimited proliferation and multipotent differentiation capacity. These cells can form organotypic skin models and mimic the morphologic and biochemical processes of human epidermis. The proliferation and differentiation of these cells rely on the expression of Integrin beta 1 (ITGB1). Furthermore, the in vitro and in vivo wound healing capacity of these cells was examined.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Hyun Mi Kang, Dae Hun Kim, Mijin Kim, Yoohong Min, Bohyeon Jeong, Kyung Hee Noh, Da Yong Lee, Hyun-Soo Cho, Nam-Soon Kim, Cho-Rok Jung, Jung Hwa Lim
Summary: This study discovered that the FBXL17-SPAST pathway is involved in the pathogenicity of hereditary spastic paraplegia (HSP) through loss of function and quantitative regulation. Targeting FBXL17 could provide new insights into HSP therapeutics.
CELL AND BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Seul-Gi Lee, Ye-Ji Kim, Mi-Young Son, Min-Seok Oh, Jin Kim, Bokyeong Ryu, Kyu-Ree Kang, Jieun Baek, Gujin Chung, Dong Ho Woo, C-Yoon Kim, Hyung Min Chung
Summary: In response to the growing demand for 3D culture, we studied heart organoids. By creating self-organized HOs using human iPSCs, we demonstrated their ability to mimic cardiac structures and verified their mechanical/electrophysiological features through multiple analyses. The cardiovascular characteristics of HOs were maintained after in vivo transplantation.
Article
Multidisciplinary Sciences
Jeongmin Ha, Bum Suk Kim, Byungkuk Min, Juhyeon Nam, Jae-Geun Lee, Minhyung Lee, Byoung-Ha Yoon, Yoon Ha Choi, Ilkyun Im, Jung Sun Park, Hyosun Choi, Areum Baek, Sang Mi Cho, Mi-Ok Lee, Ki-Hoan Nam, Ji Young Mun, Mirang Kim, Seon-Young Kim, Mi Young Son, Yong-Kook Kang, Jeong-Soo Lee, Jong Kyoung Kim, Janghwan Kim
Summary: The cytoskeletal linker protein desmoplakin (Dsp) plays a crucial role in both in vitro cellular reprogramming and tissue regeneration. Knockdown of Dsp impairs the formation of intermediate cells during cellular reprogramming and tissue regeneration. This study suggests a potential evolutionary link mediated by Dsp between cellular reprogramming in mammals and tissue regeneration in lower vertebrates, and the intermediate cells may provide alternative approaches for mammalian regenerative therapy.
Article
Gastroenterology & Hepatology
Hana Lee, Kwang Bo Jung, Ohman Kwon, Ye Seul Son, Eunho Choi, Won Dong Yu, Naeun Son, Jun Hyoung Jeon, Hana Jo, Haneol Yang, Yeong Rak Son, Chan-Seok Yun, Hyun-Soo Cho, Sang Kyu Kim, Dae-Soo Kim, Doo-Sang Park, Mi-Young Son
Summary: This study investigates the modulatory capacity of a newly isolated probiotic strain (DS0384) on early intestinal development. They found that DS0384 accelerated the maturation of fetal intestines and promoted intestinal stem cell proliferation by secreting a specific metabolite (NCG). The results suggest that DS0384 has potential probiotic properties and therapeutic applications for early gut development disorders.
Article
Biochemistry & Molecular Biology
Hae-Ri Lee, Soo Kim, Sungho Shin, Seon-Yeong Jeong, Dae-Won Lee, Sun-Ung Lim, Ji Yeon Kang, Mi-Young Son, Cheolju Lee, Kyung-Rok Yu, Myungshin Kim, Il-Hoan Oh
Summary: In this study, the transition from primary MSCs to iMSCs was characterized by increased proliferative activity and variations in differentiation potential. iMSCs exhibited unique gene and protein expression patterns, resembling pericyte-like cells, and showed enhanced self-renewal and immune suppression capabilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ilkyu Park, Nakyoung Kim, Sugi Lee, Kunhyang Park, Mi-Young Son, Hyun-Soo Cho, Dae-Soo Kim
Summary: The timely diagnosis of different stages in NAFLD is crucial for disease treatment and reversal. In this study, we identified differentially expressed genes in NAFLD patients compared to healthy controls and determined their predictive power. We also found significant differential expression of candidate genes in liver cancer that may influence patient survival.
Article
Engineering, Biomedical
Hyunah Ahn, Younghak Cho, Geun-Tae Yun, Kwang Bo Jung, Wonji Jeong, Yesol Kim, Mi-Young Son, Eunjung Lee, Sung Gap Im, Hee-Tae Jung
Summary: This study presents a hierarchical structure composed of nano-pillars on micro-wrinkles for enhancing cardiomyocyte maturation. The responses of cardiomyocytes on hierarchical topography are systematically investigated. The combination of nano-pillars and micro-wrinkles significantly enhances the maturation of cardiomyocytes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Kwangho Kim, Tae Young Ryu, Jinkwon Lee, Mi-Young Son, Dae-Soo Kim, Sang Kyum Kim, Hyun-Soo Cho
Summary: This study identified EHMT1 as an overexpressed gene in colorectal cancer (CRC) and demonstrated that knocking down EHMT1 expression suppressed cell growth and induced apoptosis in CRC cell lines. Further analysis revealed that EHMT1 regulates cell apoptosis through the CHOP gene. These findings suggest EHMT1 as a potential therapeutic target for CRC treatment.
MOLECULES AND CELLS
(2022)
Article
Biochemistry & Molecular Biology
Seung-Ho Park, Sung-Jin Yoon, Song Choi, Jaeeun Jung, Jun-Young Park, Young-Ho Park, Jinho Seo, Jungwoon Lee, Moo-Seung Lee, Seon-Jin Lee, Mi-Young Son, Young-Lai Cho, Jang-Seong Kim, Hyo Jin Lee, Jinyoung Jeong, Dae-Soo Kim, Young-Jun Park
Summary: The study reveals that exposure to particulate matter pollutants can induce immune cells to secrete HBEGF, leading to a more invasive state in cancer cells. This finding helps uncover the mechanism by which PM promotes cancer metastasis.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jongjin Park, Dong Gwang Lee, Na Geum Lee, Min-Gi Kwon, Yeon Sung Son, Mi-Young Son, Kwang-Hee Bae, Jangwook Lee, Jong-Gil Park, Nam-Kyung Lee, Jeong-Ki Min
Summary: In this study, researchers developed a monoclonal antibody K312 that can distinguish undifferentiated pluripotent stem cells (PSCs) from differentiated PSC progeny. By targeting with K312, pluripotent cells can be depleted from differentiated PSC progeny, effectively preventing teratoma formation.
Article
Cell & Tissue Engineering
Ohman Kwon, Won Dong Yu, Ye Seul Son, Kwang Bo Jung, Hana Lee, Mi-Young Son
Summary: This study develops a reliable culture method for 3D intestinal spheroids (InSexp) that can expand and maintain human intestinal stem cells (ISCs). By activating the WNT pathway, the InSexp can rapidly expand and be passaged. These findings contribute to the development of scalable culture systems for studying ISC biology.
INTERNATIONAL JOURNAL OF STEM CELLS
(2022)