Article
Food Science & Technology
Ahmed Y. Nassar, Fatma Y. Meligy, Gamil M. Abd-Allah, Weam A. M. Khallil, Ghadir A. Sayed, Randa T. Hanna, Gamal A. Y. Nassar, Sally M. Bakkar
Summary: Acetylated whey peptides (AWP) derived from bovine milk have been found to be a powerful iron chelator and immune modulator, making them an effective alternative to synthetic drugs for treating iron overload. The study showed that AWP significantly reduced serum iron levels, oxidative stress markers, and inflammatory markers in iron-overloaded rats, and improved histopathological changes. Moreover, it had similar efficacy to N-Acetyl-L-cysteine (NAC) in reducing iron deposition and macrophage markers expression.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Physiology
Yi-Shiou Tseng, Chun-Hou Liao, Wen-Bin Wu, Ming-Chieh Ma
Summary: The study demonstrates that NMDAR blockade alleviates D-serine-induced renal injury, possibly through the involvement of NOX4-mediated oxidative stress. These findings have important implications for preventing acute renal injury caused by D-serine.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Cell Biology
Brandon D. Anderson, Timothy Lee, Brent Bell, Ying Song, Joshua L. Dunaief
Summary: In this report, a 39-year-old patient with hereditary hemochromatosis (HH) and high serum iron levels was diagnosed with bull's eye maculopathy, a form of retinal degeneration. The low levels of ferroxidase ceruloplasmin (CP) in the patient and the high iron levels were hypothesized to be the cause of the retinal degeneration. A mouse model combining hepcidin knockout (KO) and Cp KO was used to study the role of CP in retinal degeneration, and it was found that Cp KO increased the severity of retinal degeneration. These findings suggest that CP levels may influence the severity of retinal degeneration, particularly in individuals with high serum iron levels.
DISEASE MODELS & MECHANISMS
(2023)
Article
Engineering, Chemical
Fatma Sen, Esra Altiok, Piotr Cyganowski, Joanna Wolska, Marek Bryjak, Nalan Kabay, Muserref Arda, Mithat Yuksel
Summary: The study demonstrated that the new synthesized chelating resins effectively reduced boron and arsenic concentrations in geothermal water to meet the drinking and irrigation water standards. The synthesized resins showed excellent performance in removing both species, competing with and outperforming commercial boron selective resins, making them a promising alternative for geothermal water reclamation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Richard D. Horniblow, Prachi Pathak, Dario L. Balacco, Animesh Acharjee, Eva Lles, Georgios Gkoutos, Andrew D. Beggs, Chris Tselepis
Summary: This study investigates the effect of excess dietary iron on the colonic epigenome. The findings indicate that chronic iron exposure leads to significant hypomethylation of the epigenome, with a specific effect on NRF2 pathway targets. These epigenetic modifications can be prevented by removing iron and reducing cellular lipid peroxidation. The study also provides evidence for dietary-iron mediated epigenetic alterations in vivo and suggests a link between iron status and epigenetic changes in human intestinal tissue.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Athina G. Mantelou, Alexandra Barbouti, Anna Goussia, Argyro Zacharioudaki, Alexandra Papoudou-Bai, Chara Vlachou, Stelios Kokkoris, Apostolos Papalois, Dimitrios Galaris, Georgios K. Glantzounis
Summary: The study showed that a combination of iron chelators with varying lipophilicity and iron-binding properties can enhance protection against hepatic I/R injury. Through investigations in cell lines and a rabbit model, the protective effects of these iron chelator combinations were observed.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Alexandra Barbouti, Nefeli Lagopati, Dimitris Veroutis, Vlasios Goulas, Konstantinos Evangelou, Panagiotis Kanavaros, Vassilis G. Gorgoulis, Dimitrios Galaris
Summary: The gradual accumulation of oxidatively damaged macromolecules in cells and organisms leads to cell and organ dysfunction, known as the free radical theory of aging. Lipofuscin, ceroid, or age pigment is formed through unregulated oxidative modifications induced by reactive free radicals. Modulating the distribution of labile iron pool could potentially hinder lipofuscin accumulation and cellular aging.
Article
Biochemical Research Methods
Lingge Suo, Wanwei Dai, Xuhao Chen, Xuejiao Qin, Guanlin Li, Sijia Song, Di Zhang, Chun Zhang
Summary: This study comprehensively analyzed the proteomic characteristics and modulation of the neural microenvironment in the retina and optic nerve after N-methyl-D-aspartate (NMDA)-induced neuronal degeneration, identifying proteins related to ferroptosis and autophagy. Regulating these death mechanisms simultaneously may provide maximum benefit for retinal disease therapy.
