Article
Chemistry, Multidisciplinary
You Tian, Da-Wen Sun, Liang Xu, Tian-Hao Fan, Shu-Ti Zhang, Zhiwei Zhu
Summary: This study investigated the use of natural deep eutectic solvents (NADESs) as cryoprotectants for cytotoxicity-free and green cryopreservation. The results showed that NADESs could be safe and biocompatible, with excellent cryoprotective efficiency. Furthermore, NADES-cryopreserved cells exhibited better morphology and lower apoptosis compared to cells cryopreserved with glycerol and DMSO.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Physical
Aditi Prabhune, Ranjan Dey
Summary: Deep Eutectic Solvents (DESs) have emerged as potential alternatives to conventional solvents in many industrial applications due to their safety, greenness, ease of preparation and wide range of applications.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Multidisciplinary
Maninder Meenu, Vasudha Bansal, Sudha Rana, Nitya Sharma, Vinod Kumar, Vaishali Arora, Monika Garg
Summary: Anthocyanins are extracted using alternative solvents such as ionic liquids, deep eutectic solvents, and natural deep eutectic solvents, which are more environmentally friendly and efficient compared to conventional solvents. Acid-based deep eutectic solvents with 20-30% water content showed the highest anthocyanin extraction yield. Ultrasonic-assisted extraction was the most common and efficient method, and DES-based extracts exhibited the highest stability and antioxidant activity. Fruits and their by-products are the most commonly used samples, while cereal and legumes are less explored.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Biotechnology & Applied Microbiology
Fabian Thomas, Oliver Kayser
Summary: This study demonstrates the advantages of performing cannabinoid biotransformations in natural deep eutectic solvents (NADES), which significantly increase cannabinoid production. The research also suggests flexible manipulation of solvent compositions to enhance total cannabinoid content or alter cannabinoid specificity.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Chemical
Francesca Russo, Matteo Tiecco, Francesco Galiano, Raffaella Mancuso, Bartolo Gabriele, Alberto Figoli
Summary: In this study, porous polymeric membranes were prepared using DESs and NADESs for the first time, and the performance of the membranes was optimized by adding co-solvents and adjusting the composition of the coagulation bath. The results demonstrated the potential of DESs and NADESs as solvents for the preparation of sustainable polymeric membranes for water treatment applications.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Engineering, Environmental
Maria F. Nava-Ocampo, Lamya Al Fuhaid, Robert Verpoorte, Young Hae Choi, Mark C. M. van Loosdrecht, Johannes S. Vrouwenvelder, Geert J. Witkamp, Andreia S. F. Farinha, Szilard S. Bucs
Summary: The study demonstrates that NADES can act as a biofilm structural breaker, solubilizing the main components of biofilms and weakening their structure, potentially serving as an environmentally-friendly green solvent for biofilm treatment.
Article
Chemistry, Physical
Helene Liepelt Nystedt, Krister Gjestvang Gronlien, Hanne Hjorth Tonnesen
Summary: Natural deep eutectic solvents (NADES) show promise as drug carriers in new drug delivery systems for topical applications, although further investigation into their effects in biological systems is needed.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Physical
Jose L. Trenzado, Cristina Benito, Mert Atilhan, Santiago Aparicio
Summary: This study investigated the hydrophobic Natural Deep Eutectic Solvent formed by cineole and decanoic acid through both experimental and computational approaches. The experimental study measured physicochemical properties and Raman spectra, confirming the formation of hydrogen bonding. The computational analysis using Density Functional Theory and Molecular Dynamics simulation provided nanoscopic characterization and predicted phase equilibria. Moreover, the interaction of the solvent with POPC lipid was studied, revealing its disruptive effects on cell membranes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Engineering, Chemical
Yunchang Fan, Hui Luo, Chunyan Zhu, Wenjin Li, Di Wu, Hongwei Wu
Summary: The hydrophobic natural alcohols based deep eutectic solvents (NADESs) synthesized in this work showed high extraction ability for quinine, with NADESs of lower viscosity and stronger hydrogen bond accepting ability exhibiting stronger extraction ability. Quinine acted as a hydrogen bond acceptor in the hydrogen bonding with NADESs, and the extraction efficiency of NADESs for quinine was higher than other solvents such as ionic liquids, trichloromethane, and ethyl acetate. The NADES phase could be reused for extraction, and quinine could be recovered from NADES phase via back extraction with 1.0% acetic acid aqueous solution, showing potential as promising solvents for extraction of bioactive compounds.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Chemistry, Analytical
Susanna Della Posta, Valeria Gallo, Alessandra Gentili, Chiara Fanali
Summary: The need to reduce the ecological impact of waste in the extraction and analysis processes has led to the use of alternative green solvents. Deep eutectic solvents (DESs) have shown superior extraction capabilities for bioactive molecules, but have difficulties in removal from extracted mixtures. This review provides an overview and critical evaluation of methods for recovering bioactive molecules from extracts obtained using DESs and natural deep eutectic solvents (NADESs).
