Article
Agriculture, Dairy & Animal Science
Rui Zhang, Christiane Neuhoff, Qin Yang, Mehmet U. Cinar, Muhammad J. Uddin, Ernst Tholen, Karl Schellander, Dawit Tesfaye
Summary: This study found that sulforaphane (SFN) can enhance the proliferation of porcine satellite cells (PSCs), alter the gene expression levels of myogenic regulatory factors, inhibit histone deacetylase (HDAC) activity, and increase the expression of Smad family member 7 (SMAD7) in PSCs. Furthermore, SFN was found to increase the acetylation level of histone H4 in the SMAD7 promoter and decrease the expression of certain microRNAs that target SMAD7 in PSCs.
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yu Shi, Xudong Mao, Mingcheng Cai, Shenqiang Hu, Xiulan Lai, Shiyi Chen, Xianbo Jia, Jie Wang, Songjia Lai
Summary: miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells by targeting Mef2c.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Veterinary Sciences
Jie Song, Linlin Hao, Xiangfang Zeng, Rui Yang, Shiyan Qiao, Chunli Wang, Hao Yu, Siyao Wang, Yingying Jiao, Hongyao Jia, Songcai Liu, Ying Zhang
Summary: This study aimed to investigate the effects of novel miRNA Y-56 on porcine skeletal muscle satellite cells (PSCs) and found that Y-56 inhibited cell proliferation and cell cycle by targeting the IGF-1R-mediated AKT and ERK pathways.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xin Yang, Zhenhui Li, Zhijun Wang, Jiaao Yu, Manting Ma, Qinghua Nie
Summary: In this study, the researchers found differential expression of miR-27b-3p and Cbl-b genes in an induced atrophy model. They confirmed that miR-27b-3p can regulate the expression of Cbl-b gene. Cbl-b gene plays an important role in muscle atrophy, as its overexpression can upregulate the expression of marker genes related to muscle atrophy, while silencing Cbl-b expression can alleviate muscle atrophy.
Article
Genetics & Heredity
Yun Zhu, Peng Li, Xingang Dan, Xiaolong Kang, Yun Ma, Yuangang Shi
Summary: miR-377 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells through targeting FHL2 and attenuating the Wnt/beta-catenin signaling pathway.
Article
Agriculture, Dairy & Animal Science
Y. Tian, X. Shen, J. Zhao, Y. Wei, S. Han, H. Yin
Summary: This study found that circular RNA circSUCO may promote chicken skeletal muscle development by competitively binding with miR-15a, miR-15b-5p, and miR-15c-5p.
BRITISH POULTRY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Nunzia D'Onofrio, Francesco Prattichizzo, Elisa Martino, Camilla Anastasio, Luigi Mele, Rosalba La Grotta, Celestino Sardu, Antonio Ceriello, Raffaele Marfella, Giuseppe Paolisso, Maria Luisa Balestrieri
Summary: miR-27b is highly expressed in endothelial cells and plays a crucial role in regulating inflammatory pathways, cell cycle, apoptosis, and mitochondrial oxidative imbalances. miR-27b mimic can counteract the cytotoxicity and inflammation caused by TNF-alpha, as well as restore mitochondrial function. Mechanistically, miR-27b targets FOXO1 to achieve its functions.
Article
Biochemistry & Molecular Biology
Xiaoyu Qiu, Guangliang Gao, Lei Du, Jing Wang, Qi Wang, Feiyun Yang, Xiaorong Zhou, Dingbiao Long, Jinxiu Huang, Zuohua Liu, Renli Qi
Summary: This study investigated the regulatory roles and mechanisms of lncRNAs during adipogenic transdifferentiation in muscle cells. The results showed significant changes in the expression of lncRNAs and mRNAs at different time points during the adipogenic differentiation of skeletal muscle satellite cells (SMSCs). Enrichment analysis revealed that DE mRNAs with increased expressions were mainly involved in pathways related to lipid metabolism and fat cell differentiation. Furthermore, several lncRNAs may target specific genes in the fatty acid binding protein (FABP) family and regulate their expressions. These findings provide new insights into the molecular mechanisms of transdifferentiation and adipogenesis.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Pengcheng Lyu, Robert E. Settlage, Honglin Jiang
Summary: Tens of thousands of H3K27ac-marked active enhancers have been identified from bovine satellite cells before or during differentiation. These enhancers contain binding sites not only for transcription factors whose role in satellite cell differentiation is well known but also for transcription factors whose role in satellite cell differentiation is unknown. These enhancers and transcription factors are valuable resources for understanding the complex mechanism that mediates gene expression during satellite cell differentiation.
