Article
Medicine, Research & Experimental
Moriah J. Hovde, Danielle E. Bolland, Aryna Armand, Emily Pitsch, Clare Bakker, Amanda J. Kooiker, Joseph J. Provost, Roxanne A. Vaughan, Mark A. Wallert, James D. Foster
Summary: This study demonstrates for the first time that NHE1 is palmitoylated in both cells and rat tissue, and that its palmitoylation status is associated with the regulation of intracellular pH, stress fiber formation, and cell migration. Furthermore, it is shown that LPA stimulates NHE1 palmitoylation, and pretreatment with 2BP inhibits LPA-induced increase in intracellular pH. These findings highlight the significance of palmitoylation in the regulation of NHE1 and other control/signaling proteins.
Review
Biochemistry & Molecular Biology
Alice Main, William Fuller
Summary: This review discusses the role of lipid post-translational modification S-palmitoylation in diseases and the advancements in experimental tools in the field. Understanding how palmitoylation controls protein localization and function may lead to its modulation as a therapeutic strategy in the future.
Article
Ecology
Arkadeep Mukherjee, Bharti Thakur, Ajay Kumar Pandey, Roland Marmeisse, Laurence Fraissinet-Tachet, M. Sudhakara Reddy
Summary: The study focuses on investigating the functional genes of eukaryotic microbiota in metal polluted environments using metatranscriptomics. By constructing size fractionated cDNA libraries and screening for Cadmium tolerant genes, a potential metal tolerant gene PLCg39 was identified, which could be utilized in bioremediation, biosensing, and other biotechnological fields.
Article
Neurosciences
Fanny L. Lemarie, Nicholas S. Caron, Shaun S. Sanders, Mandi E. Schmidt, Yen T. N. Nguyen, Seunghyun Ko, Xiaohong Xu, Mahmoud A. Pouladi, Dale D. O. Martin, Michael R. Hayden
Summary: Research has shown that palmitoylation of mHTT and HIP14/HIP14L substrates is decreased early in Huntington's disease models, further decreasing with aging. Palmitoylation of mHTT can be normalized using an APT inhibitor, reducing mHTT aggregation and cytotoxicity.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Medicine, Research & Experimental
Dongze Li, Li Zhang, Yanqiu He, Tingting Zhou, Xi Cheng, Wei Huang, Yong Xu
Summary: Diabetes is a prevalent global epidemic disease that is influenced by genetic, environmental, and socioeconomic factors. Histone post-translational modifications, specifically methylation and acetylation, play significant roles in the pathophysiological changes associated with diabetes. Recent advancements in mass spectrometry have identified novel histone acylation modifications, which are yet to be thoroughly investigated for their connection to diabetes.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Microbiology
Juan F. Martin, Paloma Liras, Sergio Sanchez
Summary: Various post-translational modifications are found in bacteria, such as phosphorylation, acetylation, or phosphopantetheinylation, which play essential roles in bacterial metabolism and secondary metabolite biosynthesis. These modifications can control the activity of key enzymes and transcriptional factors involved in biosynthesis, affecting the expression of genes and production of bioactive metabolites.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Cell Biology
Muhammad U. Anwar, F. Gisou van der Goot
Summary: S-acylation is an important posttranslational modification that regulates cellular processes. This reversible lipid modification affects cellular pathways and physiological processes, and the enzymes and proteins involved in S-acylation are still being discovered and studied.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Plant Sciences
Jincheng Li, Manqi Zhang, Lijuan Zhou
Summary: Protein S-acylation, also known as palmitoylation, is a vital lipid post-translational modification in eukaryotes, playing critical roles in plant development and responses to various stresses. The dynamic and reversible nature of S-acylation allows membrane-associated proteins to undergo cycling and trafficking, acting as a switch in signaling transductions. While numerous S-acylated proteins have been identified in plants, the understanding of the regulatory mechanisms controlling this process remains incomplete.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Simon Legood, Ivo G. Boneca, Nienke Buddelmeijer
Summary: Lipoproteins play crucial roles in bacterial cells, with their modification process involving complex enzymes and phospholipids, recently elucidated by X-ray crystallography. Given the essential nature of lipoprotein modification in proteobacteria, it serves as a potential target for the development of novel antibiotics.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Robert W. B. Brown, Aabha Sharma, Miguel Rey Villanueva, Xiaomo Li, Ouma Onguka, Leeor Zilbermintz, Helen Nguyen, Ben A. Falk, Cheryl L. Olson, Joann M. Taylor, Conrad L. Epting, Rahul S. Kathayat, Neri Amara, Bryan C. Dickinson, Matthew Bogyo, David M. Engman
Summary: Dynamic post-translational modifications in eukaryotic cells allow rapid and specific regulation of protein functions. The S-acylation process involves reversible lipid modification of proteins, while depalmitoylation is necessary for acylation homeostasis. The identification of a homologue of human APT1 in Trypanosoma brucei, named TbAPT-L, suggests a lipase function rather than depalmitoylase activity, with modulation of TbAPT-L showing no effect on parasite growth or cellular depalmitoylation activity in vitro.
Review
Biochemistry & Molecular Biology
Palmiro Poltronieri, Masanao Miwa, Mitsuko Masutani
Summary: ADP-ribosylation plays a crucial role in post-translational modifications of proteins, involving functions such as DNA repair, transcription, and cell signaling. Enzymes involved in monoADP-ribosylation/polyADP-ribosylation (MAR/PAR) cycling also play important roles in cancers, with potential applications in non-oncological diseases. The field is still actively uncovering novel enzymes at a rapid pace.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xing Gao, Chien-Wen Kuo, Alice Main, Elaine Brown, Francisco J. Rios, Livia De Lucca Camargo, Sheon Mary, Krzysztof Wypijewski, Caglar Gok, Rhian M. Touyz, William Fuller
Summary: This study identifies a new mechanism for regulating TrpM7 through palmitoylation. Palmitoylation controls the exit of TrpM7 from the endoplasmic reticulum and its distribution between the cell surface and intracellular vesicles. Palmitoylation is closely related to the ion channel activity and trafficking of TrpM7.
