Chlorella vulgaris mixotrophic growth enhanced biomass productivity and reduced toxicity from agro-industrial by-products

Algal wastewater remediation has become attractive for a couple of years now, however the effectiveness of genetic toxicity reducing of some by-products through microalgae are still not well reported. This study aimed to evaluate the growth, nutrients and toxicity removal of Chlorella vulgaris cultivated under autotrophic and mixotrophic conditions in three agro-industrial by-products. Mixotrophic culture using corn steep liquor showed higher cell concentration, specific growth rate, maximum cell productivity and biomass protein content when compared to cheese whey and vinasse. Nutrient removal results showed that C vulgaris was able to completely remove corn steep liquor nutrients, while in cheese whey and vinasse culture this removal was not as efficient, observing remaining COD. This work evaluated for the first time the corn steep liquor and cheese whey genetic toxicity through Allium cepa seeds assay. These results demonstrate that corn steep liquor toxicity was totally eliminated by C vulgaris cultivation, and cheese whey and vinasse toxicity were minimized. This study proves that the mixotrophic cultivation of C. vulgaris can increase cellular productivity, as well as it is a suitable and economic alternative to remove the toxicity from agroindustrial by-products. (C) 2018 Elsevier Ltd. All rights reserved.