Article
Cell & Tissue Engineering
Nicolas Dray, Laure Mancini, Udi Binshtok, Felix Cheysson, Willy Supatto, Pierre Mahou, Sebastien Bedu, Sara Ortica, Emmanuel Than-Trong, Monika Krecsmarik, Sebastien Herbert, Jean-Baptiste Masson, Jean-Yves Tinevez, Gabriel Lang, Emmanuel Beaurepaire, David Sprinzak, Laure Bally-Cuif
Summary: The study shows that neural stem cells coordinate their decision to divide in the adult zebrafish brain using spatiotemporally resolved local feedback signals, including short-range inhibition from transient neural progenitors and a dispersion effect from the dividing NSCs themselves. These signals are linked by lineage progression and control the spatiotemporal distribution of output neurons, generating self-propagating dynamics that maintain NSC population homeostasis.
Article
Cell Biology
Fabian L. Cardenas-Diaz, Derek C. Liberti, John P. Leach, Apoorva Babu, Jonathan Barasch, Tian Shen, Maria A. Diaz-Miranda, Su Zhou, Yun Ying, Danielle A. Callaway, Michael P. Morley, Edward E. Morrisey
Summary: By using single-cell transcriptomics, tissue damage segmentation, and mouse lineage tracing, we found that Tfcp2l1 is a regulator of lung alveolus regeneration. Loss of Tfcp2l1 inhibits self-renewal and enhances AT2-AT1 differentiation in adult AT2 cells. Tfcp2l1 temporally regulates AT2 self-renewal and differentiation in alveolar regions undergoing active regeneration.
Article
Multidisciplinary Sciences
Cheng Jin, Zhipeng Wang, Pengyu Li, Jielin Tang, Tao Jiao, Yiran Li, Jinhuan Ou, Dingfeng Zou, Mengzhen Li, Xinyu Mang, Jun Liu, Yanni Ma, Xiaolong Wu, Jie Shi, Shitao Chen, Manman He, Yan Lu, Ning Zhang, Shiying Miao, Fei Sun, Linfang Wang, Kai Li, Jia Yu, Wei Song
Summary: This study investigates the intercellular interactions between spermatogonial stem cell (SSC) and testicular niche in both adult mice and humans, uncovering the crucial role of C-C motif chemokine ligand 24 and insulin-like growth factor binding protein 7 in maintaining SSC homeostasis. The clinical relevance of specific paracrine factors in human fertility is also established.
Article
Multidisciplinary Sciences
Pengfei Guo, Nam Hoang, Joseph Sanchez, Elaine H. Zhang, Keshari Rajawasam, Kristiana Trinidad, Hong Sun, Hui Zhang
Summary: The authors demonstrate that the assembly/disassembly of mammalian SWI/SNF complexes is dynamically regulated by a lysine methylation mechanism involving LSD1 demethylase and L3MBTL3 methyl-lysine reader protein. This mechanism targets SET7-methylated lysines in SMARCC1 and SMARCC2 for proteolysis by CRL4 ubiquitin ligase. The study also reveals the critical role of LSD1 and L3MBTL3 in maintaining the pluripotency and self-renewal of embryonic stem cells, as well as regulating proteolysis induced by loss of SWI/SNF subunits.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Shuai Ma, Si Wang, Yanxia Ye, Jie Ren, Ruiqing Chen, Wei Li, Jiaming Li, Liyun Zhao, Qian Zhao, Guoqiang Sun, Ying Jing, Yuesheng Zuo, Muzhao Xiong, Yuanhan Yang, Qiaoran Wang, Jinghui Lei, Shuhui Sun, Xiao Long, Moshi Song, Shuyang Yu, Piu Chan, Jianwei Wang, Qi Zhou, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Guang-Hui Liu
Summary: This study provides a comprehensive framework to explore aging and rejuvenating trajectories at single-cell resolution, revealing cellular and molecular programs that instruct systemic revitalization by blood-borne factors. Specifically, it identified hematopoietic stem and progenitor cells (HSPCs) as one of the most responsive cell types to young blood exposure, initiating a continuum of cell state changes and restoring a youthful transcriptional regulatory program and cell-cell communications in HSPCs.
