Article
Biochemistry & Molecular Biology
Nikoloz Nioradze, Dmitri Ciornii, Adrian Kolsch, Gero Gobel, Dimitri E. Khoshtariya, Athina Zouni, Fred Lisdat
Summary: In this study, biohybrid electrodes were developed by combining 3D indium tin oxide (ITO) with photosystem I and cytochrome c. The thickness of the 3D ITO layers was controlled by varying the electrospinning time to enhance the photocurrent. Performance parameters of the electrode were analyzed under different illumination intensities.
BIOELECTROCHEMISTRY
(2021)
Article
Engineering, Electrical & Electronic
Akanksha Singh, Sarthak Mandal, Shaojiang Chen, Minghui Liu, Christopher J. Gisriel, Anne-Marie Carey, Hao Yan, Dong-Kyun Seo, Su Lin, Neal W. Woodbury
Summary: A biohybrid electronic system has been developed with a photosystem I (PSI) fixed on a porous antimony-doped tin oxide electrode, resulting in light-powered reduction of NADPH. Cytochrome c was used to orient PSI reaction centers and transport electrons, generating a photocurrent. Despite attempts to catalyze dye reduction by NADPH, dye reduction occurred even without enzymes, with direct light-dependent reduction of NADPH and subsequent reduction of resazurin.
ACS APPLIED ELECTRONIC MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Tom Schwartz, Mariia Fadeeva, Daniel Klaiman, Nathan Nelson
Summary: The mutant TSP9 of Chlamydomonas reinhardtii, isolated through negative selection of UV irradiation-mutated cells, has a single amino acid mutation in the Rieske protein. It loses its function at 37 degrees C but recovers at 25 degrees C. The mutant showed structural alterations in photosystem I and II supercomplexes, suggesting a possible response mechanism involving the exclusion of certain subunits.
Article
Chemistry, Multidisciplinary
Roman A. Voloshin, Ekaterina S. Lokteva, Suleyman I. Allakhverdiev
Summary: Biohybrid devices based on natural pigment-protein complexes are being widely studied, with a focus on designing electrodes using photo-system I. This review emphasizes the benefits of nanostructured electrodes, recombinant photosystems, and electrophoretic deposition techniques for uniform photosystem I orientation on an electrode.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Plant Sciences
Long Than, Kody D. Wolfe, David E. Cliffel, G. Kane Jennings
Summary: One of the main challenges in developing efficient Photosystem I-based biohybrid solar cells is the lack of an electrochemical pathway for efficient electron transfer. This study utilizes cytochrome c as an electron donor to the P-700 site in Photosystem I, mimicking nature's inspiration. The researchers use a vacuum-assisted drop-casting technique to create a Photosystem I/cytochrome c composite film, demonstrating improved cathodic photocurrent due to enhanced electron transfer rate within the film.
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Chemistry, Physical
Andrey Zaspa, Liya Vitukhnovskaya, Aida Mamedova, Suleyman I. Allakhverdiev, Alexey Semenov, Mahir Mamedov
Summary: This study directly measured the long-term photovoltage generation by the photosynthetic pigment-protein complex of photosystem I (PS I) from cyanobacterium Synechocystis sp. PCC 6803. By immobilizing PS I onto a membrane filter and sandwiching it between two semiconductor electrodes, a stable photovoltage system was successfully developed. The results demonstrated that the system could generate a stable voltage signal under certain conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Chemistry, Multidisciplinary
Sudipta Bera, Sharada Govinda, Jerry A. Fereiro, Israel Pecht, Mordechai Sheves, David Cahen
Summary: In this study, the electron transport efficiency of solid-state protein-mediated junctions was investigated using streptavidin (STV). The results showed that there was no statistically significant difference in conductivity between free STV and STV bound to biotin, despite some structural changes caused by biotin binding.
Review
Plant Sciences
Carmen Castell, Luis A. Rodriguez-Lumbreras, Manuel Hervas, Juan Fernandez-Recio, Jose A. Navarro
Summary: The text discusses the evolution and functional differences of the copper-protein plastocyanin (Pc) among different groups of organisms, as well as the mechanisms of electron transfer in the green, red, and diatom lineages.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
H. Doerpholz, S. Subramanian, A. Zouni, F. Lisdat
Summary: In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus were prepared and characterized. The direct electron transfer between PSI-Fd and the electrode was investigated. The results showed that the modified PSI-Fd photobioelectrode had a lower photocurrent and a lower onset potential of the cathodic photocurrent compared to native PSI, mainly due to the different adsorption behavior of the PSI-Fd construct onto the 3D ITO electrode. However, the overall behavior was similar to PSI. First experiments demonstrated the usage of the PSI-Fd photobioelectrode for enzyme-driven NADPH generation.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jihyun Kim, J. Tassilo Grun, Mihajlo Novakovic, Eriks Kupce, Rina Rosenzweig, Lucio Frydman
Summary: This paper discusses a new cross polarization (CP) strategy that combines frequency-swept and phase-modulated pulses to improve the efficiency of N-15 transfers. Theoretical analysis and experimental results demonstrate that this new strategy outperforms current options on urea, amino acids, and intrinsically disordered proteins.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemistry & Molecular Biology
Antonio Ranieri, Marco Borsari, Stefano Casalini, Giulia Di Rocco, Marco Sola, Carlo Augusto Bortolotti, Gianantonio Battistuzzi
Summary: Cytochrome c is a small globular protein that plays a crucial role in electron transfer within mitochondria, but it can also exhibit non-native catalytic activity and be used in the development of biosensors. Through point mutations or controlled unfolding, its functionality can be transformed, offering potential for optimizing biosensors and addressing related issues.