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Jinhao Yao, Chunlu Li, Lei Xiao, Yingying Wu, Qianwen Wu, Zhifang Cui, Bo Wang
Summary: By studying the thermal stability and conformational changes of cellulase in different natural deep eutectic solvents (NADESs), green and suitable solvents were found to enhance the stability of cellulase, which is important for the utilization of lignocellulosic biomass.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Green & Sustainable Science & Technology
Benteng Liu, Zhaopeng Hu, Xinke Zhong, Li Yang, Xin Jiang, Min Zuo, Miao Du, Yihu Song, Qiang Zheng, Danling Wang
Summary: This study investigated the effects of deep eutectic solvents (DESs) on rubber vulcanization reactions and found that DES can participate in the formation of crosslinking networks and regulate the crosslinking density through interactions with rubber components and activators. Moreover, DES influences the rheological and mechanical properties of the vulcanizates, providing a way to produce high-strength rubber products at lower vulcanization temperatures.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Analytical
Frederik Andre Hansen, Elia Santigosa-Murillo, Maria Ramos-Payan, Maria Munoz, Elisabeth Leere Oiestad, Stig Pedersen-Bjergaard
Summary: This study investigated hydrophobic deep eutectic solvents as supported liquid membranes for electromembrane extraction, and found that mixtures of coumarin and thymol were efficient in extracting non-polar bases, non-polar acids, and polar bases. The SLMs with aromatic properties exhibited high hydrogen bonding capabilities, providing excellent clean-up efficiency with no matrix effects from plasma.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Ahmad Al-Bodour, Noor Alomari, Alberto Gutierrez, Santiago Aparicio, Mert Atilhan
Summary: This work investigates the solubility of carbon dioxide in four type V natural deep eutectic solvents (NADES). The results show that the solubility of carbon dioxide in monoterpenoid-based hydrophobic NADES increases with increasing pressure and decreasing temperature. In addition to their hydrophobic nature, these solvents have high CO2 sorption performance and rapid solubility rate, making them good candidates for future carbon management applications.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Physical
Tatjana Juric, Denis Uka, Berta Barta Hollo, Branislav Jovic, Branko Kordic, Boris M. Popovic
Summary: The properties of natural deep eutectic solvents depend on the structure of hydrogen bond donors (HBD) present in the solvents, while the thermal stability is influenced by the HBD structure. These solvents can be considered tailor-made, as their properties can be easily adjusted to suit different applications.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Engineering, Multidisciplinary
Tian Jiao, Qin Lian, Weilong Lian, Yonghui Wang, Dichen Li, Rui L. Reis, Joaquim Miguel Oliveira
Summary: This study examines the properties of Collagen/Sodium Alginate (Col/SA) hydrogels for the purpose of 3D printing skin. It found that increasing the sodium alginate content improves the mechanical and swelling properties of the hydrogel, but has negative effects on degradation ratio, pore size, and biological properties. A 1% collagen hydrogel was identified as the optimal bioink for printing skin, as it showed improved cell spreading and proliferation.
