Article
Biochemistry & Molecular Biology
Shi-Zheng Jia, Xin-Wen Xu, Zhong-Hao Zhang, Chen Chen, Yu-Bin Chen, Shao-Ling Huang, Qiong Liu, Peter R. Hoffmann, Guo-Li Song
Summary: Selenoprotein K plays a critical role in regulating intracellular calcium flux, degradation pathways, and response to stress in neurons. Knockout of SELENOK in neurons leads to increased apoptosis, activated cascade pathways, and decreased cognitive ability and increased anxiety in older mice.
Article
Cell Biology
Haiying Liu, Linyu Dai, Ming Wang, Fumin Feng, Yonghong Xiao
Summary: This study demonstrated that tunicamycin can induce the apoptosis of HSCs by increasing intracellular Ca2+ concentration and enhancing the expression of ERS proteins, while decreasing the expression of antiapoptotic proteins. The findings suggest that tunicamycin induces HSC apoptosis through the calpain-2/Ca2+-dependent ERS pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Pengcheng Xing, Xiang Li, Yiwei Bai, Zhihui Jiao
Summary: With the increased use of pesticides and antibiotics in agriculture, the pollution of surrounding runoff has worsened, posing risks to non-target species such as fish. Cypermethrin and sulfamethoxazole have been found to induce oxidative stress and endoplasmic reticulum stress, leading to toxic effects on cells. This study investigated the damage caused by these poisons to grass carp and their effect on the PERK/eif2 alpha/CHOP pathway. The results showed that the combined effect of cypermethrin and sulfamethoxazole was more severe than that of a single poison, indicating that the damage to grass carp myocardium tissue would worsen with the presence of these poisons.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Immunology
Jiang Zhu, Xia Zhang, Hong Xie, Yuye Wang, Xiaoxiao Zhang, Zhaoheng Lin
Summary: This study reveals that the binding of cardiomyocyte STIM1 to GRP78 alleviates Dox cardiotoxicity by inhibiting pro-apoptotic ER stress.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Jialin Li, Kan Chen, Jianhua Huang, Dongqing Chu, Miaomiao Tian, Keqiang Huang, Chunyu Ma
Summary: Asiatic acid (AA) is shown to induce apoptotic death in tongue cancer cells in vitro and in vivo, potentially through mechanisms involving increased calcium levels and endoplasmic reticulum stress. This suggests that AA could be a promising therapeutic tool for tongue cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Kang Yang, Ruixue Xu, Weidong Le
Summary: This study investigated the antineoplastic effect and pathways of action of dopamine receptor D1 agonist SKF83959 on GBM cells. The results showed that SKF83959 induced apoptotic cell death in GBM cells through mechanisms such as increasing intracellular calcium levels and oxidative stress. These findings suggest a potential therapeutic target for human GBM treatment by regulating calpain expression and activity.
Article
Pharmacology & Pharmacy
Hussam Albassam, Daniel A. Ladin, Ahmed Elhassanny, Colin Burns, Rukiyah Van Dross-Anderson
Summary: Research demonstrates that 15d-PMJ(2) induces cancer cell apoptosis by stimulating the CHOP10/TRB3/Akt inhibition pathway. Deletion of CHOP10 reduces the expression of DR5, ERO1 alpha, and TRB3.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Shiqi Tang, Xueqin Wu, Qing Dai, Zhi Li, Shikun Yang, Yan Liu, Bin Yi, Jianwen Wang, Qin Liao, Wei Zhang, Hao Zhang
Summary: Activating transcription factor 4 (ATF4) is a crucial mediator of endoplasmic reticulum stress (ERS) and plays a significant role in acute kidney disease (AKI). Vitamin D receptor (VDR) has been shown to provide renoprotection in rodent models of AKI. This study aimed to investigate the involvement of ATF4 and ERS in the protective effect of VDR against ischemia-reperfusion (I/R) induced AKI. The results demonstrated that VDR agonist paricalcitol and VDR overexpression reduced renal injury and apoptosis by decreasing ATF4 expression and attenuating ERS. Conversely, VDR deletion resulted in increased ATF4, exacerbated ERS, and worsened renal injury in I/R mice models. Furthermore, ATF4 partially abolished the protective effect of paricalcitol against ERS and apoptosis induced by Tunicamycin, while inhibition of ATF4 enhanced the protective effect of paricalcitol. Bioinformatics analysis and experimental assays confirmed the potential binding sites of VDR on the ATF4 promoter sequence. In conclusion, VDR attenuates I/R-induced AKI by suppressing ERS through transcriptional regulation of ATF4.
