Review
Virology
Boris Ryabchenko, Vojtech Sroller, Lenka Hornikova, Alexey Lovtsov, Jitka Forstova, Sandra Huerfano
Summary: This review provides detailed descriptions of the diverse and complex interactions between small and medium size DNA viruses and promyelocytic leukemia nuclear bodies (PM NBs). The interactions between PM NB components and viruses can influence viral genome expression and/or replication, as well as IFN-mediated or apoptotic cell responses to viral infection. The review also discusses the interactions between PM NBs and Hepatitis B virus, papillomaviruses, polyomaviruses, and avian anellovirus. Despite numerous studies on the functions of PM NBs in the context of viral infection, there are still gaps in our understanding of the fine interactions between viruses and the highly dynamic PM NBs, which require further investigation.
Article
Biology
Myriam Scherer, Clarissa Read, Gregor Neusser, Christine Kranz, Anna K. Kuderna, Regina Mueller, Florian Full, Sonja Woerz, Anna Reichel, Eva-Maria Schilling, Paul Walther, Thomas Stamminger
Summary: PML nuclear bodies undergo rearrangement to form giant cages upon viral infection, trapping viral genomes and capsids, and providing a multilayered defense strategy against viral infections.
Review
Cell Biology
James Budzak, Gloria Rudenko
Summary: The African trypanosome Trypanosoma brucei has a dense layer of Variant Surface Glycoprotein (VSG) coat that protects it from immune attack. High levels of RNA transcription and pre-mRNA processing, as well as chromatin rearrangement, are important for the abundant expression of VSG.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Seishiro Hirano, Osamu Udagawa
Summary: Promyelocytic leukemia (PML) proteins play a role in the development of acute promyelocytic leukemia (APL) and can be affected by trivalent arsenic (As3+). The exposure to As3+ can lead to changes in PML-NBs, including solubility shift, SUMOylation, and late agglomeration. Understanding these changes is important for the regulation of intranuclear dynamics of PML-NBs.
Review
Virology
Behdokht Jan Fada, Eleazar Reward, Haidong Gu
Summary: ND10, also known as PML-NBs, are dynamic membraneless subnuclear domains involved in key cellular processes such as DNA damage response, transcription regulation, apoptosis, oncogenesis, and antiviral defenses.
Article
Biochemistry & Molecular Biology
Hongyao Sun, Yutong Chen, Kun Yan, Yanqiu Shao, Qiangfeng C. Zhang, Yi Lin, Qiaoran Xi
Summary: TRIM33 co-localizes with PML-NBs in mESCs to mediate Nodal signaling-directed transcription of Lefty1/2. Formation of TRIM33 puncta depends on PML and specific assembly of PML-NBs. PML and PML-NBs regulate TRIM33 recruitment and association with Lefty1/2 loci in mESCs.
Article
Virology
Linda Schweiger, Laura A. Lelieveld-Fast, Snjezana Mikulicic, Johannes Strunk, Kirsten Freitag, Stefan Tenzer, Albrecht M. Clement, Luise Florin
Summary: Human papillomaviruses (HPVs) cause significant burden on human population, leading to various cancers. The autophagy adaptor p62 is identified as a novel proviral factor in HPV16 infection. p62 interacts with virus-filled endosomes, accompanies the virus to the sites of viral transcription and replication, and plays a crucial role in HPV16 infection. Depletion of p62 enhances the targeting of viral components to autophagic structures and degradation of viral capsid protein.
Review
Chemistry, Multidisciplinary
Fangzhou Li, Xinmiao Lan, Xing-Jie Liang
Summary: Most cellular elements function by forming aggregate structures through interactions with other molecules, with the aggregation of biological elements being more complex compared to AIE molecules, following the trend in biological evolution from simplicity to complexity. The protein-protein complex (PML-NB), protein-nucleic acid complex ribosome, and protein-nucleic acid-lipid complex exosome represent three types of units that perform functions within cells.
Article
Immunology
Songbai Yang, Huaijin Liu, Zhenyu Chen, Han Wang, Xiangchen Li, Xiaolong Zhou, Ayong Zhao
Summary: This study investigates how Japanese encephalitis virus (JEV) evades immunity by disrupting the structure of PML-nuclear bodies (PML-NBs) through interaction with PML isoforms, potentially attenuating the host's antiviral immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biology
Isaac Armendariz-Castillo, Katherine Hidalgo-Fernandez, Andy Perez-Villa, Jennyfer M. Garcia-Cardenas, Andres Lopez-Cortes, Santiago Guerrero
Summary: APBs are a hallmark of telomere maintenance in cancer cells, and have the potential to serve as therapeutic markers.