JOURNAL OF BIONIC ENGINEERING
(2023)
Review
Food Science & Technology
Diana I. Meira, Ana I. Barbosa, Joel Borges, Rui L. Reis, Vitor M. Correlo, Filipe Vaz
Summary: Global population growth has a significant impact on the global food industry, posing a threat to food safety and quality. Mycotoxins, particularly Ochratoxin-A (OTA), are produced by fungus that contaminates various food species and products. OTA not only endangers food production but also poses potential toxicological risks to humans, leading to carcinogenic and neurological diseases. Therefore, the development of a selective, sensitive, and reliable OTA biodetection approach is crucial for ensuring food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Multidisciplinary
Manuel Somoza, Ramon Rial, Zhen Liu, Iago F. Llovo, Rui L. Reis, Jesus Mosqueira, Juan M. Ruso
Summary: This study successfully created doped Hydroxyapatite nanoparticles with magnetic properties using microfluidics and Gadolinium as a contrast agent for medical applications. Computational Fluid Dynamics (CFD) was used to ensure the system worked in the laminar regime and nanoparticles diffused uniformly. The biomaterials were characterized using XRD, FE-SEM, EDX, confocal Raman microscopy, and FT-IR, confirming the successful incorporation of Gadolinium. Magnetic characterization confirmed the paramagnetic behavior of the nanoparticles, showing the potential for advanced nanomaterials in theragnostic applications.
Article
Oncology
Sara Monteiro-Reis, Joao P. S. Ferreira, Ricardo A. Pires, Joao Lobo, Joao A. Carvalho, Rui L. Reis, Renato Natal Jorge, Carmen Jeronimo
Summary: This study found that the presence of a tumor can alter the stiffness of the bladder wall, which may affect the progression of bladder cancer. Using atomic force microscopy, the researchers measured the micromechanical properties of the bladder wall layers and observed that tumors promote the development of muscle-like structures in the mucosa.
Article
Materials Science, Multidisciplinary
Xuelian Yin, Lin Zhan, Lin Ding, Xuerui Chen, Junfeng Zhang, Chenchen Li, Yuxi Zhang, Murad Khan, Rui L. Reis, Yanli Wang
Summary: We report a facile synthesis method for mitochondria-specific anchored nano-complexes (AG-C-NP) comprised of a skeleton of gold and carbon atoms for cancer therapy. Compared to gold nanoclusters (Au NCs), AG-C-NP showed enhanced fluorescence imaging effects, which can be used for tumor monitoring. AG-C-NP targeted the mitochondria and increased ROS damage in cancer cells, leading to a significant inhibition of tumor growth.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
Anita Akbarzadeh Solbu, David Caballero, Spyridon Damigos, Subhas C. Kundu, Rui L. Reis, Oyvind Halaas, Aman S. Chahal, Berit L. Strand
Summary: Cell migration plays a crucial role in various biological processes, and recent advancements in materials science have allowed for the development of biomimetic in vitro assays to study cell migration. Three-dimensional hydrogels have been particularly useful in mimicking the extracellular environment and studying cell migration in a more physiologically relevant manner. This article provides an overview of the different types of hydrogels and their characteristics, as well as established methods for cell migration assays and tracking in three-dimensional environments.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Physical
Andreas S. Croft, Slavko Corluka, Janine Fuhrer, Michael Woeltje, Joana Silva-Correia, Joaquim M. Oliveira, Georg F. Erbach, Rui L. Reis, Benjamin Gantenbein
Summary: Intervertebral disc herniation often causes severe pain and is associated with disc degeneration. We propose an annulus fibrosus repair approach using GG-MA and silk fibroin. The repaired discs showed no significant differences compared to the damaged discs, indicating the repair approach was successful.
Article
Polymer Science
Ines Machado, Catarina F. Marques, Eva Martins, Ana L. Alves, Rui L. Reis, Tiago H. Silva
Summary: Marine-origin gelatin, a safe alternative to mammalian-origin materials, has the benefits of structural similarity and low production cost. Gelatin-methacryloyl (GelMA)-based hydrogels have shown excellent capacity for biomedical applications, such as tissue engineering. In this study, fish gelatin was extracted from Greenland halibut skins and functionalized with methacrylation to develop a photosensitive GelMA. By combining GelMA with glycosaminoglycans (GAGs), biomechanically stable hydrogels with tunable physicochemical features were produced. These hybrid hydrogels, tested as a matrix for chondrocyte culture, showed improved cell viability and have potential for cartilage tissue engineering applications.