CELL DEATH DISCOVERY
(2023)
Article
Pharmacology & Pharmacy
Zhiqiang Qian, Ling Zhu, Yeli Li, Yiqi Li, Yuting Wu, Shu Fu, Danli Yang
Summary: The study found that ICA can alleviate hypertension-induced cardiomyocyte damage by inhibiting ERS. In the SHR group, high blood pressure, decreased left ventricular function, cardiomyocyte apoptosis, and increased ERS-related protein expression were observed, while ICA treatment reduced blood pressure and improved cardiac function.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Jing Wang, Ru Wang, Jiali Li, Zhuhua Yao
Summary: Rutin can alleviate myocardial injury induced by high glucose through inhibition of apoptosis and endoplasmic reticulum stress in H9C2 cardiac cells.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Environmental Sciences
Jicang Wang, Lulu Ding, Ke Wang, Ruxue Huang, Wenjing Yu, Bingzhao Yan, Hongwei Wang, Cai Zhang, Zijun Yang, Zongping Liu
Summary: This study aimed to determine the protective effect of quercetin (Que) on cadmium (Cd)-induced hepatotoxicity. Through in vivo and in vitro experiments using rat models, the researchers found that Cd caused liver injury and cell apoptosis, and activated endoplasmic reticulum stress (ERS) signaling pathways. However, Que was able to improve hepatocyte vitality, reduce liver damage and oxidative stress caused by Cd.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yiqi Wang, Lian Duan, Xing Zhang, Yang Jiao, Ying Liu, Lingling Dai, Hong Yan
Summary: The study demonstrated that chronic exposure to ACR can impair motor skills, muscle strength, and coordination in rats, as well as damage the morphology of the cerebellum, leading to increased apoptosis and initiation of autophagy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Agriculture, Dairy & Animal Science
Xiaocheng Huang, Shanghong Ji, Chenchen Bian, Jian Sun, Hong Ji
Summary: This study revealed that DHA induces adipocyte apoptosis by activating endoplasmic reticulum stress and unfolded protein response. BOK is identified as a key linker between ER stress and apoptosis, and it participates in DHA-induced apoptosis through the ATF6 and IRE1α signaling pathways.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Shreya Das, Arunima Mondal, Chandrani Dey, Santanu Chakraborty, Rudranil Bhowmik, Sanmoy Karmakar, Arunima Sengupta
Summary: In the mammalian heart, fetal cardiomyocytes proliferate prior to birth and adult cardiomyocytes re-enter the cell cycle postinjury to promote cardiac regeneration. Disruption of the endoplasmic reticulum (ER) machinery leads to ER stress, which has a fine balance between protective and proapoptotic responses. T-box transcription factor 20 (Tbx20) is upregulated in ER stress and promotes cardiomyocyte proliferation and limits apoptosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Bin-Bin Zhu, Zhi-Cheng Zhang, Jian Li, Xing-Xing Gao, Yuan-Hua Chen, Hua Wang, Lan Gao, De-Xiang Xu
Summary: High doses of DEHP exposure during puberty induce germ cell apoptosis through initiating endoplasmic reticulum stress in testes. The apoptosis of germ cells and the changes in related protein levels occur after exposure to DEHP.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Critical Care Medicine
Yuhu He, Caojian Zuo, Daile Jia, Peiyuan Bai, Deping Kong, Di Chen, Guizhu Liu, Juanjuan Li, Yuanyang Wang, Guilin Chen, Shuai Yan, Bing Xiao, Jian Zhang, Linjuan Piao, Yanli Li, Yi Deng, Bin Li, Philippe P. Roux, Katrin Andreasson, Rich Breyer, Yunchao Su, Jian Wang, Ankang Lyu, Yujun Shen, Ying Yu
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Article
Cell Biology
Yuxin Wang, Qiaoling Song, Wei Huang, Yuxi Lin, Xin Wang, Chenyao Wang, Belinda Willard, Chenyang Zhao, Jing Nan, Elise Holvey-Bates, Zhuoya Wang, Derek Taylor, Jinbo Yang, George R. Stark
Summary: Type I interferons (IFN-I) play a crucial role in protecting against viral infections, with STAT2 being a key component in driving gene expression in response to IFN-I. The phosphorylation of STAT2 at T404 acts as a critical conformational switch, disrupting an inactive STAT1-STAT2 dimer and enhancing the antiviral defense response to IFN-I. Additionally, virus infection-induced activation of IKK-epsilon can directly phosphorylate T404, further facilitating the antiviral defense mechanism.
