Article
Cell Biology
Rachel L. Flores, Zachary E. Peterson, Alex Zelter, Michael Riffle, Charles L. Asbury, Trisha N. Davis
Summary: The study reveals that interactions between Ndc80 complex and Dam1 complex through three distinct interacting regions play a vital role in supporting both short-term and long-term tip-coupling during mitosis. Phosphorylation by Aurora B kinase may modulate these interactions and impact the accuracy of mitosis.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ayumu Yamamoto
Summary: Accurate chromosome segregation is crucial for cell proliferation and sexual reproduction. While the mechanisms of attachment selection and elimination are not fully understood, recent studies suggest that chromosome oscillation may play a role in the elimination of erroneous attachments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Harinath Doodhi, Tomoyuki U. Tanaka
Summary: This article discusses recent findings in budding yeast regarding the molecular mechanisms involved in the error correction process during chromosome segregation in mitosis. The study reveals that Aurora B kinase plays a crucial role in regulating the exchange and cessation of kinetochore-microtubule interactions to resolve aberrant interactions, ultimately ensuring proper chromosome biorientation.
Review
Oncology
Kenji Iemura, Yujiro Yoshizaki, Kinue Kuniyasu, Kozo Tanaka
Summary: Chromosomal instability (CIN) is commonly observed in cancer cells, associated with tumor progression and poor prognosis. Insufficient correction of erroneous kinetochore-microtubule attachments plays pivotal roles in CIN, with chromosome oscillation promoting error correction by phosphorylating Hec1 through Aurora A kinase. Understanding the link between attenuated chromosome oscillation and CIN in cancer cells may provide potential targets for cancer therapy.
Article
Multidisciplinary Sciences
Laras Pitayu-Nugroho, Melanie Aubry, Kimberley Laband, Helene Geoffroy, Thadshagine Ganeswaran, Audi Primadhanty, Julie C. Canman, Julien Dumont
Summary: During oocyte meiosis, the distribution, orientation, and alignment of chromosomes in the spindle are crucial for accurate segregation. This study used live imaging to investigate the role of kinetochore proteins in C. elegans oocytes and proposed a push-pull mechanism involving the BHC module and Ndc80 complex for accurate chromosome segregation. The findings underscore the importance of different kinetochore components in promoting correct holocentric chromosome segregation in oocytes of C. elegans.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Babhrubahan Roy, Simon J. Y. Han, Adrienne N. Fontan, Soubhagyalaxmi Jema, Ajit P. Joglekar
Summary: Accurate chromosome segregation during cell division requires proper attachment between the spindle apparatus and the chromosomes. This is ensured by the combined activity of the spindle assembly checkpoint (SAC) and an error correction mechanism. Research has shown that Aurora B kinase directly promotes the production of the mitotic checkpoint complex (MCC) and works downstream of Mps1 in promoting SAC signaling.
Review
Cell Biology
Tomoyuki U. Tanaka, Tongli Zhang
Summary: This article discusses how eukaryotic cells ensure correct chromosome segregation during mitosis, focusing on the mechanisms of error correction through the SWAP, SWITCH, and STABILIZE processes. Key players in this process include Aurora B kinase, Mps1 kinase, and Stu2 microtubule polymerase. The study mainly examines mechanisms in budding yeast, where error correction mechanisms are relatively simpler due to a single microtubule attachment at biorientation.
Article
Biochemical Research Methods
Evgenii Kliuchnikov, Artem Zhmurov, Kenneth A. Marx, Alex Mogilner, Valeri Barsegov
Summary: This article introduces a Stochastic Reaction-Diffusion-Dynamics Model for simulations of cellular mechanochemical processes with high spatial and temporal resolution. The model was applied to test the Search-and-Capture mechanism of mitotic spindle assembly, revealing factors that improve the accuracy of chromosome connections.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Cell Biology
Luciano Gama Braga, Diogjena Katerina Prifti, Chantal Garand, Pawan Kumar Saini, Sabine Elowe
Summary: Accurate chromosome alignment during metaphase is crucial for proper chromosome segregation in cell division, and tools to measure alignment errors are important for understanding chromosome segregation defects. This study developed and validated a method to quantify chromosome misalignment, which will allow sensitive and systematic analysis of chromosome segregation defects in mitotic cells.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Cell Biology
Thomas J. Kucharski, Rufus Hards, Sarah E. Vandal, Maria Alba Abad, A. Arockia Jeyaprakash, Edward Kaye, Aymen Al-Rawi, Tony Ly, Kristina M. Godek, Scott A. Gerber, Duane A. Compton
Summary: Phosphorylation of kinetochore proteins plays a crucial role in correcting erroneous microtubule attachments during cell division. Through quantitative mass spectrometry, it was found that the phosphorylation of the kinetochore protein Hec1 is only partially occupied and showed minimal changes in response to microtubule attachment status. Both Cdk1-Cyclin B1 and Aurora kinases phosphorylate Hec1 to enhance error correction. The low level of phosphorylation promotes microtubule attachment to kinetochores, while the high sensitivity of kinetochore-microtubule attachments to changes in phosphorylation drives error correction and ensures accurate chromosome segregation.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Bernardo Orr, Filipe De Sousa, Ana Margarida Gomes, Olga Afonso, Luisa T. Ferreira, Ana C. Figueiredo, Helder Maiato
Summary: Aurora B kinase plays a crucial role in human cell division by actively monitoring and correcting frequent chromosome segregation errors to prevent micronuclei formation.
