Article
Food Science & Technology
Shixiang Wu, Xingping Zhang, Wang Chen, Guohao Zhang, Qian Zhang, Hualin Yang, Yu Zhou
Summary: Inspired by the strong binding capacity of pyrophosphate (ppi) and copper ion (Cu2+), a ratiometric fluorescence immunoassay triggered by alkaline phosphatase (ALP) was developed for the detection of zearalenone (ZEN). This method exhibited high accuracy and reliability, with a limit of detection (LOD) of 0.014 ng/mL and a linear detection range of 3.51-17.78 ng/mL. It provides a new approach for efficient and accurate detection of ZEN.
Article
Chemistry, Multidisciplinary
Yangyang Chang, Qian Zhang, Wei Xue, Yanfang Wu, Yi Liu, Meng Liu
Summary: We developed a paper-based analytical device that utilizes 3D DNA encapsulating horseradish peroxidase (HRP) for detecting alkaline phosphatase (ALP) visually. This device allows for on-paper sample pre-treatment, target recognition, and signal readout, enabling simple and rapid determination of ALP in clinical samples without the need for additional blood sample pre-treatment (within 23 minutes).
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Cuiling Shang, Yansong Li, Qi Zhang, Song Tang, Xiaoqian Tang, Honglin Ren, Pan Hu, Shiying Lu, Peiwu Li, Yu Zhou
Summary: A dual-signal immunoassay utilizing alkaline phosphatase was developed to detect Zearalenone (ZEN) in cornmeal. The method combines colorimetric and electrochemical detection, generating Prussian blue nanoparticles and multicolor changes. The results demonstrate a high sensitivity for ZEN detection in corn samples through UV-vis spectrometry and differential pulse voltammetry.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Xiaolong Zheng, Yanan Zhao, Yan Zhang, Yuanhua Zhu, Junxiang Zhang, Die Xu, Hualin Yang, Yu Zhou
Summary: In this study, a feasible, sensitive, and selective fluorescence method was developed for the detection of OTA. The method utilized alkaline phosphatase-triggered gold nanoclusters as a fluorescent probe, and showed good linearity and low detection limit.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Chemistry, Analytical
Ting Zhu, Jinyang Chen, Qingli Chai, Shasha Zeng, Yucheng Liu
Summary: This study presents a novel and stable fluorescent sensor based on the wavelength shift of copper nanoclusters (CuNCs) as signal readout. By introducing manganese ion (Mn2+), the fluorescence wavelength of CuNCs could be precisely tuned, and it is demonstrated that wavelength readout is more stable than intensity readout. A stable sensor for sensitive alkaline phosphatase (ALP) detection is constructed based on this principle, and consistent results with clinical test data are obtained in real serum samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Liping Gao, Yong Li, Zhen-Zhong Huang, Hongliang Tan
Summary: The study presents a stimuli-responsive alginate hydrogel for visual detection of alkaline phosphatase by modulating the gel-sol transition process through AA-triggered dissolution. This simple visual method shows low detection limit, excellent selectivity, and advantages of easy fabrication and cost-effectiveness compared to conventional colorimetric assays. The research provides new insights for potential applications in chemical sensing and biomedical fields.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Eslam Hafez, Byeong-Seok Moon, Samy M. Shaban, Do-Gi Pyun, Dong-Hwan Kim
Summary: A colorimetric biosensor for ALP assay has been developed based on ALP affinity to dephosphorylate glucose phosphate into glucose, which provides a multicolor response with a high sensitivity and selectivity for point-of-care diagnosis.
