期刊
CARBOHYDRATE POLYMERS
卷 192, 期 -, 页码 273-281出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.03.013
关键词
Wall polymers; Chemical pretreatment; Lignocellulose hydrolysis; Biomass saccharification; Glycan-directed antibodies; Immunolabeling
资金
- National Science Foundation of China [31670296]
- Fundamental Research Funds for the Central Universities of China [2662015PY018]
- National 111 Project [B08032]
- NSF Plant Genome Program [DBI-0421683]
- NSF [DBI-0421683, RCN 009281]
Miscanthus is a leading bioenergy crop and rice provides enormous biomass for biofuels. Using Calcofluor White staining, this work in situ observed an initial lignocellulose hydrolysis in two distinct Miscanthus accessions, rice cultivar (NPB), and Osfc16 mutant after mild chemical pretreatments. In comparison, the M. sin and Osfc16 respectively exhibited weak Calcofluor fluorescence compared to the M. sac and NPB during enzymatic hydrolysis, consistent with the high biomass saccharification detected in vitro. Using xyloglucan-directed monoclonal antibodies (mAbs), xyloglucan deconstruction was observed from initial cellulose hydrolysis, whereas the M. sin and Osfc16 exhibited relatively strong immunolabeling using xylan-directed mAb, confirming previous findings of xylan positive impacts on biomass saccharification. Furthermore, the M. sin showed quick disappearance of RG-I immunolabeling with varied HG labelings between acid and alkali pretreatments. Hence, this study demonstrated a quick approach to explore wall polymer distinct deconstruction for enhanced biomass saccharification under chemical pretreatment in bioenergy crops.
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