Article
Fisheries
Valentina Valenzuela-Munoz, Barbara P. Benavente, Antonio Casuso, Yeny Leal, Diego Valenzuela-Miranda, Gustavo Nunez-Acuna, Constanza Saez-Vera, Cristian Gallardo-Escarate
Summary: This study demonstrates that immunizing Atlantic salmon with IPath(R) can significantly modulate the transcriptome of sea lice and induce abnormal reproductive development and inflammatory responses. Furthermore, downregulation of multiple immune-related and iron homeostasis-related genes was observed in the head kidney and skin tissues of immunized fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Diego Valenzuela-Miranda, Valentina Valenzuela-Munoz, Barbara P. Benavente, Marcelo Munoz-Trorcoso, Gustavo Nunez-Acuna, Cristian Gallardo-Escarate
Summary: Parasitism by sea lice affects the diversity of microbial communities in Atlantic salmon, leading to the occurrence of opportunistic bacterial pathogens and perturbation of the correlation between skin and gut microbiota. Sea lice can act as vectors for transmitting specific bacterial species and induce tissue-specific functional dysbiosis.
Article
Biology
Debora Torrealba, Byron Morales-Lange, Victoriano Mulero, Anti Vasemagi, Luis Mercado, Jose Gallardo-Matus
Summary: This study estimated the heritabilities of three key proteins associated with the innate immunity and resistance of Atlantic salmon against sea lice. It found that susceptible fish had higher levels of Nkef and Tnf alpha in their gills and skin, while resistant fish had higher levels of Il-8 in their gills. The heritabilities of Nkef and Tnf alpha were moderate to high, while the heritability of Il-8 was low.
Article
Multidisciplinary Sciences
G. F. Difford, J-E Haugen, M. L. Aslam, L. H. Johansen, M. W. Breiland, B. Hillestad, M. Baranski, S. Boison, H. Moghadam, C. Jacq
Summary: The presence of certain volatile organic compounds (VOCs) in the mucus of salmon hosts after salmon lice infection is significantly associated with lice infection numbers. These compounds may have a genetic component and can serve as a more direct and ethical proxy for salmon lice resistance.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Scarleth Bravo, Francisco Leiva, Javier Moya, Osiel Guzman, Rodrigo Vidal
Summary: Sea lice are harmful marine parasites that pose a serious threat to the global salmon industry. Transcriptomic investigations have focused on gene expression response of Atlantic salmon to sea lice, but have overlooked the important role of alternative splicing (AS) in gene expression control. This study identified a significant increase in AS events and AS genes after sea lice infestation, and found a higher number of AS events and genes in resistant and susceptible phenotypes. Functional annotation of AS genes revealed their association with behavior, RNA splicing, immune response, and RNA binding.
MARINE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Aina-Cathrine Overgard, Helena M. D. Midtbo, Lars A. Hamre, Michael Dondrup, Gro E. K. Bjerga, Oivind Larsen, Jiwan Kumar Chettri, Kurt Buchmann, Frank Nilsen, Sindre Grotmol
Summary: This study reveals the presence of charged glandular proteins in the salmon louse that have immune modulation and anti-coagulant properties. These proteins play important roles in regulating the parasite's life cycle and feeding behavior in the host.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Marco Rozas-Serri, Rodolfo Correa, Romina Walker-Vergara, Darling Conuecar, Soraya Barrientos, Camila Leiva, Ricardo Ildefonso, Carolina Senn, Andrea Pena
Summary: This study provides integrated reference intervals for 44 blood biomarkers for different age groups of intensively farmed Atlantic salmon, coho salmon, and rainbow trout in Chile. The variability of blood biomarkers depends on species, age range, and their interactions, highlighting the importance of improving health and welfare management for farmed fish.
Article
Fisheries
Robert H. Devlin, Carlo A. Biagi, Dionne Sakhrani, Takafumi Fujimoto, Rosalind A. Leggatt, Jack L. Smith, Timothy Y. Yesaki
Summary: Pacific salmon and Atlantic salmon can hybridize, but only a small number of embryos survive to sexual maturity, mainly at early developmental stages. These intergeneric crosses are expected to have severe fitness consequences for both dams and sires in natural environments.
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Qing Xia, Jian-Xin Cheng, Ya-Fang Liu, Cheng-Hua Li, Ying Liu, Peng-Fei Liu
Summary: This study comprehensively analyzed the profiles of lncRNAs, miRNAs, and mRNAs in gills of Atlantic salmon under high and low-dose A. salmonicida infection as well as a control group. Key genes in immune-related pathways were predicted and a lncRNA-miRNA-mRNA network was constructed to identify potential novel biomarkers in regulating the immune response of Atlantic salmon against A. salmonicida infection.
