期刊
CELLULAR MICROBIOLOGY
卷 20, 期 9, 页码 -出版社
WILEY
DOI: 10.1111/cmi.12860
关键词
antifungal gene; C. albicans super-infection; Setdb2; systemic bacterial infection
资金
- National Natural Science Foundation of China [81201250]
- National Key Technology Support Program [2012BAI11B05]
Systemic bacterial infections are prone to secondary Candida albicans super-infection. However, the molecular mechanisms involved remain poorly understood. In this study, a model comprising sublethal cecal ligation and puncture plus C.albicans intravenous injection was applied to mimic the situation in super-infection. Compared with mice without systemic bacterial infection, mice with systemic bacterial infection had lower antifungal gene expression (including Il1b, Tnf, Il6, Ifnb, Ifng, Cxcl1, and Ccr2) in monocytes and less inflammatory monocytes and neutrophils infiltrating into the kidney when challenged with C.albicans. Further, lentivirus-mediated Setdb2-knockout and overexpression experiments verified that Setdb2 levels in monocytes correlated negatively with antifungal gene expression and survival rates. Transcriptional repression was probably achieved by Setdb2 through H3 methylation at lysine 9 in promoter regions of these antifungal genes.
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