Article
Engineering, Biomedical
Chengfan Wu, Chenchen Wang, Tong Zhang, Ge Gao, Mengxing Wei, Yinglu Chen, Xiaoyan Li, Fuqiang Wang, Gaolin Liang
Summary: A novel compound, Py-Yp-Lyso, was designed and tested in this study for its lysosome-targeting cytotoxicity on cancer cells, which is achieved through ALP-guided self-assembly, inducing LMP and leading to cancer cell death in a selective manner.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biophysics
Liu Liu, Hui Jiang, Xuemei Wang
Summary: A novel fluorescent hydrogel material is developed, with good optical properties and mechanical structure, capable of releasing fluorescent gold nanoclusters induced by free phosphate ions. This material shows high sensitivity to alkaline phosphatase and can accurately detect enzyme levels in human serum samples. Furthermore, the hydrogel also exhibits good peroxidase-like activity and is easily recyclable.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Yujie Sun, Lei Wen, Huili Ma, Wenlin Ma, Zhenqian Fu, Yinhui Li, Chengwu Zhang, Lin Li, Jinhua Liu
Summary: In this study, a trienzyme cascade-triggered fluorescent immunosensor platform was constructed by integrating alkaline phosphatase (ALP), tyrosinase (TYR), and horseradish peroxidase (HRP). The platform utilized a rapid in situ fluorogenic reaction to produce a yellow fluorescent compound. The developed immunosensor showed significant enhancements in fluorescence and successfully detected a cardiac biomarker in clinical samples.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Jasvir Kaur, Prabhat K. Singh
Summary: The study introduced a fluorescence turn-on probing system for monitoring ALP activity by utilizing the unique phenomenon of aggregation induced emission (AIE), demonstrating rapid, easy, sensitive and highly selective detection. The sensor showed remarkable specificity for ALP and successfully displayed potential application in human serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Multidisciplinary
Meng Li, Bhaskar Gurram, Shan Lei, Nicholas T. Blum, Peng Huang, Jing Lin
Summary: Phosphatase is crucial in many biological functions, with alkaline phosphatase (ALP) serving as a key prognostic indicator for various diseases, though current detection methods are limited to in vitro colorimetric techniques. Fluorescence imaging provides a reliable way to monitor real-time changes in ALP levels, with research focusing mainly on ALP imaging probes grouped by their luminescence mechanisms. Challenges and future prospects for phosphatase probes were also discussed.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Guobin Mao, Chunmin Qiu, Xing Luo, Yingqi Liang, Liqing Zhao, Weiren Huang, Junbiao Dai, Yingxin Ma
Summary: A cyan-emitting fluorescent Si dots for alkaline phosphatase (ALP) detection was prepared through the synergistic quenching effect of inner filter effect (IFE) and photo-induced electron transfer (PET). Si dots displayed high quantum yield and good physiochemical properties. The turn-off mode detection based on the synergistic quenching effect showed high sensitivity and great anti-interference ability in complex environments, enabling accurate detection of ALP in human serum.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Haifeng Sha, Bing Yan
Summary: A luminescent probe was synthesized by capturing terbium ions with a self-assembled structure for the detection of alkaline phosphatase activity. The probe showed ultra-sensitivity and high selectivity, and demonstrated good practical application prospects in serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Xiaoqian Huang, Xiangzhu Chen, Shijun Chen, Xueyan Zhang, Lin Wang, Shicong Hou, Xiaodong Ma
Summary: A novel ratiometric fluorescent probe has been developed for the detection of alkaline phosphatase (ALP) and fluorescence imaging in living cells, showing high accuracy and sensitivity. The probe exhibits a significant fluorescence color change from blue to green in response to ALP, with a detection limit as low as 0.16 U L-1. Additionally, the probe demonstrates excellent biocompatibility for application in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Xiang Shi, Ge Gao, Xiaoyang Liu, Lingling Xu, Yu Deng, Rui Wang, Gaolin Liang
Summary: Lipid droplet (LD) fluorescent imaging plays an important role in the detection of lipid-related diseases. However, currently available LD imaging fluorophores have limitations in terms of photostability and hydrophobicity. This study introduces a highly lipophilic compound, Cou-Flu, which exhibits excellent photostability and excimer-monomer transition property. Cou-Flu enables high contrast LD imaging and LD movement tracing in cells, and it is also suitable for in vivo LD detection with excellent sensitivity.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biophysics