Article
Biotechnology & Applied Microbiology
Guowen Hu, Yuguo Xia, Bi Chen, Juntao Zhang, Liangzhi Gong, Yu Chen, Qing Li, Yang Wang, Zhifeng Deng
Summary: The study demonstrates that neural stem cell senescence can lead to cognitive impairment, and treatment with ESC-sEVs in mice models can effectively reverse this process by rejuvenating the senescent stem cells, improving regeneration capacity, and ameliorating cognitive deficits.
Article
Biology
Nannan Guo, Kelsey D. McDermott, Yu-Tzu Shih, Haley Zanga, Debolina Ghosh, Charlotte Herber, William R. Meara, James Coleman, Alexia Zagouras, Lai Ping Wong, Ruslan Sadreyev, J. Tiago Goncalves, Amar Sahay
Summary: This study reveals that the transcription factor Klf9 functions as a brake on symmetric self-renewal of radial-glial neural stem cells (RGLs) in mice. The loss of Klf9 promotes the activation state of RGLs and leads to increased symmetric self-renewal. The study also provides molecular insights into the genetic programs underlying RGL symmetric self-renewal, including Notch and mitogen signaling, cell cycle, fatty acid oxidation, and lipogenesis.
Article
Cell & Tissue Engineering
Marina Sanchez-Petidier, Consuelo Guerri, Victoria Moreno-Manzano
Summary: This study demonstrates the important role of Toll-like receptors (TLRs) in neural precursor cells (NPCs) of the spinal cord. TLRs play distinct regulatory roles in maintaining the self-renewal and cell differentiation of NPCs.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Shanshan Chen, Wenqi Wang, Hor-Yue Tan, Yuanjun Lu, Zhiping Li, Yidi Qu, Ning Wang, Di Wang
Summary: Autophagy is crucial for cellular homeostasis and senescence, with dysregulation implicated in various human diseases. This review highlights the role of autophagy in stem cell maintenance, focusing on its interaction with adult stem cells in disease initiation and progression. Understanding the mechanisms of autophagy in maintaining stemness may provide insights into disease pathogenesis and therapy development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Weam S. Shahin, Shima O. Ebed, Scott R. Tyler, Branko Miljkovic, Soon H. Choi, Yulong Zhang, Weihong Zhou, Idil A. Evans, Charles Yeaman, John F. Engelhardt
Summary: This study reveals that reactive oxygen species regulate the behavior of neuronal stem cells by oxidizing cysteine residues on Igfbp2 protein, thus controlling their self-renewal and lineage commitment.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Asahi Nakamuta, Kana Yoshido, Honda Naoki
Summary: This study developed a mathematical model called the hierarchical neutral competition (hNC) model, which integrates the characteristics of both the classical model and the recent model. The model was validated using experimental data and it predicts the spontaneous generation of clonal bursts in primate hematopoiesis. The findings have significant implications for understanding the maintenance and differentiation of stem cells in tissues.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Gustavo Ulises Martinez-Ruiz, Abigail Morales-Sanchez, Angel Francisco Pacheco-Hernandez
Summary: The text highlights the relationship between the presence of cancer stem cells and worsened clinical outcomes, as well as the role of YY1 in regulating the phenotype of cancer stem cells.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Neurosciences
Zhao-Zhe Hao, Jia-Ru Wei, Dongchang Xiao, Ruifeng Liu, Nana Xu, Lei Tang, Mengyao Huang, Yuhui Shen, Changsheng Xing, Wanjing Huang, Xialin Liu, Mengqing Xiang, Yizhi Liu, Zhichao Miao, Sheng Liu
Summary: The study used single-cell RNA sequencing and culturing techniques to investigate adult neurogenesis in the macaque hippocampus. The analysis revealed the presence of various neural precursor cell populations and the processes involved in adult neurogenesis. This study provides a comprehensive atlas of adult neurogenesis in primates.
NATURE NEUROSCIENCE
(2022)
Article
Cell Biology
Xuejing Ren, Hang Zhao, Lin Shi, Zhengran Li, Ruiyan Kong, Rui Ma, Lemei Jia, Shan Lu, Jian-Hua Wang, Meng-Qiu Dong, Yingchun Wang, Zhouhua Li
Summary: Stem cell proliferation is regulated by phosphorylation, and the interdependence of phosphorylation sites affects the function of Yun in intestinal stem cell proliferation.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Sarbani Saha, Rumela Bose, Shreeta Chakraborty, Rupasri Ain
Summary: This study reveals the essential role of Cox6B2 in maintaining the stemness of trophoblast stem cells and its regulation by various factors including other genes and microRNAs. These findings have important implications for understanding stem cell biology and placental growth.