Article
Biochemistry & Molecular Biology
Yue Xiao, Mingyu Yin, Yiyi Wang, Rongyi Shi, Shuyu Mao, Yuejin Hua, Hong Xu
Summary: This article studied the biological role of acyl modification of FEN1 in HeLa cells. The absence of acylation of FEN1 leads to blocked cell cycle process and reduced efficiency in DNA repair, affecting its interaction with repair and replication related proteins and its role in DNA damage repair.
PROTEIN AND PEPTIDE LETTERS
(2023)
Review
Biochemistry & Molecular Biology
Niall J. Fraser, Jacqueline Howie, Krzysztof J. Wypijewski, William Fuller
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Editorial Material
Cardiac & Cardiovascular Systems
Alice Main, Olivia Robertson-Gray, William Fuller
CARDIOVASCULAR RESEARCH
(2021)
Review
Cell Biology
Alice Main, William Fuller, George S. Baillie
CELLULAR SIGNALLING
(2020)
Review
Cell Biology
Caglar Goek, William Fuller
CELLULAR SIGNALLING
(2020)
Article
Cardiac & Cardiovascular Systems
Andrii Boguslavskyi, Sergiy Tokar, Oleksandra Prysyazhna, Olena Rudyk, David Sanchez-Tatay, Hamish A. L. Lemmey, Kim A. Dora, Christopher J. Garland, Helen R. Warren, Alexander Doney, Colin N. A. Palmer, Mark J. Caulfield, Julia Vlachaki Walker, Jacqueline Howie, William Fuller, Michael J. Shattock
Summary: Studies have shown the significance of phospholemman phosphorylation in regulating vascular tone and blood pressure, suggesting it as a potential novel mechanism and therapeutic target for aging-induced essential hypertension in humans.
Review
Biochemistry & Molecular Biology
Alice Main, William Fuller
Summary: This review discusses the role of lipid post-translational modification S-palmitoylation in diseases and the advancements in experimental tools in the field. Understanding how palmitoylation controls protein localization and function may lead to its modulation as a therapeutic strategy in the future.
Letter
Cardiac & Cardiovascular Systems
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R. Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J. Shattock
CARDIOVASCULAR RESEARCH
(2021)
Article
Cell Biology
Caglar Gok, Alice Main, Xing Gao, Zsombor Kerekes, Fiona Plain, Chien-Wen Kuo, Alan D. Robertson, Niall J. Fraser, William Fuller
Summary: Our study reveals that the identity of NCX1 palmitoylation machinery plays a key role in spatial organization within the cell, the amphipathic alpha-helix of NCX1 directly interacts with zDHHC-PATs, NCX1 is still palmitoylated when arrested in Golgi or ER, indicating it is a substrate for multiple zDHHC-PATs.
Article
Biochemistry & Molecular Biology
M. Kerr, K. M. J. H. Dennis, C. A. Carr, W. Fuller, G. Berridge, S. Rohling, C. L. Aitken, C. Lopez, R. Fischer, J. J. Miller, K. Clarke, D. J. Tyler, L. C. Heather
Summary: The bioactive lipid intermediate palmitoyl CoA (PCoA) inhibits mitochondrial ADP/ATP transport, protecting mitochondrial energetics during ischemia. However, this protective effect is blunted in diabetes, contributing to impaired energy metabolism during myocardial ischemia in the diabetic heart.
Article
Microbiology
Magali Van den Kerkhof, Philippe Leprohon, Dorien Mabille, Sarah Hendrickx, Lindsay B. Tulloch, Richard J. Wall, Susan Wyllie, Eric Chatelain, Charles E. Mowbray, Stephanie Braillard, Marc Ouellette, Louis Maes, Guy Caljon
Summary: Oxaboroles, a novel class of antileishmanial drugs, have not shown readily emergent resistance and hold promise for future treatment. The combination of a genome-wide cosmid library and next-generation sequencing can identify resistance determinants and potential targets.
Article
Cell Biology
Caglar Gok, Alan D. Robertson, William Fuller
Summary: In this study, we found that insulin regulates the activity of NCX1 by inducing its palmitoylation, thereby modulating Ca2+ handling in cardiac cells. By activating fatty acid and fatty acyl CoA synthesis, insulin promotes palmitoylation of the zDHHC5 active site, leading to enhanced NCX1 palmitoylation.
Article
Biochemistry & Molecular Biology
Juan F. Cervilla-Martinez, Juan J. Rodriguez-Gotor, Krzysztof J. Wypijewski, Angela Fontan-Lozano, Tao Wang, Enrique Santamaria, William Fuller, Rebeca Mejias
Summary: Through data-mining of palmitome in the cerebral cortex of PD patients and controls, the study identified 150 proteins with altered palmitoylation, which are predicted to impact cytoskeleton, mitochondrial and fibrinogen functions, as well as cell survival. The research suggests that protein palmitoylation may play a role in the pathophysiology of PD, and comprehensive palmitoyl-proteomics is a powerful approach for exploring novel cellular pathways involved in this neurodegenerative disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
William Fuller
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2023)
Article
Cardiac & Cardiovascular Systems
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R. Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J. Shattock
Summary: Research indicates that cardiac NHE1 activity is not inhibited by EMPA (or other SGLT2i's) and EMPA has no effect on [Na+] over a wide range of concentrations, including the therapeutic dose.
CARDIOVASCULAR RESEARCH
(2021)