4.7 Article

Antioxidant and cryoprotective effects of Amur sturgeon skin gelatin hydrolysate in unwashed fish mince

期刊

FOOD CHEMISTRY
卷 181, 期 -, 页码 295-303

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.02.095

关键词

Amur sturgeon; Gelatin hydrolysate; Antioxidant activity; Cryoprotective effect; Unwashed fish mince

资金

  1. National Natural Science Foundation of China [31471584]
  2. project for National Key Technology R&D Program for the 12th Five-Year Plan [2012BAD37B06/7]

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Antioxidant and cryoprotective effects of Amur sturgeon skin gelatin hydrolysates prepared using different commercial proteases in unwashed fish mince were investigated. Gelatin hydrolysates prepared using either Alcalase or Flavourzyme, were effective in preventing lipid oxidation as evidenced by the lower thiobarbituric acid-reactive substances formation. Gelatin hydrolysates were able to retard protein oxidation as indicated by the retarded protein carbonyl formation and lower loss in sulfhydryl content. In the presence of gelatin hydrolysates, unwashed mince had higher transition temperature of myosin and higher enthalpy of myosin and actin as determined by differential scanning calorimetry. Based on low field proton nuclear magnetic resonance analysis, gelatin hydrolysates prevented the displacement of water molecules between the different compartments, thus stabilizing the water associated with myofibrils in unwashed mince induced by repeated freeze-thawing. Oligopeptides in gelatin hydrolysates more likely contributed to the cryoprotective effect. Thus, gelatin hydrolysate could act as both antioxidant and cryoprotectant in unwashed fish mince. (C) 2015 Elsevier Ltd. All rights reserved.

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