Article
Endocrinology & Metabolism
Victor R. R. Adorno Febles, Yuan Hao, Aarif Ahsan, Jiansheng Wu, Yingzhi Qian, Hua Zhong, Stacy Loeb, Danil V. V. Makarov, Herbert Lepor, James Wysock, Samir S. S. Taneja, William C. C. Huang, Daniel J. J. Becker, Arjun V. V. Balar, Jonathan Melamed, Fang-Ming Deng, Qinghu Ren, Donald Kufe, Kwok-Kin Wong, Dennis O. O. Adeegbe, Jiehui Deng, David R. R. Wise
Summary: This study characterized the tumor immune microenvironment of localized prostate cancer, showing that high-grade prostate cancer (HGPCa) is highly infiltrated by exhausted CD8(+) T cells, myeloid cells, and regulatory T cells. In contrast, low-grade prostate cancer (LGPCa) has a high ratio of activated CD8(+) effector T cells. These findings provide important insights into the immunologic mechanisms of prostate cancer pathogenesis and the potential for immunotherapy.
Article
Environmental Sciences
Hua Chen, Anqi Song, Faisal UL Rehman, Dan Han
Summary: Despite significant advancements in cancer research, the outcomes for lung cancer patients are still unsatisfactory. In this study, we utilized single-cell RNA sequencing to identify and characterize the cellular heterogeneity within the lung cancer microenvironment. Our findings revealed distinct cell types and their interplay, identified potential predictive biomarkers, and shed light on the immune response within the tumor microenvironment. These findings contribute to our understanding of cancer heterogeneity and have implications for the development of targeted therapies.
ENVIRONMENTAL TOXICOLOGY
(2023)
Review
Oncology
Yalan Lei, Rong Tang, Jin Xu, Wei Wang, Bo Zhang, Jiang Liu, Xianjun Yu, Si Shi
Summary: Single-cell sequencing is a powerful tool that has revolutionized our understanding of the biological characteristics and dynamics within cancer lesions. Its use in cancer research provides valuable information related to malignant cell landscapes, immune cell interactions, tumor heterogeneity, circulating tumor cells, and underlying mechanisms of tumor behaviors, with bright prospects for diagnosis, targeted therapy, and prognostic prediction among various tumors. Advances in single-cell sequencing will undoubtedly enhance our understanding of tumor biological characteristics and uncover potential precise therapeutic targets for patients in the near future.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Review
Oncology
Yijie Zhang, Dan Wang, Miao Peng, Le Tang, Jiawei Ouyang, Fang Xiong, Can Guo, Yanyan Tang, Yujuan Zhou, Qianjin Liao, Xu Wu, Hui Wang, Jianjun Yu, Yong Li, Xiaoling Li, Guiyuan Li, Zhaoyang Zeng, Yixin Tan, Wei Xiong
Summary: Single-cell RNA sequencing is a crucial technology in tumor research, revealing tumor cell heterogeneity, monitoring tumor development, and aiding in the classification of immune cells and drug resistance mechanisms.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Genetics & Heredity
Siyuan Chen, Weibo Jiang, Yanhui Du, Manshi Yang, Yihan Pan, Huan Li, Mengying Cui
Summary: Single-cell sequencing technology is revolutionizing our understanding of cellular components, functions, and interactions by avoiding heterogeneity-related noise. It can characterize multiple cell types and uncover the mechanisms of gene regulatory networks, transcription dynamics, and proteomic profiling.
FRONTIERS IN GENETICS
(2023)
Review
Oncology
Wenyue Yu, Chun Wang, Zhiqun Shang, Jing Tian
Summary: Single-cell RNA sequencing is an advanced technology that provides insights at the individual cell level and is particularly well-suited for identifying rare cell populations. In the context of prostate cancer, a disease characterized by significant heterogeneity and multiple stages of progression, single-cell RNA sequencing emerges as a powerful tool for uncovering its intricate secrets.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Guangxu Cao, Jiali Yue, Yetian Ruan, Ya Han, Yong Zhi, Jianqiao Lu, Min Liu, Xinxin Xu, Jin Wang, Quan Gu, Xuejun Wen, Jinli Gao, Qingfeng Zhang, Jiuhong Kang, Chenfei Wang, Fang Li
Summary: This study conducted single-cell RNA and TCR sequencing of cervical cancer tumors and adjacent normal tissues to map the immune landscape. The study revealed tumor-specific germinal center B cells associated with improved clinical outcomes and established a joint model of tumor and stromal cells to predict patients' prognosis. These findings provide information for future combinational immunotherapy.