Article
Chemistry, Multidisciplinary
Yue Zeng, Xin Zhou, Ruilian Qi, Nan Dai, Xuancheng Fu, Hao Zhao, Ke Peng, Haitao Yuan, Yiming Huang, Fengting Lv, Libing Liu, Shu Wang
Summary: This study achieved promising results by constructing a hybrid biosystem combining organic material with cyanobacteria to enhance photosynthesis efficiency and regulate protein redox state effectively.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Diego Gauto, Ons Dakhlaoui, Ildefonso Marin-Montesinos, Sabine Hediger, Gael De Paepe
Summary: High-field dynamic nuclear polarization is expanding the applications of solid-state NMR in surface chemistry, materials science, and structural biology. Targeted DNP approaches, where paramagnets are specifically located on biomolecular systems, show potential for improving NMR sensitivity without the need for glass-forming DNP matrices. This technique is particularly useful for studying diluted biomolecular systems like membrane proteins in their lipidic environment and extracting structural information through paramagnetic relaxation enhancement.
Article
Multidisciplinary Sciences
Florencia Tomasina, Jennyfer Martinez, Ari Zeida, Maria Laura Chiribao, Veronica Demicheli, Agustin Correa, Celia Quijano, Laura Castro, Robert H. Carnahan, Paige Vinson, Matt Goff, Tracy Cooper, W. Hayes McDonald, Natalie Castellana, Luciana Hannibal, Paul T. Morse, Junmei Wan, Maik Huttemann, Ronald Jemmerson, Lucia Piacenza, Rafael Radi
Summary: Cytochrome c (cyt c) undergoes reversible conformational changes under biologically relevant conditions, but revealing these alternative conformations at the cell and tissue level is challenging. This study successfully resurrected a monoclonal antibody (mAb) that recognizes a key conformational change in cyt c and showed similar performance to the original mAb. Using this antibody, alternative conformations of cyt c were identified in cells under oxidative or senescence-induced challenge, and these conformations translocated to the nucleus without causing apoptosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Physical
Panpan Wang, Fangyuan Zhao, Anna Frank, Sarra Zerria, Anna Lielpetere, Adrian Ruff, Marc M. Nowaczyk, Wolfgang Schuhmann, Felipe Conzuelo
Summary: Photosystem I (PSI) has the potential for fabrication of light-driven semi-artificial bioelectrodes, with Langmuir monolayers and specially designed redox polymers enabling efficient electron transfer for advanced biophotoanodes. The developed bioelectrode can generate substantial photocurrents and even operate at low potentials, surpassing the performance of comparable devices. When combined with a gas-breathing biocathode, the assembly forms a biophotovoltaic cell that delivers considerable power outputs, showcasing the potential for advanced biophotovoltaic device development.
ADVANCED ENERGY MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Elisabetta Mileo, Marianne Ilbert, Alessandro Barducci, Patricia Bordes, Marie-Pierre Castanie-Cornet, Cyrille Garnier, Pierre Genevaux, Reynald Gillet, Pierre Goloubinoff, Francoise Ochsenbein, Gilbert Richarme, Chantal Iobbi-Nivol, Marie-Therese Giudici-Orticoni, Brigitte Gontero, Olivier Genest
Article
Chemistry, Multidisciplinary
X. Wang, M. Roger, R. Clement, S. Lecomte, F. Biaso, L. A. Abriata, P. Mansuelle, I. Mazurenko, M. T. Giudici-Orticoni, E. Lojou, M. Ilbert
Article
Chemistry, Medicinal
Ana Carina Fernandes, Mengjiao Liu, Teresa Sorbo, Lia Christina Appold, Marianne Ilbert, Geraldine Ferracci, Fabian Kiessling, Ricardo J. F Branco, Twan Lammers, Olga Iranzo
FUTURE MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Physical
Vivek Pratap Hitaishi, Ievgen Mazurenko, Malek Harb, Romain Clement, Marion Taris, Sabine Castano, David Duche, Sophie Lecomte, Marianne Ilbert, Anne de Poulpiquet, Elisabeth Lojou
Article
Chemistry, Multidisciplinary
Xie Wang, Romain Clement, Magali Roger, Marielle Bauzan, Ievgen Mazurenko, Anne de Poulpiquet, Marianne Ilbert, Elisabeth Lojou
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Vivek Pratap Hitaishi, Romain Clement, Ludovica Quattrocchi, Philippe Parent, David Duche, Lisa Zuily, Marianne Ilbert, Elisabeth Lojou, Ievgen Mazurenko
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemistry & Molecular Biology
Romain Clement, Xie Wang, Frederic Biaso, Marianne Ilbert, Ievgen Mazurenko, Elisabeth Lojou
Summary: Mutations in key amino acid residues in the first and second coordination spheres of T1 Cu lead to unexpected influences on enzymatic activity, indicating that the methionine rich domain is not the sole factor in the cuprous oxidase activity of CueO-like enzymes.
