Article
Virology
Zhixiang Xu, Yun Peng, Minghui Yang, Xiaohe Li, Jun Wang, Rongrong Zou, Jinhu Liang, Shisong Fang, Yingxia Liu, Yang Yang
Summary: Due to the prevalence and risk of coinfection of multiple arboviruses, a multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection, with high specificity and sensitivity.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Public, Environmental & Occupational Health
Cristiane Cunha Frota, Francisco Gustavo Silveira Correia, Lucas Romao Alves Vasconcelos, Paulo Rafael Cardoso de Sousa, Marco Levi da Silva Ferreira, Susy Pereira Saraiva, Rafael Mota Ferreira, Kalil Andrade Mubarac Romcy, Ronald Feitosa Pinheiro, Roberta Taiane Germano de Oliveira, Francisco Marto Leal Pinheiro Junior, Adriano Ferreira Martins, Carlos Sanhueza-Sanzana, Rosa Livia Freitas de Almeida, Carl Kendall, Ivo Castelo Branco Coelho, Roberto da Justa Pires Neto, Italo Wesley Oliveira Aguiar, Ligia Regina Franco Sansigolo Kerr
Summary: In Fortaleza, Brazil, there was simultaneous circulation and co-infection of Zika virus (ZIKV), dengue virus (DENV), and chikungunya virus (CHIKV) in women after a Zika epidemic. Socioeconomic and environmental factors associated with arbovirus positivity included low socioeconomic class and the presence of a water box sealing in the household.
PATHOGENS AND GLOBAL HEALTH
(2023)
Article
Microbiology
Marcus Vinicius de Franca Cirilo, Shahab Zaki Pour, Viviane de Fatima Benedetti, Jessica Pires Farias, Mayanna Moreira Costa Fogaca, Rafael da Conceicao Simoes, Paloma Oliveira Vidal, Alexander Birbrair, Paolo Marinho de Andrade Zanotto, Wilson Barros Luiz, Jaime Henrique Amorim
Summary: This study conducted research in two main cities of Western Bahia, Brazil, and used molecular biology assays to detect various mosquito-borne viruses, including CHIKV, DENV, and ZIKV. The results showed the co-circulation of these viruses in the region, highlighting the importance of laboratory diagnosis in monitoring disease transmission.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Pediatrics
Celia D. C. Christie, Aileen M. Lue, Roxanne H. Melbourne-Chambers
Summary: This review discusses the impact of dengue, chikungunya, and zika on children in the Caribbean. Dengue is becoming more severe in the region and has increased morbidity and mortality in children. Chikungunya had an explosive epidemic in the Caribbean, with children under 5 years old being the most affected. Zika has a seroprevalence of 15% in pregnancy and causes various complications in children.
CURRENT OPINION IN PEDIATRICS
(2023)
Article
Multidisciplinary Sciences
Eny Regina da Silva Queiroz, Roberto de Andrade Medronho
Summary: There was no agreement between the hotspots of the dengue fever historical series (2000-2014) and those of the emerging Zika fever and chikungunya fever (2015-2019). However, there was agreement between hotspots of the three arboviral diseases between 2015 and 2019.
Article
Infectious Diseases
Gaspary O. Mwanyika, Calvin Sindato, Sima Rugarabamu, Susan F. Rumisha, Esron D. Karimuribo, Gerald Misinzo, Mark M. Rweyemamu, Muzamil M. Abdel Hamid, Najmul Haider, Francesco Vairo, Richard Kock, Leonard E. G. Mboera
Summary: The study in Tanzania revealed circulating DENV, CHIKV, and ZIKV across diverse ecological zones. Factors like increasing age, frequent mosquito bites, and presence of stagnant water were associated with higher seropositivity rates. Strengthening mosquito-borne viral disease control in Tanzania is warranted.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Pathology
Antonio Mori, Elena Pomari, Michela Deiana, Francesca Perandin, Sara Caldrer, Fabio Formenti, Manuela Mistretta, Pierantonio Orza, Andrea Ragusa, Chiara Piubelli
Summary: Molecular technology is important in arboviruses diagnostics, with PCR-based methods standing out in terms of sensitivity and cost. While commercial diagnostic molecular assays are available, they are often not affordable for resource-limited settings and not suitable for point-of-care/point-of-need use. Improvement in current molecular assays is needed to optimize their translation to the point of care.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Review
Virology
Maheshi Mapalagamage, Daniela Weiskopf, Alessandro Sette, Aruna Dharshan De Silva
Summary: Arboviral infections like CHIKV, DENV, and ZIKV are significant disease burdens in tropical and sub-tropical countries without effective vaccines or treatments available. The role of T cell response is crucial in designing effective vaccines, with CD8 and CD4 T cells playing important roles in the protective and pathogenic aspects of DENV infections.