Article
Immunology
Chunbo Li, Danyang Liu, Shimin Yang, Keqin Hua
Summary: This study used single-cell sequencing to investigate the molecular mechanism of lymph node metastasis in cervical cancer. The results showed that epithelial cells in metastatic lymph nodes were associated with cell cycle-related and immune response-related signaling pathways, while epithelial cells in primary tumors were enriched in epithelial-mesenchymal transition and oxidative phosphorylation. Additionally, metastatic lymph nodes exhibited an early activated tumor microenvironment with changes in the quantity of T cells and macrophages compared to normal lymph nodes. Cancer-associated fibroblasts in positive lymph nodes were associated with immune regulation, while fibroblasts in tumors underwent epithelial-mesenchymal transition.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Oncology
Si-Qing Liu, Zhi-Jie Gao, Juan Wu, Hong-Mei Zheng, Bei Li, Si Sun, Xiang-Yu Meng, Qi Wu
Summary: This study combines single-nucleus RNA sequencing and spatial transcriptomics to identify cell populations and their spatial distribution in breast cancer tissues. Malignant cells are clustered into distinct subpopulations with diverse features and functions, and their mapping in tissue regions shows differential enrichment of stromal cell types.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Article
Oncology
Vincenza Conteduca, Sheng-Yu Ku, Luisa Fernandez, Angel Dago-Rodriquez, Jerry Lee, Adam Jendrisak, Megan Slade, Cole Gilbertson, Jyothi Manohar, Michael Sigouros, Yipeng Wang, Ryan Dittamore, Rick Wenstrup, Juan Miguel Mosquera, Joseph D. Schonhoft, Himisha Beltran
Summary: Neuroendocrine prostate cancer is an aggressive variant of prostate cancer that often involves the combined loss of tumor suppressor genes like RB1, TP53, and PTEN. It is believed to arise from a trans-differentiation process from a precursor prostate adenocarcinoma during treatment resistance. Single-cell genomic analysis and phenotypic evaluation of cellular diversity can provide insights into tumor heterogeneity in advanced prostate cancer patients.
NPJ PRECISION ONCOLOGY
(2021)
Review
Immunology
Rongbo Wen, Leqi Zhou, Zhiying Peng, Hao Fan, Tianshuai Zhang, Hang Jia, Xianhua Gao, Liqiang Hao, Zheng Lou, Fuao Cao, Guanyu Yu, Wei Zhang
Summary: Colorectal Cancer (CRC) is a common gastrointestinal tumor with high heterogeneity. Traditional sequencing methods cannot capture the heterogeneity of individual cancer cells. Single-cell sequencing technology can analyze the genetic and protein differences between cells, providing a more comprehensive understanding of colorectal cancer development and metastasis. This study reviews the application of single-cell sequencing in analyzing the tumor microenvironment of CRC and exploring the mechanisms involved in metastasis and progression, providing a reference for potential treatment options.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Rulon Mayer, Charles B. Simone, Baris Turkbey, Peter Choyke
Summary: This study focused on the relationship among the prostate tumor's shape, volume, and potential aggressiveness. It found that prostate tumor eccentricity, determined by histology or MRI, more accurately predicted Gleason score than prostate tumor volume. Combining tumor eccentricity with volume from histology-based analysis enhanced Gleason score prediction, unlike MRI.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2022)
Review
Oncology
Jing Li, Nan Yu, Xin Li, Mengna Cui, Qie Guo
Summary: Tumorigenesis is the process of clonal dysplasia caused by various carcinogenic factors, leading to the collapse of normal growth regulation in cells. Tumor cells exhibit intratumor heterogeneity, which plays a crucial role in tumor development, metastasis, chemoresistance, and immune escape. Single-cell sequencing is a powerful tool that can help understand the diversity and complexity of the tumor microenvironment, improving cancer diagnosis, prognosis, and therapeutic response monitoring.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pathology
Chantal Atallah, Ants Toi, Theodorus H. van der Kwast
Summary: Several modifications have been made to the Gleason grading system, including the definition and reporting of GP5, with the addition of sub-patterns over time. The presence of GP5 indicates an unfavorable prognosis, but there is a lack of data on the prognostic impact of individual GP5 sub-patterns.
