Article
Medicine, General & Internal
Kang Chang, Haibo Wang, Jianping Zhao, Xianghong Yang, Bo Wu, Wenkui Sun, Man Huang, Zhenshun Cheng, Hong Chen, Yuanlin Song, Ping Chen, Xiangqi Chen, Xin Gan, Wanli Ma, Lihua Xing, Yimin Wang, Xiaoying Gu, Xiaohui Zou, Bin Cao
Summary: This study suggests that the appropriate polymyxin B/tigecycline combination therapy is not superior to appropriate polymyxin B therapy in terms of 28-day mortality in the treatment of hospital-acquired pneumonia caused by carbapenem-resistant organisms.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Jiayuan Zhang, Shuo Diao, Yanfei Liu, Hongxiang Wang, Yuwei Liu, Shixing Zhu, Kun Feng, Xiaoqian Tang, Charles Oo, Peijuan Zhu, Zhihua Lv, Mingming Yu, Sherwin K. B. Sy, Yuanqi Zhu
Summary: This study evaluates the potential of combining sulbactam, meropenem, and polymyxin-B to reduce the mutant selection window and resistance development against Acinetobacter baumannii harboring OXA-23. The results show that combination therapy optimizes drug concentrations and reduces the opportunity for bacteria to develop further resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Xue Zhang, Fen Qu, Wei Jia, Bin Huang, Bin Shan, Hua Yu, Yiwei Tang, Liang Chen, Hong Du
Summary: This study found that despite the lack of exposure to polymyxins, 3.8% of CRE were resistant to polymyxins in China. Both chromosomal and plasmid-encoded mechanisms of resistance were identified in resistant isolates.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Maya Beganovic, Kathryn E. Daffinee, Megan K. Luther, Kerry L. LaPlante
Summary: Triple therapy with high-dose minocycline, continuous-infusion sulbactam, and polymyxin B demonstrated the most significant kill against carbapenem-resistant Acinetobacter baumannii, with no regrowth and minimal resistance development in an in vitro pharmacodynamic model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Cheng Chen, Ke-Wu Yang, Le Zhai, Huan-Huan Ding, Jia-Zhu Chigan
Summary: The study constructed a compound library of dithiocarbamates and evaluated their synergistic antibacterial activities combined with copper. One compound was found to inhibit the growth and resistance development of NDM-1-producing Gram-negative pathogens, and a novel inhibition mechanism was uncovered.
BIOORGANIC CHEMISTRY
(2022)
Article
Microbiology
Dongna Zou, Guangyue Yao, Chengwu Shen, Jinru Ji, Chaoqun Ying, Peipei Wang, Zhiying Liu, Jun Wang, Yan Jin, Yonghong Xiao
Summary: This study aimed to predict and evaluate three antimicrobials for treatment of adult bloodstream infections with CRE in China, and suggested that individualized therapy and measurement of MICs should be considered together to achieve optimal drug exposure. Pharmacokinetic and pharmacodynamic modeling based on local antimicrobial resistance data can provide valuable guidance for clinicians for the administration of empirical antibiotic treatments for BSIs.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Hasini Wickremasinghe, Heidi H. Yu, Mohammad A. K. Azad, Jinxin Zhao, Phillip J. Bergen, Tony Velkov, Qi Tony Zhou, Yan Zhu, Jian Li
Summary: The combination of polymyxin B and meropenem demonstrated synergistic bacterial killing against biofilm-producing, polymyxin-resistant MDR Pseudomonas aeruginosa, reinstating the efficacy of two previously ineffective antibiotics and providing a potential treatment strategy for difficult-to-treat infections caused by these pathogens.
Article
Chemistry, Medicinal
Chungsik Kim, Mintesinot Kassu, Kenneth P. Smith, James E. Kirby, Roman Manetsch
Summary: A total of 20 novel derivatives of compound KP-40 containing Pyrazole-thiazole core were designed and synthesized through traditional SAR analysis. Four of the compounds showed significant adjunctive potential with improved solubility, and key physicochemical properties for the development of active Gram-negative antibacterials were revealed through further analysis using PEMPO algorithm. Consideration of PEMPO scores prior to synthesis of analogs may be a simple yet effective strategy in conjunction with traditional SAR approaches for designing potent Gram-negative antibacterials.