Xingxing Ren, Menglin Tao, Xiaoming Liu, Lele Zhang, Mingsong Li, Zijuan Hai
Summary: In this study, we developed two activatable caspase-1-responsive fluorescence biosensors that can efficiently and rapidly monitor inflammasome activation, with the potential for clinical diagnosis of related inflammatory diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Zhen Zheng, Tiantian Xia, Tao Wang, Deying Jia, Ziye Wu, Jun Jiang, Gaolin Liang
Summary: By realizing the firefly bioluminescence cycle in vitro and in cells, researchers have identified the optimal chemical route to achieve the highest light intensity. This pioneering study provides the opportunity to design tunable, economical, biomimetic cold light devices in the near future.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Biomedical
Lele Zhang, Minghui Wang, Fangzheng Wu, Li Liu, Xingxing Ren, Zijuan Hai
Summary: In this study, a probe named Probe-1 was developed to enhance photoacoustic imaging (PAI) and photothermal therapy (PTT) of liver tumors through the self-assembly of Hemicyanine (HCy) nanoparticles guided by glycyrrhetinic acid (GA) targeting and intracellular alkaline phosphatase (ALP) activity. Probe-1 showed significantly higher PAI signal and lower inhibitory concentrations in HepG2 cells compared to Probe-2 without self-assembly ability. In addition, Probe-1 exhibited extended retention time and higher PAI signal in HepG2 tumors, leading to a larger increase in temperature and decreased tumor volume compared to Probe-2. This smart self-assembly strategy can potentially be applied for PAI and PTT of other tumors.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Analytical
Jiajun Liu, Fangzheng Wu, Minghui Wang, Menglin Tao, Zhengjie Liu, Zijuan Hai
Summary: A tumor-targeted fluorescent/photoacoustic probe was developed to monitor the activity of Caspase-3 in vivo. The probe could enter and accumulate in tumor cells, resulting in increased fluorescence and photoacoustic signals. This study provides a new method for clinical imaging of tumor apoptosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xianbao Sun, Tiantian Xia, Lingling Xu, Wenjun Zhan, Gaolin Liang
Summary: Featuring molecule-level data encryption, molecular keypad locks offer attractive advantages in information security. Previous multiple-input locks using fluorescence as the output have encountered challenges due to inefficient prequenching or the synthetic complexity of fluorophore-containing processor molecules. In this study, we propose a simple and label-free three-input molecular keypad lock, capable of generating fluorescent readout without background interference. The correct password leads to the generation of a fluorescent product, while incorrect inputs fail to open the lock. This device shows potential for further development into an advanced and secure keypad lock.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yu Deng, Xiang Shi, Xinyi Hu, Lingling Xu, Xiaoyang Liu, Ge Gao, Rui Wang, Gaolin Liang
Summary: Peroxynitrite (ONOO-) is an important reactive oxygen/nitrogen species (ROS/RNS) that plays a vital role in physiological activities. Excessive ONOO- is associated with various diseases, including inflammation, arthritis, inflammatory bowel disease, cancer, and neurodegenerative diseases. In this study, a chemiluminescent probe, B-PD, was designed and synthesized to detect endogenous ONOO-. The probe showed a quick response and a detection limit of 201 nM. In vivo imaging results demonstrated that B-PD could effectively locate early stage inflammation tissue with a high imaging contrast of up to 6.2. These findings suggest that B-PD holds great promise for highly sensitive diagnosis of inflammation-related diseases in the future.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mengxing Wei, Lulu Wang, Yanfang Wang, Tong Zhang, Chenchen Wang, Chengfan Wu, Changlin Tian, Gaolin Liang, Yue Yuan
Summary: Magnetic resonance imaging (MRI) is an excellent imaging technique with unlimited tissue penetration depth and superb spatiotemporal resolution. However, the use of intracellular self-assembled Gd-containing nanoparticles to enhance T-2-weighted MR contrast in vivo for precise tumor MRI is rarely reported. This study developed a CTSB-responsive small molecular contrast agent VC-Gd-CBT, which can self-assemble into large intracellular Gd-containing nanoparticles, and it has the potential to be used as a T-2 contrast agent for early-stage cancer diagnosis.
