Article
Cardiac & Cardiovascular Systems
Hideaki Inazumi, Koichiro Kuwahara, Yasuaki Nakagawa, Yoshihiro Kuwabara, Takuro Numaga-Tomita, Toshihide Kashihara, Tsutomu Nakada, Nagomi Kurebayashi, Miku Oya, Miki Nonaka, Masami Sugihara, Hideyuki Kinoshita, Kenji Moriuchi, Hiromu Yanagisawa, Toshio Nishikimi, Hirohiko Motoki, Mitsuhiko Yamada, Sachio Morimoto, Kinya Otsu, Richard M. Mortensen, Kazuwa Nakao, Takeshi Kimura
Summary: NRSF is a transcriptional repressor that suppresses the reactivation of fetal cardiac gene program and maintains cardiac integrity. This study reveals that NRSF regulates ventricular function by controlling the expression of G alpha(o) gene, and excessive expression of G alpha(o) leads to cardiac dysfunction. Additionally, G alpha(o) plays a critical role in cardiac Ca2+ homeostasis and systolic function.
CIRCULATION RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Ahmed Rakib, Taslima Akter Eva, Saad Ahmed Sami, Saikat Mitra, Iqbal Hossain Nafiz, Ayan Das, Abu Montakim Tareq, Firzan Nainu, Kuldeep Dhama, Talha Bin Emran, Jesus Simal-Gandara
Summary: Heart failure is a complex clinical syndrome that is often the result of long-standing hypertension. Recent advances in hypertension treatment include novel pathways such as the potential role of beta-arrestin in regulating hypertension. However, there are still controversies surrounding the roles of the two beta-arrestin isoforms in the treatment of HF.
Article
Biochemical Research Methods
Michael J. Swierczynski, Yuxuan Ding, Zachary T. Ball
Summary: Boronic acids and boronate esters are widely used in chemical biology for the preparation of complex bioconjugates. This paper presents the expansion of bis-boronic acid reagents for tandem covalent and dynamic bioconjugation. The study provides new insights into the kinetic stability of the bioconjugates.
BIOCONJUGATE CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Agnieszka Polit, Pawel Mystek, Ewa Blasiak
Summary: This passage discusses how individual cell functions in multicellular organisms rely on signal transduction through GPCRs and G proteins, with a focus on the protein-lipid interactions and G proteins' attachment to cell membranes. While the interaction mode between the cell membrane and G proteins is well known, the molecular-level recognition mechanisms and the interrelation of individual G protein-membrane attachment signals in controlling membrane targeting remain unclear.
Article
Agriculture, Dairy & Animal Science
Jinhee H. Hwang, Michael E. Spurlock, John C. Kube, Xiang Z. Li, Stephen B. Smith
Summary: The study revealed that lubabegron fumarate is highly selective for the beta(3)-adrenergic receptor subtype and has minimal agonistic activity at the beta(2)-adrenergic receptor. Differences in mRNA expression levels of beta(1)-, beta(2)-, and beta(3)-AR were observed between subcutaneous and intramuscular adipose tissues. The response to beta-adrenergic ligands, especially agonists for the beta(1)- and beta(3)-receptor subtypes, was found to be lower in intramuscular adipose tissue compared to subcutaneous adipose tissue.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Hani N. Sabbah, Kefei Zhang, Ramesh C. Gupta, Jiang Xu, Vinita Singh-Gupta, Michael Ma, Kathe Stauber, Nathalie Nguyen, John Adams
Summary: Intravenous infusions of the beta(3)-AR antagonist APD418 significantly improved cardiac function in dogs with systolic heart failure, as evidenced by increased LVEF and Ei/Ai. These findings suggest the potential of APD418 for the in-hospital treatment of patients with an acute exacerbation of chronic heart failure.
JOURNAL OF CARDIAC FAILURE
(2021)
Review
Biochemistry & Molecular Biology
Krzysztof Jozwiak, Anita Plazinska
Summary: Studies on different receptors belonging to class A of GPCRs reveal specific molecular mechanisms behind ligand directed signaling, including the role of important residues, the impact of ligand structural features on signaling, and the key interactions between ligands and receptors.
Review
Biochemistry & Molecular Biology
Ritesh Urade, Yan-Hui Chiu, Chien-Chih Chiu, Chang-Yi Wu
Summary: This article discusses the role of Ras superfamily proteins in the development of the vascular system in zebrafish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaoyu Tian, Ziwei Huang, Yuanping Wang, Xiaoxiao Qi, Dawei Wang, Zhongqiu Liu, Yuanyuan Cheng
Summary: This study revealed that Xinbao Pill (XBP) improved cardiac function against chronic heart failure (CHF) by suppressing the ubiquitination of ll1-AR and ll2-AR mediated by USP18 and MDM2/ll-arrestin2/Nedd4.
