Article
Gastroenterology & Hepatology
Huabo Wang, Jie Lu, Jordan A. Mandel, Weiqi Zhang, Marie Schwalbe, Joanna Gorka, Ying Liu, Brady Marburger, Jinglin Wang, Sarangarajan Ranganathan, Edward Prochownik
Summary: The study found that the mutations of NFE2L2 and key transcripts, including Serpin E1, directly contribute to the specific features of hepatoblastoma.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Cell Biology
Kun Gao, Qing Shi, Yajuan Liu, Chenji Wang
Summary: This study reveals a negative regulatory role of SPOP in autophagy and NFE2L2 pathway activation. The study found that SPOP induces non-degradative ubiquitination of SQSTM1, thereby suppressing autophagy and NFE2L2 pathway activation. The PCa-associated SPOP mutants lose the capacity to ubiquitinate SQSTM1 and instead enhance autophagy and the antioxidant response in a dominant-negative manner.
Article
Cell Biology
Jian Li, Mouli Tian, Tong Hua, Haowei Wang, Mei Yang, Wenqian Li, Xiaoping Zhang, Hongbin Yuan
Summary: The impaired autophagy flux mainly occurs in astrocytes during the maintenance of neuropathic pain. Activation of autophagy can relieve pain, while inhibition of autophagy can aggravate pain and alter levels of neuroinflammation and reactive oxygen species. Further study shows that inhibition of autophagy can slow the induction of neuroinflammatory responses but increase their maintenance.
Article
Cell Biology
Arindam Bhattacharjee, Adel Urmosi, Andras Jipa, Levente Kovacs, Peter Deak, Aron Szabo, Gabor Juhasz
Summary: Using a Drosophila model, researchers show that disrupting ubiquitinated protein autophagy and forming polyubiquitinated aggregates has little effect on overall autophagy, proteasome activity, and fly healthspan. However, they find that the accumulation of ref(2)P/SQSTM1 activates the antioxidant pathway, resulting in increased tolerance to oxidative stress in mutant flies.
Article
Biochemistry & Molecular Biology
Tiantong Qiu, Xin Chen, Peng Xiao, Liqiang Wang, Wenhua Li
Summary: In this study, the potential toxicity and underlying mechanisms of FLU on aquatic organisms were investigated using zebrafish embryos as a model organism. The results showed that FLU can cause microphthalmia in zebrafish embryos by inducing retinal cell apoptosis, indicating its developmental toxicity.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Oncology
Wolfram C. M. Dempke, Martin Reck
Summary: NRF2 is the master regulator of cellular antioxidant responses and dysregulation of the KEAP1/NRF2 axis is associated with various diseases. Oxidative stress plays a crucial role in chronic diseases, particularly in NSCLC patients.
Article
Environmental Sciences
Ruiqi Fan, Wanjun Zhang, Lizhong Li, Li Jia, Jun Zhao, Zengming Zhao, Shuangqing Peng, Xiaoyan Yuan, Yiqiang Chen
Summary: The fungicide carbendazim and insecticide chlorpyrifos, used together to protect fruit and vegetable crops in China, have potentially harmful effects on zebrafish embryonic development, both individually and in combination, showing significant synergistic effects. These findings suggest the need for health and environmental risk assessments for various combinations of these agents.
Article
Environmental Sciences
Nikola Mitovic, Stefan Maksimovic, Darko Puflovic, Sanjin Kovacevic, Srdjan Lopicic, Jasna Todorovic, Svetolik Spasic, Marko Dincic, Jelena Nesovic Ostojic
Summary: Research shows that exposure to cadmium in early zebrafish development leads to hatching delays and morphological abnormalities, indicating significant effects of toxic metals on embryological processes.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Article
Marine & Freshwater Biology
Julian Navarrete, Peyton Wilson, Nicholas Allsing, Chandi Gordon, Rachel Margolis, Ashley V. Schwartz, Christine Cho, Brynn Rogowski, Jennifer Topps, Uduak Z. George, Karilyn E. Sant
Summary: Tris(4-chlorophenyl)methanol (TCPMOH) is a water contaminant believed to be a byproduct of DDT manufacturing, posing risks to marine species and human infants. Developmental exposure to TCPMOH in zebrafish embryos resulted in concentration-dependent increase in mortality and morphological deformities, along with alterations in gene expression and metabolic pathways. Overall, the study demonstrates that TCPMOH exhibits acute toxicity to zebrafish embryos at higher concentrations.