JOURNAL OF PROTEOMICS
(2022)
Article
Nutrition & Dietetics
Maria Hernandez, Sergio Recalde, Jorge Gonzalez-Zamora, Valentina Bilbao-Malave, Manuel Saenz de Viteri, Jaione Bezunartea, Maite Moreno-Orduna, Idoia Belza, Jesus Barrio-Barrio, Patricia Fernandez-Robredo, Alfredo Garcia-Layana
Summary: Combining vitamin D with Nutrof Total(R) provides synergistic protection against oxidative and inflammatory stress-induced conditions in retinal epithelial and endothelial cells, reducing cell damage and inflammation.
Article
Biochemistry & Molecular Biology
Xin Shi, Panpan Li, Hanhan Liu, Verena Prokosch
Summary: This article reviews the impact of oxidative stress (OS) on endothelial dysfunction (ED) in the retina and explores the mechanistic links between the two and their role in different retinal neurodegenerative diseases. Understanding the role of endothelial function may lead to new therapeutic approaches.
Article
Psychiatry
Kai-Chun Yang, Yen-Yu Chen, Mu-N Liu, Bang-Hung Yang, Yuan-Hwa Chou
Summary: This study found that patients with schizophrenia exhibited poorer cognitive performance and elevated concentrations of certain amino acids in plasma. The interactions between striatal dopamine transporter and NMDAR-related amino acids play a role in cognitive impairments in schizophrenia.
SCHIZOPHRENIA RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Fengping Mao, Fang Huang, Weidong Nong, Dayuan Lao, Zhuowei Gong, Wen Huang
Summary: This study aimed to evaluate the role of Cav-1 in NMDA-induced dysfunction of HBEC-5i cells and verify that endothelial NMDAR activation mediates the disruption of tight junction barrier integrity via the ERK1/2 pathway.
Article
Biochemistry & Molecular Biology
Boyoung Lee, Santosh Pothula, Min Wu, Hyeyeon Kang, Matthew J. Girgenti, Marina R. Picciotto, Ralph J. DiLeone, Jane R. Taylor, Ronald S. Duman
Summary: Researchers tested a novel NMDAR-positive modulator, NYX-783, in rodent models of PTSD and found that it significantly reduced spontaneous recovery and enhanced fear extinction. The study elucidated the cellular targets and molecular mechanisms underlying NYX-783's effects on PTSD symptoms.
MOLECULAR PSYCHIATRY
(2022)
Article
Cardiac & Cardiovascular Systems
Yang Liu, Ziqiang Luo, Zhengchang Liao, Mingjie Wang, Yan Zhou, Siwei Luo, Ying Ding, Teng Liu, Chuangding Cao, Shaojie Yue
Summary: Intrauterine hypoxia during pregnancy can increase the risk of cardiovascular disease in offspring, but the underlying mechanism is controversial. This study found that antenatal memantine treatment can protect the heart from injury in neonatal offspring exposed to intrauterine hypoxia by improving mitochondrial function and structure.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Khaled A. Osman, Eslam M. Ezz El-Din, Nabila S. Ahmed, Ayman S. El-Seedy
Summary: The study investigated the attenuating effect of N-acetylcysteine (NAC) on the toxic effects of chlropyrifos-ethyl (CPE-E) and chlropyrifos-methyl (CPF-M) in male rats, showing that NAC can ameliorate the biochemical and genotoxic markers induced by these organophosphorus compounds.
