Article
Engineering, Environmental
Ying Zhang, Yaqi Jiao, Yue Tao, Zixu Li, Hui Yu, Siyue Han, Yang Yang
Summary: Monobutyl phthalate (MBP) can cause acute effects on zebrafish liver, including endoplasmic reticulum stress, lipid synthesis promotion, lipid droplet accumulation, and inhibition of glucose metabolism, leading to cell death and liver damage.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Cell Biology
Inna Rabinovich-Nikitin, Mina Rasouli, Cristine J. Reitz, Illana Posen, Victoria Margulets, Rimpy Dhingra, Tarak N. Khatua, James A. Thliveris, Tami A. Martino, Lorrie A. Kirshenbaum
Summary: This study reveals that the CLOCK gene transcriptionally coordinates mitochondrial quality control mechanisms in cardiac myocytes, regulating an adaptive stress response crucial for cell survival. Restoring CLOCK activity may be beneficial in reducing cardiac injury in individuals with disrupted circadian rhythms.
Review
Biochemistry & Molecular Biology
Katrin Panzitt, Martin Wagner
Summary: The liver serves as a central metabolic hub that coordinates nutritional inputs and metabolic outputs. FXR in the liver and intestine plays a crucial role in regulating postprandial nutrient disposal. Aside from classical roles, FXR also has effects on amino acid, protein metabolism, autophagic turnover, and inflammation, which are less studied. Additionally, understanding of how FXR signaling is affected by posttranslational modifications and different isoforms is important for potential pharmaceutical targeting in clinical applications.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Cell Biology
Shogo Sato, Tomoaki Hishida, Kenichiro Kinouchi, Fumiaki Hatanaka, Yumei Li, Quy Nguyen, Yumay Chen, Ping H. Wang, Kai Kessenbrock, Wei Li, Juan Carlos Izpisua Belmonte, Paolo Sassone-Corsi
Summary: Distinct metabolic conditions in pluripotent stem cells (PSCs) are connected to clock functions through the circadian repressor CRY1. Metabolic reprogramming and the accumulation of CRY1 are essential for self-renewal capacity, colony organization, and metabolic signatures in PSCs. CRY1 regulates developmental and differentiation genes in PSCs, and its absence impairs induced PSC (iPSC) reprogramming efficiency.
Article
Veterinary Sciences
Xinbo Wang, Yuming Zhang, Yansong Li, Mingyu Tang, Qinghua Deng, Jingdong Mao, Liyin Du
Summary: E2 promotes glycogen storage in PMNs by enhancing glucose uptake, retarding glycolysis, and inducing autophagy, inhibiting apoptosis in response to low glucose levels, thus maintaining the survival and immune potential of PMNs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Endocrinology & Metabolism
Ye Young Kim, Hagoon Jang, Gung Lee, Yong Geun Jeon, Jee Hyung Sohn, Ji Seul Han, Won Taek Lee, Jeu Park, Jin Young Huh, Hahn Nahmgoong, Sang Mun Han, Jeesoo Kim, Minwoo Pak, Sun Kim, Jong-Seo Kim, Jae Bum Kim
Summary: This study demonstrated that the reduction of hepatic CRY1 protein in diabetic mice is stimulated by FBXL3-dependent proteasomal degradation and GSK3 beta-induced CRY1 phosphorylation. Tight regulation of hepatic CRY1 protein stability is crucial for maintaining systemic glucose homeostasis.
Article
Biochemistry & Molecular Biology
Sibel Cal-Kayitmazbatir, Eylem Kulkoyluoglu-Cotul, Jacqueline Growe, Christopher P. Selby, Seth D. Rhoades, Dania Malik, Hasimcan Oner, Hande Asimgil, Lauren J. Francey, Aziz Sancar, Warren D. Kruger, John B. Hogenesch, Aalim Weljie, Ron C. Anafi, Ibrahim Halil Kavakli
Summary: The study reveals a functional interaction between cystathionine beta-synthase (CBS) and the core circadian protein cryptochrome 1 (CRY1), impacting circadian rhythms and metabolic health. CRY1 modulates CBS activity, influencing both circadian physiology and metabolic pathways. The CBS-CRY1 binding serves as a post-translational switch to regulate cellular circadian physiology and metabolic control.