AQUATIC TOXICOLOGY
(2021)
Article
Engineering, Biomedical
Anand G. Vaish, Yuji Tomizawa, David F. Daggett, Kazunori Hoshino
Summary: This study proposes a new tool to track changes in the pressure exerted by the heart on the neighboring yolk during early cardiac growth using high-resolution optical elastography and stiffness measurements of surrounding tissues. The researchers found significant differences in yolk indentation pressure between wildtype fish and those treated with MS-222, a fish anesthetic that decreases cardiac contractility. The study allows for contractility and pressure evaluation at early developmental stages and could contribute to a better understanding of heart development and improved diagnostic tools for congenital heart disease.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Review
Pharmacology & Pharmacy
Alexandre Androuin, Frederik J. Verweij, Guillaume van Niel
Summary: Extracellular Vesicles (EVs) released during various pathophysiological processes can propagate through biological fluids, target cells, deliver content, and elicit functional responses, offering potential as biomarkers and drug nano-vehicles. Advances in imaging EVs in vivo, particularly in zebrafish embryos, provide insights into EVs dynamics, biodistribution, and functions to enhance their development for therapeutic applications.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Khoa Dang Dang, Chi Nguyen Quynh Ho, Huy Duc Van, Son Thanh Dinh, Quynh Thi Truc Nguyen, Tram Thi Thuy Nguyen, Xuyen Thi Ngoc Kien, Tuyet Van Dao, Hung Viet Nong, Minh Thai Nguyen, Chung Chinh Doan, Son Nghia Hoang, Thao Thi Phuong Nguyen, Long Thanh Le
Summary: The study aimed to evaluate the impact of hexavalent chromium on the development of zebrafish embryos. The embryos were exposed to different concentrations of chromium, and their survival rate, heart rate, and body length were measured. The expression of apoptosis- and antioxidant-related genes was analyzed using real-time RT-PCR and Western blot. The results showed that higher chromium concentrations led to decreased survival rate and body length, as well as increased heart rate in zebrafish larvae. The expression of cell-cycle-related and antioxidant-related genes was downregulated, while caspase 3 and Bax were upregulated, suggesting that hexavalent chromium altered zebrafish embryo development by affecting apoptosis- and antioxidant-related genes.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Marine & Freshwater Biology
Emmanolia Sergi, Michail Orfanakis, Anastasia Dimitriadi, Maria Christou, Anthi Zachopoulou, Chara Kourkouta, Alice Printzi, Sevasti-Kiriaki Zervou, Pavlos Makridis, Anastasia Hiskia, George Koumoundouros
Summary: This study examined the effects of exposure to Microcystis aeruginosa extract during the embryonic period on swimming performance and body development in fish. The results showed significant changes in swimming performance, cardiac shape, and vertebral abnormalities in fish exposed to the extract. These findings have important implications for wild fish populations.
AQUATIC TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Siti Nur Sharmila Abdullah, Kalai Arasu Subramaniam, Zahir Haizat Muhamad Zamani, Seri Narti Edayu Sarchio, Faizah Md Yasin, Suhaili Shamsi
Summary: This study investigated the toxicity effects of a nanoformulation of Curcumin (NanoCUR) on the embryonic development of zebrafish. It was found that NanoCUR exhibited improved toxicity profile compared to native CUR, with delayed toxicity response and reduced generation of reactive oxygen species (ROS). These findings highlight the potential of NanoCUR for clinical applications.
Article
Biochemistry & Molecular Biology
Samera H. Hamad, Stephanie A. Montgomery, Jeremy M. Simon, Brittany M. Bowman, Kyle B. Spainhower, Ryan M. Murphy, Erik S. Knudsen, Suzanne E. Fenton, Scott H. Randell, Jeremiah R. Holt, D. Neil Hayes, Agnieszka K. Witkiewicz, Trudy G. Oliver, M. Ben Major, Bernard E. Weissman
Summary: This study demonstrates the prevalence of Nrf2 (E79Q/+) mutation in human tumors, and suggests a role for NRF2 activation in the development of small cell lung cancer (SCLC).
