Article
Biochemistry & Molecular Biology
Yang Zhou, Yanhua Cui, Chao Suo, Qian Wang, Xiaojun Qu
Summary: The study showed that EPS-M2 produced by Streptococcus thermophilus CS6 exhibited good rheology, thermostability, and antioxidant activity, mainly composed of galactose, arabinose, and glucose. The EPS-M2 has potential applications in the food industry as a natural thickener, stabilizer, and antioxidant agent.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Jun Li, Lianzhong Ai, Feiran Xu, Xintian Hu, Yijun Yao, Lifeng Wang
Summary: In this study, an exopolysaccharide (EPS) produced by Weissella cibaria NC516.11 isolated from distiller grains of Chinese Baijiu was characterized structurally and functionally. The EPS was found to promote the cross-linking of starch molecules and increase water-holding capacity in dough, as well as enhance the viscoelastic properties and gluten network of gluten-free dough.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Longzhan Gan, Guangyang Jiang, Xiaoguang Li, Shihao Zhang, Yongqiang Tian, Biyu Peng
Summary: EPS-B108, a fructose-based exopolysaccharide isolated from halotolerant Bacillus sp. SCU-E108, exhibits no acute oral toxicity, good thermal stability, high solubility in water, and excellent oil-holding capacity. Its properties make it a promising alternative source for functional food additives and ingredients.
Article
Biochemistry & Molecular Biology
Xuan Zhang, Liang Hong, Bao-jie Zhu, Yaozu Yuan, Shao-Ping Li, Jing Zhao
Summary: The purpose of this study was to investigate the morphological characteristics of different brands of lentinan injections produced in China using atomic force microscopy (AFM) and their relationship to immunological activity. The study found that the chain heights of different brands varied, and the immunological activity of Jinling lentinan injection was significantly higher than other brands, with poor consistency in quality.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Wei Xia, Jin Han, Shiming Zhu, Yilin Wang, Wenqing Zhang, Zhengjun Wu
Summary: EPS53, a homogeneous exopolysaccharide (EPS), was isolated from Streptococcus thermophiles XJ53 fermented in skimmed milk and had a high molecular weight and a specific repeating structural unit. EPS53-D, obtained by deproteinization using TCA, had a lower molecular weight but the same repeating structural unit. EPS53 exhibited stronger immune activity than EPS53-D, indicating the importance of molecular weight in EPS activity. TCA treatment may affect the activities of native EPSs produced by fermentation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Puyu Li, Ying Bai, Yuejiao Yang, Mengyuan Li
Summary: This study identified and characterized the extracellular polysaccharides of Streptococcus thermophilus (STEP) and investigated their rheological properties when blended with whey protein (STEP/WP). Two types of polysaccharides, neutral (STEP-1) and acidic (STEP-2), were isolated and purified from S. thermophilus. The molecular weights of STEP-1 and STEP-2 were 2197.74 and 141.99 kDa, respectively. The polysaccharides had different composition, with STEP-1 mainly consisting of galactose, glucose, and rhamnose, and STEP-2 mainly consisting of galactose, mannose, and glucose. Rheological analysis showed that STEP/WP had higher apparent viscosity compared to STEP, and acted as a viscous liquid during dairy processing. These findings have potential applications in the production of fermented milk and cheese.
Article
Food Science & Technology
Elif Tasdelen, Omer Simsek
Summary: Using EPS-producing L. plantarum strains improved the viscosity and color stability of tarhana soups, indicating their potential as starter cultures for enhancing the technological and health aspects of tarhana.