Article
Biotechnology & Applied Microbiology
Silvia Vieira, Paulina Strymecka, Luiza Stanaszek, Joana Silva-Correia, Katarzyna Drela, Michal Fiedorowicz, Izabela Malysz-Cymborska, Miroslaw Janowski, Rui Luis Reis, Barbara Lukomska, Piotr Walczak, Joaquim Miguel Oliveira
Summary: This study aims to develop a stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. By supplementing GG-MA solutions with paramagnetic Mn2+ ions, the hydrogel can be visualized under Magnetic Resonance Imaging (MRI). Cell-laden hydrogels prepared using the Mn/GG-MA formulations remained viable after 7 days of culture. In vivo tests on immunocompromised mice showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel visible on MRI scans. Overall, the developed formulations are suitable for non-invasive cell delivery techniques and image-guided neurointerventions.
BIOENGINEERING-BASEL
(2023)
Review
Chemistry, Multidisciplinary
Sara Gimondi, Helena Ferreira, Rui L. L. Reis, Nuno M. M. Neves
Summary: The use of nanoparticles in nanomedicine has great potential in overcoming the limitations of conventional therapies and improving early diagnosis and follow-up of diseases. Microfluidic systems offer an efficient and cost-effective method for nanoparticle synthesis and screening by simulating dynamic fluid flows and specific microenvironments. Microfluidic devices allow for controlled synthesis of nanoparticles, enhancing batch-to-batch reproducibility, and provide versatile platforms for rapid and efficient in vitro and in vivo screening. This review summarizes the major microfluidic platforms for controlled nanoparticle synthesis, discusses innovative platforms for mimicking in vitro environments and organism-on-a-chip, and evaluates the current challenges and future directions of microfluidic systems in nanomedicine.
Article
Engineering, Biomedical
David Caballero, Rui L. Reis, Subhas C. Kundu
Summary: Researchers have developed a cancer trap based on mechanical ratchet structures to capture metastatic cells. The trap exhibits high efficiency in capturing invasive cancer cells and may have therapeutic implications for cancer treatment and controlling cell motility in biological processes.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
Lara Pierantoni, Rui L. Reis, Joana Silva-Correia, Joaquim M. Oliveira, Susan Heavey
Summary: The fields of tissue bioengineering, -omics, and spatial biology offer the opportunity for a paradigm shift in breast cancer research. However, collaboration between these fields has not reached its full potential. This review discusses the latest 3D breast cancer models, their biomaterials and technological platforms, as well as their biological evaluation, highlighting the potential for better microphysiological systems, improved drug development success rates, and personalized medicine approaches.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Raul Fontelo, Rui L. Reis, Ramon Novoa-Carballal, Iva Pashkuleva
Summary: This paper summarizes the importance and development trends of block copolymer (BCP) self-assembly in nanofabrication, and discusses its potential and challenges in the field of bioapplications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biology
Temuulen Selenge, Sara F. Vieira, Odontuya Gendaram, Rui L. Reis, Soninkhishig Tsolmon, Enkhtuul Tsendeekhuu, Helena Ferreira, Nuno M. Neves
Summary: In this study, the ethanol and dichloromethane extracts of Stellera chamaejasme were evaluated for their antioxidant and anti-inflammatory activities. The ethanol extract of the aerial parts contained flavonoid compounds, while the root extracts and dichloromethane extract did not. The extracts exhibited strong antioxidant activity and efficiently inhibited the production of pro-inflammatory cytokines. Particularly, the dichloromethane extract of the root showed the strongest activity and contained fatty acids and triterpenoids.
Article
Green & Sustainable Science & Technology
Fatemeh Soltanzadeh, Ali Edalat-Behbahani, Kasra Hosseinmostofi, Ibrahim Fatih Cengiz, Joaquim Miguel Oliveira, Rui L. Reis
Summary: This study investigated the bond durability characteristics of basalt-fiber-reinforced polymer (BFRP) rebars in fiber-reinforced self-compacting concrete (FRSCC) structures. The influence of environmental conditions, reinforcement type, and loading type on the bond strength of the specimens was explored. The results were used to estimate the bond strength retention between BFRP and FRSCC after 50 years of exposure to seawater.
