Article
Agricultural Engineering
Zeynab Yousefian, Yousef Hamidoghli, Pooran Golkar, Mohammad Hossein Mirjalili
Summary: Pelargonium sidoides DC. (Geraniaceae), a perennial herb from South Africa, has medicinal coumarins in its roots with confirmed anti-viral properties. In this study, transgenic hairy root lines (HRLs) of P. sidoides were created using Agrobacterium tumefaciens. Hairy root cultures (HRCs) were treated with subculture cycles and analyzed for biological and antioxidant activities. HRL4 showed the highest content of total phenols and total flavonoids. The HRC extracts had antioxidant activity and the ability to scavenge DPPH radicals and bleach beta-carotene/linoleic acid. The antioxidant activity was positively correlated with total flavonoid content.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Seungeun Baek, Jong-Eun Han, Thanh-Tam Ho, So-Young Park
Summary: This study tested the efficiency of hairy root induction in Centella asiatica using leaf and petiole explants. The results showed that petiole-derived hairy roots had the highest growth index and triterpenoid concentration. The findings suggest that hairy root culture of C. asiatica can be scaled up for mass production of triterpenoids.
Article
Plant Sciences
Minsol Choi, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, Sang Un Park
Summary: In this study, the optimal conditions for the hairy root culture of Salvia plebeia were determined, and its growth and accumulation of phenolic compounds were evaluated. The results showed that hairy roots cultured under optimal conditions exhibited enhanced phenolic compound accumulation and antioxidant activity, making them potential sources of antioxidants.
Article
Biotechnology & Applied Microbiology
Zian Zhao, Suping Gao, Ju Hu, Ting Lei, Yunzhu Zhou, Yirui Li, Cailei Liu
Summary: Cultivation of hairy roots is an effective method to obtain high plumbagin secondary metabolites. In this study, a reliable and rapid genetic transformation system for hairy root of Plumbago auriculata was established using Agrobacterium rhizogene. The results showed that ATCC 15834 had the highest hairy root transformation efficiency (86.78%) and the earliest root emergence time (8.33 days). Hairy roots induced by ATCC 15834 had a plumbagin content 72.13 times higher than untransformed roots and 3.95 times higher than 1-year-old live seedlings. This study provides an important experimental basis for the biosynthesis mechanism of plumbagin and its potential commercial production.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Alexey S. Kiryushkin, Elena L. Ilina, Elizaveta D. Guseva, Katharina Pawlowski, Kirill N. Demchenko
Summary: CRISPR/Cas-mediated genome editing and hairy root transformation are fast and effective methods for studying plant gene function. This review outlines the recent advances achieved by combining these techniques and discusses the origins of hairy root transformation strains, components of CRISPR/Cas vectors, and the application of CRISPR/Cas genome editing in hairy roots. Additionally, a method for modifying a known vector is described.
Article
Plant Sciences
Minsol Choi, Ramaraj Sathasivam, Bao Van Nguyen, Nam Il Park, Sun-Hee Woo, Sang Un Park
Summary: In this study, an efficient protocol for the culture of adventitious and hairy roots of Tartary buckwheat was developed. Different concentrations of auxins were used to induce root formation. The hairy roots showed high expression of phenolic pathway genes and accumulated a greater amount of phenolic compounds compared to other root types.
Article
Plant Sciences
Khwanlada Kobtrakul, Dolly Rani, Asma Binalee, Pattarapol Udomlarp, Tatiya Srichai, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: Cannabis sativa L. (hemp) is widely used for medicinal, food, and textile purposes. This study focused on the roots of hemp, which have received less attention than other parts. By using hairy root cultures and treating them with salicylic acid, the levels of triterpenoids friedelin and epifriedelanol in the roots were significantly increased. These findings suggest that hairy root cultures with optimized elicitation could be an alternative method to produce valuable triterpenoids.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Biotechnology & Applied Microbiology
David Humbird
Summary: The analysis reveals that due to various limitations in animal cell production, the current costs of mass producing cultured meat products are high, making it difficult to replace conventional meat. Improving metabolic efficiency and developing low-cost plant hydrolysates as media do help address the issue, but there are still challenges ahead.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Chun Fang Shen, Elodie Burney, Renald Gilbert, S. Mehdy Elahi, Kelley Parato, Martin Loignon
Summary: This study develops suspension-adapted Vero cell culture processes for high titer production of oncolytic virus HSV-1. Optimization of cell culture conditions and scaling up to bioreactors successfully achieved high titers of HSV-1. This is the first study to demonstrate high titer production of HSV-1 in suspension Vero cell culture.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Xia Luo, Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Ningqiu Li
Summary: In this study, a suspension culture system of Chinese perch brain cell (CPB) was developed for virus vaccine production, utilizing Cytodex 1 microcarrier in a stirred bioreactor.