Article
Chemistry, Multidisciplinary
Guangqiong Xu, Qiao Weng, Lei Zhang, Yaye Song, Wenjun Zhan, Yuan Yin, Gaolin Liang, Fuqiang Wang
Summary: This study reports an AURKB-responsive hydrogel, Gel S/AZD, which enhances cervical tumor suppression by controlled release of AZD1152. Cell assays show that Gel S/AZD induces growth arrest and apoptosis of cervical cancer cells by downregulating the expression of AURKB downstream protein pH3. In vivo studies demonstrate that Gel S/AZD exhibits superior tumor suppressive effect in orthotropic cervical tumor models.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Xinyi Hu, Xianbao Sun, Xiaoyang Liu, Hai-Dong Xu, Liang Yang, Songqin Liu, Rui Wang, Gaolin Liang
Summary: This study introduces a new molecular probe for photoacoustic imaging, which shows promising results for early diagnosis of breast cancer. The experimental results demonstrate that the molecular probe enhances the activity of urokinase-type plasminogen activator and holds great potential for application in both in vitro and in vivo settings.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Hai-Dong Xu, Xiaotong Cheng, Xianbao Sun, Peiyao Chen, Wenjun Zhan, Xiaoyang Liu, Xinliang Wang, Bing Hu, Gaolin Liang
Summary: A self-assembling probe for apoptosis imaging was designed, which underwent a Caspase-3 cleavage and CBT-Cys click reaction to form Gd nanoparticles. This probe achieved enhanced MR imaging of apoptosis in vitro, in apoptotic cells, and in zebrafish.
Review
Chemistry, Analytical
Ying Xu, Qiaochu Jiang, Xianbao Sun, Gaolin Liang
Summary: Stimuli-triggered in-situ morphological transformation of peptide nanomaterials can enhance the on-site accumulation and retention of imaging agent cargos, enabling precise, sensitive, and prolonged disease imaging. This strategy allows for the co-delivery of contrast agents and drugs with smart turn-on ability, facilitating efficient disease theranostics. This review highlights the bioimaging applications of these smart morphological transformation-based peptide materials, and discusses the challenges in this field.
ANALYSIS & SENSING
(2023)
Review
Nanoscience & Nanotechnology
Xinyi Hu, Runqun Tang, Lin Bai, Songqin Liu, Gaolin Liang, Xianbao Sun
Summary: Derived from the D-luciferin regeneration pathway in firefly body, the CBT-Cys click reaction possesses unique advantages and has been used to fabricate stimuli-activatable imaging probes with self-assembling behaviors in physiological context. The in situ formed nanostructures enable precise and enhanced in vivo imaging of diseases, especially tumors.
Article
Chemistry, Multidisciplinary
Lingling Xu, Hang Gao, Wenjun Zhan, Yu Deng, Xiaoyang Liu, Qiaochu Jiang, Xianbao Sun, Jing-Juan Xu, Gaolin Liang
Summary: This study successfully achieves enhanced imaging of Alzheimer's disease by designing a near-infrared AIEgen with dual aggregation properties.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Yuan Mei, Ziyun Li, Kuanrong Rong, Zijuan Hai, Wenjian Tang, Qin-Hua Song
Summary: A dual functional BODIPY fluorescent probe has been developed for simultaneous detection of H2O2 and viscosity. Bioimaging results show that H2O2 accumulates and cytoplasmic viscosity increases during PA-induced pyroptosis.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