Article
Cell Biology
Jaemin Eom, Kyuheum Jeon, Jung Sun Park, Yong-Kook Kang
Summary: SETDB1 is a histone methyltransferase that can be found in both the nucleus and cytoplasm. Its localization is primarily regulated by a balance between nuclear localization signals (NLS) and nuclear export signals (NES). Research findings suggest that cytoplasmic localization is a result of weak NLS and strong NES. In the presence of ATF7IP, SETDB1 enters the nucleus through a specific NES motif. The interaction between SETDB1 and ATF7IP in the nucleus helps maintain balanced levels of SETDB1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Toyoshi Fujimoto
Summary: Lipid droplets in the cytoplasm are formed in the endoplasmic reticulum and are connected with various organelles. Nuclear lipid droplets in hepatocytes are derived from lipoprotein precursors in the ER lumen, while in non-hepatocytes and budding yeast, they are newly formed in the inner nuclear membrane. Although nuclear lipid droplets are fewer in number, their unique location suggests specific functions related to nuclear biology.
JOURNAL OF CELL SCIENCE
(2022)
Editorial Material
Biochemistry & Molecular Biology
Xiaodong Cheng
Summary: Protein SUMOylation is an essential post-translational modification for maintaining cellular homeostasis. Recent evidence suggests that liquid-liquid phase separation (LLPS)/ biomolecular condensates play a role in regulating cellular SUMOylation, providing insights into its cellular mechanism.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sven Seitz, Anna Theresa Heusel, Thomas Stamminger, Myriam Scherer
Summary: PML nuclear bodies (PML-NBs) play a cell type-specific antiviral role against HCMV infection, with PML and Daxx having contrasting effects at different stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Samuel Hofmann, Miona Stubbe, Julia Mai, Sabrina Schreiner
Summary: PML nuclear bodies play a crucial role in virus infections by accumulating SUMO-dependent factors, which are targeted and manipulated by adenoviral proteins. Despite their antiviral reputation, Human Adenoviruses strategically utilize PML nuclear bodies for productive infection and oncogenic transformation, potentially counteracting antiviral responses while benefiting from or even depending on them.
Article
Biochemistry & Molecular Biology
Ruimeng Yang, Ming Zhan, Miaomiao Guo, Hao Yuan, Yiqin Wang, Yiyue Zhang, Wenqing Zhang, Saijuan Chen, Hugues de The, Zhu Chen, Jun Zhou, Jun Zhu
Summary: Microglia, the primary immune cells in the central nervous system, are regulated by the cerebral white matter hyperintensities susceptibility gene PDCD11, which plays a crucial role in microglia differentiation during zebrafish development.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Immunology
Tracy Dagher, Nabih Maslah, Valerie Edmond, Bruno Cassinat, William Vainchenker, Stephane Giraudier, Florence Pasquier, Emmanuelle Verger, Michiko Niwa-Kawakita, Valerie Lallemand-Breitenbach, Isabelle Plo, Jean-Jacques Kiladjian, Jean-Luc Villeval, Hugues de The
Summary: Research shows that arsenic trioxide (ATO) can significantly enhance IFNα-induced growth suppression of JAK2 (V617F) patient or mouse hematopoietic progenitors, predicting potent clinical efficacy of the IFNα+ATO combination in patients. This combination therapy has the potential to eradicate Myeloproliferative neoplasms by targeting disease-initiating cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Hematology
Marie-Claude Geoffroy, Cecile Esnault, Hugues de The
Summary: Retinoic acid receptors play crucial roles in leukemia, with recent studies showing their significance in non-APL AML and normal hematopoietic differentiation. This sheds light on the potential broader uses of retinoids in the management of hematopoietic tumors.
Article
Hematology
Hiba El Hajj, Rita Hleihel, Marwan El Sabban, Julie Bruneau, Ghazi Zaatari, Morgane Cheminant, Ambroise Marcais, Abdou Akkouche, Hideki Hasegawa, William Hall, Hugues De The, Olivier Hermine, Ali Bazarbachi
Summary: Combining arsenic trioxide and interferon-alpha shows high response rate in chronic ATL patients by shutting down IL-10 expression to eliminate leukemia-initiating cell activity, leading to immune-mediated clearance of leukemic cells.
Editorial Material
Hematology
Fang Qiu, Hugues De The
Article
Oncology
Simona Moretti, Amal Kamal Abdel-Aziz, Elena Ceccacci, Isabella Pallavicini, Fabio Santoro, Hugues de The, Saverio Minucci
Summary: This study demonstrates the importance of simultaneously targeting the plasticity and heterogeneity of cancer cells, specifically leukemia-initiating cells (LICs), in achieving and maintaining complete remission (CR) in acute myeloid leukemia (AML). The combination of certain histone deacetylase (HDAC) inhibitors and all-trans retinoic acid (ATRA) effectively differentiated and targeted LICs, leading to CR in a murine model of AML.