Article
Fisheries
Paulina Bustos, Carolina Figueroa, Barbara Cadiz, Tamara Santander, Brian Dixon, Jose A. Gallardo, Pablo Conejeros
Summary: Recently, it was found that Atlantic salmon vaccinated against Piscirickettsia salmonis lose their protection when co-infected with Caligus rogercresseyi. The reasons for this effect and the molecular basis of the immune responses in vaccinated salmon during C. rogercresseyi infection have not been determined. In this study, changes in immune gene expression were investigated in vaccinated Atlantic salmon co-infected with C. rogercresseyi and P. salmonis. The results showed that the co-infection had a similar effect on immune gene expression as a single P. salmonis infection, suggesting a decreased cellular response. However, some individual fish exhibited a significantly higher immune response, indicating variation in vaccine efficacy.
JOURNAL OF FISH DISEASES
(2023)
Article
Biology
Jessica L. McKenzie, H. Andres Araujo, Jack L. Smith, Dolph Schluter, Robert H. Devlin
Summary: Global change is causing unprecedented alterations in ecosystems, potentially leading to hybridization between different species and affecting genetic diversity, adaptive capacity, and evolutionary paths. This study on Chinook and Coho salmon found potential and consequences of hybridization, including postzygotic developmental barriers. Climate change could further influence hybridization between these species, with unknown impacts on the future of these threatened salmon and their habitats.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Review
Food Science & Technology
Sherry Stephanie Chan, Bjorn Roth, Flemming Jessen, Anita Nordeng Jakobsen, Jorgen Lerfall
Summary: This review focuses on the water holding properties of Atlantic salmon, evaluating the methods used to measure them and how pre- and postmortem factors, processing, and preservation methods influence these properties and their correlations to other quality parameters. Various methods are available to measure water holding capacity (WHC), with the centrifugation method being the most prevalent but other non-invasive and cost-effective approaches increasingly preferred. The diversity of methods makes direct comparison of results from previous research relative unless the same method is used with the same conditions.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Multidisciplinary Sciences
Amy Long, Simon R. M. Jones
Summary: Salmonid rickettsial septicemia is an emergent disease caused by Piscirickettsia salmonis in marine-farmed salmonids. The study found species-specific patterns of bacterial shedding and pathogenesis, as well as correlations between physiological parameters and bacterial burden in different fish species. The timing and magnitude of bacterial shedding in infected fish align with mortality, providing insights for pathogen transmission models.
Article
Agriculture, Dairy & Animal Science
Victor Martinez, Phillip J. Dettleff, Nicolas Galarce, Cristian Bravo, Jessica Dorner, Robert N. Iwamoto, Kerry Naish
Summary: Understanding the genetic status of aquaculture strains is crucial for sustainable management. Coho salmon in Chile's aquaculture industry has faced various events that have shaped its genome. Comparisons with strains from other countries revealed differences in effective population size, highlighting the need for measures to maintain genetic diversity and long-term sustainability.
Review
Food Science & Technology
Jeremy D. Landry, Ewan W. Blanch, Peter J. Torley
Summary: This review discusses the chemical quality markers of Atlantic salmon, including lipids and pigmentation. These markers are influenced by diet and other important factors. Various analysis methods are used to monitor these quality parameters, with some considered industry standard. However, recent advances in spectroscopic techniques offer faster and non-invasive alternatives with great potential in fisheries science.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Fisheries
Jaewoon Jeong, Gregor F. McEwan, Gabriel Arriagada, Cristian Gallardo-Escarate, Crawford W. Revie
Summary: The salmon louse C. rogercresseyi is a common ectoparasite of farmed salmonids in Chile, and further research is required to adequately model its complete population dynamics and support effective control and mitigation on Chilean salmon farms.
