期刊
CHEMICAL ENGINEERING SCIENCE
卷 191, 期 -, 页码 232-244出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ces.2018.06.066
关键词
Protein crystallization; Droplet-based microfluidics; Phase diagram; Nucleation
资金
- European Research Council [677169-MicroParticleControl]
- FCT [SFRH/BPD/96132/2013, POCI-01-0145-FEDER-006939, UID/EQU/00511/2013]
- European Regional Development Fund (ERDF), through COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI)
- FCT - Fundacao para a Ciencia e a Tecnologia
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/96132/2013, UID/EQU/00511/2013] Funding Source: FCT
This work reports a cheap and easy-to-use droplet-based microfluidic platform for the study of protein crystallization, offering the possibility to characterize the protein phase behaviour, and the effect of volumetric and interfacial phenomena on the crystallization mechanism. We conducted a parametric study supported by comparison with literature data, to quantify the influence of the droplet volume on the thermodynamic (solubility data) and kinetic (metastability data) parameters, using lysozyme as a model protein. Experiments were performed in a tubular microreactor at low Capillary numbers (4.1 x 10(-5)-2.3 x 10(-4)), resulting in a broad range of droplet sizes. The droplet formation in a flowfocusing geometry was also numerically studied using CFD and a correlation for the droplet size was developed. Subsequently, the lysozyme phase behaviour and the possible mechanisms associated with the nucleation process were evaluated. While crystallization in small volume droplets is usually characterized by a low nucleation probability and correspondingly low number of crystals, we did not observe this in our experiments. A potential explanation for this is the complex and stochastic mechanism of nucleation, including the competition between monomers and oligomers in solution. (C) 2018 The Authors. Published by Elsevier Ltd.
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