Article
Anatomy & Morphology
M. P. Madrigal, B. Ballester-Lurbe, O. Gomez, J. A. Moreno-Bravo, E. Puelles, S. Jurado, J. M. Garcia-Verdugo, I. Perez-Roger, Jose Terrado
Summary: Rnd3 is a protein involved in oligodendrocyte maturation, with potential roles in the development of the central nervous system and demyelinating diseases. Its absence leads to hypomyelination, reduced oligodendrocyte numbers, and thinner myelin sheaths in mice, indicating its crucial role in myelination.
BRAIN STRUCTURE & FUNCTION
(2022)
Article
Neurosciences
Daniel Esteve, Maria Micaela Molina-Navarro, Esther Giraldo, Noelia Martinez-Varea, Mari-Carmen Blanco-Gandia, Marta Rodriguez-Arias, Jose Manuel Garcia-Verdugo, Jose Vina, Ana Lloret
Summary: Neurogenesis and neuronal migration in the adult brain are impaired in Alzheimer's disease, leading to cell senescence and reduced odor discrimination ability. Studying the APP/PS1 Alzheimer's mouse model may provide insights for new Alzheimer's treatments based on senolytic compounds or pro-neurogenic factors.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Paula Garcia-Belda, Helena Prima-Garcia, Alicia Aliena-Valero, Maria Castello-Ruiz, Maria Jose Ulloa-Navas, Amadeo Ten-Esteve, Luis Marti-Bonmati, Juan B. Salom, Jose Manuel Garcia-Verdugo, Sara Gil-Perotin
Summary: Mesenchymal stem cell therapy after stroke shows promise, but the viability of cells may be compromised.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mercedes F. Paredes, Cristina Mora, Quetzal Flores-Ramirez, Arantxa Cebrian-Silla, Ashley Del Dosso, Phil Larimer, Jiapei Chen, Gugene Kang, Susana Gonzalez Granero, Eric Garcia, Julia Chu, Ryan Delgado, Jennifer A. Cotter, Vivian Tang, Julien Spatazza, Kirsten Obernier, Jaime Ferrer Lozano, Maximo Vento, Julia Scott, Colin Studholme, Tomasz J. Nowakowski, Arnold R. Kriegstein, Michael C. Oldham, Andrea Hasenstaub, Jose Manuel Garcia-Verdugo, Arturo Alvarez-Buylla, Eric J. Huang
Summary: Research has revealed that the human embryonic ventral forebrain contains nests of proliferative neuroblasts which play a crucial role in generating a sufficient number of functional interneurons for the human brain.
Editorial Material
Multidisciplinary Sciences
Arturo Alvarez-Buylla, Arantxa Cebrian-Silla, Shawn F. Sorrells, Marcos Assis Nascimento, Mercedes F. Paredes, Jose Manuel Garcia-Verdugo, Zhengang Yang, Eric J. Huang
Summary: This study lacks evidence to support the link between proliferating cells and neurogenesis, the role of multipolar nestin(+) astrocytes as progenitors, and the identity of mature-looking doublecortin+ neurons as adult-born. The histology-marker expression observed differs from species with well-documented adult hippocampal neurogenesis.
Article
Clinical Neurology
Raquel Baviera-Munoz, Lidon Carretero-Vilarroig, Nuria Muelas, Rafael Sivera, Pablo Sopena-Novales, Begona Martinez-Sanchis, Isabel Sastre-Bataller, Marina Campins-Romeu, Irene Martinez-Torres, Jose Manuel Garcia-Verdugo, Jose M. Millan, Teresa Jaijo, Elena Aller, Luis Bataller
Summary: SCA36 is a common cause of hereditary ataxia in Eastern Spain, with a strong founder effect. Its clinical features include hypoacusis, pyramidal signs, lingual fasciculations/atrophy, dystonia, and parkinsonism. SCA36 analysis should be considered as a priority in the study of hereditary ataxia, especially in patients with late onset.