Article
Cardiac & Cardiovascular Systems
Daile Jia, Peiyuan Bai, Naifu Wan, Jiao Liu, Qian Zhu, Yuhu He, Guilin Chen, Jing Wang, Han Chen, Chen Wang, Ankang Lyu, Michael Lazarus, Yunchao Su, Yoshihiro Urade, Ying Yu, Jian Zhang, Yujun Shen
CIRCULATION RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Chenyao Wang, Xin Wang, Manoj Veleeparambil, Patricia M. Kessler, Belinda Willard, Saurabh Chattopadhyay, Ganes C. Sen
Article
Biochemistry & Molecular Biology
Huiwen Ren, Shengkai Zuo, Yayan Hou, Wenlong Shang, Na Liu, Zhuming Yin
Article
Medicine, Research & Experimental
Shuai Yan, Manju Kumari, Haopeng Xiao, Christopher Jacobs, Shihab Kochumon, Mark Jedrychowski, Edward Chouchani, Rasheed Ahmad, Evan D. Rosen
Summary: This study showed that the innate immune transcription factor IRF3 can inhibit thermogenic gene expression in adipocytes, potentially by driving ISG15 expression and reducing glycolytic enzyme function. Mice lacking ISG15 exhibited similar characteristics to mice lacking IRF3 in adipocytes, both showing increased energy expenditure and resistance to diet-induced obesity.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Jiao Liu, Yuxuan Jin, Bei Wang, Yiran Wang, Shengkai Zuo, Jinying Zhang
Summary: The study found that the activation of dopamine D1 receptor can reduce cardiac inflammation damage induced by DOX by inhibiting the NLRP3 inflammasome. Additionally, DRD1 signaling downregulates the NLRP3 inflammasome through cAMP and reduces cardiac injury and fibrosis in a DOX-treated mouse model.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Shengkai Zuo, Bei Wang, Jiao Liu, Deping Kong, Hui Cui, Yaonan Jia, Chenyao Wang, Xin Xu, Guilin Chen, Yuanyang Wang, Linlin Yang, Kai Zhang, Ding Ai, Jie Du, Yujun Shen, Ying Yu
Summary: The study discovered a novel anti-fibrotic function of CRTH2 in the endoplasmic reticulum membrane, where it interacts with LARP6 to promote degradation of collagen mRNA, thereby inhibiting collagen biosynthesis in fibroblasts and reducing organ fibrosis.
Article
Biochemistry & Molecular Biology
Jiao Liu, Yuxuan Jin, Bei Wang, Jinying Zhang, Shengkai Zuo
Summary: The study found that C188-9, a small-molecule inhibitor of STAT3, has anti-fibrotic effects both in vitro and in vivo. It can reduce CF activation and fibrotic gene expression induced by transforming growth factor-beta 1, and alleviate heart injury and fibrosis in a mouse model by suppressing STAT3 phosphorylation and activation.