Article
Chemistry, Multidisciplinary
Dusan Kolarski, Simon Miller, Tsuyoshi Oshima, Yoshiko Nagai, Yugo Aoki, Piermichele Kobauri, Ashutosh Srivastava, Akiko Sugiyama, Kazuma Amaike, Ayato Sato, Florence Tama, Wiktor Szymanski, Ben L. Feringa, Kenichiro Itami, Tsuyoshi Hirota
Summary: CRY1 and CRY2 proteins are essential components of the circadian clock. Developing CRY1-selective compounds that can be manipulated through light-dependent mechanisms allows for a better understanding of the specific functions of CRY1 and CRY2. By utilizing benzophenone derivatives to interact with the photo lyase region of CRY1, researchers were able to activate CRY1 without affecting CRY2, demonstrating a potential for spatiotemporal regulation of CRY1 activity for health and disease research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Gastroenterology & Hepatology
Maria J. Gonzalez-Rellan, Eva Novoa, Natalia da Silva Lima, Amaia Rodriguez, Christelle Veyrat-Durebex, Samuel Seoane, Begona Porteiro, Marcos F. Fondevila, Uxia Fernandez, Marta Varela-Rey, Ana Senra, Cristina Iglesias, Adriana Escudero, Miguel Fidalgo, Diana Guallar, Roman Perez-Fernandez, Vincent Prevot, Markus Schwaninger, Miguel Lopez, Carlos Dieguez, Roberto Coppari, Gema Fruhbeck, Ruben Nogueiras
Summary: The transcription factor p63 plays a key role in glucose metabolism by regulating SIRT1 and affecting blood glucose levels, thus influencing glucose homeostasis.
Article
Biochemistry & Molecular Biology
Jose Thalles Lacerda, Patricia R. L. Gomes, Giovanna Zanetti, Nathana Mezzalira, Otoniel G. Lima, Leonardo V. M. de Assis, Ali Guler, Ana Maria Castrucci, Maria Nathalia Moraes
Summary: The transient receptor potential channel vanilloid type 1 (TRPV1) plays an important role in hepatic glucose metabolism, and its loss is associated with increased gluconeogenesis through the PKA signaling pathway. The study provides clues related to TRPV1-associated proteins and metabolic regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Zhenzhen Zi, Zhuzhen Zhang, Qiang Feng, Chiho Kim, Xu-Dong Wang, Philipp E. Scherer, Jinming Gao, Beth Levine, Yonghao Yu
Summary: mTORC1, a central regulator of cell growth and autophagy, is activated on the lysosome surface and phosphorylates STK11IP to regulate lysosomal acidification and autophagy. STK11IP may be a promising therapeutic target for diseases with aberrant autophagy signaling.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ming-Kang Yang, Xiao-Jie Zhu, Chu-Min Chen, Xu Guo, Shu-Xuan Xu, Ya-Rou Xu, Shen-Xiu Du, Shi Xiao, Bernd Mueller-Roeber, Wei Huang, Liang Chen
Summary: Autophagy exhibits circadian rhythms in plants, regulated by the LUX transcription factor. This rhythm is ubiquitous in plants and plays a role in improving plant resistance to carbon starvation.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yue Li, Weidong Wang, Hui-Ying Lim
Summary: Through genetic studies, it was found that the regulation of glucose absorption in the Drosophila midgut is mediated by the Solute Carrier 5A5 (dSLC5A5). Genetically knocking down dSLC5A5 in flies leads to decreased glucose and trehalose levels in the system and circulation, while overexpression of dSLC5A5 increases these levels and promotes glucose uptake in enterocytes. Additionally, it was shown that dSLC5A5 undergoes apical endocytosis in a dynamin-dependent manner, crucial for glucose uptake in enterocytes, and that glucose upregulates dSLC5A5 levels in the midgut, influencing systemic glucose homeostasis on a high-sugar diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yu-Hsuan Chen, Tzu-Yu Huang, Yu-Tung Lin, Shu-Yu Lin, Wen-Hsin Li, Hsiang-Jung Hsiao, Ruei-Liang Yan, Hong-Wen Tang, Zhao-Qing Shen, Guang-Chao Chen, Kuen-Phon Wu, Ting-Fen Tsai, Ruey-Hwa Chen
Summary: The study reveals that branched ubiquitination of VPS34 acts as a switch between UPS and autophagy, playing an important role in lipid metabolism in the liver.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Tiffany Fougeray, Arnaud Polizzi, Marion Regnier, Anne Fougerat, Sandrine Ellero-Simatos, Yannick Lippi, Sarra Smati, Frederic Lasserre, Blandine Tramunt, Marine Huillet, Leonie Dopavogui, Juliette Salvi, Emmanuelle Nedelec, Vincent Gigot, Lorraine Smith, Claire Naylies, Caroline Sommer, Joel T. Haas, Walter Wahli, Helene Duez, Pierre Gourdy, Laurence Gamet-Payrastre, Alexandre Benani, Anne-Francoise Burnol, Nicolas Loiseau, Catherine Postic, Alexandra Montagner, Herve Guillou
Summary: Feeding and insulin play a role in regulating the liver clock and rhythmic gene expression. This study shows that the intact hepatocyte insulin receptor is required for programming the liver clock and associated rhythmic gene expression.