Article
Infectious Diseases
Jaime E. Castellanos, Nayeli Jaimes, Carolina Coronel-Ruiza, Juan Pablo Rojas, Luis F. Mejia, Victor H. Villarreal, Luz E. Maya, Luz M. Claros, Cielo Orjuela, Eliana Calvo, Maria Victoria Munoz, Myriam L. Velandia-Romero
Summary: A study conducted in a pediatric clinic in Cali, Colombia, identified CHIKV as the arbovirus causing febrile cases in children, potentially leading to double infections. The results emphasize the importance of clinical tests to determine the arbovirus involved in infectious diseases for better treatment and surveillance.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Femke W. Overbosch, Janke Schinkel, Amy Matser, Gerrit Koen, Irene Prange, Maria Prins, Gerard J. B. Sonder
Summary: Dutch travellers to Suriname, especially tourists, are at substantial risk of DENV infection. The introduction of ZIKV and CHIKV in Suriname has also increased the risk of infection. The potential cross-reactivity and cross-protection of anti-DENV and -ZIKV antibodies should be further explored.
Article
Infectious Diseases
Pablo Cantalice Santos Farias, Andre Filipe Pastor, Juliana Prado Goncales, Iasmyn Dayanne Santos do Nascimento, Ellida Suellen de Souza Ferraz, Thaisa Regina Rocha Lopes, Rodrigo Feliciano do Carmo, Maria Rosangela Cunha Duarte Coelho, Jose Valter Joaquim Silva Junior
Summary: This study was conducted in Recife, Pernambuco state, Brazil to investigate the influence of chikungunya (CHIKV) and Zika (ZIKV) viruses on the epidemiological profile of dengue cases. The results showed that although females were the main population diagnosed with dengue, chikungunya, and Zika infections, there were important differences in the age distribution of these viruses, which should be considered in public health policies and future studies of virus-host interaction.
BMC INFECTIOUS DISEASES
(2023)
Review
Microbiology
Awadalkareem Adam, Christian Jassoy
Summary: Arbovirus infections are prevalent in Africa due to the lack of established epidemiological surveillance systems and accurate differential diagnosis. Studies indicate these infections are endemic in most African countries, highlighting the need for improved monitoring to enhance diagnostic accuracy. Healthcare providers should be informed about prevalent arboviral diseases to better identify and manage possible cases.
Article
Parasitology
Silvia Tortosa-La Osa, Eva Martin-Ruiz, Angela Galan-Relano, Antonio Olry de Labry-Lima
Summary: This systematic review evaluates the effectiveness of environmental interventions for the prevention and reduction of vector-borne diseases such as dengue, Zika, and chikungunya. The findings suggest that interventions such as eliminating small containers, manipulating large tanks, and cleaning outdoor spaces can significantly reduce pupae indices, with easy implementation and low resource requirements. However, the evidence on whether reducing mosquito abundance leads to a reduction in disease transmission risk is limited. It is recommended to consider epidemiological, environmental, and sociodemographic factors in addition to entomological indicators.
Article
Automation & Control Systems
Andrew Omame, Mary Ele Isah, Mujahid Abbas
Summary: The co-circulation of different emerging viral diseases poses a significant challenge in epidemiology. This article develops a mathematical model to assess the impact of COVID-19 on the dynamics of zika, chikungunya, and dengue, as well as vice-versa. The study shows that COVID-19 prevention measures can greatly reduce the burden of co-infections with other diseases, while the prevention measures for zika, dengue, and chikungunya can also significantly reduce the burden of co-infections with COVID-19.
OPTIMAL CONTROL APPLICATIONS & METHODS
(2023)
Article
Infectious Diseases
Maria Fernanda Avila Mejia, Pei-Yun Shu, Dar-Der Ji
Summary: Dengue, Chikungunya, and Zika are co-endemic in Honduras and are often misdiagnosed due to similar clinical and epidemiological behavior. Only 30.84% of diagnostic accuracy was observed in physicians in primary care when comparing arboviral clinical diagnosis with RT-qPCR detection. Unspecific symptomatology, overlapping case definitions, and reported close contact to an arboviral patient might contribute to misdiagnosis.