Article
Biochemistry & Molecular Biology
David C. Lamb, Tatiana Y. Hargrove, Bin Zhao, Zdzislaw Wawrzak, Jared Goldstone, William David Nes, Steven L. Kelly, Michael R. Waterman, John J. Stegeman, Galina Lepesheva
Summary: Sterol biosynthesis in Methylococcus capsulatus involves a simplified pathway with the key step of sterol 14 alpha-demethylation catalyzed by CYP51. The structure of CYP51fx from M. capsulatus is the first of a bacterial sterol biosynthetic enzyme, suggesting a common evolutionary origin of eukaryotic and bacterial CYP51s. Bioinformatic analysis reveals the presence of CYP51 genes in over 1,000 bacteria, supporting the idea that early eukaryotes may have recruited CYP51 from bacterial sources.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Editorial Material
Chemistry, Medicinal
Mele N. Avilla, Christopher A. Bradfield, Ed Glover, Mark E. Hahn, Kristen M. C. Malecki, Paula H. Stern, Rachel H. Wilson
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Environmental Sciences
Malin C. Celander, Jared Goldstone, Nadja R. Brun, Bryan Clark, Saro Jayaraman, Diane Nacci, John J. Stegeman
Summary: The study demonstrated differential effects of PCB153 and PCB126 on Cyp3a56 mRNA expression in gills and liver of Atlantic killifish from contaminated and reference sites, indicating a potential heritable resistance to non-dioxin-like PCBs. Induction of Cyp3a56 in the gills may serve as a biomarker for exposure to non-dioxin-like PCBs in fish populations.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2021)
Editorial Material
Engineering, Environmental
Bryan D. James, Mark E. Hahn, Christopher M. Reddy
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Cory A. Berger, Collin P. Ward, Sibel I. Karchner, Robert K. Nelson, Christopher M. Reddy, Mark E. Hahn, Ann M. Tarrant
Summary: The study found that weathered oil and co-exposure to UV radiation greatly enhance the stress response of the sea anemone. Bioinformatic analysis suggests phototoxicity and oxidative damage as mechanisms for this enhanced response, which shares similarities with the oxidative stress response in vertebrates.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Nadja R. Brun, Matthew C. Salanga, Francisco X. Mora-Zamorano, David C. Lamb, Jared Goldstone, John J. Stegeman
Summary: Orphan cytochrome P450 enzyme CYP20A1 has been found to play an important role in brain function and early embryonic development in human and zebrafish. Zebrafish cyp20a1 mutants created using CRISPR/Cas9 technology exhibited significant behavioral differences from wild-type fish, showing characteristics such as anxiety and hyperactivity. This suggests that orphan CYP20A1 may have a neurophysiological role.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Nadja R. Brun, Jennifer M. Panlilio, Kun Zhang, Yanbin Zhao, Evgeny Ivashkin, John J. Stegeman, Jared Goldstone
Summary: The study demonstrates that early exposure to polychlorinated biphenyls affects neural development and behavior in zebrafish, leading to sensory deficits and disruption of dopaminergic and GABAergic signaling. PCB153-induced swelling of afferent sensory neurons and disruption of neurotransmitter signaling contribute to motor deficits in zebrafish. The findings suggest that non-dioxin-like PCBs may contribute to neurodevelopmental abnormalities in humans and exert selection pressures on vertebrate wildlife.
COMMUNICATIONS BIOLOGY
(2021)
Review
Engineering, Environmental
Wanglong Zhang, Heidi Qunhui Xie, Yunping Li, Mingxi Zhou, Zhiguang Zhou, Renjun Wang, Mark E. Hahn, Bin Zhao
Summary: The aryl hydrocarbon receptor (AHR) is a transcription factor with diverse biological functions. While initial research focused on its role in regulating drug-metabolizing enzymes and mediating the toxicity of dioxins and related compounds, recent studies have discovered additional functions of AHR. The structural similarity of emerging DLCs to dioxins has led to the observation that AHR predominantly mediates the toxic effects of these compounds. Understanding the mechanisms and impacts of traditional and emerging DLCs is important for environmental science.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biology
Djamal Brahim Belhaouari, Gabriel Augusto Pires De Souza, David C. Lamb, Steven L. Kelly, Jared Goldstone, John J. Stegeman, Philippe Colson, Bernard La Scola, Sarah Aherfi
Summary: The discovery of giant viruses challenges the definition of viruses as non-living organisms due to their exceptional genome properties and encoding of some protein synthesis machinery. Recent studies have found metabolic genes involved in energy production in giant virus genomes, blurring the boundaries between viruses and living organisms.