Article
Cardiac & Cardiovascular Systems
Maha Alser, Manjunath Ramanjaneya, Najeha Rizwana Anwardeen, Francesco Donati, Francesco Botre, Jayakumar Jerobin, Ilham Bettahi, Nura Adam Mohamed, Abdul Badi Abou-Samra, Mohamed A. Elrayess
Summary: This study compares the levels of Humanin and MOTS-c in non-athletes and professional athletes. The results showed that professional athletes have lower levels of MOTS-c and higher levels of Humanin. High endurance athletes have lower levels of Humanin. Humanin and MOTS-c levels are highly correlated in athletes.
REVIEWS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Dayamin Martinez-Brito, Patrizia Leogrande, Xavier de la Torre, Francesco Botre
Summary: This study focused on the optimization of a measurement method for thyroid hormones and their derivatives. By adjusting the parameters of the analysis method, such as MRM transitions and ionization source parameters, and selecting appropriate solvents for liquid-liquid extraction and cleanup steps, the accuracy and sensitivity of the analysis were improved. The results showed that the choice of mobile phase additives and the method of enzymatic hydrolysis can affect the stability and accuracy of the analysis results.
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2022)
Article
Psychiatry
Micaela Tirri, Sabrine Bilel, Raffaella Arfe, Giorgia Corli, Beatrice Marchetti, Tatiana Bernardi, Federica Boccuto, Giovanni Serpelloni, Francesco Botre, Fabio De-Giorgio, Krystyna Golembiowska, Matteo Marti
Summary: This study assessed the effects of 25H-NBOMe and its halogenated derivatives in comparison to their analogs and LSD on mice. The results showed that the halogenated derivatives were more effective in altering visual and acoustic responses, affecting reaction time and motor and sensory gating. -NBOMe and analogs not only impacted the reception of sensory stimuli, but also their brain processing.
FRONTIERS IN PSYCHIATRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Mohamed A. Elrayess, Francesco Botre, Amelia Palermo
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Xavier de la Torre, Daniel Jardines, Francesco Botre
Summary: The detection of testosterone and its precursors' abuse in drug testing in sports is based on longitudinal evaluation of markers in the urine. A Bayesian statistical approach is used for credible interval establishment. Additional confirmation by isotope ratio mass spectrometry is conducted on samples showing values outside the acceptable boundaries. The findings suggest that IRMS analysis can be applied to screen a larger number of samples for detection of positive cases.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Basile Moreillon, Olivier Salamin, Bastien Krumm, Loredana Iannella, Francesco Molaioni, Tiia Kuuranne, Raul Nicoli, Jonas J. Saugy, Francesco Botre, Raphael Faiss
Summary: This study investigated the effects of oral contraceptive pills (OCP) on urinary steroid concentrations. The results revealed low levels of testosterone and epitestosterone in urine, potentially due to the interference of OCP. Compared to urine, serum had less variability in steroid concentrations. The findings highlight the importance of using both urine and serum samples for steroid testing in the athlete biological passport (ABP).
DRUG TESTING AND ANALYSIS
(2023)
Article
Physiology
Thomas Zandonai, Ana M. Peiro, Caterina Covelli, Xavier de la Torre, Francesco Botre
Summary: The purpose of this study was to assess the prevalence of tramadol use among athletes from 2012 to 2020. Urine samples collected from national and international in-competition doping-control tests in Italy were analyzed. The results showed that 1.2% of the analyzed samples showed tramadol intake, with the majority coming from cyclists. A negative correlation was found between tramadol use and cycling, suggesting a decrease in tramadol prevalence in this sport.
INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE
(2023)
Article
Biochemical Research Methods
Dayamin Martinez Brito, Teresa Correa Vidal, Xavier de la Torre, Rodny Montes de Oca Porto, Francesco Botre
Summary: The ratio of testosterone (T) to epitestosterone (E) in urine is used as a biomarker to detect testosterone and precursors abuse by athletes. This study investigated different mass spectrometric techniques for correcting the T/E ratio and found that using external calibration samples introduced biases. Applying the corr_2 method improved the accuracy of the T/E calculation by 5-6%, while the use of LC-MSn for measurement produced significantly different results even with the same transition for T and E.