Article
Microbiology
Shixing Zhu, Chu Song, Jiayuan Zhang, Shuo Diao, Marc Tobias M. Heinrichs, Frederico S. Martins, Zhihua Lv, Yuanqi Zhu, Mingming Yu, Sherwin K. B. Sy
Summary: The combination of amikacin, polymyxin-B, and sulbactam can effectively treat multidrug-resistant Acinetobacter baumannii and reduce the risk of further resistance. However, their efficacy in the lung is not optimal.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Shixing Zhu, Jiayuan Zhang, Chu Song, Yuwei Liu, Charles Oo, M. Tobias Heinrichs, Zhihua Lv, Yuanqi Zhu, Sherwin K. B. Sy, Pan Deng, Mingming Yu
Summary: This study investigated the synergistic effects of polymyxin-B, meropenem, and sulbactam combination therapy on multi-drug resistant Acinetobacter baumannii using metabolomic analysis. The results showed that the triple-antibiotic combination significantly changed the levels of metabolites involved in cell outer membrane and cell wall biosynthesis. Moreover, the combination therapy disrupted nucleotide and amino acid biosynthesis pathways as well as the central carbon metabolism. The addition of sulbactam appeared to enhance the antibacterial effects of polymyxin-B and meropenem by disrupting the bacterium's metabolome.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Ippei Yoshida, Iichiro Takata, Kiyoko Fujita, Hajime Takashima, Hiroyuki Sugiyama
Summary: CRE is a urgent public health threat with limited therapeutic options. TP0586532 has been found to enhance the antibacterial activity of combination antibiotics against CRE by increasing bacterial membrane permeability.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
S. Araujo, M. Sousa, M. Tacao, R. A. Barauna, A. Silva, R. Ramos, A. Alves, C. M. Manaia, I. Henriques
Summary: Although urban wastewater treatment plants play a crucial role in eliminating antibiotic resistance, they still struggle to produce effluents free of resistance. UV-C radiation significantly reduces the abundance and prevalence of carbapenem-resistant bacteria (CRB), but CRB numbers increased after incubation in the dark. Carbapenem-resistant Enterobacteriaceae (CRE) with bla(GES-5), integrons, and plasmids were detected in samples, raising concerns about potential horizontal gene transfer within these systems. Surveillance of carbapenem resistance in UWWTPs is essential for timely mitigation measures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
O. H. F. Raro, G. S. Collar, R. M. C. daSilva, P. Vezzaro, M. P. Mott, G. R. daCunha, C. V. W. Riche, C. Dias, J. Caierao
Summary: This study evaluated the accuracy of agar-based methods for susceptibility testing in CRE, with good performance observed. The agar screening test using polymyxin B instead of colistin is proposed for the first time.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Feiyang Zhang, Qin Li, Jiawei Bai, Manlin Ding, Xiangjin Yan, Guangxi Wang, Baoli Zhu, Yingshun Zhou
Summary: This study revealed that 8.39% of Klebsiella pneumoniae strains were amikacin-heteroresistant, with potential association with increased expression of aminoglycoside resistance genes. The majority of heteroresistant phenotypes were unstable, with partial or full reversion of minimal inhibitory concentrations to the level of susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Zhen Liang, Yun-Long Shi, Yuan Peng, Chen Xu, Cheng Zhang, Yu Chen, Xiao-Qiang Luo, Qi-Meng Li, Cheng-Lei Zhao, Jie Lei, Zhi-Qiang Yuan, Yi-Zhi Peng, Bao-Qiang Song, Ya-Li Gong
Summary: This study isolated and evaluated a phage BL02 that is resistant to both carbapenem and polymyxin-B in CRKP, which poses a threat to public health in Southwestern China. BL02 showed high lytic activity against CRKP isolates and has potential applications in treating polymyxin-B resistant K. pneumoniae.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)