MOVEMENT DISORDERS CLINICAL PRACTICE
(2023)
Article
Medicine, Research & Experimental
Laura Martinez-Ruiz, Javier Florido, Cesar Rodriguez-Santana, Alba Lopez-Rodrigueza, Ana Guerra-Librero, Beatriz I. Fernandez-Gild, Patricia Garcia-Tarraga, Jose Manuel Garcia-Verdugo, Felix Oppel, Holger Sudhoff, David Sanchez-Porras, Amadeo Ten-Steve, Jose Fernandez-Martinez, Pilar Gonzalez-Garcia, Iryna Rusanova, Dario Acuna-Castroviejoa, Victor Carriel, Germaine Escames
Summary: The study investigates the mechanisms for increasing melatonin bioavailability in head and neck squamous cell carcinoma (HNSCC) and its potential as an adjuvant to improve cisplatin therapeutic efficacy. The results demonstrate that intratumoral injection of melatonin significantly inhibits tumor progression and enhances the effects of cisplatin.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Neurosciences
Alba Ortega-Gasco, Antoni Parcerisas, Keiko Hino, Vicente Herranz-Perez, Fausto Ulloa, Alba Elias-Tersa, Miquel Bosch, Jose Manuel Garcia-Verdugo, Sergi Simo, Lluis Pujadas, Eduardo Soriano
Summary: NCAM2 plays a significant role in the regulation of RGPs during adult neurogenesis and corticogenesis.
Article
Biochemistry & Molecular Biology
Alejandra Suarez-Carrillo, Monica Alvarez-Cordoba, Ana Romero-Gonzalez, Marta Talaveron-Rey, Suleva Povea-Cabello, Paula Cilleros-Holgado, Rocio Pinero-Perez, Diana Reche-Lopez, David Gomez-Fernandez, Jose Manuel Romero-Dominguez, Manuel Munuera-Cabeza, Antonio Diaz, Susana Gonzalez-Granero, Jose Manuel Garcia-Verdugo, Jose A. Sanchez-Alcazar
Summary: In this study, autophagic defects and secondary pathological consequences were demonstrated in cellular models derived from patients with WDR45 mutations in beta-propeller protein-associated neurodegeneration (BPAN). The study showed that WDR45 mutations impaired autophagy, iron metabolism, and cell bioenergetics. While antioxidants partially improved cellular physiopathology, autophagy and cell bioenergetics remained affected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Li Wang, Kaifang Pang, Li Zhou, Arantxa Cebrian-Silla, Susana Gonzalez-Granero, Shaohui Wang, Qiuli Bi, Matthew L. White, Brandon Ho, Jiani Li, Tao Li, Yonatan Perez, Eric J. Huang, Ethan A. Winkler, Mercedes F. Paredes, Rothem Kovner, Nenad Sestan, Alex A. Pollen, Pengyuan Liu, Jingjing Li, Xianhua Piao, Jose Manuel Garcia-Verdugo, Arturo Alvarez-Buylla, Zhandong Liu, Arnold R. Kriegstein
Summary: This study generates a cross-species proteomic map of synapse development in the neocortex of human, macaque and mouse. The results show that human PSDs mature slower than other species and contain higher levels of Rho guanine nucleotide exchange factors in the perinatal period. In addition, PSD proteins can be divided into four modules that exert stage- and cell-type-specific functions. The findings provide insights into the molecular basis and evolutionary changes of synapse maturation.
Article
Cell Biology
Kimberly K. Hoi, Wenlong Xia, Ming Ming Wei, Maria Jose Ulloa Navas, Jose-Manuel Garcia Verdugo, Maxence Nachury, Jeremy F. Reiter, Stephen P. J. Fancy
Summary: Remyelination after white matter injury (WMI) often fails in diseases like multiple sclerosis due to improper recruitment and repopulation of oligodendrocyte precursor cells (OPCs) in lesions. Primary cilia play a crucial role in the OPC response to WMI through the GPCR/cAMP/CREB signaling axis.
Article
Multidisciplinary Sciences
Vicente Herranz-Perez, Jin Nakatani, Masaki Ishii, Toshiaki Katada, Jose Manuel Garcia-Verdugo, Shinya Ohata
Summary: This study found the peak expression of ZFTA in mouse embryos and observed that ZFTA may be regulated by ciliary transcription factors. However, ZFTA is not essential for the development or flow of supratentorial ependymoma.
SCIENTIFIC REPORTS
(2022)