Article
Agriculture, Dairy & Animal Science
Lu Xiang, Zhiqing Huang, Xiaoling Chen, Gang Jia, Guangmang Liu, Hua Zhao
Summary: The study reveals that leucine promotes muscle fiber type transformation from fast-twitch to slow-twitch through the AdipoQ signaling pathway. This is achieved by increasing the expression of slow muscle markers and decreasing the expression of fast muscle markers. Leucine plays a significant role in regulating muscle fiber type transformation.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hiroyuki Iio, Tadahiko Kikugawa, Yuichiro Sawada, Hiroshi Sakai, Shuhei Yoshida, Yuta Yanagihara, Aoi Ikedo, Noritaka Saeki, So-ichiro Fukada, Takashi Saika, Yuuki Imai
Summary: Dnmt1 plays a crucial role in skeletal muscle regeneration, as indicated by the significant decrease in muscle weight and cell number in Dnmt1 knockout mice. RNA sequencing analysis revealed alterations in gene expression related to cell adhesion and apoptosis in satellite cells from knockout mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Nutrition & Dietetics
Muhammad Subhan Alfaqih, Vita Murniati Tarawan, Nova Sylviana, Hanna Goenawan, Ronny Lesmana, Susianti Susianti
Summary: Vitamin D has effects on satellite cells, promoting proliferation in early stages but impairing skeletal muscle regeneration in high doses. Supplementation of vitamin D may help maintain satellite cell function in conditions like aging and chronic disease.
Article
Biochemistry & Molecular Biology
Yan Zhu, Jianfeng Ma, Hongmei Pan, Mailin Gan, Linyuan Shen
Summary: MicroRNAs (miRNAs) were identified in the skeletal muscle of normal and intrauterine growth retardation (IUGR) neonatal piglets using sequencing. The differentially expressed miRNAs were found to be involved in various signaling pathways and metabolic processes. The miR-29 family was identified as an essential regulator in IUGR pigs, affecting cell proliferation and protein degradation.
Article
Biochemistry & Molecular Biology
Xiong Wang, Fan Liu, Qin An, Wenli Wang, Zhimei Cheng, Yunping Dai, Qingyong Meng, Yali Zhang
Summary: Lactoferrin (Ltf) plays a protective role in skeletal muscle by promoting the proliferation of satellite cells (SCs) and skeletal muscle regeneration. It holds promise as a novel therapeutic agent for skeletal muscle injuries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Linhui Zhu, Lianjie Hou, Jinxin Ou, Guli Xu, Fangyi Jiang, Chingyuan Hu, Chong Wang
Article
Agriculture, Multidisciplinary
Hong Quyen Dang, Xu Gu-li, Hou Lian-jie, Xu Jian, Hong Guang-liang, Chingyuan Hu, Wang Chong
JOURNAL OF INTEGRATIVE AGRICULTURE
(2020)
Article
Cell Biology
Jiaxin Qiao, Shanshan Wang, Jian Zhou, Baohua Tan, Zicong Li, Enqin Zheng, Gengyuan Cai, Zhenfang Wu, Linjun Hong, Ting Gu
Summary: This study revealed that the ITGB6 gene is regulated by H3K27me3 during muscle development in pigs and has an inhibitory effect on the proliferation of porcine skeletal muscle satellite cells.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Xupeng Zang, Ting Gu, Wenjing Wang, Chen Zhou, Yue Ding, Shengchen Gu, Zhiqian Xu, Yanshe Xie, Zicong Li, Gengyuan Cai, Bin Hu, Linjun Hong, Zhenfang Wu
Summary: Spontaneous abortion (SAB) in porcine pregnancy is a major concern in commercial pig farming worldwide. This study used high-throughput sequencing and bioinformatics tools to analyze healthy and arresting endometrium on day 28 of pregnancy, revealing an association between SAB events and the regulation of immunobiological processes, and constructing a specific molecular regulatory network.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zhang Mingjun, Mo Fei, Xu Zhousong, Xu Wei, Xu Jian, Yi Yuanxue, Shen Youfeng, Chen Zhongping, Long Yiqin, Zhao Xiaohong, Cheng Ying, Wang Zhenbing, Deng Zehu, Li Lanjuan
Summary: This study compared the differences in intestinal flora and CD4 T cells between HIV patients and healthy subjects using sequencing methods. The results showed that HIV patients had lower alpha diversity indices of intestinal flora and specific bacterial species compared to the healthy group. Correlation analysis also revealed the relationship between different bacterial populations and the contents of IL-2, IL-8, and TNF-alpha.