JOURNAL OF FISH DISEASES
(2022)
Correction
Multidisciplinary Sciences
Diego Valenzuela-Miranda, Ana Teresa Goncalves, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Ivan Liachko, Bradley Nelson, Cristian Gallardo-Escarate
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Francisca Samsing, James W. Wynne, Valentina Valenzuela-Munoz, Diego Valenzuela-Miranda, Cristian Gallardo-Escarate, Pamela A. Alexandre
Summary: This study utilized transcriptome sequencing data to explore gene regulatory networks in Atlantic salmon infected with a virus. The results revealed a specific miRNA cluster that was strongly induced, potentially serving as a biomarker for early infection. Additionally, a downregulated selenium protein was identified, which may be associated with viral clearance and recovery to a stable state.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Microbiology
Maria F. Morales-Rivera, Diego Valenzuela-Miranda, Gustavo Nunez-Acuna, Barbara P. Benavente, Cristian Gallardo-Escarate, Valentina Valenzuela-Munoz
Summary: This study explored the diversity and abundance of the Atlantic salmon intestinal microbiota and metagenome functional prediction during seawater transfer under three treatments. The results showed an influence of salinity changes on Atlantic salmon gut microbiota richness, diversity, and taxonomic composition. The reported knowledge can be applied to surveil the microbiome in smolt fish production, improving the performance of Atlantic salmon to seawater transfer.
Article
Genetics & Heredity
Cristian Gallardo-Escarate, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Diego Valenzuela-Miranda, Fabian J. Tapia, Marco Yevenes, Gonzalo Gajardo, Jorge E. Toro, Pablo A. Oyarzun, Gloria Arriagada, Beatriz Novoa, Antonio Figueras, Steven Roberts, Marco Gerdol
Summary: The blue mussel Mytilus chilensis is an endemic and key socioeconomic species on the southern coast of Chile, supporting a booming aquaculture industry. However, its production is threatened by microorganisms, pollution, and environmental stressors. Understanding the genomic basis of local adaption is important for sustainable shellfish aquaculture.
Article
Genetics & Heredity
Valentina Valenzuela-Munoz, Cristian Gallardo-Escarate, Diego Valenzuela-Miranda, Gustavo Nunez-Acuna, Barbara P. Benavente, Alejandro Alert, Marta Arevalo
Summary: This study explored the whole-transcriptome changes in Atlantic salmon families with contrasting resistance phenotypes against lice infestation. Significant differences in gene expression profiles and SNP variations were observed between resistant and susceptible families. The findings suggest that tissue repair genes and mucosal immune activation may play a key role in Atlantic salmon resistance to sea louse infestation.
Article
Genetics & Heredity
Yeny Leal, Valentina Valenzuela-Munoz, Antonio Casuso, Barbara P. Benavente, Cristian Gallardo-Escarate
Summary: Studies have shown that SHK-1 cells and Atlantic salmon head kidney tissue were analyzed for the sea louse antigen cathepsin and found to have transcriptomic differences. Additionally, regulation through long non-coding RNAs revealed tissue-specific transcription patterns, and pathways related to the immune system and signal transduction were shared between the two tissues. These findings highlight a novel approach to evaluating candidate antigens for sea lice vaccine development, improving antigen screening in the SHK-1 cell line model.
Article
Environmental Sciences
Gustavo Nunez-Acuna, Constanza Saez-Vera, Diego Valenzuela-Miranda, Valentina Valenzuela-Munoz, Cristian Gallardo-Escarate
Summary: This study used genome resequencing and transcriptome analysis to investigate structural genomic variations and gene expression in sea lice strains with different sensitivity to pesticides. It was found that duplications or copy number variants were present in transposable elements and genes related to drug response, with a higher frequency of duplications in genes associated with cuticle proteins. The study suggests that the intensive use of pesticides increases the frequency of gene duplications and expands the molecular elements involved in drug response.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Alfredo Molina, Phillip Dettleff, Valentina Valenzuela-Munoz, Cristian Gallardo-Escarate, Juan Antonio Valdes
Summary: This study examined the effects of high-temperature stress on the skeletal muscle transcriptome of rainbow trout. The results showed increased levels of cortisol and glucose in response to high-temperature stress, but no changes in skeletal muscle oxidation were observed. Transcriptomic analysis revealed upregulation of genes related to autophagosome assembly, amino acid transport, and glutamine metabolism, as well as downregulation of genes related to digestion, proteolysis, and muscle contraction.
Article
Fisheries
Yeny Leal, Valentina Valenzuela-Munoz, Cristian Gallardo-Escarate
Summary: This study evaluated the alternative splicing transcriptome profiles during P. salmonis infection in two study models: SHK-1 cell line and salmon head kidney tissue. The highest number of differentially alternative splicing events was observed at 7 days post-infection in both models. Furthermore, alternative first exon was found to be the most abundant alternative splicing type. Functional annotation revealed a high presence of nucleotide metabolism-related genes in P. salmonis-infected SHK-1 cells, while the salmon head kidney exhibited many genes associated with immune response.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