Article
Multidisciplinary Sciences
Liliana M. Sanmarco, Chun-Cheih Chao, Yu-Chao Wang, Jessica E. Kenison, Zhaorong Li, Joseph M. Rone, Claudia M. Rejano-Gordillo, Carolina M. Polonio, Cristina Gutierrez-Vazquez, Gavin Piester, Agustin Plasencia, Lucinda Li, Federico Giovannoni, Hong-Gyun Lee, Camilo Faust Akl, Michael A. Wheeler, Ivan Mascanfroni, Merja Jaronen, Moneera Alsuwailm, Patrick Hewson, Ada Yeste, Brian M. Andersen, Diana G. Franks, Chien-Jung Huang, Millicent Ekwudo, Emily C. Tjon, Veit Rothhammer, Maisa Takenaka, Kalil Alves de Lima, Mathias Linnerbauer, Lydia Guo, Ruxandra Covacu, Hugo Queva, Pedro Henrique Fonseca-Castro, Maha Al Bladi, Laura M. Cox, Kevin J. Hodgetts, Mark E. Hahn, Alexander Mildner, Joshua Korzenik, Russ Hauser, Scott B. Snapper, Francisco J. Quintana
Summary: Genome-wide association studies have identified risk loci linked to inflammatory bowel disease (IBD). However, the role of environmental factors in IBD susceptibility and severity has been understudied. In this study, an integrated systems approach was used to identify environmental factors and mechanisms that control intestinal inflammation.
Article
Biochemistry & Molecular Biology
Emily S. Marques, Emily G. Severance, Bellis Min, Paige Arsenault, Sarah M. Conlin, Alicia R. Timme-Laragy
Summary: Dimethyl fumarate (DMF) is used to treat inflammatory diseases, but it can have adverse effects on offspring during pregnancy. This study investigated the effects of DMF-induced Nrf2 activation during different stages of embryonic zebrafish development. The results showed that Nrf2a mutant fish had increased survival but more severe deformities during the hatching stage. Furthermore, DMF affected Nrf2a protein levels and S-glutathionylation in a tissue-specific manner. This study highlights the importance of critical windows of exposure and Nrf2a activity in the toxicity of DMF and provides insights into tissue-specific changes during embryonic development.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Engineering, Environmental
Bryan D. James, Sibel I. Karchner, Anna N. Walsh, Neelakanteswar Aluru, Diana G. Franks, Kallen R. Sullivan, Christopher M. Reddy, Collin P. Ward, Mark E. Hahn
Summary: Sunlight can transform plastic into water-soluble products, which may have potential toxicity for vertebrate animals. In this study, we exposed zebrafish larvae to photoproduced and dark leachates from different types of polyethylene bags and films, and evaluated their acute toxicity and gene expression. While we observed no acute toxicity, RNA sequencing revealed differences in the number of differentially expressed genes for each leachate treatment. Gene ontology analysis suggested that the additive-free polyethylene leachates disrupted neuromuscular processes, particularly for the photoproduced leachates. We also found that the composition of photoproduced leachates from conventional bags differed due to titanium dioxide-catalyzed reactions, which may explain the fewer differentially expressed genes elicited by these leachates. This study highlights the product formulation-specific potential toxicity of photoproduced plastic products on gene expression in zebrafish larvae.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Emily R. Leonard, Emily S. Marques, Monika A. Roy, Sarah M. Conlin, Ravi Ranjan, Alicia R. Timme-Laragy
Summary: This study investigates the impact of dietary tBHQ consumption on survival, growth parameters, organ development, and gene expression in zebrafish. The findings demonstrate that dietary tBHQ exposure impairs growth and survival and upregulates immune system-related pathways.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Engineering, Environmental
Bryan D. James, Christopher M. Reddy, Mark E. Hahn, Robert K. Nelson, Asha de Vos, Lihini I. Aluwihare, Terry L. Wade, Anthony H. Knap, Gopal Bera
Summary: In May 2021, the M/V X-Press Pearl containership burned for 2 weeks, causing the largest maritime spill of resinpellets. The spill consisted of nurdles exposed to heat and combustion, burnt plastic pieces, and oil-plastic agglomerates. The burned plastic had a high content of polycyclic aromatic hydrocarbons (PAHs), suggesting the need to classify it as hazardous waste. The disaster highlights the need to re-evaluate the handling and disposal of plastic debris collected from beaches.
ACS ENVIRONMENTAL AU
(2023)
Article
Engineering, Environmental
Bryan D. James, Asha de Vos, Lihini I. Aluwihare, Sarah Youngs, Collin P. Ward, Robert K. Nelson, Anna P. M. Michel, Mark E. Hahn, Christopher M. Reddy
Summary: In late May 2021, a container ship caught fire near the coast of Colombo, Sri Lanka, resulting in a massive release of plastic nurdles. These nurdles underwent various changes due to combustion and heat exposure, including discoloration and partial combustion. The study's findings emphasize the need to explore the impact of partially combusted plastic on the environment and highlight the importance of timely information for managing and recovering from such spills.
ACS ENVIRONMENTAL AU
(2022)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
