期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 204, 期 -, 页码 45-50出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2017.11.010
关键词
Zebrafish embryo; Vitellogenin 1; Estrogenic effect; Endocrine disrupting chemicals
资金
- National Natural Science Foundation of China [21477049]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14030501]
- State Key Laboratory of Freshwater Ecology and Biotechnology [2014FB07]
By measuring the vitellogenin 1 (vtgl) expression through quantitative PCR and in situ hybridization, we used the zebrafish embryo as an in vivo model to access the estrogenic or anti-estrogenic effects of several endocrine disrupting chemicals (EDCs), such as natural estrogen 17 beta-estradiol (E2), estriol (E3), synthetic hormones including diethylstilbestrol (DES), 4-octyl phenol (OP), bisphenol A (BPA), tamoxifen (TMX) and 3-(2,3-dibromopropyl) isocyanurate (TBC). According to our data, the estrogenic effect Of the tested chemicals was ranked as: DES > E-2 > E-3 > OP > BPA, which is consistent with various in vivo and in vitro models. Therefore, the measurement of vtgl gene expression in zebrafish embryos would be a valuable method for screening EDCs including both environmental estrogens and anti-estrogens.
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