Review
Biochemistry & Molecular Biology
Igor Kasheverov, Denis Kudryavtsev, Irina Shelukhina, Georgy Nikolaev, Yuri Utkin, Victor Tsetlin
Summary: The review highlights the importance of marine compounds in studying the structure and mechanism of nAChRs, as well as in drug discovery. Different classes of ligands, ranging from low molecular weight compounds to peptides and proteins, have been used to characterize and differentiate nAChRs subtypes. The use of sophisticated techniques, such as computer modeling and X-ray analysis, has provided valuable insights into the orthosteric and allosteric binding sites of nAChRs.
Article
Biochemical Research Methods
Ali Isbilir, Robert Serfling, Jan Moeller, Romy Thomas, Chiara De Faveri, Ulrike Zabel, Marco Scarselli, Annette G. Beck-Sickinger, Andreas Bock, Irene Coin, Martin J. Lohse, Paolo Annibale
Summary: Fluorescence imaging methods are becoming an effective tool for quantifying membrane protein oligomerization, with a detailed protocol provided here for determining the number and oligomerization state of fluorescently labeled GPCRs. The protocol includes steps for flexible labeling strategies, image acquisition on a confocal microscope, and data analysis, allowing for application to diverse classes of membrane proteins of interest.
Article
Multidisciplinary Sciences
Zsuzsanna Z. A. Kovacs, Gergo Szucs, Marah Freiwan, Monika G. Kovacs, Fanni M. Marvanykovi, Hoa Dinh, Andrea Siska, Katalin Farkas, Ferenc Kovacs, Andras Kriston, Peter Horvath, Bence Kovari, Balint Gabor Cserni, Gabor Cserni, Imre Foldesi, Tamas Csont, Marta Sarkozy
Summary: In this study, the antiremodeling effects of losartan and mirabegron in uremic cardiomyopathy were compared. Losartan significantly improved heart function and structure, while mirabegron showed some improvement in diastolic dysfunction and fibrosis, but did not significantly affect eNOS expression.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Zhansong Lin, Arman A. Bashirova, Mathias Viard, Lee Garner, Max Quastel, Maya Beiersdorfer, Wojciech K. Kasprzak, Marjan Akdag, Yuko Yuki, Pedro Ojeda, Sudipto Das, Thorkell Andresson, Vivek Naranbhai, Amir Horowitz, Andrew J. McMichael, Angelique Hoelzemer, Geraldine M. Gillespie, Wilfredo F. Garcia-Beltran, Mary Carrington
Summary: Human leukocyte antigen (HLA)-E binds epitopes derived from HLA-A, HLA-B, HLA-C and HLA-G signal peptides (SPs) and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors. Carrington and colleagues provide comprehensive analysis of classical HLA class I SP variants and show that these can determine CD94/NKG2-HLA-E engagement. The systematic, quantitative approach described herein will facilitate development of prediction algorithms for accurately measuring the impact of CD94/NKG2-HLA-E interactions in disease resistance/susceptibility.
Article
Multidisciplinary Sciences
Wen Cui, Elisabeth Braun, Wei Wang, Jinhong Tang, Yanyan Zheng, Benjamin Slater, Na Li, Cheng Chen, Qingxiang Liu, Bin Wang, Xiu Li, Yinkai Duan, Yunjie Xiao, Ruijiao Ti, Dominik Hotter, Xiaoyun Ji, Lei Zhang, Jun Cui, Yong Xiong, Daniel Sauter, Zefang Wang, Frank Kirchhoff, Haitao Yang
Summary: This study presents crystal structures of Guanylate-binding proteins and reveals a GTP-induced dimerization mode that is likely conserved among all GBPs, providing insights into the molecular determinants of their antiviral function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Kiall F. Suazo, Angela Jeong, Mina Ahmadi, Caroline Brown, Wenhui Qu, Ling Li, Mark D. Distefano
Summary: Protein prenylation is crucial for signal transduction processes, and using the probe C15AlkOPP, this study explored metabolic labeling and identification of prenylated proteins in various cell lines. The research identified common prenylated proteins in three brain-related cell lines and also unique prenylated proteins in each type. Additionally, inhibition of farnesylation in primary astrocytes showed different responses of farnesylated proteins to an FTI, suggesting the potential utility of this chemical proteomic approach in studying prenylated proteins in different diseases.
SCIENTIFIC REPORTS
(2021)