BMC INFECTIOUS DISEASES
(2023)
Letter
Infectious Diseases
Chloe Dimeglio, Fabrice Herin, Guillaume Martin-Blondel, Marcel Miedouge, Jacques Izopet
JOURNAL OF INFECTION
(2022)
Article
Infectious Diseases
Florence Ader, Maude Bouscambert-Duchamp, Maya Hites, Nathan Peiffer-Smadja, Julien Poissy, Drifa Belhadi, Alpha Diallo, Minh-Patrick Le, Gilles Peytavin, Therese Staub, Richard Greil, Jeremie Guedj, Jose-Artur Paiva, Dominique Costagliola, Yazdan Yazdanpanah, Charles Burdet, France Mentre
Summary: A study conducted in COVID-19 patients admitted to hospital and requiring oxygen support found that remdesivir did not show significant clinical benefits compared to standard care alone.
LANCET INFECTIOUS DISEASES
(2022)
Article
Immunology
Paul Dalla-Pozza, Maxime Hentzien, Clotilde Allavena, Anne Doe de Maindreville, Kevin Bouiller, Marc-Antoine Valantin, Emmanuel Lafont, Olivia Zaegel-Faucher, Antoine Cheret, Guillaume Martin-Blondel, Laurent Cotte, Firouze Bani-Sadr
Summary: This study highlights that factors other than low CD4(+) cell count and high HIV viral load may be associated with the occurrence of PML in people with HIV. Further studies are warranted to investigate in greater detail the immunologic characteristics of people with HIV who develop PML despite immune-virological control.
Letter
Hematology
Guillaume Moulis, Johanne Germain, Manuela Rueter, Margaux Lafaurie, Myriam Aroichane, Thibault Comont, Matthieu Mahevas, Jean-Francois Viallard, Stephane Cheze, Mikael Ebbo, Sylvain Audia, Soraya Leclerc-Teffahi, Agnes Sommet, Odile Beyne-Rauzy, Marc Michel, Bertrand Godeau, Maryse Lapeyre-Mestre
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Letter
Infectious Diseases
Guillaume Martin-Blondel, Anne-Genevieve Marcelin, Cathia Soulie, Sofia Kaisaridi, Clovis Lusivika-Nzinga, Celine Dorival, Laura Nailler, Anais Boston, Clea Melenotte, Geraldine Gaube, Christophe Choquet, Roland Liblau, Fabrice Carrat, Youri Yordanov
JOURNAL OF INFECTION
(2022)
Letter
Public, Environmental & Occupational Health
Laurie Windels, Veronique Naneix-Laroche, Christophe Pasquier, Pierre Delobel, Perrine Parize, Guillaume Martin-Blondel
JOURNAL OF TRAVEL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Remi Planes, Miriam Pinilla, Karin Santoni, Audrey Hessel, Charlotte Passemar, Kenneth Lay, Perrine Paillette, Ana-Luiza Chaves Valadao, Kim Samirah Robinson, Paul Bastard, Nathaniel Lam, Ricardo Fadrique, Ida Rossi, David Pericat, Salimata Bagayoko, Stephen Adonai Leon-Icaza, Yoann Rombouts, Eric Perouzel, Michele Tiraby, Qian Zhang, Pietro Cicuta, Emmanuelle Jouanguy, Olivier Neyrolles, Clare E. Bryant, Andres R. Floto, Caroline Goujon, Franklin Zhong Lei, Guillaume Martin-Blondel, Stein Silva, Jean-Laurent Casanova, Celine Cougoule, Bruno Reversade, Julien Marcoux, Emmanuel Ravet, Etienne Meunier
Summary: The activation of inflammasomes plays a crucial role in regulating the infection of SARS-CoV-2. Lung epithelial cells serve as the primary entry site of the virus and express the inflammasome-forming sensor NLRP1. The cleavage of NLRP1 by coronavirus 3CL proteases leads to inflammasome assembly, cell death, and reduced production of viral particles. Additionally, these proteases also inactivate the executioner protein Gasdermin D, promoting an alternative form of cell death called pyroptosis. Analysis of pyroptosis markers in COVID-19 patients suggests that GSDME/caspase-3 could potentially serve as markers of disease severity.