Article
Pharmacology & Pharmacy
Asami Mori, Kazuki Takeda, Kenji Sakamoto, Tsutomu Nakahara
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2020)
Article
Cell Biology
Ayuki Nakano, Ryo Kondo, Yuki Kaneko, Shiho Arima, Daiki Asano, Akane Morita, Kenji Sakamoto, Tohru Nagamitsu, Tsutomu Nakahara
CELL AND TISSUE RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Ryo Kondo, Ayuki Nakano, Daiki Asano, Akane Morita, Shiho Arima, Asami Mori, Kenji Sakamoto, Tohru Nagamitsu, Tsutomu Nakahara
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Chemistry, Medicinal
Kota Koike, Masanobu Nagano, Masahiro Ebihara, Tasuku Hirayama, Mieko Tsuji, Hiroaki Suga, Hideko Nagasawa
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Pharmacology & Pharmacy
Asami Mori, Ayumi Taniai, Mami Hasegawa, Kenji Sakamoto, Tsutomu Nakahara
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2020)
Article
Anatomy & Morphology
Akane Morita, Mika Yoshizumi, Shiho Arima, Asami Mori, Kenji Sakamoto, Tohru Nagamitsu, Tsutomu Nakahara
Summary: In a mouse model, pharmacological depletion of retinal neurons was found to delay the development of deep vascular plexus while not affecting the superficial layer. Loss of neuronal cells reduced the number of vertical sprouts from the superficial plexus without impacting the angiogenic sprouts. Neuronal cell loss did not impair fibronectin and astrocyte networks in the superficial layer.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Chemistry, Multidisciplinary
Tasuku Hirayama, Masato Niwa, Shusaku Hirosawa, Hideko Nagasawa
Article
Pharmacology & Pharmacy
Asami Mori, Erika Yano, Kenji Sakamoto, Kunio Ishii, Tsutomu Nakahara
Summary: This study demonstrated that ACh stimulates the production of EETs, which dilate retinal arterioles by activating BKCu channels. EETs may play a role in the EDH-related component of ACh-induced dilation of retinal arterioles.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Chemistry, Multidisciplinary
Kanta Kawai, Tasuku Hirayama, Haruka Imai, Takanori Murakami, Masatoshi Inden, Isao Hozumi, Hideko Nagasawa
Summary: In this study, we developed an activity-based fluorescence probe H-FluNox for monitoring the fluctuations of labile heme (LH) in cells. The probe exhibited high selectivity towards LH, enabling the discrimination of LH from labile Fe(II). By using this probe, we observed the acute release of LH upon NO stimulation and the accumulation of LH by inhibiting the heme exporter. Furthermore, the imaging studies using the probe provided insights into the heme-export activity of ATP-binding cassette subfamily G member 2 (ABCG2), the LH pooling ability of G-quadruplex, and the involvement of LH in ferroptosis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Ophthalmology
Koki Nagaoka, Yuki Kurauchi, Daiki Asano, Akane Morita, Kenji Sakamoto, Tsutomu Nakahara
Summary: Na+/K+-ATPase (NKA) plays a crucial role in maintaining the neuro-glio-vascular unit in the retina. Inhibition of NKA leads to degeneration of neuronal and vascular cells, alteration of glial cells, and disturbance of retinal glutamate metabolism and decreased VEGF expression, which may contribute to neurovascular degeneration.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yoko Hirata, Kohei Oka, Shotaro Yamamoto, Hiroki Watanabe, Kentaro Oh-hashi, Tasuku Hirayama, Hideko Nagasawa, Hiroshi Takemori, Kyoji Furuta
Summary: Haloperidol has been found to not only act as an antipsychotic agent, but also as a neuroprotective agent against endogenous oxidative stress. In addition, lysosome-targeting ferroptosis inhibitors could be useful for the treatment of various diseases.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Mieko Tsuji, Haruno Taira, Taro Udagawa, Tatsuya Aoki, Tasuku Hirayama, Hideko Nagasawa
Summary: We developed a caged hydroperoxide, BhcTBHP, which releases prooxidant TBHP upon blue light irradiation. When photoirradiated, MitoTBHP with triphenylphosphonium at position 7 selectively induces oxidative stress and membrane depolarization in mitochondria. This study presents a powerful tool for investigating redox signaling and oxidative stress in living cells.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Takumi Kariya, Hayato Hasegawa, Taro Udagawa, Yusaku Inada, Kyoko Nishiyama, Mieko Tsuji, Tasuku Hirayama, Tatsuo Suzutani, Naoki Kato, Shingo Nagano, Hideko Nagasawa
Summary: This study investigates the role of the absolute configuration of the C-6 carbon of the substrate polyene in the stereocontrol of the intramolecular Diels-Alder reaction (IMDA). The results show that Fsa2 exhibits consistent stereoselectivity, while Phm7 shows different stereoselectivity depending on the configuration of the 6-methyl group. The study also discovers a unique relationship between the stereochemistry of decalins and their antibacterial activity.
Article
Chemistry, Organic
Akari Mukaimine, Tasuku Hirayama, Hideko Nagasawa
Summary: This study presents the synthesis, photophysical properties, and photosensitizing abilities of asymmetric bismuth-incorporated rhodamines, highlighting their potential for activatable fluorogenic photosensitizers. By introducing diverse functional groups into the rhodamine structure, including a gamma-glutamyl group, a versatile scaffold for responsive photoactive agents was established with selective activation in cells with specific enzyme activity.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Organic
Ryo Kakiuchi, Tasuku Hirayama, Daijiro Yanagisawa, Ikuo Tooyama, Hideko Nagasawa
ORGANIC & BIOMOLECULAR CHEMISTRY
(2020)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)