Article
Microbiology
Chenyao Wang, Nikhil Sharma, Manoj Veleeparambil, Patricia M. Kessler, Belinda Willard, Ganes C. Sen
Summary: The nature and intensity of the innate immune response to virus infection are critical for the course of pathogenesis in the host. STING, an endoplasmic reticulum-associated protein, plays a key role in triggering responses to microbial or cellular cytoplasmic DNA. Phosphorylation of Tyr240 of STING by the tyrosine kinase Syk is essential for all signaling activities of STING, and its signaling is necessary for interferon induction.
Article
Microbiology
Nikhil Sharma, Chenyao Wang, Patricia Kessler, Ganes C. Sen
Summary: This study reveals that HSV1 evades antiviral immune response by inducing miR-24, which inhibits STING signaling. However, intervention targeting miR-24 to enhance STING's antiviral action can effectively inhibit HSV1 replication in host cells.
Article
Immunology
Xixi Tao, Rui Zhang, Ronglu Du, Tingting Yu, Hui Yang, Jiwen Li, Yuhong Wang, Qian Liu, Shengkai Zuo, Xi Wang, Michael Lazarus, Lu Zhou, Bangmao Wang, Ying Yu, Yujun Shen
Summary: EP3 is required for the adhesion and cytotoxicity of NK cells toward HSCs and may serve as a therapeutic target for the management of LF.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Cell Biology
Suraj J. Patel, Nan Liu, Sam Piaker, Anton Gulko, Maynara L. Andrade, Frankie D. Heyward, Tyler Sermersheim, Nufar Edinger, Harini Srinivasan, Margo P. Emont, Gregory P. Westcott, Jay Luther, Raymond T. Chung, Shuai Yan, Manju Kumari, Reeby Thomas, Yann Deleye, Andre Tchernof, Phillip J. White, Guido A. Baselli, Marica Meroni, Dario F. De Jesus, Rasheed Ahmad, Rohit N. Kulkarni, Luca Valenti, Linus Tsai, Evan D. Rosen
Summary: This study reveals the important role of hepatic interferon regulatory factor 3 (IRF3) in regulating glucose homeostasis through the transcription factor Ppp2r1b. Elimination or reduction of IRF3 protects against steatosis and dysglycemia in obese mice. The study also suggests that obesity-induced insulin resistance and the restoration of glucose homeostasis can be reversed by inhibiting IRF3 expression. In addition, the activation of liver IRF3 in obese humans is enhanced and can be improved after bariatric surgery.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Xixi Tao, Ronglu Du, Shumin Guo, Xiangling Feng, Tingting Yu, Qian OuYang, Qiaoli Chen, Xutong Fan, Xueqi Wang, Chen Guo, Xiaozhou Li, Fengxia Xue, Shuai Chen, Minghan Tong, Michael Lazarus, Shengkai Zuo, Ying Yu, Yujun Shen
Summary: The PGE(2)-EP3 axis plays a protective role in interscapular BAT development by stabilizing WTAP/Zfp410 signaling.
Article
Multidisciplinary Sciences
Chenyao Wang, Jing Nan, Elise Holvey-Bates, Xing Chen, Samantha Wightman, Muhammad-Bilal Latif, Junjie Zhao, Xiaoxia Li, Ganes C. Sen, George R. Stark, Yuxin Wang
Summary: In cancer cells, the abnormal presence of DNA in the cytoplasm activates cGAS and STING. STAT2 inhibits the expression of IRF3-dependent genes induced by cGAMP by binding to STING and blocking its intracellular trafficking. STAT2 reshapes STING signaling by inhibiting IRF3-dependent gene induction, but not NF-xB-dependent gene induction. This noncanonical activity of STAT2 depends on the phosphorylation of threonine 404, which promotes the formation of a STAT2:STING complex that keeps STING bound to the endoplasmic reticulum (ER) and increases resistance to DNA damage.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)