Article
Oncology
Yuanxue Yi, Qiufang Zhang, Youfeng Shen, Yueyi Gao, Xiaohong Fan, Sini Chen, Xin Ye, Jian Xu
Summary: This study explored the association of the AP1M2 gene with the survival, immune invasion, and tumor neoantigens of patients with various cancers. AP1M2 was found to be highly expressed in invasive breast carcinoma and positively correlated with patient outcomes and immune infiltration.
JOURNAL OF ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Xiao Wu, Huaxing Zhao, Junkun Lai, Ning Zhang, Junsong Shi, Rong Zhou, Qiaoyun Su, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Zhenfang Wu, Zicong Li
Summary: IL17D protein enhances the developmental ability of cloned pig embryos by inhibiting cell death and up-regulating GADD45B expression; it also reduces cell apoptosis and promotes gene expression in cloned pig embryos, improving the efficiency of somatic cell nuclear transfer embryos.
Review
Biochemistry & Molecular Biology
Jianyu Gan, Ting Gu, Huaqiang Yang, Zheng Ao, Gengyuan Cai, Linjun Hong, Zhenfang Wu
Summary: This review discusses the function and mechanism of micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in regulating spontaneous abortion (SA). It suggests the existence of a competitive endogenous RNA (ceRNA) regulatory network in the onset and development of SA. Understanding this network is important for the search of potential RNA markers for SA diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Xiaohui Yang, Chuanzhao Yu, Qiuyan Zhang, Linjun Hong, Ting Gu, Enqin Zheng, Zheng Xu, Zicong Li, Changxu Song, Gengyuan Cai, Zhenfang Wu, Huaqiang Yang
Summary: This study generated mutant mice with resistance to pseudorabies virus (PRV) by introducing a single amino acid substitution. The mutant mice showed alleviated disease manifestations, decreased death rate, and reduced viral loading in serum and tissue compared to wild-type mice. However, the mutation also caused a defect in eye development.
Article
Immunology
Youfeng Shen, Jian Xu, Shenshen Zhi, Wenyan Wu, Yingying Chen, Qiang Zhang, Yan Zhou, Ze Deng, Wei Li
Summary: This study found that MIP can influence the phagocytic and chemotactic activities of RAW264.7 macrophages through the axis of lncRNA GAS5/miR-21/SOCS6.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shengchen Gu, Xupeng Zang, Lei Jiang, Ting Gu, Fanming Meng, Sixiu Huang, Gengyuan Cai, Zicong Li, Zhenfang Wu, Linjun Hong
Summary: In this study, we investigated the miRNA expression profile in healthy and arresting embryo implantation site of porcine endometrium on day 28 of gestation using small RNA sequencing. We identified differentially expressed miRNAs and predicted their target genes. Furthermore, we confirmed the binding of miR-205 to tubulointerstitial nephritis antigen-like 1 (TINAGL1) using fluorescence in situ hybridization and a dual-luciferase report gene assay. Our findings suggest that miR-205 inhibits angiogenesis in the maternal endometrium, leading to the formation of arresting embryos during implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Fang Zeng, Sha Liao, Zhe Kuang, Qingchun Zhu, Hengxi Wei, Junsong Shi, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Stefan Moisyadi, Johann Urschitz, Zicong Li, Zhenfang Wu
Summary: Farm animals' salivary glands have the potential to efficiently produce human therapeutic proteins. This study successfully used transgenic pigs to synthesize and purify biologically active human nerve growth factor (hNGF) from their salivary glands. The method employed the replacement of the endogenous gene to avoid contamination during purification. This research is significant for human health and biomedicine.
Article
Biochemistry & Molecular Biology
Yongzhong Wang, Chen Zhou, Fanming Meng, Qun Hu, Yue Ding, Xiaoliang Wang, Ting Gu, Zicong Li, Zhenfang Wu, Linjun Hong, Gengyuan Cai
Summary: miR-92b-3p modulates embryo implantation by promoting the proliferation and migration of PTr2 cells, which in turn affects the outcome of gestation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Yue Ding, Qun Hu, Jianyu Gan, Xupeng Zang, Ting Gu, Zhenfang Wu, Gengyuan Cai, Linjun Hong
Summary: Researchers have discovered that ssc-miR-143-3p in extracellular vesicles of porcine uterine luminal fluid affects the proliferation and migration of porcine trophoblast cells by mediating GPD2, thereby playing an important role in embryo implantation.
Article
Agriculture, Dairy & Animal Science
Junsong Shi, Baohua Tan, Lvhua Luo, Zicong Li, Linjun Hong, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Ting Gu