Article
Immunology
Alexandra Serris, Amani Ouedrani, Fabrice Uhel, Marianne Gazzano, Vincent Bedarida, Claire Rouzaud, Marie-Elisabeth Bougnoux, Jean-Herle Raphalen, Sylvain Poiree, Olivier Lambotte, Guillaume Martin-Blondel, Fanny Lanternier
Summary: Invasive fungal diseases (IFD) continue to be a major cause of morbidity and mortality, necessitating the development of new treatment approaches. Recent data suggest that checkpoint inhibitors (ICI) may have a beneficial effect. This report presents a case of a diabetic patient with refractory IFD following SARSCoV-2 infection, who was treated with ICI and interferon-gamma in combination with antifungal therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Marion Migueres, Chloe Dimeglio, Jean Michel Mansuy, Florence Abravanel, Stephanie Raymond, Justine Latour, Nicolas Jeanne, Noemie Ranger, Sebastien Lhomme, Karine Saune, Pauline Tremeaux, Jacques Izopet
Summary: Using variant typing polymerase chain reaction, this study describes the evolution of SARS-CoV-2 Omicron sublineages between December 2021 and mid-March 2022. The advantage of the BA.2 variant over BA.1 is not attributed to greater nasopharyngeal viral loads.
CLINICAL INFECTIOUS DISEASES
(2023)
Review
Infectious Diseases
Florence Abravanel, Sebastien Lhomme, Olivier Marion, Jean Marie Peron, Nassim Kamar, Jacques Izopet
Summary: HEV was initially thought to cause only acute infections, but the discovery of chronic hepatitis E in immunocompromised patients has changed our understanding of the virus. This article describes the physiopathology, diagnosis, and clinical management of chronic HEV infection. The available treatment options are limited to ribavirin and interferon, and new drugs are urgently needed.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Biochemistry & Molecular Biology
David Pericat, Stephen Adonai Leon-Icaza, Marina Sanchez Rico, Christiane Muehle, Iulia Zoicas, Fabian Schumacher, Remi Planes, Raoul Mazars, Germain Gros, Alexander Carpinteiro, Katrin Anne Becker, Jacques Izopet, Nathalie Strub-Wourgaft, Peter Sjoe, Olivier Neyrolles, Burkhard Kleuser, Frederic Limosin, Erich Gulbins, Johannes Kornhuber, Etienne Meunier, Nicolas Hoertel, Celine Cougoule
Summary: This study evaluated the potential antiviral and anti-inflammatory activities of fluoxetine in a SARS-CoV-2 infection model and in vitro. The results showed that fluoxetine significantly reduced lung tissue viral titers and expression of inflammatory markers, and inhibited the replication of all variants of concern. These effects may be attributed to the modulation of the ceramide system in the lung tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Sebastien Lhomme, Sebastien Magne, Sylvie Perelle, Emmanuelle Vaissiere, Florence Abravanel, Laetitia Trelon, Catherine Hennechart-Collette, Audrey Fraisse, Sandra Martin-Latil, Jacques Izopet, Julie Figoni, Guillaume Spaccaferri
Summary: The identification of seven cases of hepatitis E virus infection in a French rural hamlet in April 2015 led to investigations confirming the clustering and identifying the source of the infection. Laboratories and general practitioners in the area actively searched for other cases based on RT-PCR and serological tests. Phylogenetic analyses were performed to compare HEV sequences. No other cases were found.
During the investigation, it was discovered that all the patients drank water from the public network, but a break in the water supply to the hamlet occurred at the time the infection probably occurred. HEV RNA was also detected in a private water source that was connected to the public water network. The turbid water flowing from the taps during the break suggested contamination from the private water supply.
Article
Immunology
Chloe Dimeglio, Isabelle Da-Silva, Marion Porcheron, Marie-Pierre Panero, Laetitia Staes, Pauline Tremeaux, Helene Villars, Jacques Izopet
Summary: The effectiveness of current vaccines is lower in older people due to aging of their immune systems. A study measured the antibody responses of 42 adults in nursing homes after receiving the third and fourth doses of an mRNA vaccine. The results showed that the strain of the virus influenced the effect of the fourth dose on neutralizing antibodies. The fourth dose increased binding antibodies, but the effect was less significant compared to the third dose. However, the fourth dose provided approximately 80% protection against a SARS-CoV-2 BA.2 infection in most individuals.
Article
Ophthalmology
Emmanuelle Loubsens, Raphael Adam, Alexa Debard, Lisa Barioulet, Fanny Varenne, Pierre Fournie, Thomas Sales de Gauzy, Priscille Olle, Guillaume Martin-Blondel, Vincent Soler
Summary: This study reviewed the management, treatment, and outcomes of patients with necrotizing herpetic retinitis (NHR) and proposed an algorithm for first-line management of NHR. The visual prognosis of NHR patients was poor and prompt investigation of immune status and presence of factors justifying intravitreal antiviral injections is necessary.
INTERNATIONAL OPHTHALMOLOGY
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