Article
Oncology
Bryann Pardieu, Justine Pasanisi, Frank Ling, Reinaldo Dal Bello, Justine Penneroux, Angela Su, Romane Joudinaud, Laureen Chat, Hsin Chieh Wu, Matthieu Duchmann, Gaetano Sodaro, Clementine Chauvel, Florence A. Castelli, Loic Vasseur, Kim Pacchiardi, Yannis Belloucif, Marie-Charlotte Laiguillon, Eshwar Meduri, Camille Vaganay, Gabriela Alexe, Jeannig Berrou, Chaima Benaksas, Antoine Forget, Thorsten Braun, Claude Gardin, Emmanuel Raffoux, Emmanuelle Clappier, Lionel Ades, Hugues de The, Francois Fenaille, Brian J. Huntly, Kimberly Stegmaier, Herve Dombret, Nina Fenouille, Camille Lobry, Alexandre Puissant, Raphael Itzykson
Summary: By analyzing multiple AML datasets, we identified SLC7A11 as a potential gene that is essential for AML cell survival. Inhibition of SLC7A11 using genetic and chemical methods reduced the viability and clonogenic capacity of AML cell lines. Sulfasalazine, a drug with xCT inhibitory activity, showed anti-leukemic effects against primary AML samples in vitro. Inhibition of xCT affected multiple metabolic pathways, leading to depletion of glutathione pools and oxidative stress-induced cell death in leukemic cells.
Review
Cell Biology
Umut Sahin, Hugues de The, Valerie Lallemand-Breitenbach
Summary: Sumoylation is an important post-translational modification that regulates intricate networks within eukaryotic cells. It plays a key role in transcription, nuclear integrity, proliferation, senescence, lineage commitment, and stemness. Additionally, it is involved in infection, immune processes, epigenetic processes, and development. It has been associated with various pathological conditions and has therapeutic potential.
Editorial Material
Hematology
Hsin-Chieh Wu, Hugues de The
Summary: This study investigates the impact of focal noncoding enhancer mutations on MYB binding and WT1 expression in acute promyelocytic leukemia (APL) and reveals their resemblance to a germline single nucleotide polymorphism (SNP) that promotes APL emergence.
Article
Multidisciplinary Sciences
Sarah Tessier, Omar Ferhi, Marie-Claude Geoffroy, Roman Gonzalez-Prieto, Antoine Canat, Samuel Quentin, Marika Pla, Michiko Niwa-Kawakita, Pierre Bercier, Domitille Rerolle, Pierre Therizols, Emmanuelle Fabre, Alfred C. O. Vertegaal, Hugues de The, Valerie Lallemand-Breitenbach
Summary: The authors identify novel PML partners and demonstrate their role in controlling sumoylation and maintaining the transcriptome and transposable element silencing in embryonic stem cells. These findings shed light on the functions of membrane-less organelles and provide new insights into the potential role of PML in embryonic stem cells and cancer.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Yun Deng, Haihong Wang, Xiaohui Liu, Hao Yuan, Jin Xu, Hugues de The, Jun Zhou, Zhu Jun, Florent Ginhoux
Summary: This study discovers that the deficiency of ZBTB14 gene leads to an abnormal expansion of monocyte/macrophage population in zebrafish. ZBTB14 functions as a negative regulator of PU.1, and the repression activity of ZBTB14 depends on SUMOylation on a conserved lysine. Furthermore, a mutation can target ZBTB14 protein to autophagic degradation.
Correction
Multidisciplinary Sciences
Sarah Tessier, Omar Ferhi, Marie-Claude Geoffroy, Roman Gonzalez-Prieto, Antoine Canat, Samuel Quentin, Marika Pla, Michiko Niwa-Kawakita, Pierre Bercier, Domitille Rerolle, Marilyn Tirard, Pierre Therizols, Emmanuelle Fabre, Alfred C. O. Vertegaal, Hugues de The, Valerie Lallemand-Breitenbach
NATURE COMMUNICATIONS
(2023)
Review
Immunology
Domitille Rerolle, Hugues de The
Summary: PML nuclear bodies are stress-responsive domains involved in senescence, apoptosis, or metabolism. PML is required for response or even cure of some hematological malignancies. The authors review how PML participates in cancer cell clearance and highlight its druggable aspect.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