Review
Biochemistry & Molecular Biology
Rosi Fassler, Lisa Zuily, Nora Lahrach, Marianne Ilbert, Dana Reichmann
Summary: Bacteria adapt to changing environments by utilizing protein switches to modulate their behavior and combat oxidative stress, with thiol switches and metal centers being primary targets of ROS. Cells use these reactive sites as sensors to detect and fight oxidative stress conditions, inducing gene expression for antioxidant strategies to protect the proteome against stress.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Microbiology
Lisa Zuily, Nora Lahrach, Rosi Fassler, Olivier Genest, Peter Faller, Olivier Seneque, Yann Denis, Marie-Pierre Castanie-Cornet, Pierre Genevaux, Ursula Jakob, Dana Reichmann, Marie-Therese Giudici-Orticoni, Marianne Ilbert
Summary: With the increase of antibiotic drug resistance, alternative antibacterial treatment strategies are needed. This study found that copper treatment causes protein aggregation, and bacteria rely on molecular chaperone systems to protect themselves from copper-induced protein aggregation.
Review
Chemistry, Physical
I. Mazurenko, T. Adachi, B. Ezraty, M. Ilbert, K. Sowa, E. Lojou
Summary: Multicopper oxidases are a widely studied family of enzymes involved in catalyzing the oxygen reduction reaction. This paper focuses on the less studied class of copper efflux oxidases, discussing the role of the methionine-rich domain in the electron transfer process and its implications in therapeutic applications.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Marie Corteggiani, Nadege Bossuet-Greif, Jean-Philippe Nougayrede, Deborah Byrne, Marianne Ilbert, Sebastien Dementin, Marie-Therese Giudici-Orticoni, Vincent Mejean, Eric Oswald, Olivier Genest
Summary: Chaperone proteins, such as Hsp90, play a critical role in maintaining protein homeostasis in all living cells. Recent studies have shown that bacterial Hsp90 is essential for the survival of bacteria under stress conditions and is involved in the virulence of pathogenic bacteria. In this study, the researchers used site-directed mutagenesis to disrupt the binding between Hsp90 and the chaperone protein DnaK, and found that this collaboration is crucial for the folding of client proteins in bacteria. They also demonstrated that this collaboration between Hsp90 and DnaK in bacteria has evolved to form a more complex collaboration between Hsp70 and Hsp90 in eukaryotes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Lana Weber, Atar Gilat, Nathanael Maillot, Deborah Byrne, Pascal Arnoux, Marie-Therese Giudici-Orticoni, Vincent Mejean, Marianne Ilbert, Olivier Genest, Rina Rosenzweig, Sebastien Dementin
Summary: Bacterial genomes contain genes that encode J-domain protein co-chaperones, which are important for recruiting the molecular chaperone DnaK to assist in the survival, adaptation, or fitness of the bacteria. The atc operon in the bacterium Shewanella oneidensis encodes several proteins, including AtcJ, AtcA, AtcB, and AtcC, which are all required for growth at low temperatures. AtcJ interacts with both DnaK and AtcC, and this interaction is critical for cold growth.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Solene G. Beauvois, Nicolas Flaugnatti, Marianne Ilbert, Marie Boyer, Esther Gavello-Fernandez, Remi Fronzes, Dukas Jurenas, Laure Journet
Summary: Bacteria compete for ecological niches and use T6SS to inject toxic effectors into target cells. PAAR protein plays a critical role in T6SS, interacting with VgrG protein to promote its structure formation and being necessary for T6SS baseplate assembly and sheath polymerization.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Enrico Falcone, Francesco Stellato, Bertrand Vileno, Merwan Bouraguba, Vincent Lebrun, Marianne Ilbert, Silvia Morante, Peter Faller
Summary: Copper is essential for most organisms, but excess copper can be toxic through various mechanisms. In this study, the reactivity of copper with a mixture of physiological reducing agents was investigated. The results showed that copper preferentially reacts with glutathione (GSH) and cysteine (Cys), halting the oxidation of ascorbate (AscH) and the release of reactive oxygen species (ROS). The formation of copper-thiolate clusters with GSH and Cys was observed, and a synergistic effect of Cys on copper toxicity was identified.