Review
Biochemical Research Methods
Jenny Shupe, An Zhang, Daniel C. Odenwelder, Terrence Dobrowsky
Summary: Gene therapy aims to cure diseases caused by genetic defects, and rAAV is currently the preferred tool for therapeutic gene delivery. However, manufacturing enough rAAV products remains a bottleneck in the industry. In the past decade, various production platforms have been developed with improvements in productivity, but limitations still exist.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Nandakumar Vidya, Krishnagowdu Saravanan, Jayachandran Halka, Kumaresan Kowsalya, Jaganathan Sakthi Yazhini Preetha, Packiaraj Gurusaravanan, Ramalingam Radhakrishnan, A. Usha Raja Nanthini, Muthukrishnan Arun
Summary: Isoflavones are potent secondary metabolites widely distributed among Fabaceae family, with beneficial functions such as lowering cardiovascular diseases and reducing menopausal symptoms. In vitro studies have successfully synthesized value-added metabolites with industrial applications, while plant organ cultures are recommended for synthesizing plant-based pharmaceutically active isoflavones. Biotechnological approaches can promote the yield and production of these metabolites.
PLANT BIOTECHNOLOGY REPORTS
(2021)
Article
Plant Sciences
Nora Gampe, Zoltan Szakacs, Andras Darcsi, Imre Boldizsar, Eva Szoke, Inna Kuzovkina, Laszlo Kursinszki, Szabolcs Beni
Summary: Hairy root cultures of Ononis spinosa and Ononis arvensis were established and analyzed for their isoflavonoid profiles, showing significant differences from wild-grown samples with the most abundant compounds being medicarpin glucoside and sativanone glucoside. The overall isoflavonoid production of the cultures was comparable to wild-grown O. arvensis and approximately twice as high as in wild-grown O. spinosa samples.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Materials Science, Biomaterials
Md Arif Khan, Madeleine Fugate, Dennis T. Rogers, Jatinder Sambi, John M. Littleton, Stephen E. Rankin, Barbara L. Knutson
Summary: The uptake and expulsion mechanisms of engineered mesoporous silica nanoparticles (MSNPs) in hairy roots were examined, showing that functionalization and surface charge play a key role in their effectiveness. Weakly charged MSNPs functionalized only with Ti were taken up and expelled by thermally activated mechanisms, while amine-modified positively charged particles were mainly taken up and expelled through direct penetration of cell walls. Amine-functionalized MSNPs moved spontaneously in and out of plant cells through dynamic exchange, suggesting potential as a biomolecule nanoharvesting platform for plant organ cultures.
Review
Food Science & Technology
Tanmay Sarkar, Molla Salauddin, Arpita Roy, Nikita Sharma, Apoorva Sharma, Saanya Yadav, Vaishnavi Jha, Maksim Rebezov, Mars Khayrullin, Muthu Thiruvengadam, Ill-Min Chung, Mohammad Ali Shariati, Jesus Simal-Gandara
Summary: This article introduces the definition and classification of tropical fruits, as well as their role in food fortification. It provides an overview of the nutritional composition, phytochemical composition, health benefits, and traditional uses of 26 minor tropical fruits.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Integrative & Complementary Medicine
Arpita Roy, Ashutosh Anand, Saksham Garg, Mohd Shahnawaz Khan, Sidharth Bhasin, Muhammad Nadeem Asghar, Talha Bin Emran
Summary: The study selected compounds from medicinal plants and proteins associated with breast cancer for screening and analysis. Several potential lead compounds were identified for their anti-breast cancer effects. Further in vitro studies are needed to validate the efficacy of these compounds.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Review
Integrative & Complementary Medicine
Arka Jyoti Chakraborty, Tanvir Mahtab Uddin, B. M. Redwan Matin Zidan, Saikat Mitra, Rajib Das, Firzan Nainu, Kuldeep Dhama, Arpita Roy, Md. Jamal Hossain, Ameer Khusro, Talha Bin Emran
Summary: This review article discusses the various therapeutic and pharmacological effects of onions, including anti-cancer, anti-diabetic, antioxidant, and hypotensive effects. Onions also have beneficial effects on the digestive, circulatory, and respiratory systems, as well as on the immune system.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Nanoscience & Nanotechnology
Virendra Kumar Yadav, G. Gnanamoorthy, Daoud Ali, Sweta Parimita Bera, Arpita Roy, Gokhlesh Kumar, Nisha Choudhary, Haresh Kalasariya, Anup Basnet
Summary: Magnetic nanoparticles have wide applications in medicine, electronics, and environmental cleanup. This study synthesized amorphous iron oxide nanoparticles (AIONPs) from waste incense sticks ash (ISA) in two steps. The results showed that AIONPs induced cell toxicity and had particle aggregation. Analysis methods such as Fourier transform infrared and Raman spectroscopy confirmed the chemical composition and magnetic strength of the synthesized AIONPs. Finally, the AIONPs were used for the removal of dye from wastewater, achieving a removal rate of 72%.