Article
Agriculture, Dairy & Animal Science
Yunchao Wa, Chenchen Zhang, Gulin Sun, Hengxian Qu, Dawei Chen, Yujun Huang, Ruixia Gu
Summary: Increasing the concentrations of histidine, isoleucine, and glutamate can significantly enhance the production of free exopolysaccharide (f-EPS) and the viability of Streptococcus thermophilus cells. This increase is achieved by up-regulating the transcription of EPS biosynthesis genes and enhancing the activity of key enzymes involved in sugar nucleotide synthesis. Furthermore, the higher concentrations of histidine, isoleucine, and glutamate result in increased lactose consumption and decreased galactose secretion, indicating the improved production of f-EPS.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biology
Marion Nabot, Marie Guerin, Dharini Sivakumar, Fabienne Remize, Cyrielle Garcia
Summary: Bacteria can produce different types of homopolysaccharides, and the synthesis of these compounds is influenced by various factors. Controlling the catalyzing enzyme and environmental conditions can guide the production of specific polysaccharides in bacteria, which has applications in improving food texture.
Article
Biochemistry & Molecular Biology
Athmika Nagaraj, Punchappady Devasya Rekha
Summary: Biopolymers are important in tissue engineering, especially in the development of bioinks with suitable properties for printing and supporting cells. Alginate is a widely studied natural biopolymer due to its biocompatibility and ability to mix with other polymers. In this study, an exopolysaccharide called EPS-R17, produced by Rhizobium sp. PRIM17, was incorporated with alginate to create a bioink. The EPS-R17 showed good compatibility with keratinocytes and fibroblasts, and the EPS-R17-alginate bioink exhibited shear thinning behavior and gel-like properties. The bioink also demonstrated good compressive strength and cell viability when used in extrusion bioprinting. These findings highlight the potential of EPS-R17-alginate bioinks for bioprinting applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Swati Tiwari, Digambar Kavitake, Palanisamy Bruntha Devi, Prathapkumar Halady Shetty
Summary: Exopolysaccharides (EPS) are widely used in the food industry, particularly in dairy based products like yoghurt, to enhance stability and texture properties, as well as potentially improving antioxidant and prebiotic functionality. They can also be utilized in symbiotic products and contribute to physical and micro structural stability in yogurt production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Yake Du, Zhen Dai, Tingting Hong, Qing Bi, Haoran Fan, Xueming Xu, Dan Xu
Summary: This study investigated the impact of exopolysaccharides (EPS)-positive strain Weissella cibaria (W. cibaria) fermented sourdough on the quality of whole wheat fresh noodles (WWNs) and its improvement mechanisms. The optimal fermentation conditions were found to be 30% sucrose content, fermented at 25 degrees C for 12 h, which yielded the highest EPS in the sourdough. The results showed that the addition of EPS sourdough delayed browning and improved the texture of WWNs.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Polymer Science
Silvia Baptista, Cristiana A. V. Torres, Chantal Sevrin, Christian Grandfils, Maria A. M. Reis, Filomena Freitas
Summary: In this study, membrane-based methods were evaluated for the recovery of fucose-rich exopolysaccharide (EPS) secreted by Enterobacter A47. The optimized method showed a reduction in water consumption and extraction time, and an increase in product recovery and purity. The changes also improved the emulsion-forming capacity and rheological properties of the biopolymer, which are important for its application in cosmetics, pharmaceuticals, and food products.
Article
Biochemistry & Molecular Biology
Anna Maciejewska, Czeslaw Lugowski, Jolanta Lukasiewicz
Summary: Streptococcus gallolyticus subspecies gallolyticus is a facultative pathogen associated with bacteraemia, infective endocarditis, and sepsis that has been linked with colorectal cancer. Its major sugar antigen, the exopolysaccharide (EPS), is a potential virulence factor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Cheng Liu, Ya-Ping Tseng, Leong-Perng Chan, Chia-Hua Liang
Summary: TCI633, a probiotic isolated from human breast milk, has shown potential to produce hyaluronic acid in the gastrointestinal tract. This study assessed the effect of TCI633 on skin improvement, with results indicating its ability to promote skin cell proliferation, increase collagen content, protect DNA, and inhibit hyaluronidase activities.
JOURNAL OF COSMETIC DERMATOLOGY
(2022)
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)