Review
Cell Biology
Andrew D. McAinsh, Geert J. P. L. Kops
Summary: The spindle assembly checkpoint (SAC) is crucial for correct chromosome segregation during cell division. It monitors the interaction of chromosomes with spindle microtubules and halts anaphase until all kinetochores are properly attached. Recent studies have provided insights into the dynamic signaling properties of SAC and its integration at mammalian kinetochores, which have multiple attachment points.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Review
Cell Biology
Helder Maiato, Sonia Silva
Summary: This article discusses the origin and fate of chromosome segregation errors that satisfy the spindle assembly checkpoint, focusing on anaphase surveillance/correction mechanisms and post-mitotic clearance pathways.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Oncology
Elizabeth A. Crowley, Nicole M. Hermance, Conor P. Herlihy, Amity L. Manning
Summary: Numerical chromosome instability (nCIN) is prevalent in solid tumors and is associated with aggressiveness, drug resistance, and relapse. This study shows that manipulating microtubule dynamics and enhancing chromosome cohesion can suppress nCIN and reduce intratumor heterogeneity in non-small cell lung cancer. The suppression of nCIN alters the timing and molecular mechanisms of acquired drug resistance.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Cell Biology
Vikash Verma, Thomas J. Maresca
Summary: This article reviews the current understanding of the formation of a bipolar spindle and discusses the self-organizing process involved. The potential future research directions in understanding the bipolar spindle are also explored.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Sandra A. Touati, Eulalie Buffin, Damien Cladiere, Khaled Hached, Christophe Rachez, Jan M. van Deursen, Katja Wassmann
NATURE COMMUNICATIONS
(2015)
Article
Biochemistry & Molecular Biology
Thierry Delaveau, Dimitri Davoine, Ariane Jolly, Antoine Vallot, Jerome O. Rouviere, Athenais Gerber, Sandra Brochet, Marion Plessis, Roxane Roquigny, Jawad Merhej, Thibaut Leger, Camille Garcia, Gaelle Lelandais, Elodie Laine, Benoit Palancade, Frederic Devaux, Mathilde Garcia
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Warif El Yakoubi, Eulalie Buffin, Damien Cladiere, Yulia Gryaznova, Ines Berenguer, Sandra A. Touati, Rocio Gomez, Jose A. Suja, Jan M. van Deursen, Katja Wassmann
NATURE COMMUNICATIONS
(2017)
Article
Developmental Biology
Khaled Hached, Stephanie Z. Xie, Eulalie Buffin, Damien Cladiere, Christophe Rachez, Marina Sacras, Peter K. Sorger, Katja Wassmann
Article
Cell Biology
Aude Dupre, Eulalie Buffin, Chloe Roustan, Angus C. Nairn, Catherine Jessus, Olivier Haccard
JOURNAL OF CELL SCIENCE
(2013)
Correction
Cell Biology
Aude Dupre, Eulalie Buffin, Chloe Roustan, Angus C. Nairn, Catherine Jessus, Olivier Haccard
JOURNAL OF CELL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Eulalie Buffin, Michel Gho
Review
Cell Biology
Antoine Vallot, Kikue Tachibana
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Yulia Gryaznova, Leonor Keating, Sandra A. Touati, Damien Cladiere, Warif El Yakoubi, Eulalie Buffin, Katja Wassmann
Summary: This research explores the separation of sister kinetochores and removal of cohesin during meiosis, revealing that the initial separation of sister kinetochores actually occurs during meiosis I, not meiosis II as previously thought. This indicates that whether centromeric cohesin is removed or not is determined by the kinetochore structure prior to meiosis II.
Article
Biochemistry & Molecular Biology
Elvira Nikalayevich, Safia El Jailani, Aude Dupre, Damien Cladiere, Yulia Gryaznova, Celia Fosse, Eulalie Buffin, Sandra A. Touati, Katja Wassmann
Summary: To separate chromosomes and sister chromatids in meiosis, cohesin needs to be removed from chromosome arms and centromere region. While phosphorylation of Rec8 is known to be necessary for its cleavage in yeast and C. elegans, it remains unclear whether this holds true in mammals. This study used live imaging and phosphomutant analysis to show that phosphorylation of Rec8 by Aurora B/C kinases is required for its cleavage in mammalian meiosis.
Article
Cell Biology
Eulalie Buffin, Doruk Emre, Roger E. Karess
NATURE CELL BIOLOGY
(2007)
Article
Biochemistry & Molecular Biology
E Buffin, C Lefebvre, JY Huang, ME Gagou, RE Karess