JOURNAL OF NANOMATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Arpita Roy, Vishwajeet Singh, Sukriti Sharma, Daoud Ali, Abul Kalam Azad, Gokhlesh Kumar, Talha Bin Emran
Summary: Synthesis of nanoparticles using plant extract, such as Catharanthus roseus, offers an efficient, cost-effective, and environmentally friendly method. The synthesized iron nanoparticles from Catharanthus roseus exhibit antibacterial activity against E. coli and dye degradation activity against methyl orange dye.
JOURNAL OF NANOMATERIALS
(2022)
Article
Chemistry, Physical
Komal Rizwan, Muhammad Bilal, Yassine Slimani, Pau Loke Show, Sami Rtimi, Arpita Roy, Hafiz M. N. Iqbal
Summary: Increasing global concern over hazardous pollutants in water bodies due to their toxicity and threat to the environment has led to the need for new or upgraded remediation methods. Advanced oxidation processes (AOPs) utilizing highly reactive oxidizing radicals like center dot O and center dot OH have shown efficacy in the mineralization of dye pollutants. Sonolysis, a newer AOP that employs ultrasound irradiation for generating oxidizing radicals, has become a robust hybrid AOP for degrading environmental contaminants. This review focuses on the degradation of dyes through ultrasound-based AOPs and discusses different sono-based methods and their efficacy for wastewater treatment.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Integrative & Complementary Medicine
Farhana Alam Ripa, Md. Jamal Hossain, Mst. Luthfun Nesa, Miss Sharmin Zahan, Saikat Mitra, Mohammad A. Rashid, Arpita Roy, Saad Alghamdi, Mazen Almehmadi, Osama Abdulaziz
Summary: This study evaluated the in vitro pharmacological properties of different solvent fractions of the ethanolic extract of Heritiera fomes Buch. Ham., including thrombolytic, antiarthritic, anthelmintic, and insecticidal activities. The findings confirmed the potential therapeutic effects of the plant and highlighted the need for further research on the isolation and identification of active compounds for new drug development.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Review
Chemistry, Physical
Arpita Roy, Madhura Roy, Saad Alghamdi, Anas S. Dablool, Angham Ahmed Almakki, Ismat H. Ali, Krishna Kumar Yadav, Md. Rabiul Islam, Marina M. S. Cabral-Pinto
Summary: Pesticides are toxic chemicals that pose threats to aquatic organisms and human health. Conventional methods for pesticide removal have limitations, and alternative approaches are needed.
INTERNATIONAL JOURNAL OF PHOTOENERGY
(2022)
Article
Nanoscience & Nanotechnology
Syed Taifa, Amatul Muhee, Riyaz A. Bhat, Showkat U. Nabi, Arpita Roy, Gulzar Ahmed Rather, Azmat Alam Khan, Showkeen Muzamil Bashir, Mohsina Patwekar, Shadma Wahab, Saad Alghamdi, Anas S. Dablool, Ahmed Kabrah, Umme Hani, Azzah M. Bannunah, Faheem Patwekar, Jewel Mallick
Summary: The current study aimed to develop alternative, nonantibiotic, and economically viable treatments for bovine mastitis. The results showed that treatment with CuNPs at a concentration of 6.25 mu g/mL can improve clinical signs, reduce bacterial load and oxidative stress indices, and result in significant improvement in histopathological changes in mammary glands.
