期刊
CLINICAL NUTRITION
卷 37, 期 2, 页码 443-451出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.clnu.2017.01.011
关键词
Ageing; Influenza; Probiotic; Lymphocyte; Vaccination
资金
- Biotechnology and Biological Sciences Research Council Diet and Health Research Industry Club (BBSRC-DRINC) [BB/H00470X/1]
- BBSRC [BB/H00470X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [974616, BB/H00470X/1] Funding Source: researchfish
Background & aims: Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum by. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects. Methods: In a double-blind, randomized controlled trial, 58 young (18-35 y) and 54 older (60-85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks. Results: B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA(+) memory, IgG(+) memory and total IgG(+)B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositiviry was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine. Conclusions: Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. (C) 2017 The Author(s). Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据