期刊
CHEMPHYSCHEM
卷 19, 期 18, 页码 2299-2304出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201800396
关键词
higher-order structure of DNA; branched polyamine; archaea; high temperature; nano-loop
资金
- JSPS KAKENHI [15H02121, 25103012]
- Grants-in-Aid for Scientific Research [15H02121] Funding Source: KAKEN
A pentavalent branched-chain polyamine, N-4-bis(aminopropyl)spermidine 3(3)(3)4, is a unique polycation found in the hyperthermophilic archaeon Thermococcus kodakarensis, which grows at temperatures between 60 and 100 degrees C. We studied the effects of this branched-chain polyamine on DNA structure at different temperatures up to 80 degrees C. Atomic force microscopic observation revealed that 3(3)(3)4 induces a mesh-like structure on a large DNA (166kbp) at 24 degrees C. With an increase in temperature, DNA molecules tend to unwind, and multiple nano-loops with a diameter of 10-50nm are generated along the DNA strand at 80 degrees C. These results were compared to those obtained with linear-chain polyamines, homocaldopentamine 3334 and spermidine, the former of which is a structural isomer of 3(3)(3)4. These specific effects are expected to neatly concern with its role on high-temperature preference in hyperthermophiles.
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