JOURNAL OF NANOMATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Arifa Sultana, Md Jamal Hossain, Md Ruhul Kuddus, Mohammad A. Rashid, Miss Sharmin Zahan, Saikat Mitra, Arpita Roy, Safaet Alam, Md Moklesur Rahman Sarker, Isa Naina Mohamed
Summary: This overview summarizes the pharmacological properties of Euphorbia neriifolia and its derived phytochemicals against bacterial, viral, fungal, and parasitic infections. The results indicate that this plant and its chemical compounds exhibit antimicrobial activities against various pathogens, suggesting their potential as lead compounds for the treatment of emerging infectious diseases.
Review
Chemistry, Physical
Arpita Roy, Srijal Kunwar, Utsav Bhusal, Saad Alghamdi, Mazen Almehmadi, Hayaa M. Alhuthali, Mamdouh Allahyani, Md. Jamal Hossain, Md. Abir Hasan, Md. Moklesur Rahman Sarker, Mohd Fahami Nur Azlina
Summary: Nanoparticles less than 100 nm in size have attracted considerable interest in various fields due to their unique physical and chemical characteristics. Trimetallic nanoparticles have shown superior catalytic, biomedical, antimicrobial, active food packaging, and sensing applications compared to monometallic or bimetallic nanoparticles. However, the chemical and physical methods currently used for synthesizing these nanoparticles are expensive and environmentally toxic. This review provides an overview of the synthesis of trimetallic nanoparticles using biological agents and explores their applications in areas such as anticancer, antimicrobial activity, drug delivery, and catalytic activity.
Article
Chemistry, Physical
Srijal Kunwar, Arpita Roy, Utsav Bhusal, Amel Gacem, Mahmood M. S. Abdullah, Promila Sharma, Krishna Kumar Yadav, Sarvesh Rustagi, Nidhi Chatterjee, Vishal Kumar Deshwal, Hyun-Kyung Park, Byong-Hun Jeon
Summary: In this study, a greener, safe, reliable, and eco-friendly approach using an aqueous leaf extract of Catharanthus roseus was employed for the biological synthesis of Cu, Ag, Zn nanoparticles. The nanoparticles were characterised using various techniques, including UV-visible spectroscopy, XRD, FTIR, and EDX analysis. The synthesised trimetallic nanoparticles exhibited antioxidant and catalytic dye degradation activities.
Review
Crystallography
Dahir Sagir Idris, Arpita Roy
Summary: Nanotechnology has seen a surge in research interest in manipulating matter at the atomic level. Bimetallic nanoparticles have gained importance due to their high biocompatibility, stability, and low toxicity. This article explores and explains the various synthesis methods, including physical, chemical, and biological approaches, and discusses their advantages. Bimetallic nanoparticles have wide-ranging applications in medicine, agriculture, water treatment, nanosensors, nanochips, nano-semiconductors, optics, catalysis, and paints. While the green synthesis approach shows promise in producing more biocompatible and less toxic nanoparticles, there is still a need to address their potential toxicity and hazardous effects.
Article
Chemistry, Physical
Yogeshwar Baste, Vikram Jadhav, Arpita Roy, Saad Alghamdi, Mohamed Abbas, Jari S. Algethami, Mazen Almehmadi, Mamdouh Allahyani, Devvret Verma, Krishna Kumar Yadav, Byong-Hun Jeon, Hyun-Kyung Park
Summary: The synthesis of silver-incorporated copper oxide nanomaterials (Ag@CuO NMs) using a polyol-mediated refluxing method is discussed in this study. The photocatalytic activity of these nanomaterials, specifically their ability to degrade methylene blue (MB) dye, is investigated. Results show that the NMs with 5% weight of silver exhibited the highest degradation activity against MB dye, indicating their potential for wastewater purification.
Article
Chemistry, Physical
Sunil Chauhan, Shweta Rai, Soumya Pandit, Arpita Roy, Amel Gacem, Gamal A. El-Hiti, Krishna Kumar Yadav, Balasubramani Ravindran, Ji-Kwang Cheon, Byong-Hun Jeon
Summary: The Microbial Desalination Cell (MDC) is a new method for desalinating water and generating energy. The main issue with the technology is the low power generation during the oxygen reduction reaction (ORR). In this study, neodymium-doped zinc oxide nanoparticles were used as a highly active cathode catalyst to improve the performance of the MDC. The Nd-doped ZnO catalyst showed higher desalination efficiency and power density compared to the MDC without a catalyst.