DRUG TESTING AND ANALYSIS
(2023)
Review
Biochemical Research Methods
Bastien Krumm, Jonas J. Saugy, Francesco Botre, Francesco Donati, Raphael Faiss
Summary: The detection of blood doping is a major concern in sports and poses an ongoing challenge for antidoping research. Indirect approaches, such as monitoring specific biomarkers, have been complementing direct detection methods. The identification of markers sensitive to erythropoiesis alteration is essential for screening blood doping.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Dayamin Martinez Brito, Xavier de la Torre, Francesco Botre
Summary: This study investigated the possible changes in the markers of the steroidal module of the athlete biological passport, taking into account athletes' declaration or non-declaration of thyroid hormone supplementation. Concentrations of various biomarkers were calculated using gas chromatography-tandem mass spectrometry, and ratios between these biomarkers were estimated. The results showed significant differences in marker concentrations and ratios between the groups declaring and not-declaring thyroid hormone supplementation. A controlled study confirmed these observations, particularly in the female group. The findings suggest that the interpretation of steroid markers in the athlete biological passport should consider thyroid hormone administrations.
DRUG TESTING AND ANALYSIS
(2023)
Article
Sport Sciences
Thomas Zandonai, Francesco Botre, Maria Gabriella Abate, Ana Maria Peiro, Toby Mundel
Summary: This study assessed the nicotine positivity rate in 60,802 anti-doping urine samples from 2012 to 2020. The results showed that the overall mean positivity rate for nicotine was 22.7%, with higher rates in male samples compared to female samples (24.1% vs 18.5%). Team sports had a higher positivity rate than individual sports (31.4% vs 14.1%). The study calls for WADA and other organizations to address this phenomenon and protect the health and welfare of athletes.
Article
Cell Biology
Nuria Martinez-Lopez, Pamela Mattar, Miriam Toledo, Henrietta Bains, Manu Kalyani, Marie Louise Aoun, Mridul Sharma, Laura Beth J. McIntire, Leslie Gunther-Cummins, Frank P. Macaluso, Jennifer T. Aguilan, Simone Sidoli, Mathieu Bourdenx, Rajat Singh
Summary: Fasting or lipid availability stimulates mTORC2 activity, leading to phosphorylation of NDRG1 and NDRG1-CDC42-mediated mitochondrial fission and respiration.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Bridget Shafit-Zagardo, Simone Sidoli, James E. Goldman, Juwen C. DuBois, John R. Corboy, Stephen M. Strittmatter, Hillary Guzik, Ukuemi Edema, Anita G. Arackal, Yair M. Botbol, Emilio Merheb, Rashed M. Nagra, Sarah Graff
Summary: During inflammatory, demyelinating diseases such as multiple sclerosis (MS), inflammation and axonal damage are prevalent early in the course. This study found that the insoluble protein TMEM106B, normally lysosome-associated, is insoluble in MS plaques relative to normal-appearing white matter from individuals with Alzheimer's disease and non-neurologic controls. Hypomorphic mice with a reduction in TMEM106B have increased axonal damage and lipid droplet accumulation in the spinal cord following experimental autoimmune encephalomyelitis.
Article
Sport Sciences
Giorgia M. Biasini, Francesco Botre, Xavier de la Torre, Francesco Donati
Summary: This study presents a multi-parametric analytical strategy to detect autologous blood transfusions by targeting the modification of red blood cells during storage. The results showed that the irreversible alteration of red blood cells morphology, loss of membrane integrity, occurrence of hemolysis and aging of erythrocytes during storage are closely related to the reduction of certain proteins on the erythrocyte membrane, externalization of phosphatidyl serine, reduced concentration of CD47, and increased levels of erythrocyte microparticles.
SPORTS MEDICINE-OPEN
(2023)
Article
Multidisciplinary Sciences
Zuen Ren, Huizhi Liang, Phillip M. Galbo, Malindrie Dharmaratne, Ameya S. Kulkarni, Atefeh Taherian Fard, Marie Louise Aoun, Nuria Martinez-Lopez, Kimita Suyama, Outhiriaradjou Benard, Wei Zheng, Yang Liu, Joseph Albanese, Deyou Zheng, Jessica C. Mar, Rajat Singh, Michael B. Prystowsky, Larry Norton, Rachel B. Hazan
Summary: In breast cancer, the loss of GPx2 leads to redox imbalance, promoting malignant progression and correlating with key processes such as tumor angiogenesis and metabolic modulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)