Article
Medicine, General & Internal
Michael Xiang, Ting Martin Ma, Ricky Savjani, Erqi L. Pollom, R. Jeffrey Karnes, Tristan Grogan, Jessica K. Wong, Giovanni Motterle, Jeffrey J. Tosoian, Bruce J. Trock, Eric A. Klein, Bradley J. Stish, Robert T. Dess, Daniel E. Spratt, Avinash Pilar, Chandana Reddy, Rebecca Levin-Epstein, Trude B. Wedde, Wolfgang A. Lilleby, Ryan Fiano, Gregory S. Merrick, Richard G. Stock, D. Jeffrey Demanes, Brian J. Moran, Hartwig Huland, Phuoc T. Tran, Santiago Martin, Rafael Martinez-Monge, Daniel J. Krauss, Eyad I. Abu-Isa, Ridwan Alam, Zeyad Schwen, Thomas M. Pisansky, C. Richard Choo, Daniel Y. Song, Stephen Greco, Curtiland Deville, Todd McNutt, Theodore L. DeWeese, Ashley E. Ross, Jay P. Ciezki, Paul C. Boutros, Nicholas G. Nickols, Prashant Bhat, David Shabsovich, Jesus E. Juarez, Natalie Chong, Patrick A. Kupelian, Matthew B. Rettig, Nicholas G. Zaorsky, Alejandro Berlin, Jonathan D. Tward, Brian J. Davis, Robert E. Reiter, Michael L. Steinberg, David Elashoff, Eric M. Horwitz, Rahul D. Tendulkar, Derya Tilki, Johannes Czernin, Andrei Gafita, Tahmineh Romero, Jeremie Calais, Amar U. Kishan
Summary: PSMA PET/CT can detect previously occult nonlocalized prostate cancer with important clinical implications. The upstaging probability on PSMA PET/CT is associated with long-term prognosis and shows superior risk discrimination compared with existing tools in high-risk patients.
Article
Medicine, Research & Experimental
Boda Guo, Shengjing Liu, Miao Wang, Huimin Hou, Ming Liu
Summary: Renal cell carcinoma with liver metastasis is associated with poor prognosis, however, nephrectomy can significantly prolong overall survival in eligible patients. The study conducted using the SEER database showed that factors such as age, histologic type, and metastasis locations were significant predictors of survival in these patients.
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2021)
Article
Urology & Nephrology
Miao Wang, Yi-feng Yang, Bo-da Guo, Hui-min Hou, Ling-feng Meng, Xuan Wang, Millicent Amankwah, Bao-qiong Liu, Cheng-yue Jin, Ming Liu, Jian-ye Wang
Summary: This study found that patients with atrial fibrillation (AF) undergoing radical prostatectomy (RP) had higher rates of postoperative cardiac complications, longer hospital stays, and higher costs. Particularly in laparoscopic RP and open RP, the risk of cardiac complications was higher in AF patients.
WORLD JOURNAL OF UROLOGY
(2021)
Letter
Urology & Nephrology
Boda Guo, Ming Liu
WORLD JOURNAL OF UROLOGY
(2021)
Editorial Material
Oncology
Boda Guo, Ming Liu
Letter
Urology & Nephrology
Boda Guo, Ming Liu
WORLD JOURNAL OF UROLOGY
(2022)
Letter
Urology & Nephrology
Boda Guo, Ming Liu
WORLD JOURNAL OF UROLOGY
(2022)
Letter
Urology & Nephrology
Boda Guo, Ming Liu
WORLD JOURNAL OF UROLOGY
(2022)
Letter
Andrology
Boda Guo, Ming Liu
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhengtong Lv, Cheng Pang, Jinfu Wang, Haoran Xia, Jingchao Liu, Qiuxia Yan, Shengjie Liu, Ming Liu, Jianye Wang
Summary: This study established an eight-immune-related gene risk model with strong predictive power for the prognosis and immunotherapeutic effect of bladder cancer patients. The model was associated with tumor characteristics, tumor type, immune cell infiltration, and gene mutation status. A nomogram was developed to effectively predict the long-term overall survival probability of these patients.
Letter
Urology & Nephrology
Boda Guo, Ming Liu
JOURNAL OF UROLOGY
(2021)
Article
Andrology
Miao Wang, Mingzi Yang, Pengjie Wu, Shumin Deng, Jianlong Wang, Jia Chen, Jianye Wang, Ming Liu
Summary: Primary urethral carcinoma (PUC) is a rare malignancy, and in this case report, a 75-year-old male patient with PUC involving both distal and proximal urethra underwent a transperineal-incision urethrectomy with laparoscopic prostatectomy and iliac lymphadenectomy, resulting in optimal outcomes. Histopathological analysis revealed a moderately-high differentiated PUC with no positive lymph nodes, and the patient had uneventful post-operative recovery with no recurrence of lesions detected on follow-up imaging.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Article
Oncology
Shicong Lai, Xingbo Long, Pengjie Wu, Jianyong Liu, Samuel Seery, Huimin Hou, Ming Liu, Yuan Li, Jianye Wang
Summary: Ki-67 expression levels were found to be associated with intravesical recurrence in upper tract urothelial carcinoma patients. The comprehensive model incorporating Ki-67 with traditional clinicopathological factors showed superior predictive accuracy compared to other models.
JAPANESE JOURNAL OF CLINICAL ONCOLOGY
(2021)
Letter
Urology & Nephrology
Boda Guo, Ming Liu
JOURNAL OF UROLOGY
(2021)
Letter
Oncology
Boda Guo, Ming Liu
JOURNAL OF THE NATIONAL COMPREHENSIVE CANCER NETWORK
(2021)